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OfflineEugene Gesuale
Jar-Sniffer
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Registered: 04/12/20
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Re: Cultivation General Discussion [Re: dowodenum] * 2
    #28234068 - 03/17/23 05:49 PM (10 months, 8 days ago)

When talking about mushcult actually interferes with doing much cult….


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OfflineTight Lunchbox
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Re: Cultivation General Discussion [Re: Eugene Gesuale] * 2
    #28234617 - 03/18/23 05:09 AM (10 months, 7 days ago)

It has been said that talking about doing something uses the same resources as actually doing it.


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Offlineseekercc
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Registered: 05/27/22
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Re: Cultivation General Discussion [Re: Tight Lunchbox] * 1
    #28234843 - 03/18/23 10:33 AM (10 months, 7 days ago)

Is this blueish color OK?  It looks pretty black in the pic, but it looks dark blue



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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
Re: Cultivation General Discussion [Re: seekercc] * 2
    #28234846 - 03/18/23 10:34 AM (10 months, 7 days ago)

Quote:

seekercc said:
Is this blueish color OK?  It looks pretty black in the pic, but it looks dark blue






It's fine. Bruising.


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Offlineseekercc
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Re: Cultivation General Discussion [Re: Way]
    #28234902 - 03/18/23 11:12 AM (10 months, 7 days ago)

The dark blue on the cakes OK too?


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InvisibleWay
The


Registered: 01/14/23
Posts: 4,336
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Re: Cultivation General Discussion [Re: seekercc] * 1
    #28234929 - 03/18/23 11:35 AM (10 months, 7 days ago)

Yeah, that's quite a bit of bruising though. More than I've ever seen on cakes. You been slapping them around?

Most likely from drying out though. Perhaps rough handling or too much fresh air. I wouldn't fuck with it too much since those cakes probably don't have much life left in them. One on the top right is all aborts.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.


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Invisibleabductee
Time
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Registered: 05/07/15
Posts: 2,224
Loc: Canada
Re: Cultivation General Discussion [Re: seekercc]
    #28234932 - 03/18/23 11:37 AM (10 months, 7 days ago)

Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.


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OfflineTriptress
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Registered: 02/16/23
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Re: Cultivation General Discussion [Re: abductee]
    #28234949 - 03/18/23 11:52 AM (10 months, 7 days ago)

I use a vacuum sealer  on .5 micron bags and inject through the bag with LC and then put duct tape over the hole as soon as I pull the needle out in the SAB I know people have opened bags in the SAB with success but to me that sounds like stuff that should be done with a flowhood


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Invisibleabductee
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Re: Cultivation General Discussion [Re: Triptress]
    #28234961 - 03/18/23 12:03 PM (10 months, 7 days ago)

OK, thanks alot.


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Invisibleabductee
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Re: Cultivation General Discussion [Re: Triptress]
    #28234968 - 03/18/23 12:08 PM (10 months, 7 days ago)

Do you order online, any links you can please share for your bags, I seen a lot of options.


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OfflinePBJ710
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Registered: 07/05/19
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Re: Cultivation General Discussion [Re: abductee]
    #28234972 - 03/18/23 12:12 PM (10 months, 7 days ago)

Quote:

abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.




You should assume that any LC is contaminated and verify it's cleanliness on agar before using it.  Just because you paid someone for it doesn't mean that it's clean.  If you're just going to inject LC it's not a big deal to use a SAB as you can leave the bag rolled up and just inject through the side - If you want to G2G to bags, a hood or FFU is pretty much required to be consistently successful.

Myers Mushrooms has good pricing on partial/full cases.  T series bags are .2u filters - just pick the size that you want to run.  3T's are commonly used with 3-5lbs of spawn for tubs

<edit - corrected filter info>


Edited by PBJ710 (03/19/23 08:30 AM)


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OnlineYoshiTrainer
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Registered: 04/30/22
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Re: Cultivation General Discussion [Re: abductee] * 1
    #28234980 - 03/18/23 12:17 PM (10 months, 7 days ago)

Quote:

abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.




You don't need an impulse sealer, they are VERY convenient but have their disadvantages too. You can get away with folding the top down a couple times and add a spring clamp or two. Some people use tape also. You could do your SAB work with tape/clamps then seal later if you really wanted. For LC syringes, I'd recommend larger gauge needles, syringe size is up to your needs.

Good luck!


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Invisibleabductee
Time
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Registered: 05/07/15
Posts: 2,224
Loc: Canada
Re: Cultivation General Discussion [Re: YoshiTrainer]
    #28235003 - 03/18/23 12:30 PM (10 months, 7 days ago)

Thanks everyone for your input.
I'll be making my own lc for the first time and I'll definitely check it out on agar first.👌 lc should be easy, it's just getting that first hand experience.


