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Offlinecozmyc
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Re: Cultivation General Discussion [Re: Sericin] * 1
    #28244526 - 03/24/23 11:32 AM (10 months, 1 day ago)

Welcome :mushroom2: Yea, things got bacterial. You can toss them in a mix of straw and compost to possibly get fruits outside. It could have been leftover bacteria from sterilization cycle or from the LC. Always double check LC on agar first. Remove vendor name from your post please :mushroom2:


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OfflineSericin
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Re: Cultivation General Discussion [Re: cozmyc]
    #28244589 - 03/24/23 12:09 PM (10 months, 1 day ago)

Vendor name removed; thank you for that note.

I did test on agar. Because oats are cheap and I have a fifty-pound bag of them, and I was excited to get started, I did both LC to agar (to test) and LC to grain at the same time - I figured if there was an issue, I was out like a buck and a half worth of oats and half an hour. My agar plates were pretty clean: evenly round growth with no contamination I could see within the mycelium. I did six plates for each syringe, mainly so I could watch them grow and see how they behave. One I dropped some ISO on by accident and the growth was off-round, and a couple of them did get some not-in-the-mycelium contamination (one mold, two bacterial, out of 20 plates) that was introduced during inoculation of the plates... but the culture looked pretty clean. I don't think there was bacteria in the syringe, although I can't swear that my technique was perfect. There's definitely a learning curve! Even though the T0 plates from the LC looked pretty clean, I did T1 transfers just to practice (and I was also cleaning up / selecting for growth potential some T0 plates from spore print at the same time), and I put a couple of the nice clean T1 plates of agar to grain, and they're doing well.



What diagnoses it as bacterial? I'm new to mushrooms, but familiar with biology lab - I know what I'm looking at on an agar plate, but not in a jar of oats. The white clumps look to me like dense mycelium; they don't have the weepy/rotted look that I always associate with bacterial growth. Close to, there is still fuzzy mycelium covering every oat, it's just not solid white like I want it to be (and like the APE jars that I did at the same time were). When I've seen bacterial jars on here and other forums, they looked gross, like the jar was full of ick - this just looks like it was suspended in time, like it's going nowhere. I was beginning to wonder if somehow I had shaken it too much, but I shook the successful jars very similarly, and they bounced back just fine.


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OfflineBrokenHeart
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Re: Cultivation General Discussion [Re: Sericin]
    #28244604 - 03/24/23 12:19 PM (10 months, 1 day ago)

Myce or mold.

Thinking it's mold and I need to start completely over,  Took pins from stalled cake that was getting contaminated wash/rinse with distilled water 4 times.  Shown from underneath, very airal. 72 hours 75deg.


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OfflineBrokenHeart
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Re: Cultivation General Discussion [Re: BrokenHeart]
    #28244613 - 03/24/23 12:23 PM (10 months, 1 day ago)



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Offlinecozmyc
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Re: Cultivation General Discussion [Re: Sericin]
    #28244899 - 03/24/23 03:32 PM (10 months, 1 day ago)

Quote:

Sericin said:
Vendor name removed; thank you for that note.

I did test on agar. Because oats are cheap and I have a fifty-pound bag of them, and I was excited to get started, I did both LC to agar (to test) and LC to grain at the same time - I figured if there was an issue, I was out like a buck and a half worth of oats and half an hour. My agar plates were pretty clean: evenly round growth with no contamination I could see within the mycelium. I did six plates for each syringe, mainly so I could watch them grow and see how they behave. One I dropped some ISO on by accident and the growth was off-round, and a couple of them did get some not-in-the-mycelium contamination (one mold, two bacterial, out of 20 plates) that was introduced during inoculation of the plates... but the culture looked pretty clean. I don't think there was bacteria in the syringe, although I can't swear that my technique was perfect. There's definitely a learning curve! Even though the T0 plates from the LC looked pretty clean, I did T1 transfers just to practice (and I was also cleaning up / selecting for growth potential some T0 plates from spore print at the same time), and I put a couple of the nice clean T1 plates of agar to grain, and they're doing well.



What diagnoses it as bacterial? I'm new to mushrooms, but familiar with biology lab - I know what I'm looking at on an agar plate, but not in a jar of oats. The white clumps look to me like dense mycelium; they don't have the weepy/rotted look that I always associate with bacterial growth. Close to, there is still fuzzy mycelium covering every oat, it's just not solid white like I want it to be (and like the APE jars that I did at the same time were). When I've seen bacterial jars on here and other forums, they looked gross, like the jar was full of ick - this just looks like it was suspended in time, like it's going nowhere. I was beginning to wonder if somehow I had shaken it too much, but I shook the successful jars very similarly, and they bounced back just fine.



