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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Cultivation General Discussion [Re: Screwup] * 1
    #28263716 -

No worries man, it's all good.

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Re: Cultivation General Discussion [Re: abductee] * 1
    #28263846 -

abductee said:
Ugh, I think I got trichoderma for the first time. My first flush in this mono.



I got to see my first trich today as well. A shoebox from a bacterial jar that just never fully colonized... I knew the jar wasn't great from the start, but figured what the hell. :shrug:


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LAGM 2024

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Re: Cultivation General Discussion [Re: SeventhMushroom] * 2
    #28263857 -

SeventhMushroom said:
abductee said:
Ugh, I think I got trichoderma for the first time. My first flush in this mono.



I got to see my first trich today as well. A shoebox from a bacterial jar that just never fully colonized... I knew the jar wasn't great from the start, but figured what the hell. :shrug:


Next time just cover the bacterial jar with coir, throw a Ziploc bag on top and let it fruit that way.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Cultivation General Discussion [Re: SirPsycho]
    #28265419 -

Hi everyone,

Is this grain ready for substrate or should I wait longer / couple of days more?


(all pix same jar)

This was 3 days ago, when I shook it:


This is now:

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Re: Cultivation General Discussion [Re: drVetker] * 1
    #28265443 -

drVetker said:
Hi everyone,

Is this grain ready for substrate or should I wait longer / couple of days more?


(all pix same jar)

This was 3 days ago, when I shook it:


This is now:



I would wait a couple days.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Cultivation General Discussion [Re: Way] * 1
    #28265475 -

Ok thank you sir

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Re: Cultivation General Discussion [Re: drVetker] * 1
    #28265957 -

Looks excellent tho. It can be disastrous to put to coir grain that isn't 100%. (Imagine rubbing an uncolonized grain up against unsterile coir.) You are in good shape because you shook when it was well past 50% colonized, so you knew it was thoroughly mixed with mycelia. Its nice to be on the safe side and go 12-24 hours after you see it at what appears to be 100%. (what if the internal conditions caused slightly slower colonization) right?


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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Re: Cultivation General Discussion [Re: tedoro] * 1
    #28266458 -

I've recently got success with hydrating grains in an Insta Pot (-like PC). 6l pot, 2l grains, 2l water. I've got success with brown rice - cooking time 8 minutes - a few months ago with a bottle grow. I've now started a few bottles with milo hydrated this way for 13 minutes. I've got a bag each of wheat, rye and barley and try these next. The Idea behind this is, first you save electricity (I should measure that...), second you keep all the nutrients from boiling grains inside your grow, one Alternative would be to hydrate coir with grain water _ONLY_FOR_BOTTLES_, don't spawn that. Or make grain water LC, but I don't have use for that much LC.

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Re: Cultivation General Discussion [Re: tedoro] * 1
    #28266594 -

tedoro said:
Looks excellent tho. It can be disastrous to put to coir grain that isn't 100%. (Imagine rubbing an uncolonized grain up against unsterile coir.) You are in good shape because you shook when it was well past 50% colonized, so you knew it was thoroughly mixed with mycelia. Its nice to be on the safe side and go 12-24 hours after you see it at what appears to be 100%. (what if the internal conditions caused slightly slower colonization) right?


Ok thank you sir. It'll be out for a couple more days then it's going to the fridge for a pause because I need to go away for a while and then after two weeks it's going to substrate so we'll see how it all goes in the end, but I am stoked that this is looking nice and healthy.
I prepd 3 jars, two are good and one didn't move an inch. Smell says bacterial probably, but I have way to little experience to determine why.
All of them prepd the same way but PCd separately  due to a small PC. Inoculated from same plate at the same time. So what the hell happened to that one between PCing and inoculating I have zero clue.

Edited by drVetker (04/07/23 11:29 AM)

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Re: Cultivation General Discussion [Re: drVetker] * 2
    #28268477 -

drVetker said:
tedoro said:
Looks excellent tho. It can be disastrous to put to coir grain that isn't 100%. (Imagine rubbing an uncolonized grain up against unsterile coir.) You are in good shape because you shook when it was well past 50% colonized, so you knew it was thoroughly mixed with mycelia. Its nice to be on the safe side and go 12-24 hours after you see it at what appears to be 100%. (what if the internal conditions caused slightly slower colonization) right?


