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OfflinePussyFart
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10 PE ISOLATES * 16
    #18219379 - 05/05/13 09:36 PM (9 years, 3 months ago)

So I took tissue samples from 3 nice MS PE fruits and placed them on agar.

   

After a few transfers, I had 10 plates that looked like this.

   

I then cut a nice wedge from each dish and inoculated 10 master grain jars with them.

Took about 2.5 weeks for full colonization of all jars, shaking each jar once at 20-30%.

Once they were all fully colonized, I expanded the mycellium via G2G(Grain to Grain) to 9 quarts each.

Grains were WBS(Wild Bird Seed) rinsed and soaked in hot tap water for 24 hours, then strained for 20 minutes, loaded into quart jars and PCed for 100 minutes at 15psi.

Once all 90 were fully colonized, 8 quarts from each batch/culture(1-10) were spawned to 1 brick of coir(650g) and 3 quarts of verm following Damion5050's Bucket Tek.

I used 5.5qts of water in my preparation. This equals out to 4qts for the brick of coir, and .5qts for each quart of verm.

They were spawned in monotubs and were all fully colonized in 10 days, and then fruited.(No consolidation)

Spawn was mixed evenly with the substrate with no top layer and have all shown just about equal colonizations speeds.




TUB - ISOLATE #1
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 8.5oz DRY)


TUB - ISOLATE #2 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT!
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22


TUB - ISOLATE #3
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                        (HARVEST 6oz DRY)


TUB - ISOLATE #4 - UNFORTUNATLEY LOST TO TRICH - :sad:
   
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11


TUB - ISOLATE #5 - NOTHING AFTER A MONTH - AINT NOBODY GOT TIME FOR THAT!
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22


TUB - ISOLATE #6
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 5.5oz DRY)


TUB - ISOLATE #7 - HIT WITH TRICH/COBWEB - ISOLATED TO FINISH FLUSH
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 23
                                                                                                                                      (HARVEST 5.75oz DRY)


TUB - ISOLATE #8 - HIT WITH TRICH AFTER 3 WEEKS - TOSSED
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                              FRUITING DAY 17

 
              FRUITING DAY 20                                FRUITING DAY 22


TUB - ISOLATE #9
       
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 26
                                                                                                                                      (HARVEST 7.3oz DRY)


TUB - ISOLATE #10
         
              FRUITING DAY 1                                  FRUITING DAY 8                                FRUITING DAY 11                                FRUITING DAY 15                    FRUITING DAY 17(HARVEST)

   
              FRUITING DAY 20                                FRUITING DAY 22                              FRUITING DAY 26
                                                                                                                                      (HARVEST 4.4oz DRY)



RANDOM PICS

   


First Flush Totals:

Blobs      4.00oz
Tub #1    8.50oz
Tub #3    6.00oz
Tub #6    5.50oz
Tub #7    5.75oz
Tub #9    7.30oz
Tub #10  4.40oz 
Total  =  41.45 Ounces of Cock!



Edited by PussyFart (05/17/13 04:48 PM)


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OfflinecronicrMFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 6 months, 17 days
Trusted Cultivator
Re: 10 PE ISOLATES [Re: PussyFart]
    #18219392 - 05/05/13 09:39 PM (9 years, 3 months ago)

:rockon::thumbup::jiggletits::gethigh::likeaboss::winner::chalkup::yourock::heytheresexy::picdidnthappen:
:excited::homerdrool:
see you at the finish line hacker, nice pics


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: 10 PE ISOLATES [Re: cronicr] * 1
    #18219401 - 05/05/13 09:41 PM (9 years, 3 months ago)

Hey this is awesome!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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Offlineroxxor
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Registered: 08/29/12
Posts: 251
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Re: 10 PE ISOLATES [Re: PussyFart]
    #18219410 - 05/05/13 09:43 PM (9 years, 3 months ago)

:popcorn:


--------------------


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OfflineRiparianZoneJunky
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Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 2 years, 9 days
Re: 10 PE ISOLATES [Re: Violet]
    #18219421 - 05/05/13 09:45 PM (9 years, 3 months ago)

:firstladyofapproval:
Nice work man, can't wait to see the end results.  There can be only one!


--------------------
RZJ's Tea Tek
RZJ's Tradelist


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OfflineiOmniphobia
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Registered: 03/29/13
Posts: 148
Last seen: 10 months, 25 days
Re: 10 PE ISOLATES [Re: roxxor]
    #18219440 - 05/05/13 09:48 PM (9 years, 3 months ago)

Well done sir. Really interesting to watch 10 isolates grow so differently.
At once. Be watching this one


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,419
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Re: 10 PE ISOLATES [Re: iOmniphobia]
    #18222236 - 05/06/13 02:39 PM (9 years, 3 months ago)

Thanks guys, I try.


