mushboy said:

Got pics of the culture you use to inoculate the broth?

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Appreciate the help guys. It was from a Plate Pin off an overgrown dish I had already taken 2 transfers from which sounds ridiculous now that I'm typing it lol. It had no signs of contam that I could see but I'm a meat head so. Bad idea?

I'm going to be making a new LC today with a T2 dish I have. Here are images of both. Do you think this one's more suitable?

Sericin said:

Vendor name removed; thank you for that note.

I did test on agar. Because oats are cheap and I have a fifty-pound bag of them, and I was excited to get started, I did both LC to agar (to test) and LC to grain at the same time - I figured if there was an issue, I was out like a buck and a half worth of oats and half an hour. My agar plates were pretty clean: evenly round growth with no contamination I could see within the mycelium. I did six plates for each syringe, mainly so I could watch them grow and see how they behave. One I dropped some ISO on by accident and the growth was off-round, and a couple of them did get some not-in-the-mycelium contamination (one mold, two bacterial, out of 20 plates) that was introduced during inoculation of the plates... but the culture looked pretty clean. I don't think there was bacteria in the syringe, although I can't swear that my technique was perfect. There's definitely a learning curve! Even though the T0 plates from the LC looked pretty clean, I did T1 transfers just to practice (and I was also cleaning up / selecting for growth potential some T0 plates from spore print at the same time), and I put a couple of the nice clean T1 plates of agar to grain, and they're doing well.



What diagnoses it as bacterial? I'm new to mushrooms, but familiar with biology lab - I know what I'm looking at on an agar plate, but not in a jar of oats. The white clumps look to me like dense mycelium; they don't have the weepy/rotted look that I always associate with bacterial growth. Close to, there is still fuzzy mycelium covering every oat, it's just not solid white like I want it to be (and like the APE jars that I did at the same time were). When I've seen bacterial jars on here and other forums, they looked gross, like the jar was full of ick - this just looks like it was suspended in time, like it's going nowhere. I was beginning to wonder if somehow I had shaken it too much, but I shook the successful jars very similarly, and they bounced back just fine.

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BrokenHeart said:

Myce or mold.

Thinking it's mold and I need to start completely over,  Took pins from stalled cake that was getting contaminated wash/rinse with distilled water 4 times.  Shown from underneath, very airal. 72 hours 75deg.

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I see some good myc, but I also see sussy wispy myc. If you can grab some of that dense white growth, do that

Donniemarko said:

I don't get how to write a post without answering someone. I would like to know if I can get spores from a growing harvest to make a new one. I'm sure it's possible but I don't know how nor I can found info online

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At the top of the forum there's a "New Post" button, but we have a thread for questions like this:

https://www.shroomery.org/forums/showflat.php/Number/27827195

As for making spore prints:

https://www.shroomery.org/forums/showflat.php/Number/24461957

I would also encourage the use of the search engine:

https://www.shroomery.org/forums/showflat.php/Number/24270830

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