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SwabMarley
Twisted Metal Head



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Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 2
#27696365 - 03/15/22 01:47 PM (1 year, 10 months ago) |
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That’s impressive Hindsight! Nice work as usual!
I got some Pan Jambos to grain, looking okay I think 2 days after a shake…
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Moopers
Pan noob



Registered: 05/08/20
Posts: 525
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27696440 - 03/15/22 02:48 PM (1 year, 10 months ago) |
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Quote:
Myc Hunt said:
Quote:
coversall said:
Quote:
Moopers said: My transfer plates have viable colonies for all three pans I started: PHV, Jambo, Pan cyan. I only have the one fruiting chamber, so I can only get one on grain and growing soon. The others, if I use them to inoculate LC, will they keep longer?
I recall folks here saying don't inoculate using old pan plates. So, is LC a viable option to keep them stable until I can use them?
Could be, I've used an LC that was full col for a month or so and kept at room temp. It performed well!
Are we still under the general consensus that Pan make does okay when stored in the fridge? If so, will it keep like cube myc for many months?
Quote:
Adas said: Look into my journal, I've recently done an experiment (not quite what you're asking, but should resolve a lot of concerns).
Thanks for the help. I'll probably go to grain with one l, move to LC for the next best-looking cultures, and put the rest of the good-looking plates in the fridge. Fingers crossed.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Moopers] 1
#27696463 - 03/15/22 03:09 PM (1 year, 10 months ago) |
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Great work everyone!!!
I want to share with newcomers a tip that JCM came up with, just in case anyone missed it.
He places his long-term storage cultures inside an insulated lunch box or a small cooler, and puts that in a fridge. That helps stabilize the temperature anytime the door gets opened. The stable temperature helps prevent condensation from building up, but I think it might help the cultures stay alive as well.
He also uses a stand alone fridge that he keeps a little warmer than a typical refrigerator, so that probably helps a lot, and would be recommended if possible. If not, maybe look for the warmest spot in the fridge and try to use that location.
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Moopers
Pan noob



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Posts: 525
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27696489 - 03/15/22 03:27 PM (1 year, 10 months ago) |
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Quote:
ruawakeyet said: Great work everyone!!!
I want to share with newcomers a tip that JCM came up with, just in case anyone missed it.
He places his long-term storage cultures inside an insulated lunch box or a small cooler, and puts that in a fridge. That helps stabilize the temperature anytime the door gets opened. The stable temperature helps prevent condensation from building up, but I think it might help the cultures stay alive as well.
He also uses a stand alone fridge that he keeps a little warmer than a typical refrigerator, so that probably helps a lot, and would be recommended if possible. If not, maybe look for the warmest spot in the fridge and try to use that location.
Thanks, that's a good tip. Time to go thrifting!
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Hongosamongus
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
#27696574 - 03/15/22 04:33 PM (1 year, 10 months ago) |
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Several things discussed in the Pan cyan PDF that you posted that I don't quite understand. It says to treat the horse manure with a combo of bleach and lime. However, it's vague about how much lime to use, or what the desired pH is. Sorry to bother you, but could you let me know, please?
Thanks.
-------------------- I'm a stranger here myself.
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus] 1
#27696621 - 03/15/22 05:15 PM (1 year, 10 months ago) |
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Quote:
Hongosamongus said: Several things discussed in the Pan cyan PDF that you posted that I don't quite understand. It says to treat the horse manure with a combo of bleach and lime. However, it's vague about how much lime to use, or what the desired pH is. Sorry to bother you, but could you let me know, please?
Thanks.
I haven't heard of anyone using bleach these days to treat manure. Using lime is cold pasteurizing. Rockyfungus is the one you'd want to ask about lime pasteurization as he uses it regularly and could probably give you some great info on it. The vast majority of us are using heat pasteurization now. Heat to 140-160F for 2 to 12 hours, then let it age in a sealed bag for 3-7 days. I follow JOC's recommendation on this by loading my substrate mix (straw, verm, and manure) into a 2.5 gallon ziplock bag, put it in an Instapot, add some water to cover, then put on the keep-warm setting overnight. But there are plenty of lower-dollar pasteurization tek's available - just do a search. The pasteurization tek is the same regardless of the substrate material so don't worry about that aspect.
RE storage: I've switched to room temp storage in little vials of distilled water. I'll let you guys know how the pans like it after a few months of storage.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Hongosamongus] 1
#27696647 - 03/15/22 05:33 PM (1 year, 10 months ago) |
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Quote:
Hongosamongus said: Several things discussed in the Pan cyan PDF that you posted that I don't quite understand. It says to treat the horse manure with a combo of bleach and lime. However, it's vague about how much lime to use, or what the desired pH is. Sorry to bother you, but could you let me know, please?
Thanks.
I think this is outdated, or perhaps a method to try to quickly leach manure that is too fresh.
I tried it once, it all went bad on me and was the worst smelling manure substrate ever! I think the most important thing is to have field-aged manure, and you can skip all that bleach and lime.
Hindsight, thank you so much for reminding us about the room temp water storage. I keep forgetting about that, and can't wait to hear your results. I know other growers have used it with success.
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Bra
grow panaeolus, it's easy


