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InvisibleBaba Yaga
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The Official Semilanceata Thread * 28
    #27613283 - 01/09/22 06:22 PM (2 years, 18 days ago)

Official Semilanceata Cultivation Thread


I think it's time to pay some more attention to this species and giving it it's own place here in Mush Cult.

Psilocybe semilanceata, aka Liberty Cap, is one of the most wide spread psilocybe which means it is suitable to grow in a big portion of this planet.
The truth is though that there is hardly anyone trying to grow these and I ask myself why? Reading through the threads I found that lots of people are
under the impression that Libs are a very difficult to cultivate species and this might be true for indoor cultivation, but outdoors they are very easy
to grow. The key here is to keep it simple. Don't get hung up on old information that says they need rye grass seed or decaying grass roots to thrive. 
Cronicr for example said that they will grow just fine from grain spawned to coir and buried in a shady spot in the garden after full colonization.

So my point is, if you are already doing outdoor grows with wood lovers then I would highly recommend to switch it up a bit and giving some liberty caps
a chance when you are planning your next fall outdoor project but what ever you do, whether it's outdoors or indoors, please pop by and let us know how
it's going.

The photos below are from a grow in 2020 posted in the Mushroom Grow Logs and Pictures Forum plus a few from a grow in 2022.
Almost all the phenotypes you can see there are quite different from semilanceata fruits found in the wild. These fruits were so different that I had
substantial doubt that what I was growing was indeed Ps. semilanceata and not a similar psilocybe like fimetaria. To make sure that I wasn't growing
anything else I took a sample to get a gene sequence analysis done and the result came back positive for Ps. semilanceata.


Fist image is an alignment of my sample sequence with another semilanceata sequence in GeneBank which is showing
only one difference between the two. Second image is a phylogenetic tree showing the sample highlighted in green:





Ok, here are some photos to set the mood:
































:sun:
















Edited by Baba Yaga (04/02/23 05:17 AM)


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 7
    #27613285 - 01/09/22 06:23 PM (2 years, 18 days ago)

I'm glad I got this one finally on the road after members smalltalk-canceled and Land Trout have decided to get on the train to Libs Ville as well.

Most excited to see more grows of this species as the cultivated phenotypes are nothing like the wild specimens you can usually find
which makes some members skeptical that what has been grown so far is actually semilanceata genetics. Smalltalk has already send samples to
Alan Rockefeller for analysis and I will send samples to Alva Lab in Spain to get species confirmation, so fingers crossed that I will
get fruits this fall.
 

This years spawn and cakes are already on their way. I inoculated 20 oat jars last week with LC and I have good growth. Don't mind the
PF-substrate on top of the grain, I inoculated the jars with 2 month old LC and just wanted to make sure it recovers alright, if you are
interested in what I did then have a look here.



Almost 3 month ago I inoculated 16 cakes, I can't remember what the substrate is exactly as I made it a year ago and it was sitting in the
freezer since then but it does contain some h-manure and coir and I mixed in some old grass clippings and put wheat bran in.



Also made 10 jars with straw, h-manure, coir and whole meal flour. They are colonizing and consolidating for almost 4 month now.



A few have what appears to be knots or sclerotia but nothing else happened so far.



I cased a couple with coir and took them in and out of the fridge a few times but also nothing happening, I hope putting them outside
once it gets cold will kick off fruiting.



My fruiting strategy for my outdoor beds is the same as the last two years which is finding some good quality compost in bags and spawn
to that but after the fail last time I want to make sure that I will get fruits this year so I will pasteurize some of the compost to get rid
of competing organisms and use that to spawn to some flower pots cause the compost I used last year was too soggy and smelled bad,
probably not well composted and nothing grew. This year I will make sure to get some nice earthy smelling product.

I'm also prepping a patch by laying down grass clippings for the last couple of month. They are composting and I want to spawn some
grain to this and was also thinking of having some of this pasteurized for a few flower pots.



Got heaps of material for this season so I hope this will all colonize alright and give me some results.

Won't be long now before it's getting colder here, a couple more month and it will be time to spawn some libs. :awedance:


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OnlineLand TroutM
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 5
    #27613314 - 01/09/22 06:39 PM (2 years, 18 days ago)

Let’s do this


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: Land Trout] * 1
    #27613345 - 01/09/22 06:55 PM (2 years, 18 days ago)

Sweet! This will probably be a slow start as we are between seasons globally speaking but I hope some more folks will chime in over time
and blow this thread up.


