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InvisibleMunchauzen
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Blenderless Liquid Inoculate * 27
    #22833314 - 01/28/16 10:02 AM (4 years, 7 months ago)

Blenderless Liquid Inoculate

you will need:
- mason jar with unmodified lid
- receiving jars with grains or bottle substrate
- approximately 25-50ml sterilized water per jar to inoculate
- clean culture on agar plate
- 70% isopropyl alcohol
- paper towels
- gloves
- SAB

after you have prepped your SAB and work space with all the things needed, proceed with the following:

Phase One 'the drop and shake'
- wipe down the water jar lid band with an iso paper towel
- remove the lid band and set it aside
- use your fingers or a sterilized tool to break the seal on the jar lid
- gently place the lid back on the jar
- wipe down your cup or petri with an iso paper towel
- squeeze the cup to loosen the agar puck from the cup, if you have petris use a tool to loosen it from the dish
- in one smooth motion, lift the water jar lid and culture lid, then drop the agar puck into the water jar
- immediately replace the lid band and tighten
- remove the water jar from the SAB and retighten
- shake the shit out of the water jar
- return the water jar to the SAB

Phase Two 'the pour'
- wipe down the water jar lid band with an iso paper towel
- remove the lid band and set it aside
- wipe down your receiving jar lid bands with an iso paper towel
- loosen all the lid bands and gently lift the lids and bands together, then set them back down on top of the jars
- swirl your water jar to evenly distribute the agar and mycelium in the water
- remove the water jar lid and set it aside
- in one smooth motion, lift one of the receiving jar lids and pour a splash of water in
- continue to swirl your water jar between receiving jars
- repeat last two steps until all receiving jars are inoculated
- put water jar lid and band back on, tighten the band
- tighten all receiving jar lids
- remove jars from the sab
- retighten all lid bands
- shake receiving jars until the agar is visibly evenly distrubited throughout the jar

FAQ
Q: what's the best agar recipe for this tek?
A: I recommend a softer recipe, check out my Agar as easy as 1-2-3! tek for my reccomended recipe.

Q: can I keep and use any leftovers?
A: if you follow good sterile technique, any leftovers should be fine to use. beginners best err on the side of caution. you can always pour leftovers into your final jar - it will colonize even faster than the rest!

Q: can I use a jar lid with a SHIP and make syringes?
A: absolutely!

Q: can I store LI?
A: While LI is intended to use immediately, it can be stored for several weeks in a refrigerator

Q: how long until I see growth?
A: typically, 2-4 days

Q: how long until 100% colonization?
A: 1.5-2 weeks

Q: do I shake at 30%?
A: ideally no, you should have shaken it well enough to evenly coat everything when you poured it, and that will result in even colonization. however, if you do end up with uneven growth, you can indeed shake at 30%.


Video


music: Phish - Ghost - Prague, Czech Republic 7/6/1998


RESULTS


ESS monotub
5 quarts oats
bucket tek cvg
jiffy casing
3 oz dry


Mexicana Jalisco monotub ~ 1 month growth
5 quarts of oats
bucket tek cvg
90 grams dry stones


MS Stropharia monotub
spawned 3/21 - harvested 4/10
5 quarts oats
bucket tek cvg
top layer of cvg (pseudo-case)
blenderless LI

2nd flush


MS Stropharia monotubs
2017
4 Quarts of oats per tub
Bucket tek'd coir x 9 in a large rubbermaid
Top layer of coir
Blenderless LI


Cloned AA+ shoeboxes
Unknown dates
1 Quart of oats per shoebox
1 Quart of coir per shoebox
Bucket tek'd coir x 4 in a large rubbermaid
No casing layer
Blenderless LI


Edited by Munchauzen (07/26/20 03:59 PM)


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OfflineSloppyJoseph
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Re: Blenderless Liquid Inoculate [Re: Munchauzen] * 1
    #22833320 - 01/28/16 10:03 AM (4 years, 7 months ago)

:rockon: hahah it's almost like I was waiting for this..


:leocheers:


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Offlinetripdawg420
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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833347 - 01/28/16 10:15 AM (4 years, 7 months ago)

:thumbup:


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http://www.shroomery.org/forums/showflat.php/Number/11917410
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Offlinemushpunx
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Re: Blenderless Liquid Inoculate [Re: SloppyJoseph] * 1
    #22833366 - 01/28/16 10:19 AM (4 years, 7 months ago)

So you're essentially shaking the wedge in sterile water to get the myc off yea?

Nice write up man cool video I like the split screen thing!

The other day I was looking to see if they make a magnetic stir bar with blades on it or modifying them to have blades. Maybe use a magnetic stir plate to blend LI. You could buy a few stir bars and do multiple LIs pretty easily


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InvisibleMunchauzen
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Re: Blenderless Liquid Inoculate [Re: mushpunx]
    #22833382 - 01/28/16 10:26 AM (4 years, 7 months ago)

well, the agar itself is colonized. so the most important thing is breaking up the agar. it has filamentous strands of mycelium running throughout that we cannot see with our eyes. thats why a chunk of agar with no visible exterior mycelium on it will begin to grow again once you inoculate with it. here is a pic of one of the jars thats in the video. notice that this chunk is regrowing from all directions. it does because the interior of the agar is full of mycelium.