Ok thank you sir. It'll be out for a couple more days then it's going to the fridge for a pause because I need to go away for a while and then after two weeks it's going to substrate so we'll see how it all goes in the end, but I am stoked that this is looking nice and healthy.
I prepd 3 jars, two are good and one didn't move an inch. Smell says bacterial probably, but I have way to little experience to determine why.
All of them prepd the same way but PCd separately  due to a small PC. Inoculated from same plate at the same time. So what the hell happened to that one between PCing and inoculating I have zero clue.


Just starting out, 2 outta 3 is a pretty good success. A lot of old timers here would likely question the 'sterile technique' as the cause of the bad jar. It takes some time to get the motions of inoculating down. There are hundreds of subtle ways one can do a slight, but wrong move and a jar goes bad. Watching videos of experts (not self-deemed) noc'ing up jars seems to always reveal something they do that we aren't.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC

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Re: Cultivation General Discussion [Re: tedoro] * 2
    #28269271 -

I’m hosting a contest if anyone is interested here


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Cultivation General Discussion [Re: Smellyhobbit] * 1
    #28269459 -

got my first trich contamination - game over? should I wait for more fruits? throw it away and burn it with fire? whats the common next step?


--------------------
P i c k l e    R i c k :dancer:



Pick Rick's Mushroom Chocolate

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Re: Cultivation General Discussion [Re: Pickle Rick] * 1
    #28269461 -

Yeah just toss it


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Cultivation General Discussion [Re: nektar61]
    #28269564 -

Hello,I was thinking if it could be the coir, but I noticed some new jars that had green growing in it before going to coir. I believe my grain  sterilization was done improperly on a couple batches because the PC going off was causing meltdowns. I turned it too low and it didn't get to proper stable heat.
I have a few plants in the house too, I wonder if that causes more chances of contams.
I use eco earth coco coir from a pet store. I been busy, but got some monos, dub tubs and a couple 20 litter bins going.
I ended up re sterilizing a run of grain and it looks super dark. Is it possible to over cook the grain? They are not mushy.

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Re: Cultivation General Discussion [Re: abductee]
    #28269626 -

abductee said:
Hello,I was thinking if it could be the coir, but I noticed some new jars that had green growing in it before going to coir. I believe my grain  sterilization was done improperly on a couple batches because the PC going off was causing meltdowns. I turned it too low and it didn't get to proper stable heat.
I have a few plants in the house too, I wonder if that causes more chances of contams.
I use eco earth coco coir from a pet store. I been busy, but got some monos, dub tubs and a couple 20 litter bins going.
I ended up re sterilizing a run of grain and it looks super dark. Is it possible to over cook the grain? They are not mushy.




how long is your PC cycle? did you vent your PC before starting your cycle?

I've used grain I sterilized for 8+hrs(accidentally) when I first got my 75x's, they were dark as fuck but still worked...

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Re: Cultivation General Discussion [Re: Leftfield420]
    #28271309 -

Thanks for the personal experience info 👊. I PC for 2 hrs for quart jars. I start the timer when it starts to move. I believe i used to do an hour and half with no issues, but I've been seeing two hr time frames since I've gotten back into it. What is venting?

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Re: Cultivation General Discussion [Re: abductee]
    #28271314 -

abductee said:
Thanks for the personal experience info 👊. I PC for 2 hrs for quart jars. I start the timer when it starts to move. I believe i used to do an hour and half with no issues, but I've been seeing two hr time frames since I've gotten back into it. What is venting?


Venting is allowing the initial air and steam mixture to escape so the entire PC is full of only steam and operates at the correct pressure. 

Start the PC with the weight off. When steam starts escaping, set a timer for about 15 minutes. After that timer is up, put weight on. When psi hits 15, start timer for sterilization.


--------------------

That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: Cultivation General Discussion [Re: Way]
    #28272171 -

question --> just opened a new 5kg brick of coir. it felt soft when I opened it and the coir is damp. is this safe to use? if so, how do I get it to field capacity, I presume it would need quite a bit less water


--------------------
P i c k l e    R i c k :dancer:



Pick Rick's Mushroom Chocolate

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Re: Cultivation General Discussion [Re: Pickle Rick] * 2
    #28272192 -

Uff, coir should actually be dry af. Damp coir could probably hold some contams, so I personally would feel safer if I would pasteurize it. To bring it to FC I would start with like maybe adding 3x the weight of it in Water, see how the FC is and if needed just add more hot water until its good and then pasteurizing it.

But I am no expert, it's just my opinion ^^


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
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Re: Cultivation General Discussion [Re: Shrimps] * 1
    #28272485 -

Weigh out 100g and throw it in the oven on 200 for a few hrs and weigh it again. You now know the exact % of hydration it sits at when it stops changing.

I like my subs around %70 water %30 coir/verm by weight


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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