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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28162445 - 01/29/23 07:16 AM (1 year, 9 hours ago)

Ah thanks for the info and clearing up my mistake! Didn't want to spread false info, sorry!!! I was assuming the little bit air in there would actually suffice for the mycelium until fully colonized :3 But I will gap the lid a little. Thank you!

Well, I had hydrogen peroxide just laying around. But I will look up if I finde such a spray with sodium hypochlorite. Shouldn't be expensive either ;-)


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28162460 - 01/29/23 07:36 AM (1 year, 8 hours ago)

No worries, sorry if I wasn't clear enough.

Here is the bleach spray I use.... I mean, I use whatever the store I'm at has in stock but where I live, you can ALWAYS find some brand of bleach spray and it's really all the same thing. Just check that active ingredient:
https://www.acehardware.com/departments/home-and-decor/cleaning-and-disinfectants/disinfectants/1386028

If you want to save money, buy plain bleach in a bottle like this, then dilute it to 2-3% and put in a sprayer bottle. Most sprayer bottles won't work for very long once you've put bleach through them though - the seal in most sprayers isn't designed for bleach, but you can find a kind that will withstand it if you look.
https://www.acehardware.com/departments/home-and-decor/laundry-care/bleaches/1005957


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OfflineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 4
    #28162463 - 01/29/23 07:42 AM (1 year, 8 hours ago)



Pan bispo dropped into rye and straw bottles Jan 25th.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] * 5
    #28162480 - 01/29/23 08:00 AM (1 year, 8 hours ago)

So, lets see if I can get at least some good genetics from my last Nec-D tray I've lost to the green devil. Cross your fingers please :laugh:



--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 4
    #28162512 - 01/29/23 08:36 AM (1 year, 7 hours ago)



More cyan huasteca going to a pint of millet today.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinemikeybox
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #28162537 - 01/29/23 09:06 AM (1 year, 7 hours ago)

I'm trying to fruit my pan cambo Goliath in cake tins in a Martha.

My Martha is home-made and allows me to program in a low humidity threshold and a high humidity threshold, and it will cycle back and forth between those 2 humidity levels every 2 hours.  I can set the desired humidity level and it will maintain it very accurately.

So, can someone recommend what is the ideal humidity range for pinning, and what is the ideal humidity range for fruit development?

Thanks in advance!


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: mikeybox] * 3
    #28162927 - 01/29/23 01:49 PM (1 year, 2 hours ago)

I don't suggest using humidity sensors and controllers. Cycle timers work better. 15-30 minute cycles are good. Some go longer, some go shorter. The reason humidity controllers don't work well is that unless you have a $500+ one, they are too slow to update and get water-logged when run in high humidity environments like a Martha tent, causing them to give false readings like 99% RH when it's nowhere near that.

I have a write-up here on how I do it: https://www.shroomery.org/forums/showflat.php/Number/27993602


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OfflineSirPsycho
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #28162928 - 01/29/23 01:51 PM (1 year, 2 hours ago)



A couple of cyan plates post transfer. The first plate is the same that I posted earlier. Second plate had a weird looking surface around the edges, even open I can't tell if it's bacteria or just weird drying of the agar.


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:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] * 8
    #28163026 - 01/29/23 02:39 PM (1 year, 1 hour ago)

NecD









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Mystery Mushroom


Edited by mushroom_gnome (01/29/23 02:42 PM)


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28163196 - 01/29/23 04:36 PM (11 months, 23 days ago)

Quote:

Baba Yaga said:
Quote:

FriedEgg said:
does anyone have any idea why my nec d fruits look derpy? they are bending over and curving all around. i don't think that's blotch on the caps. i think my fogger has been spraying water on the caps. also i'm pretty sure i'm getting enough FAE. my jambos grow in the same FC and they don't look like this at all.







Will be interesting to see if they will contaminate before the second flush. I am still not sure what causes this. It's either contam or genetics IMO.



i just harvested it today, misted it, and now the second flush is already knotting up. i don't think it's stressed because of a contam. maybe i accidentally cloned a mutant :shrug:


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] * 1
    #28163199 - 01/29/23 04:38 PM (11 months, 23 days ago)

Quote:

ekabpils said:
Quote:

FriedEgg said:
does anyone have any idea why my nec d fruits look derpy? they are bending over and curving all around. i don't think that's blotch on the caps. i think my fogger has been spraying water on the caps. also i'm pretty sure i'm getting enough FAE. my jambos grow in the same FC and they don't look like this at all.





