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Shrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Southerner]
#28167424 - 02/01/23 11:23 AM (11 months, 21 days ago) |
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Yeah,... First time with this strain. So I just think there are alot of different strains in there which makes it look kinda funky^^
I could be mistaking though xD
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination š
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Hindsight
Mad Scientist


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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 4
#28167441 - 02/01/23 11:33 AM (11 months, 21 days ago) |
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It should look like this:

You could be right about what you are seeing being due to different cultures all climbing over one another, but it could also be due to contamination. You are rolling the dice if you send it and that means wasted time and materials. I would transfer until you get something more organized. I'm not in the camp of taking transfers to the extreme. I can't remember the last time I got to T3 and even T2 is only like 5-10% of my plates - I almost always send T1. It's ok if you see sectoring, but it should look more clean and organized.
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Southerner
Stranger

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] 1
#28167447 - 02/01/23 11:36 AM (11 months, 21 days ago) |
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I would think that even a large amount of strains should be able to work together and form a somewhat organized colony. Also, there seems to be some bluing in the center of the dish. I have seen it on agar before but not until the plate was 100% and Iām also doubtful that culture was clean. Iām not very experienced with pans though so hopefully someone can chime in on that.
Hindsight beat me to it.
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Shrimps
Traveler


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Re: Official Copelandia/Panaeolus Thread [Re: Southerner]
#28167457 - 02/01/23 11:43 AM (11 months, 21 days ago) |
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Well I'm rolling the dice on this one yes. But I have a backup dish ;-) where I will make a new transfer from to get a cleaner culture ^^
Tbh, I also just wanted to get some petri dishes empty because I ran out xD
The bispo thought looks perfectly fine to me on the other hand. So there I am quite sure I'm not rolling the dice ^^ We will see.
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination š
š“ š° š¼ š² š» šø š½ š¶ š
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Scotay
The Mad Mush Guy

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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28169435 - 02/02/23 04:25 PM (11 months, 20 days ago) |
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Quote:
ruawakeyet said: I have so many questions about your process.
Are you using a SAB or a flowhood?
What's your process for sterilizing your water and syringe / needle?
What do you use for your agar plates? (Jars; Pastyplates; Condiment Cups; Petri Dishes?)
What kind of grain jar lids are you using?
How do you inoculate your jars?
Have you tried skipping the water and using direct agar wedge to grain inoculation?
What is your sterilization technique for your grain?
I'm pretty sterile. I use a SAB. Alcohol with a little soapy water. Gloves. I use boiled water. Flame sterilize my needle with a shot glass alcohol burn outside the SAB. I use condiment cups for plates. Cheap and easy. I PC my grain for a couple hours. I think I'm actually going to try not adding water and drop straight agar from the plate instead of making a slurry. I just use silicone injection Port for my jars to squirt the slurry in. I appreciate you asking.... Sometimes it's just good to review... I'm trying to cover the bases. Haha. Happy Thursday ya panhead you. šāļø
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! šāļø
Edited by Scotay (02/02/23 04:44 PM)
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examine
Learner


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Re: Official Copelandia/Panaeolus Thread [Re: kondu] 14
#28169768 - 02/02/23 07:52 PM (11 months, 19 days ago) |
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I'm pretty new here, and so I'm partially celebrating my first really successful pan cyan flush. But I think I have something to add to the conversation because I haven't seen many fruiting photos from this variety.
This is my first flush from a TTBVI print I got from GordoTEK. I used his method for growing pans in a Martha tent on a straw/manure/verm sub with a peat/verm/lime casing layer. I harvested at this point because I was concerned about the caps with spots particularly along one edge of the tray and wanted to grab what I could if that was from a contam that might spread to the other fruits.

Edited by examine (02/02/23 07:55 PM)
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Seeker604


Registered: 11/30/20
Posts: 331
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Re: Official Copelandia/Panaeolus Thread [Re: examine] 1
#28169783 - 02/02/23 07:56 PM (11 months, 19 days ago) |
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Quote:
examine said: This is my first flush from a TTBVI print I got from GordoTEK.


