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InvisibleButtFace
Registered: 08/06/08
Posts: 1,588
Re: Official Psilocybe zapotecorum thread *DELETED* [Re: the_chosen_one]
    #28199294 - 02/22/23 02:28 PM (1 year, 9 months ago)

Post deleted by ButtFace

Reason for deletion:

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OfflineGalavant
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Re: Official Psilocybe zapotecorum thread [Re: ButtFace] * 2
    #28199363 - 02/22/23 02:53 PM (1 year, 9 months ago)

I can DM u where I got mine if u want. I’ve done 3 orders and been happy so far.


--------------------
My humble grow journal

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***ANY and ALL*** posts by me should be considered satirical in nature and strictly for the lulz

Edited by Galavant (02/22/23 02:59 PM)

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Offlinescapo
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Re: Official Psilocybe zapotecorum thread [Re: the_chosen_one] * 1
    #28199762 - 02/22/23 07:34 PM (1 year, 9 months ago)

Ahh I see! Good to know yes it could be the genetics I'm working with


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Offlinemushroom_gnome
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Re: Official Psilocybe zapotecorum thread [Re: scapo] * 1
    #28200389 - 02/23/23 09:17 AM (1 year, 9 months ago)

Thanks for making this thread Hindsight.
This species is about to get popular.


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Mystery Mushroom

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InvisibleCommanderDemander
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Registered: 11/07/22
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Re: Official Psilocybe zapotecorum thread [Re: mushroom_gnome]
    #28206829 - 02/27/23 01:34 PM (1 year, 9 months ago)

Claiming a spot on this thread. Very interesting mushroom; hopefully my next step after a few more cube grows.

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Offlinescapo
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Re: Official Psilocybe zapotecorum thread [Re: CommanderDemander] * 2
    #28207370 - 02/27/23 08:56 PM (1 year, 9 months ago)

Just toss it in the mix with your cubes!


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InvisibleEgon_Spengler
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Re: Official Psilocybe zapotecorum thread [Re: scapo]
    #28207390 - 02/27/23 09:20 PM (1 year, 9 months ago)

:camping:


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InvisibleCommanderDemander
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Re: Official Psilocybe zapotecorum thread [Re: scapo]
    #28207715 - 02/28/23 06:17 AM (1 year, 9 months ago)

Quote:

scapo said:
Just toss it in the mix with your cubes!



That's the plan but I'm still fairly new so I don't have any zap spores yet and my technique is still lacking since 3 of 4 shoeboxes I've run so far turned bacterial.

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Offline0t0lerance
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Re: Official Psilocybe zapotecorum thread [Re: CommanderDemander] * 3
    #28207845 - 02/28/23 08:59 AM (1 year, 9 months ago)

Only noticed the thread now :smile: ! Nice hindsight! Unfortunately I'm still seeing abort after abort after abort.. Went from too dry to way too wet conditions, I think.. so I'm trying the shortest duration of 1 min each 1,5h as of today and spread that throughout the day to keep humidity up.

here the result with the humidifier on for 5 minutes in a row, each 1,5h (bubbler is on 24/7):

27-feb:

28-feb, 3 new aborts:

Edited by 0t0lerance (02/28/23 08:59 AM)

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Offlinescapo
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance] * 1
    #28210266 - 03/01/23 08:52 PM (1 year, 8 months ago)

Try watering with a syringe instead of misting them. I also got lots of aborts from misting but when I went hands off and only watered with a syringe the fruits survived


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Offline0t0lerance
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Re: Official Psilocybe zapotecorum thread [Re: scapo] * 1
    #28210447 - 03/01/23 11:47 PM (1 year, 8 months ago)

Quote:

scapo said:
Try watering with a syringe instead of misting them. I also got lots of aborts from misting but when I went hands off and only watered with a syringe the fruits survived




Already done that, see journal "zap mex - talpa#1". Casing is absolutely soaked. but I suspect the top mist from the humidifier landing on top of the fruits directly is causing the aborts.. even slowed down the bubbler to 50% to see if that makes a difference (less air movement). I have hundreds of pins all aborted and the new ones popping up are aborting eventually. so I suspect the indirect flow is they key thing I'm missing at the moment.. the graveyard:


So I'm reversing the airflow with this design.. probably I'll lower the rack a bit more

Edited by 0t0lerance (03/02/23 02:41 AM)

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OfflineHindsight
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance] * 1
    #28210660 - 03/02/23 07:25 AM (1 year, 8 months ago)

0t0, sorry to hear about your aborts. It seems you are doing as I have done - changing things up often in order to find something that works. On one hand, you have to do that in order to find something that works, but on the other hand, with this species, I have found that they prefer to have very consistent conditions. As high humidity as you can get with plenty of FAE, and never allowed to dry. If you can design something that will do this, and force yourself to leave them alone after achieving that, they seem to really thrive on their own.

My second container of Zaps is pinning now in my first test-bubbler (same culture and same bubbler I fruited my last container in), and I don't plan on misting it so it will be interesting to see if I get a solid flush without a bunch of aborts. The previous container had many aborts because I kept moving it from system to system and trying different things until the aborts stopped.

It's not on the topic of Zaps, but on the topic of fruiting chambers, my Paps got pretty big but ultimately aborted so will be trying the same approach with them: no misting.

