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InvisiblePickle Rick
Gherkin
I'm a teapot User Gallery


Registered: 07/24/22
Posts: 864
Loc: Jar
Re: Cultivation General Discussion [Re: nektar61] * 2
    #28297095 - 04/27/23 01:10 AM (8 months, 30 days ago)

interesting.. so far the pins I've had have just been miniature mature fruits :amanita2:


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P i c k l e    R i c k :dancer:



Pick Rick's Mushroom Chocolate


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Offlinenektar61S
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Registered: 07/04/20
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Re: Cultivation General Discussion [Re: Pickle Rick] * 3
    #28297105 - 04/27/23 01:38 AM (8 months, 30 days ago)

Quote:

Pickle Rick said:
interesting.. so far the pins I've had have just been miniature mature fruits :amanita2:



Some of the headless pins that grew into normal shrooms are in  these photos (along with some normal pins). All are "Z-Strain" variety, but I've also seen it with x7x. Top and bottom are the same area in same shoebox, but have different focus and show a little bit different view.

--


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-NEW? Start here.


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InvisiblePickle Rick
Gherkin
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Registered: 07/24/22
Posts: 864
Loc: Jar
Re: Cultivation General Discussion [Re: nektar61] * 2
    #28297172 - 04/27/23 04:56 AM (8 months, 29 days ago)

thought I'd share my recent "experiment"

two tubs, same batch of sub, same batch of lc. one had casing, the other had a few colonized grains left over in the bag sprinkled on top of the casing.

the one with the casing layer started fruiting before it was completely colonized even though the lid was on (I know fruiting conditions are set right away but I wanted to make sure everything was colonized first), so I flipped the lid when first pins appeared. the fruits are quite small and thin. I already harvested a few that were tearing their veils.



the second tub (the one with grainspawn on top) was kept closed until fully colonized, then I flipped the lid. its been fruiting slower, but the fruits are larger.



there are many pins on both... looking forward to some nice flushes.

On my next grow I think I'll just mix everything with no casing

... they are all leaning towards the light, I have to turn the tubs every now end then or they all go off to the same side


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P i c k l e    R i c k :dancer:



Pick Rick's Mushroom Chocolate


Edited by Pickle Rick (04/27/23 05:03 AM)


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OfflineKunta Kente
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Re: Cultivation General Discussion [Re: Pickle Rick] * 1
    #28297991 - 04/27/23 07:10 PM (8 months, 29 days ago)

In terms of what you guys prefer, if you get a fresh MSS, do you
1) agar it out then do several transfers of healthy mycelium to isolate one or two of the best myc
2) you just get a clean MSS agar plate and transfer a big chunk of that to grain and clone the best grower.

I have 3 MSS varieties i plated, transfered the best areas twice, and now have at LC stage for the best myc plates of each variety. Each final transfer plate looks quite uniform. I was reading up on more of bod's flowchart and extended info and glanced at a statement that sometimes (not infrequently?) isolated myc can grow great on a dish but still be a trash fruiter or just never fruit. Hence the  question for 1 or 2.


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Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.


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OfflineNonagon
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Registered: 09/01/22
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Re: Cultivation General Discussion [Re: Kunta Kente] * 1
    #28298016 - 04/27/23 07:40 PM (8 months, 29 days ago)

It’s hard to tell which plates will perform better without fruiting many of them. In my limited experience so far - aggressive/uniform growth on agar has generally carried over to more desirable growth on grain/bulk, and ultimately fruiting.

I’m sure it’s possible to have nice aggressive/uniform plate turn out bunk but I’ve yet to see it. I lack both the time and patience to commit to fruiting the less promising ones just in case :lol:


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ… πŸ…„ πŸ„½ πŸ„² πŸ„· πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineSan Pedro GirlS
Shoebox NinjaπŸ₯·
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Re: Cultivation General Discussion [Re: Kunta Kente] * 3
    #28298021 - 04/27/23 07:42 PM (8 months, 29 days ago)

Quote:

Kunta Kente said:
In terms of what you guys prefer, if you get a fresh MSS, do you
1) agar it out then do several transfers of healthy mycelium to isolate one or two of the best myc
2) you just get a clean MSS agar plate and transfer a big chunk of that to grain and clone the best grower.

I have 3 MSS varieties i plated, transfered the best areas twice, and now have at LC stage for the best myc plates of each variety. Each final transfer plate looks quite uniform. I was reading up on more of bod's flowchart and extended info and glanced at a statement that sometimes (not infrequently?) isolated myc can grow great on a dish but still be a trash fruiter or just never fruit. Hence the  question for 1 or 2.




I germinate a spore print on a bunch of plates. Then, I measure and transfer the largest/fastest growing germinations. I let those plates pin and send the pins to grain. The best fruits grown from that grain get printed and I start all over.


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OfflineA.k.aM
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Re: Cultivation General Discussion [Re: San Pedro Girl] * 8
    #28298029 - 04/27/23 07:47 PM (8 months, 29 days ago)

I prefer going to grain asap, I’ll use the actual germ plate if it’s clean but typically I use t1 or t2 and only go past that if it’s absolutely necessary to clean the culture.


You’ll see more variety this way so it depends what you want to do. I like to see as many phenos as possible to pick from.


