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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28151764 - 01/22/23 09:23 AM (1 year, 7 days ago)

Quote:

Hindsight said:
making spore syringes, dipping my loop into them so I pick up more spores when scraping a spore print





Hey Hindsight, In regards to your 2ml vials, I'm new to taking prints but I've been making some prints of my pan cyan estero and I have been leaving them on foil.  I usually scrape the print with a loop when I want to start a new plate.

If I wanted to store in distilled, would I scrape spores from the prints into one of these 2ml vials with scalpel? Or would I use a loop, flame sterilize, dip in distilled, scrape print and put back into new vial?

Basically, how do I get a bunch of spores from my prints into the vials? Thanks

Nice BVI tray too. 


Edited by Timestop413 (01/22/23 09:32 AM)


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Invisibleruawakeyet
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 1
    #28151780 - 01/22/23 09:37 AM (1 year, 7 days ago)

I don't think you want to store pan spores in water. Store them on the print.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 3
    #28151782 - 01/22/23 09:38 AM (1 year, 7 days ago)

You would scrape the spores from the prints into the vials. Another way to do it would be to pull the water from the vial into a syringe, raise the edges or concave your foil print a bit, squirt some water onto the print, then use a sterile and cooled scalpel to mix the spores into the water, then suck that back up with the syringe and squirt that liquid back into the vial.

I'm not a big fan of spore syringes because spore prints on foil are very rarely clean and if you have any bacteria on there, even endospores that will withstand drying, it will ruin your whole spore solution. It is far better to just keep the print dry on foil and scrape it with your loop as needed and swipe a plate.

Keep in mind that when we are talking about long term storage in a vial here, we are talking about storing mycelium, not spores. I'm not even sure how long spore solution will last at room temp or stored in a fridge because I just never go down that road.


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OfflineYoshiTrainer
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28151802 - 01/22/23 09:49 AM (1 year, 7 days ago)

Thank you guys for the links and the wonderful explanation Hindsight.


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28151925 - 01/22/23 10:39 AM (1 year, 7 days ago)

Quote:

Hindsight said:
I'm not a big fan of spore syringes because spore prints on foil are very rarely clean and if you have any bacteria on there, even endospores that will withstand drying, it will ruin your whole spore solution. It is far better to just keep the print dry on foil and scrape it with your loop as needed and swipe a plate.

Keep in mind that when we are talking about long term storage in a vial here, we are talking about storing mycelium, not spores.




Ok great, thank you for the explanation Hindsight. 

I've also wondered about this; despite taking the prints and letting them dry in front of a flow hood, I assumed, like you said, there would be some contaminants on the prints, whether they fall from the caps or where ever.

Why is it that these contaminants don't seem to grow when swiping a plate from a print?  I understand that in a liquid solution, bacteria will spread much easier. Are agar plates not conducive for whatever contaminants are on the print to grow out?  I usually do a transfer or two but sometimes my first swiped plates look pretty clean (not saying they ARE clean but I was surprised at how well a plate grew out from some prints).  Does the bacteria/mold die out when dried out on a print?


Edited by Timestop413 (01/22/23 10:43 AM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 1
    #28152372 - 01/22/23 03:08 PM (1 year, 7 days ago)

Many molds and bacterial cells have much shorter lifespan in dry conditions than fungal spores. And bacterial endospores usually aren't super abundant on prints, and a few bacterial colonies here and there usually don't spell doom to an entire plate.


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28152434 - 01/22/23 03:48 PM (1 year, 7 days ago)

To add to what Adas said, if you DO have some contamination on a print and you make a syringe/vial out of that print, you are guaranteed to get the contamination in the syringe/vial and it will be well distributed in the liquid. If you took the same print and rubbed your loop on a part of it, the chances the part you rubbed will have contamination that then sticks to the loop and makes it over to your plate is reduced, and even once it gets on your plate, it will likely be isolated to the area it ended up, while you still have spores across a broader area. So what you end up with on the plate is some areas of contamination and some areas of clean growth - the latter you can take transfers from. If you have a contaminated spore syringe, you will likely only get a bunch of bacteria to grow on the plate - no mycelium, depending on how bad it is, but usually they are pretty bad.


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Invisiblealaskappalachian
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 3
    #28152450 - 01/22/23 04:02 PM (1 year, 7 days ago)



Only pans I've been able to grow here in this cave have been bisporus in these little kimchi containers, and had a little nook open up so couldn't resist when I cleaned a couple
out. Finally will have space to run some tubs of various pans soon but until then  at least I'll have something to much on.  They get a better spot by the woodstove than I do :stoned:  Wish these didn't take so long to fruit.  Maybe just genetics. :shrug:


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: alaskappalachian] * 4
    #28152466 - 01/22/23 04:17 PM (1 year, 7 days ago)

Hey, if I was told I could only grow one pan, it would be Bispos so that's a good problem to have!


