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 Pastywhyte
Say hello to my little friend
 
Registered: 09/16/12
Posts: 36,621
Loc: Canada 
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 Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer!  [Re: 7even]
#24690802 - 10/07/17 01:58 PM (3 years, 3 months ago) |
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Quote:
BLACKXIII said:
Do you guys find that isolating towards monoculture, away from original ms, sometimes weakens potency? Looking to hear some tc experiences....
Of course. You will see a gamut of results. That's why we test isolates or clones and only keep the good ones. Of course ms is a crap shoot every time.
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7even
deceased
Registered: 02/25/17
Posts: 401
Loc: hell
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Pastywhyte]
#24690833 - 10/07/17 02:15 PM (3 years, 3 months ago) |
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word 
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Munchauzen
Mushroom Maharaja
Registered: 06/23/11
Posts: 13,910
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: 7even]
#24691102 - 10/07/17 04:01 PM (3 years, 3 months ago) |
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Quote:
KidMyco4296 said:
They were elevated. I've just seen videos where people barely use the amount of water I had. Will try to turn the temp down on my second go tonight. Thanks
tape some quarters to your weight. it will increase pressure and decrease water loss. just keep an eye on your temps when you do.
Quote:
BLACKXIII said:
Do you guys find that isolating towards monoculture, away from original ms, sometimes weakens potency? Looking to hear some tc experiences....
its def possible. part of the huge amount of work that is finding a monoculture entails doing many many test grows to find the right genetics.
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 PussyFart
Retired Cultivation Extrodinaire
Registered: 04/08/12
Posts: 22,192
Loc: Orbiting Earth
Last seen: 6 months, 17 days
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: 7even]
#24692738 - 10/08/17 01:23 AM (3 years, 3 months ago) |
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Quote:
BLACKXIII said:
Do you guys find that isolating towards monoculture, away from original ms, sometimes weakens potency? Looking to hear some tc experiences....
it is very possible. Don't think I have ever came across that, but it is definitely possible.
--------------------
  
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
 ^*~.,,.~*^*~.,_,.~*^*~.,_SHIT FOR NOOBS_,.~*^*~.,_,.~*^*~.,_,.~*
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Skelsen
Potato
Registered: 10/03/17
Posts: 39
Last seen: 6 months, 7 days
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: PussyFart]
#24693537 - 10/08/17 12:04 PM (3 years, 3 months ago) |
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Hey!
I am a complete beginner, and just recently started reading seriously about mushrooms. I have my hands on some dried golden teacher.. What would be the best way to try and start a culture from this? I do not know how they were dried.
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Pastywhyte
Say hello to my little friend
Registered: 09/16/12
Posts: 36,621
Loc: Canada
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Skelsen] 1
#24693560 - 10/08/17 12:14 PM (3 years, 3 months ago) |
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Depending on how they were dried you may be able to reanimate them with antibiotic agar. However if they were dried with high heat it would be pointless and even if not it would be a challenge. Personally it would be far easier to just buy a syringe or print.
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Skelsen
Potato
Registered: 10/03/17
Posts: 39
Last seen: 6 months, 7 days
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Pastywhyte]
#24693567 - 10/08/17 12:15 PM (3 years, 3 months ago) |
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Yeah problem about that is not too easy to get in to my country..
Is there any way to tell from how they look, how they were dried?
What kind of antibiotic agar is suggested?
And thank you very much for the answer.
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Pastywhyte
Say hello to my little friend
Registered: 09/16/12
Posts: 36,621
Loc: Canada
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Skelsen] 1
#24693598 - 10/08/17 12:23 PM (3 years, 3 months ago) |
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You can get gentamiacin to make antibiotic agar or buy tetracycline from the pet store. But you cannot pressure cook tetracycline so it needs to be carefully added during the cool down before you pour your plates. It's a bit tricky.
I don't recall hearing about your country banning spores. Should be easy to order some.
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Skelsen
Potato
Registered: 10/03/17
Posts: 39
Last seen: 6 months, 7 days
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Pastywhyte]
#24693653 - 10/08/17 12:42 PM (3 years, 3 months ago) |
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They banned it in 2013.
Anyways thanks very much for the replies.