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LAGM2020


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OfflineKunta Kente
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Re: Cultivation General Discussion [Re: San Pedro Girl] * 1
    #28298094 - 04/27/23 08:34 PM (8 months, 29 days ago)

Ok sweet, green light. Hopefully all the agar work pays off!


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Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.


Edited by Kunta Kente (04/27/23 08:36 PM)


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OfflineTyson
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Registered: 04/29/23
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Last seen: 7 months, 30 days
Re: Cultivation General Discussion [Re: Inocuole] * 2
    #28300391 - 04/29/23 08:59 PM (8 months, 27 days ago)

Feel free to roast haha first time cultivating and I’ve just thrown some horse sh*t in a plastic container with Panaeolus cyanescens and Psilocybe cubensis stems from a wild harvest can’t find much info on growing on sh*t so I’m hopefully asking you guys for any info if possible


Edited by Tyson (04/29/23 09:15 PM)


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Invisiblestubb
Dahg Rastubfari
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Registered: 03/23/19
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Re: Cultivation General Discussion [Re: Tyson] * 1
    #28300407 - 04/29/23 09:08 PM (8 months, 27 days ago)

Pigs and humans shit, moocows and horseys poo. 
Plug 'horse poo' into the search bar, much info to be had.

Also, welcome to the Shroomery!


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:mushroomgrow:
πŸ†ƒπŸ„΄πŸ…°πŸ„Ό  πŸ…²πŸ„»πŸ…ΈπŸ„½πŸ…ΆπŸ…†πŸ†πŸ„°πŸ…Ώ

You wake up. The room is spinning very gently round your head. Or at least it would be if you could see it which you can't.
It is pitch black.

> TURN ON LIGHT


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OfflineTyson
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Registered: 04/29/23
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Re: Cultivation General Discussion [Re: stubb]
    #28300409 - 04/29/23 09:11 PM (8 months, 27 days ago)

that’s what I got happening atm


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OfflineTyson
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Re: Cultivation General Discussion [Re: stubb] * 1
    #28300410 - 04/29/23 09:14 PM (8 months, 27 days ago)



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Offlinenektar61S
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Re: Cultivation General Discussion [Re: stubb] * 1
    #28300479 - 04/29/23 10:23 PM (8 months, 27 days ago)

Quote:

stubb said:
Pigs and humans shit, moocows and horseys poo. 
Plug 'horse poo' into the search bar, much info to be had.

Also, welcome to the Shroomery!





Also search
hpoo
It's often called that here.


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OfflineSirPsycho
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Re: Cultivation General Discussion [Re: Tyson] * 5
    #28300653 - 04/30/23 04:17 AM (8 months, 26 days ago)

Quote:

Tyson said:




Soo you just put shit in a tub and are hoping mushrooms grow... Cool.

All you'll get out of this is shit in a tub.


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:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


Edited by SirPsycho (04/30/23 06:35 AM)


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OfflineThrowaway
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Re: Cultivation General Discussion [Re: Tyson] * 3
    #28300655 - 04/30/23 04:26 AM (8 months, 26 days ago)

Quote:

Tyson said:




I can smell this picture


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Offlinefiddle_headS
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Re: Cultivation General Discussion [Re: Tyson] * 2
    #28300697 - 04/30/23 06:08 AM (8 months, 26 days ago)

Quote:

Tyson said:





I'll tell you, favorable responses to this method are going to be few and far between. A good place to start is with cubensis and pf tek; you can learn agar in conjunction to accelerate the process. But tossing a bunch of feces and grass and spores in an old tree trunk or pickle bucket or what have you a full canopy does not = good sir.
good day


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OfflineCapitan_Pacheco
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Registered: 02/15/23
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Re: Cultivation General Discussion [Re: Tyson] * 2
    #28300799 - 04/30/23 08:04 AM (8 months, 26 days ago)



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OfflineKunta Kente
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Re: Cultivation General Discussion [Re: Capitan_Pacheco]
    #28301780 - 05/01/23 04:01 AM (8 months, 25 days ago)

Curious, for each of your MSS...how many plates do you guys expand to. Like..MSS > you pick 2-3ish good areas, transfer. Then transfer 2-3 times to clean up and fully isolate. Then attempt to grow out? Or do you guys like to go MSS, pick a large clean sector (thereby being multiple different strains), go straight to a grow, clone 2-3 of the good ones as different genetic strengths will appear, go from there?

Trying to get an idea of popular methodology.


--------------------

Everything I have typed is purely fiction, and is purely for personal amusement.
All images I've posted are not mine and I am simply posting interesting things I found on the Google.


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
Last seen: 2 months, 25 days
Re: Cultivation General Discussion [Re: Kunta Kente]
    #28301790 - 05/01/23 04:17 AM (8 months, 25 days ago)

Anyone have experience with 150-200L autoclaves?

Looking at these, not sure if there's a cheaper unbranded alternative to be found on alibaba that anyone has had success with...

https://www.lorderan.com/products/150l-vertical-sterilizer

My AA 75x's keep breaking and they're not selling parts anymore last I spoke to WAFCO :thumbdown:


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
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Re: Cultivation General Discussion [Re: Mr. Mushie] * 1
    #28301857 - 05/01/23 06:25 AM (8 months, 25 days ago)

whats breaking on the 75x? them bitches are tanks you really gotta fuck with an AA to break them.


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