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InvisibleMysticMycologist
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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 1
    #27613442 - 01/09/22 08:25 PM (2 years, 18 days ago)

I wonder if they could be grown outdoors in the Midwest, USDA zone 6a? Never tried liberty caps.‘I would love to try them as I hear they are unique.


--------------------
Two eyes to look, One eye to see.
Prying open my third eye


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OfflineHindsight
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Re: The Official Semilanceata Thread [Re: MysticMycologist] * 1
    #27613451 - 01/09/22 08:32 PM (2 years, 17 days ago)

:threadmonitor:


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OfflineMycoplex
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Re: The Official Semilanceata Thread [Re: Hindsight] * 1
    #27613487 - 01/09/22 09:21 PM (2 years, 17 days ago)

Useful thread Baba, will stick around here and check out how the thread evolves.


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: MysticMycologist] * 2
    #27613582 - 01/09/22 10:35 PM (2 years, 17 days ago)

Quote:

MysticMycologist said:
I wonder if they could be grown outdoors in the Midwest, USDA zone 6a? Never tried liberty caps.‘I would love to try them as I hear they are unique.




I would say yes if you time it right. Worth a try.


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OfflineAnglerfishM
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Re: The Official Semilanceata Thread [Re: MysticMycologist] * 1
    #27613879 - 01/10/22 08:51 AM (2 years, 17 days ago)

Quote:

MysticMycologist said:
I wonder if they could be grown outdoors in the Midwest, USDA zone 6a? Never tried liberty caps.‘I would love to try them as I hear they are unique.




I assume that if you get some healthy mycelium going, they can be fruited outdoors given that
the conditions are favourable for the time it takes for them to get into fruiting mode and develop pins.
I think high RH is the key here, temperatures too, but not as much, although they seem to need a burst
of cold to get going.


--------------------




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OfflineTweeq
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Re: The Official Semilanceata Thread [Re: Anglerfish] * 1
    #27614207 - 01/10/22 02:43 PM (2 years, 17 days ago)

Yes! An official Semilanceata thread. I'm in. Managed to finally clean up my wild clone on agar. Still plenty time to think up some different spots and strategies.


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Invisiblefungusul
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Re: The Official Semilanceata Thread [Re: Tweeq] * 1
    #27614539 - 01/10/22 09:47 PM (2 years, 16 days ago)

Great idea Baba. :takingnotes:


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OfflineLibhunter2021
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Re: The Official Semilanceata Thread [Re: fungusul] * 1
    #27614771 - 01/11/22 06:49 AM (2 years, 16 days ago)

Awesome thread!


There's a great grow on mycotopia I'll try to find if yous like, the guy grows them in a wine cellar nd has success managing his temps.

Amazing to see if the other grow on the hunting sub gets confirmation from AR.

I have prints of strong samples of libs but maybe not the cleanest I only took them as a momento of strong finds, maybe be good for agar work. I've a print of a specimen that was 0.25g dry it was one the biggest I found hunting this year I was gonna try and run it on agar. When I read workman's work on this he used an albino, I was curious how it would work with a print of a "strong" specimen as I assumed albinos would be weaker genetic?

Cook thread, great to see and maybe take part in. I've only learnt cubes but I can agar/grain/spawn/set it outside no problem. My nearest hunting spots are less than 10min walks.


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OfflineWaldfried
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Re: The Official Semilanceata Thread [Re: Libhunter2021] * 1
    #27614816 - 01/11/22 07:46 AM (2 years, 16 days ago)

Very strange phenotype indeed. Might give it a try if I come across some spores. Hadn't had any luck with finding some for the last years though. :shrug:


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Offlinewxorx
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Re: The Official Semilanceata Thread [Re: Waldfried] * 1
    #27614947 - 01/11/22 10:02 AM (2 years, 16 days ago)

Subscribing


--------------------
void **


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OfflineLibhunter2021
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Re: The Official Semilanceata Thread [Re: wxorx] * 1
    #27615118 - 01/11/22 12:35 PM (2 years, 16 days ago)

[i[/image]

Can't find the pic of the actual fruit but these are prints from a few a found at reasonable altitude mid to late August when it was still in the low 20s high teens temp wise. They were big, strong and early for that spot so I thought they'd be perfect and to me at that stage just a momento of first picks of last season. Hadn't rained much when I got those but clouds lie on the hillside so humidity micro climate.


