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Re: Official Copelandia/Panaeolus Thread [Re: LogicaL Chaos] * 1
    #27690052 -

It would definitely be worth a try and I would think it would work.


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* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27690068 -

IME contam prints are from not being properly dried or just an outdoor culture.

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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27690084 -

How long do you let your prints dry Rocky? What about spores (edit: swabs)? I have some swabs drying in front of the hood for a couple hours tonight but saw an old post from Hamloaf where he says he dries them 24 hours in front of the hood. I don’t want to leave mine on that long. With a SAB, I was drying 24 hours.


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* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Edited by Hindsight (03/10/22 10:15 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27690152 -

I let prints dry for 4 or 5 days before folding and packaging them and I've had good results just swabbing, putting them back into the sleeve and taping them shut. The wooden stick seems to do a good job of acting like a wick and drying them out after a week-ish


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I :heart: 5318008


NOT a virgin!

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Re: Official Copelandia/Panaeolus Thread [Re: bigfootscreepyuncl] * 1
    #27690197 -

People have made cakes with all kinds of stuff, it definitely works.


With prints I usually give them two days to dry, swabs I leave in front of the hood 8-12 hours. Seems like overkill but there’s also almost no humidity here so stuff dries real fast.


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LAGM2020

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Re: Official Copelandia/Panaeolus Thread [Re: A.k.a]
    #27690209 -

Good info thanks guys. I haven’t been drying as long as some of you. I haven’t been having any contamination issues with my prints or swabs, but if taking them directly to a cake and skipping agar, I just want them to be as absolutely clean as possible.


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* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #27690213 -

I agree with previous statements. I'm not sterile or graceful and I'm confident in my swabs/prints 99% of the time.
Even drying out of flow.

All that being said my wild prints are notoriously fucked. Same with all the wilds I've received, those are fun though!

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Re: Official Copelandia/Panaeolus Thread [Re: LogicaL Chaos]
    #27690271 -

Could somebody please give me an up to date link to the "cake" recipes that are good for growing Panaeolus species? Thanks!


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I'm a stranger here myself.

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus] * 2
    #27690310 -

MacMerdin's Simple Pan-Cakes
It's older but still holds true.


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Crack's Tek's and Shit


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Re: Official Copelandia/Panaeolus Thread [Re: Crackatoa]
    #27690347 -

Thanks very much for this, will definitely give it a try.

OP mentions that for grass loving species ground grass seed is used instead of BRF. I'm still trying to familiarize myself with this genus and am unsure which is which.

I presently have spores of P. cyanescens (Huasteca and BVI) and P. subtropicalis. Any additional tips and suggestions much appreciated. Have had not so great results so far on agar. I should probably switch to grain water agar and see if things turn around. In addition to contamination problems from the spore syringes (I wish more people would do prints) I get a lot of tomentose growth, so much so that I almost pitched out the last bunch of plates thinking it was all mold...way too much genetic diversity in these syringes, maybe?


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I'm a stranger here myself.

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus]
    #27690358 -

I'm really sorry to ask these questions again, but haven't received answers yet...so at the risk of inviting abuse, here goes:

When making pancakes, I'm assuming that normal pasteurization of the media is warranted, correct? If so, how long and at what temp? I have to start using something other than my sterilizer, it runs way too hot.

I'm still confused about how to inoculate the "pancakes". I've been trying to do everything on agar, but it seems that people are just injecting spores directly into the cakes. How do I use the mycelia from agar to inoculate these? Sorry if this is a stupid question...

Edited to use pasteurize instead of "sterilize", which I know isn't that good for these sorts of growing media.


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I'm a stranger here myself.

Edited by Hongosamongus (03/11/22 01:01 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus]
    #27690364 -

You could make an LI (Liquid Inoculate) from clean colonized agar and place it inside a clean syringe. An LI is clean mycelium mixed with clean water. Its what I use for Cubes. Works great.

Im not sure on the sterilization but Im pretty sure you pasteurize manure-based substrates. But u can sterilize them too in a PC.

