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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: Cultivation General Discussion [Re: Nichrome]
#28211761 - 03/02/23 09:35 PM (10 months, 23 days ago) |
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Gotcha gotcha. Same dance different song.
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rumfor69
Bodhicitta Cultivator



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Re: Cultivation General Discussion [Re: Nichrome]
#28211763 - 03/02/23 09:38 PM (10 months, 23 days ago) |
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So I should use turkey stock concentrate instead of just turkey broth?
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Nichrome
I'm a torso!


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Re: Cultivation General Discussion [Re: rumfor69] 1
#28211766 - 03/02/23 09:40 PM (10 months, 23 days ago) |
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Hydrate coir with pee and vape off the ammonia over several weeks for best results. That's like 200 mg from an adult in a day. More if you're dying.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
Edited by Nichrome (03/02/23 09:44 PM)
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Nichrome] 1
#28211770 - 03/02/23 09:44 PM (10 months, 23 days ago) |
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As far as I understand, I'm not a chemist, But the fungi doesn't just assimilate l-Tryptophan from the environment, it's biosynthesised via sugars like Erythrose 4-phosphate. Otherwise all you'd have to do is dump tryptophan supplements into your sub.
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rumfor69
Bodhicitta Cultivator



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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#28211775 - 03/02/23 09:48 PM (10 months, 23 days ago) |
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Aren't we all dying all the time? How quick do I need to be dying to have the best pee for my coir?
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: rumfor69] 1
#28211776 - 03/02/23 09:48 PM (10 months, 23 days ago) |
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Faster?
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Nichrome
I'm a torso!


Registered: 12/17/18
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#28211785 - 03/02/23 09:56 PM (10 months, 23 days ago) |
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That's interesting that they synthesize tryptophan de novo. Still not sold they don't take it in from external sources though.
An article from a quick search regarding tryptophan and psilocybe metabolism. https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cplu.202000581
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Nichrome
I'm a torso!


Registered: 12/17/18
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Re: Cultivation General Discussion [Re: rumfor69] 1
#28211789 - 03/02/23 09:57 PM (10 months, 23 days ago) |
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Quote:
rumfor69 said: Aren't we all dying all the time? How quick do I need to be dying to have the best pee for my coir?
Like in a couple hours.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Smellyhobbit
Actual Retard



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Re: Cultivation General Discussion [Re: Nichrome] 1
#28211793 - 03/02/23 10:01 PM (10 months, 23 days ago) |
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I pee pure phosphate and tryptophan. Honestly it hurts but anything for mush cult.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Nichrome] 2
#28211794 - 03/02/23 10:02 PM (10 months, 23 days ago) |
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Yeah that's what I was referencing, seems like a well mapped pathway, but like I said, I'm not much of a chemist. Seems like too convenient/simplistic to just feed the colony tryptophan to increase potential potency.
I can barely handle mush as it is, I wouldn't want to eat mush all juiced up on tryptophan
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rumfor69
Bodhicitta Cultivator



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Re: Cultivation General Discussion [Re: Stipe-n Cap] 7
#28211802 - 03/02/23 10:13 PM (10 months, 23 days ago) |
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Nichrome
I'm a torso!


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Re: Cultivation General Discussion [Re: rumfor69] 1
#28211827 - 03/02/23 10:33 PM (10 months, 23 days ago) |
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HGH (Hongo Growth Hormone)?
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
Edited by Nichrome (03/02/23 10:34 PM)
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Adas
Lonely Dreamer



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Re: Cultivation General Discussion [Re: Nichrome]
#28211995 - 03/03/23 04:22 AM (10 months, 22 days ago) |
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Quote:
Nichrome said: L-tryptophan is the precursor that psilocybe mushrooms use to synthesize active alkaloids. That is known and the genes have been identified. Psilocybe mushrooms do not make their own tryptophan. They are heterotrophs and they metabolize tryptophan from external sources in order to synth alks.
It's not rocket science.

