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OfflineYoshiTrainer
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Drying agar and grains using parchment paper. * 15
    #28253223 - 03/29/23 04:13 PM (9 months, 25 days ago)

Drying grains and agar is a good way to store and share genetics. It gives you the ability to store clones or even genetics that you're unable to get spores from. By using parchment paper, I've reduced my drying times significantly while still maintaining clean cultures. I first worked with parchment paper' while making ice water hash. You'd put your wet hash in parchment paper, put cardboard around it and a weight on top. The moisture would pass through the paper and be absorbed by the cardboard. I did something pretty similar with the agar and grains.

First I cut and prefolded the parchment paper with double or triple folds on the edges and tightly creased. I wrapped my pouches in foil and baked 250F for 60min. This may be unnecessary but I didn't want to take chances. When it was time, I shook the donor jar to break up the grains, removed a pouch from the foil, opened it up and poured the grains in. I closed the pouch up then, broke the rest of the grain clumps so they fit into a single layer. I wrapped the pouch in a double layer of paper towels and placed a 1/2 pint large mouthed jar on top. I swapped out the paper towels every other day because they'd get damp. It took 7 days for the agar and grains to dry.

The agar was dried much the same way as the grains above. The agar was cut down the center. Then, it was folded in half with the myc touching myc and placed in a parchment pouch to dry.

I also made jar lids with parchment paper, held in place with metal bands. These had less grains but took about 10 days to dry.



The agar and grains were dried open air, in a file box, being pressed by jars of PF Tek.  What made me most optimistic was the fact the agar stained the inside of the parchment paper but not the outside nor the paper towels. 




I placed a dried grain to agar and a flake of dried mycelium to agar too. After about 10 days, both look pretty healthy and strong.





I was originally going to store some dry grains longterm in 5ml cyrovials maybe with some wine preserving gas? I'm thinking now, I may just vacuum seal them?



Edited by YoshiTrainer (05/29/23 10:45 AM)


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer]
    #28266846 - 04/07/23 12:14 PM (9 months, 16 days ago)

PEC4 agar, started drying 4/3 finished today 4/7. I hope to test it on some fresh agar in the next few days.



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InvisiblefahtsterM
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 1
    #28266926 - 04/07/23 01:18 PM (9 months, 16 days ago)

Missed this.  Noice :headbanger:


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InvisibleShrimps
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Re: Drying agar and grains using parchment paper. [Re: fahtster] * 1
    #28266930 - 04/07/23 01:21 PM (9 months, 16 days ago)

Awesome write up Yoshi! Can't wait to see how this turns out :laugh:


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InvisibleScemilope
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Re: Drying agar and grains using parchment paper. [Re: Shrimps] * 1
    #28276104 - 04/13/23 03:54 PM (9 months, 10 days ago)

Nice write up. Thanks Yoshi!


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: Scemilope] * 2
    #28276448 - 04/13/23 07:46 PM (9 months, 10 days ago)

Thank you guys!

I put a bunch of agar with exotics on them into parchment today. The two at the very bottom are mini BRF/verm cakes.



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InvisibleScemilope
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 2
    #28276560 - 04/13/23 08:55 PM (9 months, 10 days ago)

I have used bags of desiccant pellets to pull excessive condensation out of no-pour agar jars. I closed them up in a small tub for a while, worked great. Could be effective for drying agar and grain pouches too.



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Edited by Scemilope (04/13/23 09:02 PM)


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OfflinePandaskis
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Re: Drying agar and grains using parchment paper. [Re: Scemilope] * 2
    #28276569 - 04/13/23 09:00 PM (9 months, 10 days ago)

Quote:

Scemilope said:
I have used bags of desiccant pellets to pull excessive condensation out of no-pour jars. Could be effective for drying agar/grain pouches too.






Wait does that work? If so, thats genius, i hate the condensation in no pours...


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InvisibleScemilope
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Re: Drying agar and grains using parchment paper. [Re: Pandaskis] * 1
    #28276575 - 04/13/23 09:04 PM (9 months, 10 days ago)

For my jars with vented lids it worked a treat. Took about 4 days to clear the worst of it.


--------------------
This hobby has taught me many things. Most of those things are patience.
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Edited by Scemilope (04/13/23 09:05 PM)


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InvisiblePsillySeeEms
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 1
    #28333162 - 05/24/23 07:23 PM (8 months, 7 hours ago)

Have you used argon\nitrogen\bloxygen wine preserving gas to store other dried materials before? I'm just wondering what all it could be useful for in the mycology realm. I use it anytime I crack into my freezer full of nicotine bases, or even for paints/stains, and many other products that would otherwise skim over or oxidize if you just shut the lid. Even sprayed some into bottles of seeds, to see if they'll last for decades from now, and still be viable.. I would think that completely displacing the air with the gas would be even better long term than vacuum sealing. Both methods together would probably work best maybe?. The argon sinks, so if you seal the bags upright after pumping them up, there would only be a bit of gas left in the bags that doesn't get vacuumes out... I dunno, that would waste gas though..  just making sure the dried materials stay in the bottom of the bag, and not towards the top where any air would be should work fine. Think of the gas like invisible water, as if you can pour it right into a glass, and even tip it and the gas will spill over the edge (if you could see it).. Anyway, good stuff!


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: PsillySeeEms] * 2
    #28333230 - 05/24/23 07:59 PM (8 months, 7 hours ago)

Thank you PsilySeeEms! I did buy a bottle of wine preservative with the intention of storing dried grains in 5ml cyrovials and displacing the atmosphere. So far, I haven't tried it, I need to change that!


