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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28159700 -

Hindsight said:
Awesome sounds like you have some good steps. I swear by bleaching the tubs. I think it makes a real difference, especially when you have tubs that had trich blooms in them on previous grows. I have tested it, and if there are any spores left behind in the tub that miss your scrubbing with a scrubber and dish soap, they will germinate. Bleach is a great insurance policy, and cheap, and easy.

Agree on trying coir this time, instead of straw. I feel pans like straw better but I have had many wonderful grows on coir/manure. Bispos especially seem to like it.

Don't give up and keep on trying!



Why are minuscule amounts of spores left behind in the tubs different to spores that are getting in during spawn? Wouldn't all tubs contaminate then? Wouldn't be trace amounts of mold mycelium more likely to be responsible for repeated trich outbreak in the same tub?


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πŸ„PANAEOLUS IN MONOTUBSπŸ„
πŸ„OFFICIAL SEMILANCEATA THREADπŸ„



⭐Baba's Write Ups and Link Collection⭐

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28159744 -

Yes, mold fragments are way worse than just spores, which are all over anyway. I think the Trich can partially hide in micro-cracks in the plastic.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28159749 -

When I empty a tub that had a trich outbreak in it, there are large quantities of green spores that have attached themselves to the tub. If you are scrubbing the tub in a hurry, it can be easy to miss some areas. Add the fact that many tubs have latches and other areas that feature various ridges and indentations and these areas are easy to miss and extremely difficult to really scrub with a scrubber. But hit them with bleach spray and every spore in there gets deactivated, guaranteed. The spray gets into the nooks and crannies easily too.

The amount of trich spores floating around in the air has to be far less than the amount of trich spores that could be left behind in a tub, depending on how well the tub was scrubbed and how many places there are that you can't get to. My trich experiments have shown that a certain quantity of spores in your tub/substrate will result in a trich infestation - the mycelium what you are trying to grow won't fight it off. I haven't run any tests on how much trich spore it takes to overwhelm mycelium and cause a trich bloom, but so far I can say, if you can see anything green with your naked eye (meaning green trich spores), it's enough to cause a trich bloom. The argument could be made that the amount in the air is below the threshold required for a full blown infection (unless your air is thick with spores), theoretically.

TL;DR - I don't think the amount of spores left in a tub is actually minuscule. I have seen thick green liquid, big green chunks, etc. I also don't think people clean their tubs that well. I know I've been guilty of that in the past. Sometimes you get away with it. Sometimes you don't. Sometimes I'll rinse one but not scrub it and I'll toss it into the stack of tubs and then when I need a tub, I'll assume all of them in the stack were properly washed/scrubbed.

Edit - Adas beat me to it


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* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Edited by Hindsight (01/27/23 01:43 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28159773 -

Ah OK, when you said cleaned then I was assuming properly cleaned. I wouldn't argue that visible spore load could have an effect on sub health but I would also assume that a visible spore load will also contain fragments of trich mycelium. Anyway I am on team bleach after trich as well.


--------------------
πŸ„PANAEOLUS IN MONOTUBSπŸ„
πŸ„OFFICIAL SEMILANCEATA THREADπŸ„



⭐Baba's Write Ups and Link Collection⭐

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28159780 -

Yeah, kill it with fire. I like. Don't want to go down that road again xD


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28159805 -

Damn, I’m probably due for a mass bleaching of all my tubs.. honestly jars too. I hate using bleach, I know I can use gloves, but I got nightmares of cleaning butcher rooms and disintegrating my hands from bleach.. if only I was smarter back then perhaps I wouldn’t hold such disdain..

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28159806 -

And if you run out of bleach you can always just boil some water and pour that over the entire tub surface. Will kill everything instantly.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 5
    #28159813 -

Another cleaning PSA, don’t be like me. In a rush and not wanting to clean my coffee grinder I decided what could go wrong if I just ground my coffee with all the pan residue left over. I’m here to tell you unless you like waking up in the morning and wondering why the fuck your bugging out clean your grinder.. I swear it took me a solid 30mins before I remembered that I did that..

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28159815 -

Well, I did wash it out thoroughly with soap and cooking hot water from my shower for about 10 minutes. But I will bleach the shit out of it anyway, better save then sorry^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] * 2
    #28159820 -

kirkeng said:
Another cleaning PSA, don’t be like me. In a rush and not wanting to clean my coffee grinder I decided what could go wrong if I just ground my coffee with all the pan residue left over. I’m here to tell you unless you like waking up in the morning and wondering why the fuck your bugging out clean your grinder.. I swear it took me a solid 30mins before I remembered that I did that..


Well, at least you enjoyed some psyched coffee! :rofl: Hilarious how potent they are, aint it?


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 6
    #28159824 -

Yeah lol, I once swiped out the dust that got stuck on the grinder sides and lid with my finger and licked it off. 30 min later I was harvesting some cubes and looked at every single fruit thinking how beautiful they are. Took me ages to harvest.


--------------------
πŸ„PANAEOLUS IN MONOTUBSπŸ„
πŸ„OFFICIAL SEMILANCEATA THREADπŸ„



⭐Baba's Write Ups and Link Collection⭐

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28159829 -

It is enjoyable, I wish I was responsible enough to label it or something, reminded me of when I used to drink and in a drunken stupor I’d spike my morning coffee to fuck with my sober self.. I thought I was passed those days.. alas we push on.

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng] * 1
    #28159835 -

Bisporus is kinda hard to tell for me, it's so whispy. The Jar seems fully colonized and is also "one piece" in the Jar.

But kinda funky looking for me:







Any thoughts?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28159836 -

Looks like a perfectly healthy Bisp jar! :cheers:


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28159838 -

So I assume, my Nec-D senst it, and killed themselfes that I have room for new stuff maybe :shrug:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 8
    #28159844 -

Panaeolus Cyanescens



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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28159847 -

I trust adas, though I’ve never seen a jar that faint for bisporus, mine typically look like this,

Not saying it ain’t good though, I’m just surprised at how faint it is.

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Re: Official Copelandia/Panaeolus Thread [Re: kirkeng]
    #28159849 -

Yeah, could maybe have got a bit forgotten and was maybe like colonized already a couple of weeks ago^^

Edit: But yeah, it looks sketchy I agree^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28159851 -

Could it be because your grain is prepped on the dry side....kinda looks like it.


--------------------
πŸ„PANAEOLUS IN MONOTUBSπŸ„
πŸ„OFFICIAL SEMILANCEATA THREADπŸ„



⭐Baba's Write Ups and Link Collection⭐

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28159855 -

It just looks that way ;-) (It still has alot of weight) It started to looked like dryed out when it got fully colonized :shrug:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Shop: North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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