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Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,491
Loc: Zone 5
Last seen: 20 hours, 24 minutes
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Re: Cultivation General Discussion [Re: bw86] 4
#28212413 - 03/03/23 11:46 AM (10 months, 22 days ago) |
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Must be because they are metabolizing the tryptophan that was created de novo in the pan myc...... 
I kid I kid. Some of the most potent cubes I've seen grew from a ziplock full of stem butts that was for shits and giggles. Cannibals.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
Last seen: 5 hours, 17 minutes
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Re: Cultivation General Discussion [Re: Nichrome] 1
#28212441 - 03/03/23 12:13 PM (10 months, 22 days ago) |
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Quote:
Nichrome said: I think "not entirely sure" Is what we are all saying....
Yea Im pretty sure they may as well be doing both. Because, ultimately.. why not? 
We, too, can synthesize some amino acids, but we also take them from food.
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Adas] 3
#28212463 - 03/03/23 12:31 PM (10 months, 22 days ago) |
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Whatever the reality is, macro tryptophan supplementation seems to be equally as ridiculous as other forms of over supplementation. The reddit cult member rationalization of amending substrates or spawn with x fad supplement is owed to a misunderstanding of the nutritional requirements of fungi.
It's clear that tryptophan is biosynthesised, any exogenous tryptophan would be very unlikey to exceed the amounts found in cereal grains utilized for spawn, that's IF the organism can even metabolize exogenous tryptophan, yet to be determined. The same applies to: calcium, magnesium, protein, b vitamins, etc.
"But, why cant we just add more of x for moar mush, why not just add y for more potency"
Because fungi are microorganisms, not flowering plants, or growing mammals. Cereal grains are dense with macro and micronutrients. Fungal biochemistry is not accelerated by the presence of excess resources, it's not linear in the sense that more of any single ingredient necessarily produces some arbitrary "boost" in biological efficiency or potency.
Cereal grains are used to grow full sized Ungulates, livestock, but are somehow insufficient for fungi? Show me a spent tub where the grains have been completely catabolized by the colony and I'll change my mind. There's always plenty of food left on their plates 
Edited by Stipe-n Cap (03/03/23 01:33 PM)
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Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,491
Loc: Zone 5
Last seen: 20 hours, 24 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 2
#28212551 - 03/03/23 01:44 PM (10 months, 22 days ago) |
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Most things that take in tryptophan do not do it in a simple cut and dry way. This is why the theory of adding X to substrate B always plays out as not working and that is not being suggest here. In many things all sorts of factors for absorbing tryptophan including cellular growth stages, PH, presence of other chemicals needed for taking it in, moving it, storing it, and transforming it, water availability, specific inhibitors like other aminos that compete for the same channels, oxygen ratios etc etc. It is a complex topic and frankly anyone thinking about it as simply as "adding tryptophan will or won't" is very much thinking about it "simply" with a "simple mind". Using simple math while trying to describe gravity is a fools task that only a real savant might pull off.
There are credited peer reviewed studies (if those garbage titles of circle jerk idiotic absolutism are appealing or mean something) with fungi that convert tryptophan to indole alkaloids that chemically are very similar to psiloxxx. Indeed it is shown that tryptophan if applied at the right time and with the right amount and proportions can be taken in and converted in both liquid and solid state fermentation. The levels absorbed and stored and converted were monitored over hours and days. It's mode was dependent on such factors as genetics, time, chemicals, temperatures, and stages of cellular development. It is really fascinating stuff. It was also shown that certain chemicals blocked the absorption of tryptophan completely, although those cultures still had a tryptophan content as well as alkaloid production suggesting de novo synthesis.
I am not saying adding tryptophan will increase potency. It's just not that simple. Tryptophan is a piece of the puzzle is all and like Adas pointed out it is highly unlikely they do not do both. Like how we can't prove that extra terrestrial life exists but the likelihood that it doesn't is exponentially slim. Understanding what life is would be a first step in identifying it.
It's not as simple as adding an ingredient. Nor am I trying to say so. I'm talking about algebra here not 1+2=3. There is an equation and unknown variables. Tryptophan being a "known factor". Specifically l-tryptophan which has only recently become available on the market. All the tryptophan talk in the forums from the past I have found were using non usable forms of tryptophan and were just absolutely shitty "experiments".
No conclusion.
What is Reddit?
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Cultivation General Discussion [Re: Nichrome] 3
#28212572 - 03/03/23 01:57 PM (10 months, 22 days ago) |
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Lel, "crushing up my vitamins, just an experiment bro" 
Speaking of aliens, I watched this very interesting video covering protein folding and Leventhals Paradox, very cool.
For those interested:
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Capitan_Pacheco
Stranger