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Re: Official Copelandia/Panaeolus Thread Questions Questions.. [Re: Hindsight]
    #27690368 -

Hindsight said:
Thanks Crack. Ratio on this one was around 1:2 - 1:3. I've been mostly eyeballing it lately and I range anywhere from 1:2-1:4. For MS grows I go for around a 2" depth and for clone grows I go for around 3-3.5" depth. The only reason for doing less in MS grows is that I don't want to waste sub on a non-fruiting or poorly fruiting culture. Manure is expensive and difficult for me to get for now so that's why I hate wasting it. I have a wheelbarrow sized pile outside aging and if it ends up working well, I may go 3" on everything. 3" depth corresponds to a 1:4 ratio with 1qt of spawn.


Hindsight, what would you say is the prevalence of those 'non-fruiting or poorly fruiting' cultures percentage-wise?

After about 2 months I keep getting spindly fruits that don't open their caps and abort, despite trying all sorts of different conditions and subs/hydration levels/etc. I just streaked new plates to see if my culture might be to blame.

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus] * 1
    #27690369 -

https://files.shroomery.org/attachments/8125811-pancyan.pdf
Much easier to navigate for me.

An organized tomentose is the best way to describe pan cyan myc.

P. Subtrop is not here and has it's own place.
https://www.shroomery.org/forums/showflat.php/Number/27070123

Syringes are multi spore and will need to be transferred for recognizable growth. Best to use a drop on a sterile swab or loop to streak.

Edit: you had more questions! Check that tek out and then sit on it a few days and then ask questions.

Edited by rockyfungus (03/11/22 12:55 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: LogicaL Chaos]
    #27690380 -

How are you mixing the mycelia on agar? I know you can spend a lot on Eberbach jars but I doubt anybody here thinks that's a good idea. Will a lab stirrer (I have a really crappy one, barely works) do the job?


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I'm a stranger here myself.

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Re: Official Copelandia/Panaeolus Thread [Re: LogicaL Chaos]
    #27690383 -

Sorry, I meant "pasteurize". My point about the sterilizer that I have is that it runs way too hot to pasteurize, so I need to look up some teks for doing bulk substrates, if that's the correct term.


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I'm a stranger here myself.

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus]
    #27690386 -

I just take a piece of colonized agar and put it inside a clean petri dish then mix with clean water using a clean tool like tweezers or the needle end of a new syringe. The mycelium practically dissolves into the water.

Yeah look up "pasteurizing manure substrates". Im sure you will find something.

I got some growth of my two agar dishes! Tiny mycelium :eyeball:

Plate 1:


Plate 2:


Took 4 days :cool2:

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus] * 2
    #27690389 -

Hongosamongus said:
How are you mixing the mycelia on agar? I know you can spend a lot on Eberbach jars but I doubt anybody here thinks that's a good idea. Will a lab stirrer (I have a really crappy one, barely works) do the job?


I don't mean to be a dick. But you obviously didn't read the tek I posted.

"My agar method goes as follows, shoot 6 ccs of sterile water onto a colonized agar plate. Using the needle tip, mix up the Mycelium and water and draw the slurry back into the syringe. No need to dig into the agar layer, just scrape the Mycelium off the surface of the agar . inoculate the containers with 6 cc of Mycelium water. They'll be colonized within 15 days and ready to fruit. "

You're going to need to figure out how to pasteurize with simple things like a thermometer and a pot of water. You may need to work on cubes first. I'm trying to save you wasted effort.

Edited by rockyfungus (03/11/22 01:16 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27690394 -

Figured out why I was confused - I think the vendor has mislabled whatever they sent, because it's listed as Paneolus subtropicalis (sic) and it was sent as a bonus...I'll have to clear it up with them. The link you sent me to is about a Psilocybe species that I doubt they have, as it seems to be relatively rare.

I guess the grass seed vs BRF for "grass loving species" isn't much of an issue after all, right? And for cyanescens of all strains BRF is fine as a component in the recipe for this species?


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I'm a stranger here myself.

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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus]
    #27690399 -

I'm tapping out. You're on your own or people that are patient can help.
Good night!

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