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Invisibleshroom-a-zoom
Stranger in a strange land


Registered: 02/26/13
Posts: 414
Loc: Fungiland Flag
Re: 10 PE ISOLATES [Re: PussyFart]
    #18222292 - 05/06/13 02:53 PM (9 years, 3 months ago)

Awesome work hacker. :super:


--------------------
How it should & shouldn't look - NEW CULTIVATORS GUIDE

:imo:Lets get rid of the SWIM shit, This also goes for my dog, someones pet ferret, and any other way of saying you are not really you.
Its bullshit and we all know it. Man up and admit you are the SWIM.:2cents:


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/05/11
Posts: 10,573
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Re: 10 PE ISOLATES [Re: shroom-a-zoom]
    #18222363 - 05/06/13 03:11 PM (9 years, 3 months ago)

:bananadance:
:congrats:

I fruited similar isolates like this a while ago..24 different plates and only one made no balls on the first flush. Ah, the elusive "clean penis" if you will :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflinePussyFart
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Re: 10 PE ISOLATES [Re: FrankHorrigan]
    #18222372 - 05/06/13 03:14 PM (9 years, 3 months ago)

I can only hope Frank, really, this is what I am hoping for with 2, 5 and 8, as they look like they are about to explode.


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OfflinePrinceShroom
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Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
Last seen: 8 years, 3 months
Re: 10 PE ISOLATES [Re: PussyFart]
    #18222399 - 05/06/13 03:20 PM (9 years, 3 months ago)

:popcorn:
Like Frank said no matter what you always get mutant blobs first flush.  Awesome work I look forward to seeing this progress.
:congrats:


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineSillyputty67

Registered: 10/06/12
Posts: 2,239
Loc: Netherlands
Last seen: 7 years, 11 months
Re: 10 PE ISOLATES *DELETED* [Re: PrinceShroom]
    #18222450 - 05/06/13 03:32 PM (9 years, 3 months ago)

Post deleted by malicom

Reason for deletion: 1



--------------------
1) Everything I ever posted or say is a lie.


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OfflineBangkok
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Registered: 04/14/13
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Re: 10 PE ISOLATES [Re: shroom-a-zoom]
    #18222467 - 05/06/13 03:35 PM (9 years, 3 months ago)

Nice work, interested in your results


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OfflinePrinceShroom
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Registered: 04/01/13
Posts: 395
Loc: Aiel Waste
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Re: 10 PE ISOLATES [Re: Sillyputty67] * 1
    #18222488 - 05/06/13 03:39 PM (9 years, 3 months ago)

Quote:

malicom said:
This keeps me apprehensive about growing PE. I like my non blob aa+ first flush.



The blobs are awesome though.  They will have you :trippinballs:


--------------------
WAR IS PEACE
FREEDOM IS SLAVERY
IGNORANCE IS STRENGTH

Need help? Feel free to :pm: me.


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OfflineMoosehead905
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Registered: 05/30/12
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Re: 10 PE ISOLATES [Re: PrinceShroom]
    #18222802 - 05/06/13 04:46 PM (9 years, 3 months ago)

:manofapproval:

you make it look so easy.


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: 10 PE ISOLATES [Re: Moosehead905]
    #18223525 - 05/06/13 07:22 PM (9 years, 3 months ago)

My PE isolates don't blob at all… beautiful specimens for first and every flush.
I didn't get any that did


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
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Loc: Orbiting Earth
Last seen: 2 days, 9 hours
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Re: 10 PE ISOLATES [Re: Violet]
    #18223751 - 05/06/13 08:10 PM (9 years, 3 months ago)

Quote:

Violet said:
My PE isolates don't blob at all… beautiful specimens for first and every flush.
I didn't get any that did



What was the substrate?


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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: 10 PE ISOLATES [Re: PussyFart]
    #18223929 - 05/06/13 08:47 PM (9 years, 3 months ago)

These are the only decent photos I have taken, tho there have been many lovely specimens on others since as well:

I bet you can guess the substrate ;] (strait grass seed)


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (05/09/13 11:38 AM)


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OfflineTheCyndicate
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Registered: 10/16/11
Posts: 1,195
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Last seen: 4 years, 3 months
Re: 10 PE ISOLATES [Re: Violet]
    #18224037 - 05/06/13 09:14 PM (9 years, 3 months ago)

Nice work hacker!!!
Tub 10 looks good. Cant wait to see how the others are gonna do.

Cyn


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OfflineSLAP
Moon Shadow
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Registered: 04/26/11
Posts: 62
Last seen: 9 years, 28 days
Re: 10 PE ISOLATES [Re: Violet]
    #18224045 - 05/06/13 09:15 PM (9 years, 3 months ago)

Awesome post!

:popcorn:


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