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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Cultivation General Discussion [Re: abductee] * 1
    #28234980 -

abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.


You don't need an impulse sealer, they are VERY convenient but have their disadvantages too. You can get away with folding the top down a couple times and add a spring clamp or two. Some people use tape also. You could do your SAB work with tape/clamps then seal later if you really wanted. For LC syringes, I'd recommend larger gauge needles, syringe size is up to your needs.

Good luck!


--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Re: Cultivation General Discussion [Re: YoshiTrainer]
    #28235003 -

Thanks everyone for your input.
I'll be making my own lc for the first time and I'll definitely check it out on agar first.๐Ÿ‘Œ lc should be easy, it's just getting that first hand experience.

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Re: Cultivation General Discussion [Re: abductee]
    #28235105 -

So itโ€™s been 11 days since I noccโ€™d up some WBS agar with Texas yellow cap.
2 prints, one labeled โ€œdirtyโ€โ€ฆwhich I can attest haha, all but one of twelve have bacteria and/or some pink shit. The 12 from the second print are all nice and contam free.

But the dirty plates seem to have taken off a lot faster whereas the clean plates are still looking kinda weak in comparison
-all the plates from both prints are  tomenstose at this point. (this is my first go and Iโ€™m not sure how myc should look at this stage)

My question is this, What would you guys do??
Make some T1โ€™s from the thick but dirty plates or let the clean ones ride out longer and T1 from thereโ€ฆor both?


โ€œDirtyโ€ print


Clean print


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
โ€ข89g fresh subspectibilis teaโ€ข
โ€ขRapรฉh Craftersโ€ข
โ€ข Gift yourself a healthy dose of Self banningโ€ข

Edited by Soloist (03/18/23 05:15 PM)

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Re: Cultivation General Discussion [Re: Soloist]
    #28235118 -

None of those look good. Those ones that have no white are bacteria.


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Cultivation General Discussion [Re: Screwup]
    #28235161 -

I have to say that even though this is my first go, I did plenty of research and test runs beforehand, including intentionally contaminating agar so I knew what to look for.
The ones with โ€œno whiteโ€, actually do have white, itโ€™s just very very thin/fine (that sheen on top was first indication of germination) and presented on all the plates prior to noticeable fuzz.
The contaminated plates actually presented their contams days before any signs of myc.
My gut is telling me that my wbs agar recipe may have something to do with the fuzzy tomentose myc., or like I said, just donโ€™t know how myc behaves this young.


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
โ€ข89g fresh subspectibilis teaโ€ข
โ€ขRapรฉh Craftersโ€ข
โ€ข Gift yourself a healthy dose of Self banningโ€ข

Edited by Soloist (03/18/23 05:10 PM)

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Re: Cultivation General Discussion [Re: Soloist] * 1
    #28235165 -

That shine/sheen is bacteria. Likely slime bacteria or something idk. Regardless itโ€™s bacteria and I get it too.


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Cultivation General Discussion [Re: PBJ710] * 1
    #28235166 -

PBJ710 said:
abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.


You should assume that any LC is contaminated and verify it's cleanliness on agar before using it.  Just because you paid someone for it doesn't mean that it's clean.  If you're just going to inject LC it's not a big deal to use a SAB as you can leave the bag rolled up and just inject through the side - If you want to G2G to bags, a hood or FFU is pretty much required to be consistently successful.

Myers Mushrooms has good pricing on partial/full cases.  T series bags are .5u filters - just pick the size that you want to run.  3T's are commonly used with 3-5lbs of spawn for tubs


T series bags are .2 filters A series bags are .5


--------------------

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Re: Cultivation General Discussion [Re: Screwup]
    #28235249 -

Screwup said:
That shine/sheen is bacteria. Likely slime bacteria or something idk. Regardless itโ€™s bacteria and I get it too.


Okay.
Whatโ€™s your move?


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
โ€ข89g fresh subspectibilis teaโ€ข
โ€ขRapรฉh Craftersโ€ข
โ€ข Gift yourself a healthy dose of Self banningโ€ข

Extras: Filter Print Post Top
Re: Cultivation General Discussion [Re: Soloist] * 1
    #28235258 -

Personally I donโ€™t like any of those cups. I think you could attempt water agar to outrun the bacteria but if thereโ€™s mold youโ€™d still have ride alongs. Let someone more experienced chime in. At the very least youโ€™re gonna need a few more transfer.