Registered: 07/12/19
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#27696954 - 03/15/22 09:31 PM (1 year, 10 months ago) |
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Hey guys!
What is your favorite Panaeolus strain? And why?
What you can say about Pan Tropicalis? Is it worth it?
And what is the most visual Pan strain in your opinion?
Thanks
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#27697122 - 03/16/22 12:13 AM (1 year, 10 months ago) |
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Q: What is your favorite Panaeolus strain? And why?
A: I've only grown one, called PHV. I chose it because it's a hybrid that Jakeoncid bred, and it did not disappoint. I personally think it's one of the most attractive pans I've seen. The bisporus is really attractive as well, but are also notoriously more challenging to grow. The PHV is my recommendation for new pan growers, because if I can grow it, so can others. (FYI, if you are looking to get some spores, I strongly recommend prints over syringes.)
Q: What you can say about Pan Tropicalis? Is it worth it?
A: What do you mean by "Is it worth it?" Is it more expensive than other pan prints?
Q: And what is the most visual Pan strain in your opinion?
A: I think this is too subjective to get any solid answer. Within any variety or species, there will be genetic differences from one culture to another. Two cultures taken from the same print can exhibit completely different growth characteristics and potency. There is still some luck of the draw regarding what sectors are selected from agar. (Play the odds by transferring several sectors from a germ plate, transfer until clean, and start off with small trays of each culture until you find which ones perform the best.)
Long story short, there aren't really any loser species or varieties. (Except for pan cinctulus, which is known to be quite weak, but I don't know of anybody selling prints of it. It has been grown by growers here, but mainly just for looks I believe.)
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Official Copelandia/Panaeolus Thread [Re: Bra]
#27697199 - 03/16/22 03:01 AM (1 year, 10 months ago) |
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Quote:
Hongosamongus said: Several things discussed in the Pan cyan PDF that you posted that I don't quite understand. It says to treat the horse manure with a combo of bleach and lime. However, it's vague about how much lime to use, or what the desired pH is. Sorry to bother you, but could you let me know, please?
Thanks.
I'd give up on that pdf is seems to have a lot of old, out-dated info in.
Quote:
Bra said: Hey guys!
What is your favorite Panaeolus strain? And why?
What you can say about Pan Tropicalis? Is it worth it?
And what is the most visual Pan strain in your opinion?
Thanks
The one that fruits best in my conditions. Not sure about tropicalis, but grow it and see!
Personally I think set and setting have a bigger impact than strain on visuals, etc.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: Bra] 1
#27697200 - 03/16/22 03:02 AM (1 year, 10 months ago) |
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Quote:
Bra said: Hey guys!
What is your favorite Panaeolus strain? And why?
What you can say about Pan Tropicalis? Is it worth it?
And what is the most visual Pan strain in your opinion?
Thanks
Bunnell FL Cyans are my all time favourite. They are consistent, resilient and produce well.
All Pans are very visual. You won't be disappointed.
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myco.bay
Stranger
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] 1
#27697502 - 03/16/22 10:25 AM (1 year, 10 months ago) |
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I finally have some success with my pan cyan grow after a couple tries. The only thing that is throwing me off is that they look caramel colored. A lot of the pans I see here are like an off white color to me. Any Idea what could be going on? I'll post a picture later when I can.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
#27697518 - 03/16/22 10:39 AM (1 year, 10 months ago) |
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Congrats!!! What substrate is it on? There has been some evidence lately suggesting that substrate may have an effect on the color of the fruits. It could also be a genetic variable. Cultures taken from the same print can exhibit drastically different characteristics.
I'm anxious to see them!
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myco.bay
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 2
#27697709 - 03/16/22 01:40 PM (1 year, 10 months ago) |
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The substrate is the one that jakeoncid sells. There are some metabolites on the surface of the casing layer of that matters at all. Here is a picture.
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Hindsight
Mad Scientist


