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 Nichrome
Virus Factory
 
Registered: 12/17/18
Posts: 7,773
Loc: Zone 5
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 Ask Quick Questions, Get Quick Answers   101
#27827195 - 06/19/22 11:48 AM (2 years, 9 months ago) |
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This is a place to ask quick questions. They happen all the time and this is a dedicated place.
Try to keep questions brief yet include as much detail as possible and try to keep answers brief and to the point.
Please do not refer people to the search function in this thread. Questions and answers only. Suggestions of search terms are fine and encouraged as well as instruction on how to use the search function and search function tact.
This is not a place to harass, demean, put down, or argue. People are allowed to disagree and that should not be misconstrued as an opening for argumentation or as a personal challenge. All views no mater how controversial are welcome to be expressed here regardless of post count, status, ratings, etc. Everyone has their own views and experiences and observations. All of it is valid in some way.
Good luck, have fun, and stay mushy.
~Shanti Sena~
Quick question,
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
Edited by fahtster (07/21/23 06:14 AM)
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 Rotnpins
🤮 Rotten-Pins 🍄
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 Re: Ask Quick Questions Here [Re: Nichrome]
#27827227 - 06/19/22 12:15 PM (2 years, 9 months ago) |
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 awesome!
Do you seal your swabs up right away or are you supposed to let them dry?
Quote:
Nichrome said:
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
Edited by Rotnpins (06/19/22 04:09 PM)
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Healing Oakland
Mushroom Co-op
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Re: Ask Quick Questions Here [Re: Nichrome] 1
#27827322 - 06/19/22 02:04 PM (2 years, 9 months ago) |
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Quote:
Nichrome said:
Quick question,
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
Quick answer:
Not necessary in my opinion/in my experience. WBS was the first grain I used back in the day and I removed the sunflower seeds as that was what some tek said they did, but in the end it isn't necessary, and having the varied grain size and nutrient profile doesn't hurt anything, and it's arguable that it helps in fact. Plus, it's less work.
Sweet thread! 
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san pedro guy
Captain
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Re: Ask Quick Questions Here [Re: Rotnpins] 1
#27827449 - 06/19/22 03:44 PM (2 years, 9 months ago) |
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Quote:
Nichrome said:
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
I take out sunflower seeds like I take out the yellow bits in capt crunch
Quick question:
If I drop a plate pin straight to grains, will it mess up the genetics if the cap opens or does it not matter because there are already spores in the cap by the time it forms?
thanks
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cozmyc
gentle modern ape
Registered: 06/20/21
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Re: Ask Quick Questions Here [Re: Nichrome]
#27827561 - 06/19/22 04:55 PM (2 years, 9 months ago) |
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 If I suspect I have grain jars that didn't quite sterilize correctly, can I run them through again?
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Healing Oakland
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Re: Ask Quick Questions Here [Re: cozmyc] 1
#27827728 - 06/19/22 07:17 PM (2 years, 9 months ago) |
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Quote:
cozmyc said:
Quick Question:
If I suspect I have grain jars that didn't quite sterilize correctly, can I run them through again?
Quick answer:
Yes you can PC them again. Done it more times than I care to admit lol.
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fahtster
Now With 33%More Faht
Registered: 06/17/06
Posts: 10,046
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Re: Ask Quick Questions Here [Re: Rotnpins] 4
#27827753 - 06/19/22 07:37 PM (2 years, 9 months ago) |
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Quote:
Rotnpins said:
awesome!
Do you seal your swabs up right away or are you supposed to let them dry?
Quote:
Nichrome said:
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
I pop em back in immediately and seal with tape. I do only swab one side of the tip tho so maybe that plays a role but I have 0 problems from doing it this way. Lots of ppl with clean swabs from me to back up the practice, me thinx
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Nichrome
Virus Factory
Registered: 12/17/18
Posts: 7,773
Loc: Zone 5
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Quote:
san pedro guy said:
Quick question:
If I drop a plate pin straight to grains, will it mess up the genetics if the cap opens or does it not matter because there are already spores in the cap by the time it forms?
thanks
That will vary a lot from culture to culture. Using mature plate pins as starter cultures has more often than not been consistent in producing some fine and vigorous fruiting cultures for me. It is as if the spores on the gills are designed by the host to improve and stay with the host. I love colony forming organisms.
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 Smellyhobbit
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Re: Ask Quick Questions Here [Re: Nichrome]
#27828282 - 06/20/22 06:40 AM (2 years, 9 months ago) |
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Quick question:
If none of my agar is performing from spore, LC, or live myc, what’s the most likely problem? It is MEA with a 8:8:400 malt:agar:water ratio.
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Healing Oakland
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Can you give me that in grams to mL?
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Healing Oakland
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Rotnpins
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I think its 8g/8g/400ml
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Smellyhobbit
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Re: Ask Quick Questions Here [Re: Rotnpins]
#27828609 - 06/20/22 12:10 PM (2 years, 9 months ago) |
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Correct, sorry. It’s 8g/8g/400ml agar/malt/water. Just sorta the universal beginner recipe here on the forum.