Registered: 02/15/23
Posts: 96
Last seen: 9 days, 14 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 1
#28212574 - 03/03/23 01:59 PM (10 months, 22 days ago) |
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Hello. I really want to read this whole thread  
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NFLProof
Sponge



Registered: 05/31/18
Posts: 893
Loc: In the ether
Last seen: 2 days, 2 hours
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Aside from reading everything on here and soak in as much as I can, does anyone have a book suggestion or two that explains the beginning/basics of mycology? Like a mycology for dummies or something?
-------------------- Yeah, yeah.
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,390
Loc: Fuck off pig!🐷
Last seen: 37 minutes, 25 seconds
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Re: Cultivation General Discussion [Re: NFLProof] 1
#28212594 - 03/03/23 02:20 PM (10 months, 22 days ago) |
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Quote:
NFLProof said: Aside from reading everything on here and soak in as much as I can, does anyone have a book suggestion or two that explains the beginning/basics of mycology? Like a mycology for dummies or something?
Find some TCs on here that have grows you admire and read their journals.
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Capitan_Pacheco
Stranger

Registered: 02/15/23
Posts: 96
Last seen: 9 days, 14 hours
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Quote:
San Pedro Girl said:
Quote:
NFLProof said: Aside from reading everything on here and soak in as much as I can, does anyone have a book suggestion or two that explains the beginning/basics of mycology? Like a mycology for dummies or something?
Find some TCs on here that have grows you admire and read their journals.
PSILOCYBIN. MAGIC MUSHROOM GROWER'S GUIDE. A Handbook for Psilocybin Enthusiasts. O.T. OSS & O.N. OERIC
I am new here. Can I post a link of the pdf book?
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
Last seen: 6 days, 2 hours
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 2
#28212651 - 03/03/23 03:25 PM (10 months, 22 days ago) |
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Quote:
Stipe-n Cap said: As far as I understand, I'm not a chemist, But the fungi doesn't just assimilate l-Tryptophan from the environment, it's biosynthesised via sugars like Erythrose 4-phosphate. Otherwise all you'd have to do is dump tryptophan supplements into your sub.
I knew I wasn't crazy and the only one. We figured so much through imperfect, anecdotal experiments before scientists confirm it. If we could boost potency that easy, I imagine we'd be doing it
@ captain. It was published in 76. Likely more wrong than correct at this point cause its so outdated.
Edited by LtLurker (03/03/23 03:43 PM)
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Capitan_Pacheco
Stranger

Registered: 02/15/23
Posts: 96
Last seen: 9 days, 14 hours
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Re: Cultivation General Discussion [Re: LtLurker]
#28212679 - 03/03/23 03:45 PM (10 months, 22 days ago) |
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Quote:
LtLurker said:
Quote:
Stipe-n Cap said: As far as I understand, I'm not a chemist, But the fungi doesn't just assimilate l-Tryptophan from the environment, it's biosynthesised via sugars like Erythrose 4-phosphate. Otherwise all you'd have to do is dump tryptophan supplements into your sub.
I knew I wasn't crazy and the only one.
@ captain. It was published in 76. Likely more wrong than correct at this point cause its so outdated.
Yes, it may be outdated. But it is a nice read for any begginer.
Psilocybin mushrooms of the world, by Paul Stamets may be more actual.
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abductee
Time



Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#28212707 - 03/03/23 03:59 PM (10 months, 22 days ago) |
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But they do exist..lol
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cozmyc
gentle modern ape



Registered: 06/20/21
Posts: 2,131
Last seen: 14 hours, 20 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 3
#28212736 - 03/03/23 04:09 PM (10 months, 22 days ago) |
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Quote:
Stipe-n Cap said: Lel, "crushing up my vitamins, just an experiment bro" 
Speaking of aliens, I watched this very interesting video covering protein folding and Leventhals Paradox, very cool.
For those interested:
Such an excellent channel. Wonderful person.
-------------------- You're conscious population 2 stardust ---------------------- and that's valuable
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,907
Loc: Rent free in your head
Last seen: 3 hours, 36 minutes
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Re: Cultivation General Discussion [Re: Stipe-n Cap] 4
#28212749 - 03/03/23 04:16 PM (10 months, 22 days ago) |
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Quote:
Stipe-n Cap said: Lel, "crushing up my vitamins, just an experiment bro" 
Speaking of aliens, I watched this very interesting video covering protein folding and Leventhals Paradox, very cool.
For those interested:
That's so fucking weird. I watched that same video on the way to work this morning.
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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milkboy
Child