The stalling and patchy growth is what sticks out to me. Let them do their thing and see if any weird colors pop up, if not and they do fully colonize, you might be able to send them 1:1.

That T1 looks lovely


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You're conscious population 2 stardust
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and that's valuable :sunny:


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Offlinecozmyc
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Registered: 06/20/21
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Re: Cultivation General Discussion [Re: BrokenHeart] * 1
    #28244904 - 03/24/23 03:35 PM (10 months, 1 day ago)

Quote:

BrokenHeart said:
Myce or mold.

Thinking it's mold and I need to start completely over,  Took pins from stalled cake that was getting contaminated wash/rinse with distilled water 4 times.  Shown from underneath, very airal. 72 hours 75deg.




I see some good myc, but I also see sussy wispy myc. If you can grab some of that dense white growth, do that:thumbup:


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You're conscious population 2 stardust
----------------------
and that's valuable :sunny:


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OfflineBrokenHeart
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Re: Cultivation General Discussion [Re: cozmyc] * 1
    #28245052 - 03/24/23 05:07 PM (10 months, 1 day ago)

Not sure if I can save it because "the dense" formed around donor material and "the wispy" spread from it.  Serious head scratchin'  trying to keep genes going but doesn't appear to be going well.  I got one dish out of 8 that I sliced a myc chunk off side of cake instead of a stalled washed pin.  It doesn't appear to be sending out the wispy stuff yet and appears to be 10% larger, keeping fingers crossed.

Thank goodness for this site and you and your friends, I be mold farming repeatedly if it weren't for your kindness.  Seriously THANK YOU.


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Offlinecozmyc
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Re: Cultivation General Discussion [Re: BrokenHeart]
    #28245127 - 03/24/23 06:25 PM (10 months, 1 day ago)

No problemo :mushroom2:
If you still have that cake, maybe whip up some water agar and throw a chunk on it and see what happens. You might be able to get some separation.


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You're conscious population 2 stardust
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OfflineDonniemarko
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Re: Cultivation General Discussion [Re: Inocuole]
    #28245525 - 03/25/23 01:20 AM (10 months, 20 hours ago)

I don't get how to write a post without answering someone. I would like to know if I can get spores from a growing harvest to make a new one. I'm sure it's possible but I don't know how nor I can found info online


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Invisibledowodenum
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Re: Cultivation General Discussion [Re: Donniemarko] * 4
    #28245527 - 03/25/23 01:24 AM (10 months, 20 hours ago)

Quote:

Donniemarko said:
I don't get how to write a post without answering someone. I would like to know if I can get spores from a growing harvest to make a new one. I'm sure it's possible but I don't know how nor I can found info online




At the top of the forum there's a "New Post" button, but we have a thread for questions like this:

https://www.shroomery.org/forums/showflat.php/Number/27827195

As for making spore prints:

https://www.shroomery.org/forums/showflat.php/Number/24461957

I would also encourage the use of the search engine:

https://www.shroomery.org/forums/showflat.php/Number/24270830

Welcome to Shroomery :cookiemonster:


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OfflineHuskies
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Re: Cultivation General Discussion [Re: dowodenum]
    #28247393 - 03/26/23 11:07 AM (9 months, 30 days ago)

Eyyooo got some questions for y'all

1- Prepped some WBS, only one accessible had peanuts in it. I did my best to remove any, but some split ones might have avoided my keen eye. Will peanuts for sure contam, or is it likely that healthy myc can take care of it? I've used this before way back, but I don't remember if my tubs were iffy.

2- This birdseed has a bunch of wheat berries in it, and the prep I did yielded 13 jars. Can I store the extra jars in the fridge overnight if I did another run tomorrow? Or do I just toss them?

3- How much honey/karo are you using in your pastyplates? A drop, or more? My current plates seem slow, but it could be the new genetics (albino cambodian).

4- Another thing that might've been holding me back is not having cheap easy access to 100% potato flakes (I miss Idaho Spuds in the USA). The ones I have been using have like salt, some herbs and other ingredients.

It works, I've made great plates, but I wonder if they could've lead to higher contam rates than I expected.

5- Can I make pastyplates with Potato Starch instead?

Thanks in advance!


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OfflinedrVetker
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Re: Cultivation General Discussion [Re: Huskies]
    #28248155 - 03/26/23 06:48 PM (9 months, 30 days ago)

Hi everyone,

First time going to grain. Using unmodified lids.
Have 3 questions.

1.
Do I crack them open with a 1/4 turn immediately or do I wait until, e.g. first shake or something like that?

2.
Should I keep the bottles in some kind of a container, like a styrofoam box with a lid, or can I keep them in the cupboard?

3.
When do I shake? I'm looking at Mckenna's book (I know, outdated), he says on 4th, 6th, 8th and 10th day after inoculation.