Think this should be enough to get me googling for the night..
I do not have a pressure cooker at hand, is it essential.
Or is it possible to do this with just steam ?
If it is essential I will let it wait until I get one.
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liloldme
( ͝° ͜ʖ͡°)つ=D
Registered: 05/15/04
Posts: 5,085
Loc: Zone 8
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Skelsen]
#24695604 - 10/09/17 02:21 AM (3 years, 3 months ago) |
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It would be a wise investment if you're considering this hobby.
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bobdylan0423
Stranger
Registered: 01/10/16
Posts: 6
Last seen: 3 years, 2 months
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: liloldme]
#24695616 - 10/09/17 02:28 AM (3 years, 3 months ago) |
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ok say i have ~50 dollars and no PC,but i have cubensis spores on syringe and want to grow them and dry them for later (obviously). how would you guys go about that?
Thankyou
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Pastywhyte
Say hello to my little friend
Registered: 09/16/12
Posts: 36,621
Loc: Canada
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: bobdylan0423]
#24695624 - 10/09/17 02:34 AM (3 years, 3 months ago) |
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Quote:
bobdylan0423 said:
ok say i have ~50 dollars and no PC,but i have cubensis spores on syringe and want to grow them and dry them for later (obviously). how would you guys go about that?
Thankyou
Read this shit
https://www.shroomery.org/forums/showflat.php/Number/19296230
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capn_cap
yunglilbaby
Registered: 05/10/17
Posts: 6
Loc: OR 
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! *DELETED* [Re: Pastywhyte]
#24695867 - 10/09/17 06:01 AM (3 years, 3 months ago) |
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Post deleted by capn_capReason for deletion: used search
Edited by capn_cap (10/09/17 07:42 AM)
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Yonatin
Registered: 09/05/17
Posts: 597
Loc:
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: capn_cap]
#24696742 - 10/09/17 03:16 PM (3 years, 3 months ago) |
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Is this a magic mushroom?
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Mad Season
hookers and blackjack
Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Yonatin]
#24696747 - 10/09/17 03:17 PM (3 years, 3 months ago) |
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No.
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Yonatin
Registered: 09/05/17
Posts: 597
Loc:
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Mad Season]
#24696824 - 10/09/17 03:44 PM (3 years, 3 months ago) |
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Ok thanks
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KidMyco4296
Registered: 10/03/17
Posts: 89
Last seen: 6 months, 6 days
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Yonatin]
#24697032 - 10/09/17 04:40 PM (3 years, 3 months ago) |
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Is shaking your grains still necessary with cakes or anything with a casing? I have verm layers but I imagine shaking would ruin that.
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Mad Season
hookers and blackjack
Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KidMyco4296]
#24697077 - 10/09/17 04:57 PM (3 years, 3 months ago) |
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Shaking should pretty much only be done with grains. At the very least it needs to be done in an airtight container with a nice filter. If you have a dry verm layer as your filter, or have an open substrate with a casing, shaking is detrimental.
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potnug
Registered: 07/06/17
Posts: 321
Last seen: 1 year, 6 months
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Mad Season]
#24698874 - 10/10/17 09:20 AM (3 years, 3 months ago) |
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What are a few good ways to germinate and get a clean culture from a bacterial syringe that won't germinate on pda agar?
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stareatclouds
star eat clouds?
Registered: 09/29/14
Posts: 9,444
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: potnug]
#24700108 - 10/10/17 07:27 PM (3 years, 3 months ago) |
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I've always seen spore syringes made the following way: scrape spores into a shot glass, spray sterile water into it, and then aspirate/spray 3-5x before capping the syringe.
Assuming there's a reasonably clean print, including the foil casing, is there a much bigger risk for contamination if you simply dropped the foil into a jar of sterilized water for SHIP aspiration?
Obviously spores are inherently dirty and there's always risk in creating syringes from them, but with a properly cared for print, any reason not to try this? It just seems easier overall and can make more syringes. The only other issue I can think of is maybe the spores won't dislodge without scraping initially.
Follow up question: I doubt people making syringes for vendors are going 1 shot glass at a time. What's the common method for bulk syringe making?
Edited by stareatclouds (10/10/17 07:35 PM)
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