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: Cultivation General Discussion [Re: Soloist]
    #28235308 -

The first pic , i mean second down between 8 and 7 o'clock before the two sectors meet. Take the smallest of sliver.

Edited by abductee (03/18/23 07:30 PM)

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Re: Cultivation General Discussion [Re: abductee]
    #28235321 -

Or the tomatoes growth at 11 12 oclock in the middle of the patch. I saw a guy take different transfers and the tomatoes or how it's spelled grew out rhizo.

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Re: Cultivation General Discussion [Re: natedawgnow]
    #28235643 -

natedawgnow said:
PBJ710 said:
abductee said:
Hey guys, I've been wanting to start using bags. Is it a pain to work with in the sab, should I get an impulse sealer or does folding and tape work fine for spawn bags? Anything to avoid when buying syringes for liquid culture?
Thanks for any tips or info.


You should assume that any LC is contaminated and verify it's cleanliness on agar before using it.  Just because you paid someone for it doesn't mean that it's clean.  If you're just going to inject LC it's not a big deal to use a SAB as you can leave the bag rolled up and just inject through the side - If you want to G2G to bags, a hood or FFU is pretty much required to be consistently successful.

Myers Mushrooms has good pricing on partial/full cases.  T series bags are .5u filters - just pick the size that you want to run.  3T's are commonly used with 3-5lbs of spawn for tubs


T series bags are .2 filters A series bags are .5


Doh!  Thanks for correcting that

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Re: Cultivation General Discussion [Re: Way]
    #28235738 -

Way said:
Yeah, that's quite a bit of bruising though. More than I've ever seen on cakes. You been slapping them around?

Most likely from drying out though. Perhaps rough handling or too much fresh air. I wouldn't fuck with it too much since those cakes probably don't have much life left in them. One on the top right is all aborts.


I've tried to be gentle, just picked them up a few times to harvest, & they did fall over once or twice, that's a pretty short fall tho.  The've only been sprouting for a week.  I thought maybe it was too humid, I've had it at 99%, or close most of the time.  Temp has been 68-77.

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Re: Cultivation General Discussion [Re: seekercc]
    #28235793 -

hi everyone,

First time going to rye.

Can someone recommend a good writeup for haw to prep rye for 1 quart mason jars that will then be going to shoeboxes?

I looked at willywonka's and CH's, wondering if there is a more recent approach.

I have two more questions:
1. Do I drop only one wedge per quart jar or do I do a tigerDrop and dump a whole plate?

2. Lids on jars. I see some of you have lids with some type of seal or valve or something.
Can I proceed with normal lids or will I have to modify something?

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Re: Cultivation General Discussion [Re: drVetker] * 7
    #28235959 -

Rye and wheat are similarly done for me.  Wheat just gets cooked longer.  Pretty easyโ€ฆ measure out between 1 - 1 1/8 cup dry grain (depending on how much you want each qt filled.  Rinse the grains and drain off a few times until the water is clear. 

Bring 3x the amount of water to grains to a boil with the grains in the water.  Once boiling, reduce to simmer and cook until you see a burst grain or two.  With rye I think it was ~30 mins.  With wheat itโ€™s 38-40 mins.  I strain it and rinse with hot water and then cold but a lot of ppl just let it steam dry but I like to rinse the starches off the grains so they donโ€™t stick as much after pcโ€™ing.  I let sit in the strainer for ~30 mins and shake the water out the bottom a few times and then load the jars.  You should look into wheat too.  I like it over rye and itโ€™s usually cheaper.  Hard red winter wheat is my go to

This is what my wheat looks like 4 days after making it.  I prefer a little more hydration than a lot of ppl so you can drop those times as needed


You can use as much inocate as you want.  The more you use, the faster the jar will get done colonizing.