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OfflineSoloist
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Registered: 02/10/22
Posts: 922
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Re: Cultivation General Discussion [Re: abductee]
    #28235105 - 03/18/23 01:53 PM (10 months, 7 days ago)

So it’s been 11 days since I nocc’d up some WBS agar with Texas yellow cap.
2 prints, one labeled “dirty”…which I can attest haha, all but one of twelve have bacteria and/or some pink shit. The 12 from the second print are all nice and contam free.

But the dirty plates seem to have taken off a lot faster whereas the clean plates are still looking kinda weak in comparison
-all the plates from both prints are  tomenstose at this point. (this is my first go and I’m not sure how myc should look at this stage)

My question is this, What would you guys do??
Make some T1’s from the thick but dirty plates or let the clean ones ride out longer and T1 from there…or both?


“Dirty” print


Clean print


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Edited by Soloist (03/18/23 03:15 PM)


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OfflineScrewup
Googles your dumb questions
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Re: Cultivation General Discussion [Re: Soloist]
    #28235118 - 03/18/23 02:02 PM (10 months, 7 days ago)

None of those look good. Those ones that have no white are bacteria.


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OfflineSoloist
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Re: Cultivation General Discussion [Re: Screwup]
    #28235161 - 03/18/23 02:21 PM (10 months, 7 days ago)

I have to say that even though this is my first go, I did plenty of research and test runs beforehand, including intentionally contaminating agar so I knew what to look for.
The ones with “no white”, actually do have white, it’s just very very thin/fine (that sheen on top was first indication of germination) and presented on all the plates prior to noticeable fuzz.
The contaminated plates actually presented their contams days before any signs of myc.
My gut is telling me that my wbs agar recipe may have something to do with the fuzzy tomentose myc., or like I said, just don’t know how myc behaves this young.


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  🌕🌖🌗🌘🌑🌒🌓🌔🌕
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapéh Crafters
Trade and wish list
🍄👀MO🍄👀
It’s time to ghost this place✌🏻


Edited by Soloist (03/18/23 03:10 PM)


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OfflineScrewup
Googles your dumb questions
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Re: Cultivation General Discussion [Re: Soloist] * 1
    #28235165 - 03/18/23 02:21 PM (10 months, 7 days ago)

That shine/sheen is bacteria. Likely slime bacteria or something idk. Regardless it’s bacteria and I get it too.


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Invisiblenatedawgnow
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Registered: 02/09/15
Posts: 8,939
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Trusted Cultivator
Re: Cultivation General Discussion [Re: PBJ710] * 1
    #28235166 - 03/18/23 02:22 PM (10 months, 7 days ago)

Quote:

PBJ710 said:
Quote:

abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.




You should assume that any LC is contaminated and verify it's cleanliness on agar before using it.  Just because you paid someone for it doesn't mean that it's clean.  If you're just going to inject LC it's not a big deal to use a SAB as you can leave the bag rolled up and just inject through the side - If you want to G2G to bags, a hood or FFU is pretty much required to be consistently successful.

Myers Mushrooms has good pricing on partial/full cases.  T series bags are .5u filters - just pick the size that you want to run.  3T's are commonly used with 3-5lbs of spawn for tubs



T series bags are .2 filters A series bags are .5


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OfflineSoloist
Indigenous Human

Registered: 02/10/22
Posts: 922
Loc: Suburban hell
Last seen: 1 hour, 44 minutes
Re: Cultivation General Discussion [Re: Screwup]
    #28235249 - 03/18/23 03:05 PM (10 months, 7 days ago)

Quote:

Screwup said:
That shine/sheen is bacteria. Likely slime bacteria or something idk. Regardless it’s bacteria and I get it too.




Okay.
What’s your move?


--------------------
Embrace your darkness,
For without it,
Your light can never
truly exist.

                  🌕🌖🌗🌘🌑🌒🌓🌔🌕
                          The Earth And I
89g fresh Gymnopilus subspectibilis
Rapéh Crafters
Trade and wish list
🍄👀MO🍄👀
It’s time to ghost this place✌🏻


Extras: Filter Print Post Top
OfflineScrewup
Googles your dumb questions
Trans-female

Registered: 01/27/22
Posts: 6,301
Last seen: 6 hours, 1 minute
Re: Cultivation General Discussion [Re: Soloist] * 1
    #28235258 - 03/18/23 03:09 PM (10 months, 7 days ago)

Personally I don’t like any of those cups. I think you could attempt water agar to outrun the bacteria but if there’s mold you’d still have ride alongs. Let someone more experienced chime in. At the very least you’re gonna need a few more transfer.


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