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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: cronicr]
    #24705262 -

All right cool. I appreciate it.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Munchauzen]
    #24706235 -

LogicaL Chaos said:
Heres my question: Have any of u guys grown from a spore print, then continued to grow from spore prints from the previous spore prints, just to see what happens? If so, how many generations did u grow and what happened?


I assume no one responded to this because no one does it? because...  Spores take too long/ more work/ more chance of contams?

Is  it ever useful to add some genetic diversity back into a isolate without going back to the master print/ MS


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"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThunderShroomer]
    #24706412 -

ThunderShroomer said:
Is  it ever useful to add some genetic diversity back into a isolate without going back to the master print/ MS


No. The whole point of isolation is to remove genetics.


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:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
:headbang3: Avoiding the dreaded facepalm in cultivation :headbang3: The worst threads all have the same subjects :headbang3:
:headbang3: Q & A.M.U. :headbang3:

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThunderShroomer]
    #24706631 -

ThunderShroomer said:
LogicaL Chaos said:
Heres my question: Have any of u guys grown from a spore print, then continued to grow from spore prints from the previous spore prints, just to see what happens? If so, how many generations did u grow and what happened?


I assume no one responded to this because no one does it? because...  Spores take too long/ more work/ more chance of contams?

Is  it ever useful to add some genetic diversity back into a isolate without going back to the master print/ MS


Inbreeding  has its ups and downs but general rule being ya want to use your earliest prints possible as they will degrade eventually


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThunderShroomer]
    #24706880 -

I just dunked my BRF cakes, I'm gonna prepare my bulk substrate for tomorrow. I have coir and verm ready, can awesome experienced cultivators tell me how much ratio I should use? (i have been suggested 50-50) and I don't know how much is 'one brick of coir' because my brick is kinda big and I don't really know how much of it I should use. I'm going to boil 4 liters of water and put everything together in a bucket and leave it for the night, is it cool or I should put them in pc? please, tell me what is the best way to do it, i want the best for my mushies (i love my mushies ^_^)


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Know thyself, and thou shalt know the universe and God

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: pezhvak]
    #24706886 -

Id suggest about 80/20 coir to verm and around 10 qts per 66 qt tub and around 20 cakes to spawn it


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: cronicr]
    #24706916 -

I know there's not a concrete answer, but is there a ballpark timeline for how long cube LC and/or LI can be stored longterm without gas exchange? Or a definite, "The myc will die after x amount of time" kind of figure?

Like, I've always read that myc needs GE or it'll stall and die out. That's why we don't close our slant lids all the way down, etc. Is there a general idea for liquids? Will no GE kill off liquid mycelium or suspend it?


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ATTACK OF THE KILLER APE!!!11 | G2G Video | Stare Sticks | Bags in SAB |
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: stareatclouds]
    #24706924 -

You do close off GE on slants all the way after they colonize. Theb store them.


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Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: bodhisatta] * 1
    #24707023 -

Stare I've stored Li for 8 years and had a successful flush. But that was the strongest culture I had. On average id say 3 years. I recently revived a stored stem tissue sample sitting in a syringe in the fridge from 2014 and it fruited very well it wouldn't grow on agar, but it did grow in pf jars. I posted a thread called "how long will this culture last". Decided to put all these old revivals in that thread to see how long I can really push it. Right now I have an LC of oysters from 2007 I put to agar a few days ago. I've grown it out once a year with success. So that what 10 years old? Nature Is amazing and seems to push its own boundaries and adapt.

As for previous questions about forced albinos. Psilocybe fanaticus said he forced albanism with uv light. You can likely find the information somewhere online. But fishy mentioned he used UV light. Haven't heard back about details yet. But he will be back soon enough and if I find out ill let you know. I think the entire grow was done under uv light. I'd assume colonization of bulk sub as well.


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: eatyualive]
    #24707766 -

anything wrong with making a brf and verm mix in advance? if it's okay to how long would it keep?

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: mushroom_tip]
    #24707779 -

I wouldn't make it much more than a few hours in advance. highly nutritious media that's not sterilized will mold up in a day or two


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Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: cronicr]
    #24708341 -

cronicr said:
paegan said:
Hey there, it is my first grow and I have questions about fruiting.

I am using a shotgun terrarium with saturated perlite and grow light 12/12, but didn't realize I needed to elevate the bin off the ground until today. Cakes have been sitting in there for almost a week now (I waited 1 week after 100% colonization before birthing) and I haven't been getting any pinning yet. It smells really "mushroomy" in there and the mycelium has overtaken much of the casing layer and still looks healthy but no shrooms...

What else can I do apart from elevate the bin and mist occasionally to induce fruiting?


Nothing just keep misting and wait!


Thanks cronicr! That is exactly what I have been doing now for the last 3 days. As a follow up:

What is a normal time to wait before I should expect pinning? I placed birthed cakes into sgfc 1.5 weeks ago (didn't mist for the first week :/ ) and I still don't have any clear signs of pins forming...as a matter of fact what exactly should I be looking for, what does the primordia look like when it is just starting out?

I have a ton of small white bumps on a few of the cakes and then I have maybe 3 or 4 much larger and totally different looking bulbous growths on a couple cakes (confused man lol)

Thanks in advance


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Virtual Reality, Analog

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: paegan]
    #24708682 -

I'm about to drill some holes for my monotube, some of the pictures have it in substrate level while others are 5" above the substrate, which one is right and why?


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Know thyself, and thou shalt know the universe and God

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: paegan]
    #24708684 -

I did use the search function but couldn't find an answer, so thought I'd ask here.

I have a good problem of having too many colonized grains for the room and number of tubs I have. How long can I store colonized cubensis grain in the fridge and still have good success with spawning?

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: pezhvak]
    #24709067 -

pezhvak said:
I'm about to drill some holes for my monotube, some of the pictures have it in substrate level while others are 5" above the substrate, which one is right and why?


Because it doesn't really matter that much


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: bodhisatta]
    #24709288 -

awesome! okay, I did my monotube and everything is done. I covered the holes with microtape for colonizing, is it okay to use it in fruiting phase too? or it has to be polyfill?


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Know thyself, and thou shalt know the universe and God

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ShroomyToons]
    #24709310 -

pezhvak said:
awesome! okay, I did my monotube and everything is done. I covered the holes with microtape for colonizing, is it okay to use it in fruiting phase too? or it has to be polyfill?


https://www.shroomery.org/forums/showflat.php/Number/23651523/fpart/all/vc/1

ShroomyToons said:
I did use the search function but couldn't find an answer, so thought I'd ask here.

I have a good problem of having too many colonized grains for the room and number of tubs I have. How long can I store colonized cubensis grain in the fridge and still have good success with spawning?


Long enough, more than months. Probably would still try to use sooner than later


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

Extras: Filter Print Post Top
Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: bodhisatta]
    #24709496 -

Awesome. A month should be long enough for me. Many thanks!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: bodhisatta]
    #24709527 -

awesome, some of the pictures I saw in the link looked like they are not growing as much as the ones used polyfill, is it different in your opinion? (i mean does it have pros and cons?)


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Know thyself, and thou shalt know the universe and God

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ShroomyToons]
    #24709600 -

Can bacteria or other contaminants land on agar and not germinate because of there only being one or two certain unfavorable nutrients, but then when expanded to grains or substrate then the contaminant shows up? Or will anything that lands on the agar start to grow?


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Shop: MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com APE Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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