Registered: 01/24/21
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
#27697718 - 03/16/22 01:48 PM (1 year, 10 months ago) |
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What variety is that?
I haven't seen that cap color on any pans I have grown though. The brown staining on the mycelium overlay looks odd to me. I have never seen that either. I do see the metabolytes, which look a bit heavy to me.... but I see some brown staining that doesn't appear to be metabolytes though it is hard to tell from the photo quality. There is also a lot of mycelium on the surface - more than I'm used to seeing on a pan tray. The casing layer should cover the majority of the mycelium on the surface.
Did you pasteurize the sub? What about the casing layer? What are you using for casing, when did you apply it, and did the mycelium overrun it?
Edited by Hindsight (03/16/22 03:47 PM)
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Ambrose

Registered: 01/16/20
Posts: 518
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay] 1
#27697731 - 03/16/22 02:07 PM (1 year, 10 months ago) |
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Quote:
myco.bay said: The substrate is the one that jakeoncid sells. There are some metabolites on the surface of the casing layer of that matters at all. Here is a picture. 
Looks like Psilocybe subtropicalis(aka semperviva) to me. Nice grow
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V.L


Registered: 12/15/17
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Loc: In my pants
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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay] 2
#27697739 - 03/16/22 02:27 PM (1 year, 10 months ago) |
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Quote:
myco.bay said: The substrate is the one that jakeoncid sells. There are some metabolites on the surface of the casing layer of that matters at all. Here is a picture. 
Hi mycobay I saw in the exotic thread you were working zap & caeru on agar too maybe somethings was mislabeled or exchanged during your work.. they are not Pans but some nice exotic Psilocybes sp
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myco.bay
Stranger
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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
#27697753 - 03/16/22 02:45 PM (1 year, 10 months ago) |
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It's supposed to be peace river. I did pasteurize both the casing layer and sub. The casing layer is just cactus potting mix. It definitely could be a mislabel as I do have some other exotics in lc, including subtropicalis and it did seem to take 3 weeks which seems unusually long for pans. In any case, I'll start back from spore and probably send these out for testing to see what they pop back up as. I'll try to take a better photo when I get back home.
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Adas
Lonely Dreamer



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Re: Official Copelandia/Panaeolus Thread [Re: myco.bay]
#27697814 - 03/16/22 03:44 PM (1 year, 10 months ago) |
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Quote:
myco.bay said: The substrate is the one that jakeoncid sells. There are some metabolites on the surface of the casing layer of that matters at all. Here is a picture. 
This is not a Panaeolus. VERY nice though, whatever it is! Could absolutely be Caerulescens, as VL said. In which case, congratulations! These are not easy.
Edited by Adas (03/16/22 03:46 PM)
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smalltalk_canceled
Babnik


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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27697843 - 03/16/22 04:02 PM (1 year, 10 months ago) |
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Dont look like semps to me
-------------------- Willpower is the one true virtue
  
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