Im not entirely sure about this. Some mushrooms have been shown to be able to utilize ammonia or even ureic nitrogen. Which means they can synthesize amino acids from it. Heterotrophs just means they gotta feed on some decaying matter, not necessarily that they can't synth a certain amino acid.
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Re: Cultivation General Discussion [Re: Smellyhobbit] 1
#28212072 - 03/03/23 06:52 AM (10 months, 22 days ago) |
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Quote:
Smellyhobbit said: Which is why I hydrate coir with turkey broth.
Stop being perfect.
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🅃🄴🄰🄼 🄵🄾🄸🄻
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,401
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Quote:
sporemaster420 said:
Quote:
abductee said: I don't believe there's any correlation between grow time and potency, I believe potency is based on genetics.
Pretty sure I read the other day in a peer-reviewed article that potency varies within isolates according to growth conditions. So genetics may play a role, but so doesn’t the environment.
Pretty sure I wanna see that link or you're just making things up.
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🅃🄴🄰🄼 🄵🄾🄸🄻
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Stipe-n Cap


Registered: 08/04/12
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Re: Cultivation General Discussion [Re: Adas]
#28212106 - 03/03/23 07:27 AM (10 months, 22 days ago) |
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Quote:
Adas said: Some mushrooms have been shown to be able to utilize ammonia or even ureic nitrogen. Which means they can synthesize amino acids from it. Heterotrophs just means they gotta feed on some decaying matter, not necessarily that they can't synth a certain amino acid.
l-Tryptophan is de novo biosynthesed from d-erythrose-4-phosphate and phosphoenol pyruvate requiring 13 steps and 12 enzymes.
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cplu.202000581
The colony produces it's own Tryptophan.
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SirPsycho
Purple Belt in Google-Fu



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Re: Cultivation General Discussion [Re: LtLurker] 1
#28212286 - 03/03/23 10:15 AM (10 months, 22 days ago) |
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Quote:
LtLurker said:
Quote:
SirPsycho said:
Quote:
LadysKnight said: The Quickening

Am I the only one around here that thinks this is how tc tags should be given out? Gotta take out a current TC and claim their essence
Come on. Try and take it bro.
Ok 3:30 by the flagpole.
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Egon_Spengler
Ever lurkin never workin



Registered: 04/02/22
Posts: 2,294
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Re: Cultivation General Discussion [Re: SirPsycho] 2
#28212295 - 03/03/23 10:25 AM (10 months, 22 days ago) |
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Quote:
SirPsycho said:
Quote:
LtLurker said:
Quote:
SirPsycho said:
Quote:
LadysKnight said: The Quickening

Am I the only one around here that thinks this is how tc tags should be given out? Gotta take out a current TC and claim their essence
Come on. Try and take it bro.
Ok 3:30 by the flagpole.
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Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,491
Loc: Zone 5
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Re: Cultivation General Discussion [Re: Nichrome] 1
#28212386 - 03/03/23 11:33 AM (10 months, 22 days ago) |
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Quote:
Adas said:
Quote:
Nichrome said: L-tryptophan is the precursor that psilocybe mushrooms use to synthesize active alkaloids. That is known and the genes have been identified. Psilocybe mushrooms do not make their own tryptophan. They are heterotrophs and they metabolize tryptophan from external sources in order to synth alks.
It's not rocket science.

Im not entirely sure about this. Some mushrooms have been shown to be able to utilize ammonia or even ureic nitrogen. Which means they can synthesize amino acids from it. Heterotrophs just means they gotta feed on some decaying matter, not necessarily that they can't synth a certain amino acid.
Quote:
Nichrome said: That's interesting that they synthesize tryptophan de novo. Still not sold they don't take it in from external sources though.
An article from a quick search regarding tryptophan and psilocybe metabolism. https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cplu.202000581
I think "not entirely sure" Is what we are all saying....
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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bw86
Doesn't play well with others


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Re: Cultivation General Discussion [Re: Nichrome] 7
#28212402 - 03/03/23 11:40 AM (10 months, 22 days ago) |
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il'l tell you whut If you add pan myc to your (appropriate)substrate instead of cube myc you will get higher potency mushrooms.
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