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Offlinemushpunx
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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833392 - 01/28/16 10:30 AM (4 years, 7 months ago)

So your blender less tek actually breaks up the agar pretty well I didnt know shaking was enough


I sugguest you buy some of the one piece plastic autoclaveable , that way you dont have to fuck around with the lid under the band


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Edited by mushpunx (01/28/16 10:40 AM)


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InvisibleMunchauzen
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Re: Blenderless Liquid Inoculate [Re: mushpunx]
    #22833471 - 01/28/16 11:01 AM (4 years, 7 months ago)

yeah, having a one piece lid would be nice. good idea

Quote:

mushpunx said:
So your blender less tek actually breaks up the agar pretty well I didnt know shaking was enough




these were pretty stiff pucks too. I'd imagine you'd get even better results with a bit softer puck. I'd love to see how a cornmeal plate does on this.


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Invisible13shroomsM
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Re: Blenderless Liquid Inoculate [Re: Munchauzen] * 2
    #22833534 - 01/28/16 11:28 AM (4 years, 7 months ago)

:awesome2:

:smokeweedallday:


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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833583 - 01/28/16 11:47 AM (4 years, 7 months ago)

Great tek, this will make things soo much easier!

I wasn't too thrilled about having to mess with silicone and making one piece lids for my LI,
then having to blend them. Too much work.
I'll be trying this out soon with one of my fully colonized plates. :rockon:


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InvisibleStickyIcky Fingers
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Re: Blenderless Liquid Inoculate [Re: 13shrooms] * 2
    #22833586 - 01/28/16 11:48 AM (4 years, 7 months ago)

:rockon: I had been spraying plates with a syringe of sterile water and sucking it back up then inocing this looks much more effective


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OfflineMiscusi
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Re: Blenderless Liquid Inoculate [Re: 13shrooms]
    #22833589 - 01/28/16 11:48 AM (4 years, 7 months ago)

Quote:

13shrooms said:
:awesome2:

:smokeweedallday:





Guess who found the TEK  they are going to use :laugh:

<-- This guy.  Thanks Munch. I was going to go get the oster attachment n BS. But this is nice..


:borat: Verry niiice.


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InvisibleMunchauzen
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Re: Blenderless Liquid Inoculate [Re: StickyIcky Fingers]
    #22833630 - 01/28/16 12:04 PM (4 years, 7 months ago)

Quote:

StickyIcky Fingers said:
:rockon: I had been spraying plates with a syringe of sterile water and sucking it back up then inocing this looks much more effective




yup I did that for a while. I have a video of it somewhere, not sure if I ever published it. this gives the added benefit of using the agar puck, which is full of nearly invisible filamentous mycelium.


Edited by Munchauzen (01/28/16 12:05 PM)


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InvisibleMunchauzen
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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833643 - 01/28/16 12:07 PM (4 years, 7 months ago)

I have the oster attchment, and I'm still going to keep using it. This tek will just supplement it, so I can make more than one LI per work session. The blender will break it up a little bit better than the shaking, thus making more inoculation points. Both work great, though!


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InvisiblePastywhyteM
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Re: Blenderless Liquid Inoculate [Re: Miscusi] * 1
    #22833706 - 01/28/16 12:30 PM (4 years, 7 months ago)

Simple and easy. Great stuff munch :thumbup:


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OfflineSloppyJoseph
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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833754 - 01/28/16 12:42 PM (4 years, 7 months ago)

Quote:

Munchauzen said:
Quote:

StickyIcky Fingers said:
:rockon: I had been spraying plates with a syringe of sterile water and sucking it back up then inocing this looks much more effective




yup I did that for a while. I have a video of it somewhere, not sure if I ever published it. this gives the added benefit of using the agar puck, which is full of nearly invisible filamentous mycelium.




You did it's in with the videos in your sig.  :thumbup:

I ended up using that old tek for my bag inoculation, I had a syringe and didn't have a fully sterile jar. But I have about 7 more qts on deck and a few transfers I just did waiting for this one.


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Invisiblemaddchef
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Re: Blenderless Liquid Inoculate [Re: SloppyJoseph]
    #22833782 - 01/28/16 12:47 PM (4 years, 7 months ago)

Don't need a marble or anything to help agitate?


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Invisiblethe_r3dz
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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22833950 - 01/28/16 01:42 PM (4 years, 7 months ago)

Quote:

Munchauzen said:
yeah, having a one piece lid would be nice. good idea

Quote:

mushpunx said:
So your blender less tek actually breaks up the agar pretty well I didnt know shaking was enough




these were pretty stiff pucks too. I'd imagine you'd get even better results with a bit softer puck. I'd love to see how a cornmeal plate does on this.





Great, because that's literally what I'm doing right now.

Well, basically. I was going to put a piece of glass into the jar of water. I should have realized that having less water in the jar is a key factor though. My jar was full of water when I first tried this and the agar didn't break up at all...duh.
Made up some cornmeal plates last night. Eat says he doesn't think it will work but I'm going to try to convince him otherwise.


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Re: Blenderless Liquid Inoculate [Re: Munchauzen]
    #22834010 - 01/28/16 02:06 PM (4 years, 7 months ago)

Great stuff Munch! :rockon:

nice touch vid the video editing!


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Re: Blenderless Liquid Inoculate [Re: spacechildo]
    #22834031 - 01/28/16 02:11 PM (4 years, 7 months ago)

Nice write up man. I personally just use pastyplates till I have clean growth then inject said plate with sterile water, scratch surface with needle, then suck it back up.


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InvisibleMunchauzen
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Re: Blenderless Liquid Inoculate [Re: spirit_shadow] * 1
    #22834053 - 01/28/16 02:16 PM (4 years, 7 months ago)

Quote:

spirit_shadow said:
Nice write up man. I personally just use pastyplates till I have clean growth then inject said plate with sterile water, scratch surface with needle, then suck it back up.




thats another one of my teks... and I am replacing it with this tek. Reason being is you are leaving behind many many inoculation points doing so. the agar itself is full of mycelium. its not just on the surface.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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