I have my plates in the incubator with my spawn. They’ll grow a little then stall. I’ll keep trying with better samples as I get a grow going, but if there’s anything off the top of your heads you think might be a problem I’d love to know. Otherwise I’ll continue to collect data and come back in a couple of months.
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Rotnpins
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Have any pictures?
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smalltalk_canceled
Babnik
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Re: Ask Quick Questions Here [Re: Rotnpins]
#27828624 - 06/20/22 12:18 PM (2 years, 9 months ago) |
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Does activated charcoal have nutrition that can contam?
How about granulated lime?
Are they both considered inert for our purposes ?
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SeaSalt
Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 1 year, 30 days
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Quick question: What do I do with an abundance of overgrown plates that are knotting and pinning? Is there a limit to how much agar I can inoculate a quart jar with?
My absurdly high contamination rate dropped a couple months ago, along with an increase in home temperature. Now, my home and refrigerator runneth over with 100+ plates. I'm just learning proper grain prep and haven't learned LI or LC. I currently have 28 oat jars inoculated with agar wedges and 36 more to modify.
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Healing Oakland
Mushroom Co-op
Registered: 06/03/22
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Last seen: 24 days, 18 hours
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Quote:
Smellyhobbit said:
Correct, sorry. It’s 8g/8g/400ml agar/malt/water. Just sorta the universal beginner recipe here on the forum.
I have my plates in the incubator with my spawn. They’ll grow a little then stall. I’ll keep trying with better samples as I get a grow going, but if there’s anything off the top of your heads you think might be a problem I’d love to know. Otherwise I’ll continue to collect data and come back in a couple of months.
Quick Answer:
The nutrient content at 0.02% is cool, I like a bit less, but it's likely not the nutrient profile that is messing you up to this degree.
Take you plates (and everything else) out of the incubator, they don't need to be at anything other than room temp. How hot is it in there? I could see this being a conversation if you live in a cold place with no HVAC, but it's June, even Canada is hot right now. Patience, and room temp is all you need. The incubator isn't your friend in this instance.
Quote:
SeaSalt said:
Quick question: What do I do with an abundance of overgrown plates that are knotting and pinning? Is there a limit to how much agar I can inoculate a quart jar with?
My absurdly high contamination rate dropped a couple months ago, along with an increase in home temperature. Now, my home and refrigerator runneth over with 100+ plates. I'm just learning proper grain prep and haven't learned LI or LC. I currently have 28 oat jars inoculated with agar wedges and 36 more to modify.
Quick Answer:
If you have too many plates and some are pinning, transfer the best looking plate pins to a new plate and you just cut a corner by not having to grow a whole tub to get a clone. These are already demonstrating a strong genetic profile in that they want to fruit under stress/less than ideal conditions.
I am all about learning agar, but it sounds like you are going overboard a little bit. I have run triple digit tub grows and didn't need that much agar sitting in my fridge. Also, you only need a tiny little piece of colonized agar to inoculate a jar, and the less agar you use to inoculate a jar the less chance you have of contaminants hitching a ride into your grain jar. Use small pieces first, I have put whole plates that were crisscross cut into a grain bag that I knew were clean to speed things up a bit, but generally not the way to go. Keep the sample size small, and keep practicing with agar to grain before attempting LI or LC. In fact if you get good at agar you don't even need to know that stuff as grain to grain transfers can quickly make you more spawn than you could ever handle. LI and LC are not beginner friendly, create extra contaminant vectors, and even experienced bulk cultivators can grow their whole career never doing anything but g2g. Not by any means shitting on LI or LC, plenty of people love it, but it doesn't sound like you're ready to jump to that yet. Keep up the good work!
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Healing Oakland
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Are you an established or aspiring cultivator in or near the Bay Area?
Healing Oakland is hiring. Shoot us a message.
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san pedro guy
Captain
Registered: 10/22/17
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i think it’s 2% not 0.02%
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Healing Oakland
Mushroom Co-op
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whoops, typo lol 
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Healing Oakland
An Entheogen Church Co-operative
Are you an established or aspiring cultivator in or near the Bay Area?
Healing Oakland is hiring. Shoot us a message.
This is a real place of employment with payroll, benefits, the whole nine.
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Sockadin
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 1
#27829052 - 06/20/22 06:00 PM (2 years, 9 months ago) |
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Quote:
Nichrome said:
Quick question,
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
No I think sunflower seeds offer alot of healthy fats most of the sunflower seeds are black oil sunflowers in almost every batch of wbs.
You know I'm a fan of Black Oil Sunflower seeds.
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Nichrome
Virus Factory
Registered: 12/17/18
Posts: 7,773
Loc: Zone 5
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Re: Ask Quick Questions, Get Quick Answers [Re: Sockadin]
#27829071 - 06/20/22 06:16 PM (2 years, 9 months ago) |
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Quote:
Sockadin said:
Quote:
Nichrome said:
Quick question,
Should someone remove sunflower seeds when using WBS for spawn that will be used directly and not expanded?
No I think sunflower seeds offer alot of healthy fats most of the sunflower seeds are black oil sunflowers in almost every batch of wbs.
You know I'm a fan of Black Oil Sunflower seeds.
Golden. 
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