It is OK for NecD isolated cultures. It is hybrid with unstable genetics and some cultures give unexpected results ) I had the same one for several cultures.



so you're saying MY nec d spores have unstable genetics? or ALL nec d spores are unstable because nec d is a hybrid?



beautiful photos mushroom_gnome :thumbup:


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Invisibleekabpils
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg] * 4
    #28163914 - 01/30/23 07:40 AM (11 months, 23 days ago)

I played a lot with different isolated cultures of NecD taken from agar. And there is a lot of veriaty of how the mashroomas are looked like. Some were like classic pans - tall and thin. Others short and thick.

For about 10 other pans I growed I didn't see the same veriaty - all the cultures are more of less the same.

Example of the most thick necD culture I had:


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] * 2
    #28164031 - 01/30/23 09:14 AM (11 months, 23 days ago)

I noticed a lot of variability in NecD as well. Including the white ones in my signature. I am still trying to reproduce them but every time I pull it off, it's like 3 weak looking fruits for the whole shoebox. Nothing like the MS grow produced. Mostly given up on it.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 6
    #28164053 - 01/30/23 09:29 AM (11 months, 23 days ago)

Some pic updates:


Mystery SA still slowly coming along. Definitely not as vigorous as all the other pans I've grown. It will be interesting to see what my HWFP-supplemented, non-cased tray does. It is in the tent now at 100% colonization. Pics to follow:


The accidental bispo tray progress (coir-only). Top half looking like maybe 1-2 more days to maturity. This was the tray I was testing two different casing recipes with. There is a clear preference for one recipe.


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InvisibleMycolorado
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28164055 - 01/30/23 09:32 AM (11 months, 23 days ago)

Wow…what was the casing difference?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Mycolorado] * 3
    #28164175 - 01/30/23 11:00 AM (11 months, 23 days ago)

The front casing uses pickling lime, a substantial amount of gypsum, and has a pH of IIRC 8.5.

The back casing uses hydrated lime, no gypsum, and has a pH of IIRC 9.5.

I believe pickling lime and hydrated lime should be the same thing. My hydrated lime doesn't have magnesium in it. I'm not a chemist though so not totally sure these are exactly the same. I might do another test .;

Don't take any of this as science just yet because this is my first round of casing testing between these two formulas and there is a small but real chance I got the labels reversed. As in, I applied one casing to one half, another to another half, then had to rotate the tub a time or two for some other reason and then sat there looking at it going, "oh.... wait...." like that scene in Idiocracy where he's at the "doctor" and has to stick the one sensor in his mouth, the other in his ear, and the other in his butt, and the doctor says "oh, no, sorry that's wrong, here... put this one in your mouth, this one in your ear, and this one in your butt". Give me one more round of tests where I don't make that same mistake again and it will then be conclusive.

I've been experimenting with higher than normal casing pH as a way to prevent overlay and colonization of the casing. I've been running all my casing like that for 9+ months and it has worked well. I am only now starting to case with more "standard" pH levels just to see how it compares to my high pH casing which I know works well on most everything I have grown. It seems there might be some varieties/species that don't like it but that's not conclusive yet.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28164180 - 01/30/23 11:02 AM (11 months, 23 days ago)

Just out of curiosity. How do you messure your PH? Just squeez the soil for a couple of drops on a PH stripe?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28164185 - 01/30/23 11:04 AM (11 months, 23 days ago)

I've tried that, and have tried sticking the strip in the moist casing and squeezing. Both work and seem to produce the same result. Liquid test kits are more accurate. But based on the amount of lime I'm adding for each recipe and the results I'm seeing on the test strips, I am inclined to believe it's pretty accurate - within 0.5 anyway.


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28164188 - 01/30/23 11:05 AM (11 months, 23 days ago)

Yeah the strips are fine. I was just eyeballing the caco3 xD


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I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28164211 - 01/30/23 11:18 AM (11 months, 23 days ago)

Idiocracy…classic. :lol:

Look forward to seeing what you turn up!


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