Registered: 05/01/21
Posts: 2,299
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Re: Cultivation General Discussion [Re: SirPsycho] 4
#28212752 - 03/03/23 04:18 PM (10 months, 22 days ago) |
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Do we all watch antons videos?
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 23 hours, 3 minutes
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Re: Cultivation General Discussion [Re: milkboy]
#28212776 - 03/03/23 04:27 PM (10 months, 22 days ago) |
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Wait wait wait....what if I grow mushrooms, on mushrooms, from mushrooms, that have mushrooms grown from them...will they uptake the maximize threshold!?!?
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phenyl
Stupid person



Registered: 09/03/22
Posts: 328
Loc: Bat Country
Last seen: 4 hours, 8 minutes
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Re: Cultivation General Discussion [Re: rumfor69]
#28212802 - 03/03/23 04:46 PM (10 months, 22 days ago) |
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Quote:
rumfor69 said: Wait wait wait....what if I grow mushrooms, on mushrooms, from mushrooms, that have mushrooms grown from them...will they uptake the maximize threshold!?!?
scientists hate him
-------------------- The fool who persists in his folly will become wise.
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SeaSalt



Registered: 12/08/21
Posts: 495
Loc: PNW WWA
Last seen: 4 months, 26 days
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Re: Cultivation General Discussion [Re: Stipe-n Cap]
#28212903 - 03/03/23 05:52 PM (10 months, 22 days ago) |
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Quote:
Stipe-n Cap said:
Quote:
SirPsycho said:
Quote:
LadysKnight said: The Quickening

Am I the only one around here that thinks this is how tc tags should be given out? Gotta take out a current TC and claim their essence
Well, this is definitely how it works. Ever notice how eats, hamloaf, cron, and others haven't been around lately....
New TC' come in, the old ones are never heard from again 
Yeah, where is Cronicr? It's like he fell off the face of the earth (or in love).
-------------------- Avatar by Sol_Exposure
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,390
Loc: Fuck off pig!🐷
Last seen: 37 minutes, 25 seconds
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Re: Cultivation General Discussion [Re: SeaSalt]
#28212924 - 03/03/23 06:01 PM (10 months, 22 days ago) |
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Quote:
SeaSalt said:
Quote:
Stipe-n Cap said:
Quote:
SirPsycho said:
Quote:
LadysKnight said: The Quickening

Am I the only one around here that thinks this is how tc tags should be given out? Gotta take out a current TC and claim their essence
Well, this is definitely how it works. Ever notice how eats, hamloaf, cron, and others haven't been around lately....
New TC' come in, the old ones are never heard from again 
Yeah, where is Cronicr? It's like he fell off the face of the earth (or in love).
I bet most people on here are like me. Nobody here actually knows meso when my high blood pressure finally kills me, I’ll just poof from here!
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LtLurker
Lost Sailor



Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
Last seen: 6 days, 2 hours
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Quote:
Capitan_Pacheco said:
Quote:
LtLurker said:
Quote:
Stipe-n Cap said: As far as I understand, I'm not a chemist, But the fungi doesn't just assimilate l-Tryptophan from the environment, it's biosynthesised via sugars like Erythrose 4-phosphate. Otherwise all you'd have to do is dump tryptophan supplements into your sub.
I knew I wasn't crazy and the only one.
@ captain. It was published in 76. Likely more wrong than correct at this point cause its so outdated.
Yes, it may be outdated. But it is a nice read for any begginer.
Psilocybin mushrooms of the world, by Paul Stamets may be more actual.
Stamens is actually the worst. While peers of his era are limited by the time their in but otherwise did good work, stamets just made shit up from "common sense" and wrote them like facts in his books without any basic experimentation. He even named things as if they were new species when they weren't; genetics tells us this for sure now, but most of his contemporaries always said they were likely the same as known species and likely just regional varieties at most.
Now a days he says horrible lies like oysters curing a friends cancer, or psych shrooms curing deafness. Oh and of course he happens to sell these miracle cures. His behavior is much more akin to a con artist or snake oil salesman than a researcher.
I cant name a great current book. You can learn alot just by googling and surfing around. Older books aren't exactly terrible, you just gotta keep in mind there's gonna be things wrong.
Edited by LtLurker (03/03/23 07:08 PM)
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