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Offlineholofractal
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Re: The Official Semilanceata Thread [Re: Libhunter2021] * 5
    #27615147 - 01/11/22 01:20 PM (2 years, 16 days ago)

Hell yeah man!!!!!

I just got my shoebox started, birthed my 1qt grain spawn to 3qt of compost. Very rich and dark, smells wonderful, not wet or stinky. Cased it with some rye, although I should get something that grows a little shorter lol, as I've learned. It was the only organic non-coated with bullshit grass seed I could find on amazon, but perhaps i need to go to a garden center.



That's one of the favorite liberty cultures I ended up with. Quite a slow grower for sure, and that is both the fastest and also "nicest" mycelium I managed to culture.




Here is T0. Bonus bacteria from the NL, poor thing would get engulfed by mycelium haha.




The grain jar I did. Very wispy and thin, took some thinking to agree to when it was "done" as it did not wholly engulf the grains as other species do, so I gave it an extra 2 weeks before I used. The smell was wonderful, and I didn't note any other particular smell, just smelled like any other grain spawn.

So as of yesterday, just waiting for my shoebox grass to sprout. I do plan on doing some more outdoor patches, but I got plenty of time before then, and even though in the pnw its not that cold, there is still risk of freeze  and neither I nor the fungi want to freeze their ass off, so I'll wait another month or two. I got a very thick liberty cap LC waiting to be used, so pretty much all ready for the seasons work.

I should also note that stuntzii and baeocystis mycelium looked quite similar to semilanceata mycelium, which I found interesting.


--------------------
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I am open to questions about wood lovers, I don't know everything, but if you like my posts and have a question, feel free to ask in a PM
I do a lot of indoor experiments. I, one day, WILL figure out a surefire method for indoor woodlovers. Nothing is impossible.

Indoor Woodlover experimentation Journal
Indoor woodlover information - condensed
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OfflineMartain
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Re: The Official Semilanceata Thread [Re: holofractal] * 3
    #27615231 - 01/11/22 02:25 PM (2 years, 16 days ago)

It might be of interest to consider the mychorrhizal associations with certain grassland species where these are found.

Isolating cultures from the natural soils where these grow and incorporating the resident biota could prove fruitful. It may be that there are other fungi or bacteria in association as well, noone has ever studied it conclusively.


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InvisibleBaba Yaga
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Re: The Official Semilanceata Thread [Re: holofractal] * 2
    #27615245 - 01/11/22 02:34 PM (2 years, 16 days ago)

Quote:

holofractal said:


That's one of the favorite liberty cultures I ended up with. Quite a slow grower for sure, and that is both the fastest and also "nicest" mycelium I managed to culture.





That is a really nice plate!

Great to see this is sparking some interest :rockon:


Just re-posting some culture images for reference:



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Re: The Official Semilanceata Thread [Re: Baba Yaga] * 3
    #27615303 - 01/11/22 03:33 PM (2 years, 16 days ago)

Finally there's an official Semi thread!

Today I've buried some spawn into a flowerpot with a nice patch of Festuca glauca. The grass is not only beautiful, it should provide great conditions for mushrooms.


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Offlinesmalltalk_canceled
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Re: The Official Semilanceata Thread [Re: Adas] * 2
    #27615337 - 01/11/22 04:04 PM (2 years, 16 days ago)

I think complexity is a trap here, go for simple subs.

Allow me to say why.

Here are some of my *thoughts* on the subject.

My idea of this species is that both grass and manure is unnecessary to accomplish fruiting. If my attempt is verified, a very simple sub will be documented, without either.

Later we may still find that either helps or promote something relevant, so adding either may still be preferable.


But on the way?

Makin our steps too advanced slows down the process as a whole. Perhaps slowing down the most important repetition, and adding attempts that are less meaningful.

Doing so steals from the agar work, diversity of genetics, adds pasteurization and contam vectors because of added complexity

I believe with this species, we are looking for genetics that respond to the what we normally do. And we need to fish industrially, not with a single line

We don't have a indoor method from A to finish, but we are not reliant on that.

We can:
- run alot of different germination/cultures, looking for outliers, agar pins, mature agar fruits
- run numerous but small subs
- we can dig them down outside in time for season
- we can colonize then indoors, and then take them to colder temperatures, this seems to have triggered pinning with the unverified semi in my thread


--------------------
Willpower is the one true virtue



Edited by smalltalk_canceled (01/11/22 04:08 PM)


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