Nice job!
Peace & Light,
Seeker604
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Shrimps
Traveler


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Re: Official Copelandia/Panaeolus Thread [Re: Seeker604] 7
#28170246 - 02/03/23 04:03 AM (11 months, 19 days ago) |
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I spawned this bispo (that funky dried looking Jar) on the 29th, so 5 days ago and it's just speeding through the substrade (1:2 ratio).

Think I am going to case it this evening or tomorrow morning.
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination š
š“ š° š¼ š² š» šø š½ š¶ š
š
š° šæ
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Baba Yaga
ā„ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: examine]
#28170763 - 02/03/23 01:10 PM (11 months, 19 days ago) |
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Quote:
examine said:

Nice coverage.
Only spots that I can see are from caps dying and bruising.
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28170852 - 02/03/23 02:23 PM (11 months, 19 days ago) |
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Hey Baba
Try looking at Examine's pic in his user gallary. What it's happen on that?
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Baba Yaga
ā„ coir grower

Registered: 09/13/20
Posts: 3,958
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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
#28170883 - 02/03/23 02:48 PM (11 months, 19 days ago) |
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Ah ok, this photo was hiding in examine's user gallery.

This is a different tray though I think so not sure if they are even referring to this.
Anyway, that is definitely contamination. No clue what it is and everyone's process is different so one can only guess when it's getting in. For me this type of contam is mostly culture based and I go back to spores or germ plate.
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THE MYCOSMITH
Weekend mycologist


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28170948 - 02/03/23 03:49 PM (11 months, 19 days ago) |
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Your right Baba. I read his post again an saw that it's was the spots on the caps that concerned him. And as you just told those craks comes from drying.
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ruawakeyet

Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 1
#28170965 - 02/03/23 03:59 PM (11 months, 19 days ago) |
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Quote:
Baba Yaga said: Ah ok, this photo was hiding in examine's user gallery.

This is a different tray though I think so not sure if they are even referring to this.
Anyway, that is definitely contamination. No clue what it is and everyone's process is different so one can only guess when it's getting in. For me this type of contam is mostly culture based and I go back to spores or germ plate.
This was from Examine's post 24 days ago. It was trich.
https://www.shroomery.org/forums/showflat.php/Number/28132607
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 12
#28171168 - 02/03/23 06:24 PM (11 months, 18 days ago) |
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Cross post from BabyYaga's mono thread:
Tubs are much slower than the tent, this took 8 days from first pins. I harvested a bit early but loved the uniformity of the fruits! About 6 inches in height.
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Baba Yaga
ā„ coir grower

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Re: Official Copelandia/Panaeolus Thread [Re: cooleko]
#28171208 - 02/03/23 07:03 PM (11 months, 18 days ago) |
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Noice!
How much faster is the tent?
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YoshiTrainer
Onion tied to belt



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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#28171294 - 02/03/23 08:07 PM (11 months, 18 days ago) |
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That is sweet Cooleko!
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Scotay
The Mad Mush Guy

Registered: 05/14/22
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Re: Official Copelandia/Panaeolus Thread [Re: YoshiTrainer]
#28171379 - 02/03/23 10:04 PM (11 months, 18 days ago) |
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Happy Friday/Saturday all you panheads!!! I hope your week has been good. Hope your weekend is full of pans to pick!! Peace and chicken grease to y'all!! šāļø
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! šāļø
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] 2
#28171427 - 02/03/23 11:06 PM (11 months, 18 days ago) |
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Quote:
Baba Yaga said: Noice!
How much faster is the tent?
3-4 days in the tent! However, I was gone the first 5 days of this tub and once I started misting it heavily twice a day it really took off, so I wonder if it was waiting for me to excessively water it.
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SingularFusion


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Re: Official Copelandia/Panaeolus Thread [Re: cooleko] 4
#28172072 - 02/04/23 01:20 PM (11 months, 18 days ago) |
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Bispo at five days after casing

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Shrimps
Traveler


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Re: Official Copelandia/Panaeolus Thread [Re: SingularFusion] 1
#28172094 - 02/04/23 01:33 PM (11 months, 18 days ago) |
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which casing
-------------------- I am not crazy, I prefer the term mentally hillarious. Agar - The way to go! Clean spawn checklist Proper Surface Moisture Recognizing and dealing with contamination š
š“ š° š¼ š² š» šø š½ š¶ š
š
š° šæ
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