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Invisiblemyc_ousin_vinny
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Re: Cultivation General Discussion [Re: drVetker] * 1
    #28248167 - 03/26/23 06:57 PM (9 months, 30 days ago)

Crack them a QUARTER INCH after inoculation. Not a quarter turn.

The cupboard is perfect.

I shake at 20% and 80% usually. It’s not based on time but percentage of colonization.


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Let's stay focused on what works consistently.

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Edited by myc_ousin_vinny (03/26/23 06:58 PM)


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Invisibledowodenum
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Re: Cultivation General Discussion [Re: myc_ousin_vinny]
    #28248183 - 03/26/23 07:10 PM (9 months, 30 days ago)

During PC and after inoculation they should be cracked. Between PC and inoculation, keep it fully tightened.

I'd recommend polyfil over unmodded lids though, surely you can borrow some stuffing from a pillow? :awesome:

You can keep them anywhere between 68-78ish Fahrenheit. Indirect sunlight is great, direct should be avoided, total darkness is meh.

Shake once at 20-30% colonization is consensus I think, don't shake on a schedule. You can also shake to test for contamination levels. Should see good recovery at least within a few days.


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Edited by dowodenum (03/26/23 07:16 PM)


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OfflinedrVetker
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Re: Cultivation General Discussion [Re: myc_ousin_vinny]
    #28248191 - 03/26/23 07:14 PM (9 months, 30 days ago)

Quote:

myc_ousin_vinny said:
Crack them a QUARTER INCH after inoculation. Not a quarter turn.

The cupboard is perfect.

I shake at 20% and 80% usually. It’s not based on time but percentage of colonization.




Ah fuck, I read pasty's lid tek and read it like 1/4 turn.
Thank you.


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OfflinedrVetker
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Re: Cultivation General Discussion [Re: dowodenum]
    #28248195 - 03/26/23 07:16 PM (9 months, 30 days ago)

Thank you sir


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Invisiblemyc_ousin_vinny
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Re: Cultivation General Discussion [Re: drVetker]
    #28248198 - 03/26/23 07:19 PM (9 months, 30 days ago)

Quote:

drVetker said:
Quote:

myc_ousin_vinny said:
Crack them a QUARTER INCH after inoculation. Not a quarter turn.

The cupboard is perfect.

I shake at 20% and 80% usually. It’s not based on time but percentage of colonization.




Ah fuck, I read pasty's lid tek and read it like 1/4 turn.
Thank you.




I made the same mistake when I first read it too.. Also, what dowodenum said about before, during and after etc is very important.


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OfflinedrVetker
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Re: Cultivation General Discussion [Re: myc_ousin_vinny]
    #28248201 - 03/26/23 07:20 PM (9 months, 30 days ago)

Oki doke. Thank you both


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Offlinepolaritymind
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Re: Why are these jars stalled? [Re: drVetker]
    #28249040 - 03/27/23 10:02 AM (9 months, 29 days ago)

Ive been having issues getting my tub to fruit, and suspect its due to surface conditions. It definitely doesnt have smalll water droplets on the surface like it says its supposed to in some. With my radiator on the air is quite dry (30%) and even misting twice a day seems to make the surface more wet than put small droplets on it. The mycelium also looks flat  and dull, not fluffy.
I used half a coir brick and 4 grain jars (720ml). I was wondering whether going back to alao using vermiculite, in the sense of cvg bulk material or also a pure verm casing might help, what do you think?
I can add pics to if neccesary. Oh and its been a month.


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InvisibleWay
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Re: Why are these jars stalled? [Re: polaritymind] * 1
    #28249160 - 03/27/23 11:37 AM (9 months, 29 days ago)

Quote:

polaritymind said:
Ive been having issues getting my tub to fruit, and suspect its due to surface conditions. It definitely doesnt have smalll water droplets on the surface like it says its supposed to in some. With my radiator on the air is quite dry (30%) and even misting twice a day seems to make the surface more wet than put small droplets on it. The mycelium also looks flat  and dull, not fluffy.
I used half a coir brick and 4 grain jars (720ml). I was wondering whether going back to alao using vermiculite, in the sense of cvg bulk material or also a pure verm casing might help, what do you think?
I can add pics to if neccesary. Oh and its been a month.




Sounds like misting and drying out created a matted surface that is now having a hard time absorbing water.

Straight coir is loved by many, including me, but I do think coir and verm is better. It definitely holds the water better and doesn't dry out as fast in my experience.  I wouldn't bother adding gypsum into the mix.

If you did coir and verm next time, I'm betting you'd end up misting less and have an easier time maintaining surface conditions.

For now, reduce fae and try to avoid misting as much. The mycelium will probably become fluffy again but who knows if it'll still fruit after this long.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


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