Lots of ppl use unmodded lids.  I hear the leak proof gray lids work better than the cheap white ones.  You just crack the lid for GE. Check out pastyโ€™s lid tek

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Re: Cultivation General Discussion [Re: fahtster]
    #28236128 -

fahtster said:
Rye and wheat are similarly done for me.  Wheat just gets cooked longer.  Pretty easyโ€ฆ measure out between 1 - 1 1/8 cup dry grain (depending on how much you want each qt filled.  Rinse the grains and drain off a few times until the water is clear.
.
.
.




This is super, thank you very much sir.

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Re: Cultivation General Discussion [Re: Screwup]
    #28240340 -

Screwup said:
Personally I donโ€™t like any of those cups. I think you could attempt water agar to outrun the bacteria but if thereโ€™s mold youโ€™d still have ride alongs. Let someone more experienced chime in. At the very least youโ€™re gonna need a few more transfer.


Funny you mentioned water agar. I just read up on this a few days prior and realized the valueโ€ฆ.had been meaning to mix up a round for about a week.
..Poured my first batch of WA last night and did about 2 dozen T1โ€™s this afternoon.

The germ plates smelled nice and mushroomy so I know Iโ€™m at least on the right course. Saving a few in case Plins decide to pop off, hopefully get my feet wet with that too.

Thanks for the advice.

abductee said:
The first pic , i mean second down between 8 and 7 o'clock before the two sectors meet. Take the smallest of sliver.


abductee said:
Or the tomatoes growth at 11 12 oclock in the middle of the patch. I saw a guy take different transfers and the tomatoes or how it's spelled grew out rhizo.


Thanks for the advice.
Being that the plates were all a mess haha I just transferred from the densest areas. Tried to avoid the big blotches of bacteria and whatever the pink shit was as best I could.


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
โ€ข89g fresh subspectibilis teaโ€ข
โ€ขRapรฉh Craftersโ€ข
โ€ข Gift yourself a healthy dose of Self banningโ€ข

Edited by Soloist (03/21/23 09:50 PM)

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Re: Cultivation General Discussion [Re: Soloist] * 3
    #28240955 -

Awesomeness ๐Ÿ€.
I got my first flushes coming in nicely of my Texas yellow caps. I didn't have any extra bags so I went liner free and my monos got a bunch of side shrooms, no biggie. They will get harvest probably tonight, since they are mostly ready on the sides the pins shouldn't be disturbed too much in the center and get a nice follow up first flush.


Seems I ran out of upload space..
These were spawned with jars that showed signs of bacteria.

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Re: Cultivation General Discussion [Re: abductee]
    #28241069 -

abductee said:
Awesomeness ๐Ÿ€.
I got my first flushes coming in nicely of my Texas yellow caps. I didn't have any extra bags so I went liner free and my monos got a bunch of side shrooms, no biggie. They will get harvest probably tonight, since they are mostly ready on the sides the pins shouldn't be disturbed too much in the center and get a nice follow up first flush.


Seems I ran out of upload space..
These were spawned with jars that showed signs of bacteria.


I look forward to see your updates.


--------------------
For it is only Darkness that crafts one to thrive in the Light. As illumination of the soul, its brilliance unmatched.
               
                    ๐ŸŒ•๐ŸŒ–๐ŸŒ—๐ŸŒ˜๐ŸŒ‘๐ŸŒ’๐ŸŒ“๐ŸŒ”๐ŸŒ•
  :sporedrop:Gymnopilus subspectibilis grow log:sporedrop:             
โ€ข89g fresh subspectibilis teaโ€ข
โ€ขRapรฉh Craftersโ€ข
โ€ข Gift yourself a healthy dose of Self banningโ€ข

Extras: Filter Print Post Top
Re: Cultivation General Discussion [Re: Soloist]
    #28241133 -

Hello friends. I'd be hugely grateful for some advice on my situation -

I've got this LC test plate which I poured LC onto a week ago & decided it looked healthy enough to test on some jars..

I was eager to go so I PC'd & inoculated 7 jars of millet 5 days ago with said LC & these are the most active so far.

Do you think this growth is suspicious on either the agar or millet? It'd save me a lot of time to find out from someone with a better idea than me if they look potentially contaminated.

Cheers for any help, images below.

edit: that's a great quote in your sig Soloist, true words.


Edited by stockw (03/22/23 01:18 PM)

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Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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