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Offline0t0lerance
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Re: Official Psilocybe zapotecorum thread [Re: Hindsight] * 2
    #28210702 - 03/02/23 08:13 AM (1 year, 8 months ago)

Quote:

Hindsight said:
0t0, sorry to hear about your aborts. It seems you are doing as I have done - changing things up often in order to find something that works. On one hand, you have to do that in order to find something that works, but on the other hand, with this species, I have found that they prefer to have very consistent conditions. As high humidity as you can get with plenty of FAE, and never allowed to dry. If you can design something that will do this, and force yourself to leave them alone after achieving that, they seem to really thrive on their own.

My second container of Zaps is pinning now in my first test-bubbler (same culture and same bubbler I fruited my last container in), and I don't plan on misting it so it will be interesting to see if I get a solid flush without a bunch of aborts. The previous container had many aborts because I kept moving it from system to system and trying different things until the aborts stopped.

It's not on the topic of Zaps, but on the topic of fruiting chambers, my Paps got pretty big but ultimately aborted so will be trying the same approach with them: no misting.




Yes, I hope this will do the trick, probably the fact that the big fat cake is filling the entire FC and doesn't help in this respect, currently have another tub running with a cake half the size, time will tell. I'll continue these things in my journal and won't add it here unless I have some fruits to show for it, a well.. in my next post, I hope to show the same tub.. filled with fruits instead of aborts. Good luck with the Papuana! I'll keep an eye on this thread :eyeball:

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Offlinescapo
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance]
    #28210733 - 03/02/23 09:02 AM (1 year, 8 months ago)

I won’t lie things went way better the less I played around with it. I watered once a week keeping it wet and in regular cube conditions and they fruited and survived.


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InvisibleBaba Yaga
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Registered: 09/13/20
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance]
    #28210878 - 03/02/23 11:01 AM (1 year, 8 months ago)

Quote:

0t0lerance said:







What's the spot on the left?

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Offline0t0lerance
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Re: Official Psilocybe zapotecorum thread [Re: Baba Yaga]
    #28210954 - 03/02/23 11:48 AM (1 year, 8 months ago)

Quote:

Baba Yaga said:
Quote:

0t0lerance said:







What's the spot on the left?




Removed casing there as I had some mold solely on the casing (made the mistake by using peat humus). This was almost 2 weeks ago. Mold didn't come back.

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InvisibleBaba Yaga
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance]
    #28210964 - 03/02/23 11:56 AM (1 year, 8 months ago)

Unfortunately my sect zap spores are not germinating so I can't speak from first hand experience with this species but from my pan grows I have seen dense/clustering pin sets like yours a few times and they always had problems and aborted easily.

I would consider that contamination is stuffing this up instead of conditions and start over.

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OfflineGalavant
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Re: Official Psilocybe zapotecorum thread [Re: Baba Yaga]
    #28211013 - 03/02/23 12:38 PM (1 year, 8 months ago)

Quote:

Baba Yaga said:
Unfortunately my sect zap spores are not germinating so I can't speak from first hand experience with this species but from my pan grows I have seen dense/clustering pin sets like yours a few times and they always had problems and aborted easily.

I would consider that contamination is stuffing this up instead of conditions and start over.




I put some more Zap spores on fresh agar last night along with some Pollock’s Tamp and ATL7. I’ve had some Zap spores on agar for over a week and am worried they won’t pop. Hopefully it was just a bad batch of agar or something.......


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Offline0t0lerance
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Re: Official Psilocybe zapotecorum thread [Re: Baba Yaga] * 2
    #28211067 - 03/02/23 01:24 PM (1 year, 8 months ago)

Quote:

Baba Yaga said:
Unfortunately my sect zap spores are not germinating so I can't speak from first hand experience with this species but from my pan grows I have seen dense/clustering pin sets like yours a few times and they always had problems and aborted easily.

I would consider that contamination is stuffing this up instead of conditions and start over.




Well, I'm giving the big cake another shot just in case. Decided to remove the entire casing and case with Inski's recipe.. lowered the rack at the same time. I noticed dozens of new pins everywhere in between the casing layer upon removal.. many pins where really anchored in the cake, which made it difficult to remove. After the casing was cleared I checked for mold, but there wasn't any, as far as it's possible to say anything about that.. Also noticed tons of side-pins developing, I would say it indicated the cake is still ok-ish after 2,5 months (I think).

Unfortunately there is very little space between the cake and the walls of the tub. But we'll see what will happen, hope enough fresh air spreads around the surface, I'll just cross my fingers and wait. Regarding germination, all spores germinated fine on water agar. Plain lmea worked ok too, but the mycelium spreads faster on water agar so that is my go-to for germination. Out of 14 plates, 13 ended up clean going to T1, however,.. with Zap 'La Martinica' I do notice these yellow patches on T1 I haven't seen before (3 wedges out of 48).. looks different from what I normally would consider bacterial, seems like the mycelium itself has a yellow hue. anyone an idea?

Edited by 0t0lerance (03/02/23 01:38 PM)

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Offlinescapo
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Re: Official Psilocybe zapotecorum thread [Re: 0t0lerance]
    #28211086 - 03/02/23 01:39 PM (1 year, 8 months ago)

What’s Inski’s recipe?

Also from this observation, would you say it’s possible that the casing layer may not be promoting pins? That they’ll pin from the substrate alone given the right moisture and FAE?


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Updated as of 11/10/2024

https://www.inaturalist.org/people/scapo9688

Edited by scapo (03/02/23 01:39 PM)

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