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OfflineKinoko314
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 2
    #28333849 - 05/25/23 09:06 AM (7 months, 30 days ago)

Quote:

PsillySeeEms said:
Have you used argon\nitrogen\bloxygen wine preserving gas to store other dried materials before? I'm just wondering what all it could be useful for in the mycology realm. I use it anytime I crack into my freezer full of nicotine bases, or even for paints/stains, and many other products that would otherwise skim over or oxidize if you just shut the lid. Even sprayed some into bottles of seeds, to see if they'll last for decades from now, and still be viable.. I would think that completely displacing the air with the gas would be even better long term than vacuum sealing. Both methods together would probably work best maybe?. The argon sinks, so if you seal the bags upright after pumping them up, there would only be a bit of gas left in the bags that doesn't get vacuumes out... I dunno, that would waste gas though..  just making sure the dried materials stay in the bottom of the bag, and not towards the top where any air would be should work fine. Think of the gas like invisible water, as if you can pour it right into a glass, and even tip it and the gas will spill over the edge (if you could see it).. Anyway, good stuff!




I bought some Bloxygen, and I was really disappointed with how little was actually in it.

It's so little they have to tell you that it will feel empty. Because it almost is.

It might be worth it if it's going to remain undisturbed for a long period of time, but I like the cheapness of vacuum sealing.

You're probably right about Bloxygen being the better solution though.


Edited by Kinoko314 (05/25/23 09:07 AM)


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InvisiblePsillySeeEms
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Re: Drying agar and grains using parchment paper. [Re: Kinoko314]
    #28333994 - 05/25/23 11:37 AM (7 months, 30 days ago)

Yeah I know how that is. Feels like a rip off in a can, because it pretty much is. Holding the empty feeling can goes against everything you ever knew that felt right, lol. It's not bad for what it was designed to do though, and as often as you would normally use it. The gas (some brands have different mixtures, but bloxygen is only pure argon I believe) is heavier than air, so even a short 1-2 second burst from the spray can into a half full 1 gallon paint can for example.. it will settle down over the paint, and create a barrier like layer, keeping the rest of the air above it from being in contact. No need to fill the whole can.

Say you wanted to fill a jar full of loose dried fruits, you would probably need a lot more gas to displace all the air, so that it's up over the materials after it settles. It would be costly to do if you were constantly opening it up, or not being very efficient about the way you use the spray cans, and the kinds of containers you pick.

Sometimes I'll even poke a syringe down through the lid\foil on bottles of liquids that I've purged with bloxygen, because I know it will stay inside the bottle and not be wasted as much. I'm careful not to blow or tip it to the side, then only use a small burst to fill back up for good measure. Every few months or so I do that. My e-liquid nic bases stay good for years, just like they went in!

I always wonder if I could just take one of my old co2 tanks down to the welding gas supply, and have them fill it up with pure argon... but I never call to find out..


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: PsillySeeEms] * 4
    #28339296 - 05/29/23 10:58 AM (7 months, 26 days ago)

An update,
from this thread above

https://www.shroomery.org/forums/showflat.php/Number/28276448#28276448

I had two agar pieces grow mold. They were both bisporus that had stalled on agar for over a month so I'm not surprised. It was interesting to see the mold grow through the parchment paper. Thankfully all the other agar seems to have dried fine.





I've been slowly working through my collection of genetics, backing them all up. After cleaning up on agar, I scrape across the fan of mycelium and put that in 2ml of distilled water. I also noc up a jar of milo, let it grow out then dry it using parchment paper. Usually it just takes a couple days. Some of the dried grains get vacuum sealed, some go into 5ml cyro vials then sprayed with wine preservative (argon). The rest will get used and shared.



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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 5
    #28406280 - 07/24/23 10:37 AM (6 months, 1 day ago)

Slowly working the way through my spore collection, with a few duplicates of the wild prints.



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Invisiblestockw

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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer]
    #28428631 - 08/10/23 03:37 PM (5 months, 15 days ago)

Only just come across this! Amazing & exactly what I've been looking for. Cheers Yoshi


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: stockw]
    #28428743 - 08/10/23 04:56 PM (5 months, 15 days ago)

Good luck StockW!


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 3
    #28624783 - 01/17/24 04:13 PM (10 days, 10 hours ago)

Just a few updates.

Some species of mushrooms don't seem to store well on dried grains. Cubes seem pretty reliable but other sp. like Subtropicalis and Zapotecorum need more testing.

The type of grain used may also make a difference. Grains like oat  wheat and rye may work better in the long-term than seeds like millet??

Backing up agar to cyrovials with dH2O seems to be the most secure storage method but dried grains are very easy to share.


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Invisibletrip.momma
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Re: Drying agar and grains using parchment paper. [Re: YoshiTrainer] * 2
    #28624806 - 01/17/24 04:28 PM (10 days, 9 hours ago)

I love this thread, thank you for sharing this Yoshi!!! The drying of the whole oat grains has me sooooooo interested.. I always have a jar or two tucked away that ends up almost drying out on its own.. same with agar plates. Currently have some 7 months old and the agar it self has gotten incredibly thin


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OfflineYoshiTrainer
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Re: Drying agar and grains using parchment paper. [Re: trip.momma] * 1
    #28625050 - 01/17/24 06:12 PM (10 days, 8 hours ago)

Thank you Trip.Momma, gooduck!


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