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28152507 - 01/22/23 04:57 PM (1 year, 7 days ago)

Quote:

Hindsight said:
That's some serious fog FriedEgg - You running a 6-disc? I'm running a 3. It has worked well for my standard size martha tent but I might up to a 6 disc at some point.

Pic tax - My latest BVI tray. This is first flush from a clone culture I took about a year or more ago, stored in distilled water vial at room temp. It is feeling very good to finally get some fruiting going on after that gnat infestation.





12 disc
:yeahthatsright:

nice BVI :thumbup:


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InvisibleSoutherner
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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
    #28152519 - 01/22/23 05:05 PM (1 year, 7 days ago)

:lmafo: that hit just right. Thanks FriedEgg.


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OfflineBlueAndOrange
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28152763 - 01/22/23 07:49 PM (1 year, 6 days ago)

Quote:

Hindsight said:
Hey, if I was told I could only grow one pan, it would be Bispos so that's a good problem to have!




Why would this be your choice? The way they cultivate or the effects taking them? Or something else?


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange]
    #28152824 - 01/22/23 08:33 PM (1 year, 6 days ago)

Quote:

BlueAndOrange said:
Quote:

Hindsight said:
Hey, if I was told I could only grow one pan, it would be Bispos so that's a good problem to have!




Why would this be your choice? The way they cultivate or the effects taking them? Or something else?




I am curious as well, whats good with Bisporus?

I was listening to an interview recently where someone said their favorite pan was pan cam/indonesia (Is this pan cambodginiensis?) as well as PHV.  Have you guys noticed different potencies across pan varieties?


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Timestop413] * 7
    #28153116 - 01/23/23 02:51 AM (1 year, 6 days ago)

Ladys and Gentleman! May I proudly present, the first (for me) ever pan pin to show me some love :laugh:

Both of my Nec-D trays are showing now slowly a couple of tiny pins forming :3 I think it's probably a bit slower since the room temps varry from 20°C to 22°C.



But it's fine, I am happy :smile:

Edit: Picture is bad but it's just so tiny, I couldn't get a better one yet^^


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Edited by Shrimps (01/23/23 03:14 AM)


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28153133 - 01/23/23 03:27 AM (1 year, 6 days ago)

:amusedapplause:


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OfflineTimestop413
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28153191 - 01/23/23 05:03 AM (1 year, 6 days ago)

Quote:

Shrimps said:
Ladys and Gentleman! May I proudly present, the first (for me) ever pan pin to show me some love :laugh:






:happyclaps:


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: BlueAndOrange] * 5
    #28153269 - 01/23/23 06:37 AM (1 year, 6 days ago)

Congrats Shrimps! I remember how happy I was when I saw my first Pan pin. You are well on your way.

Quote:

BlueAndOrange said:
Quote:

Hindsight said:
Hey, if I was told I could only grow one pan, it would be Bispos so that's a good problem to have!




Why would this be your choice? The way they cultivate or the effects taking them? Or something else?




More potent, better visuals, and less prone to bad trips. You can have a bad trip on them, like anything, but they seem to have more euphoria than other pans I have grown (about 5 different pan cyan varieties). The visuals in particular seem to be much stronger than cyans.


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Offlinehao
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28153296 - 01/23/23 06:57 AM (1 year, 6 days ago)

Quote:

Shrimps said:
Ladys and Gentleman! May I proudly present, the first (for me) ever pan pin to show me some love :laugh:

Both of my Nec-D trays are showing now slowly a couple of tiny pins forming :3 I think it's probably a bit slower since the room temps varry from 20°C to 22°C.



But it's fine, I am happy :smile:

Edit: Picture is bad but it's just so tiny, I couldn't get a better one yet^^





Congrat shrimp
The emotion seeing the first pin is no words !

Good work !


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: hao] * 2
    #28153871 - 01/23/23 01:43 PM (1 year, 6 days ago)

Shrimps, congrats, and welcome to the Pan cult! :skol:

I second the notion that Bisporus is superior. I find it maybe a bit weaker than Bunnell Cyans, but it certainly makes up for it with the quality of effects. And the high vibrations in my body on the comeup always get me.

They supposedly have high Norbaeocystin, so that could be part of it.


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 3
    #28154151 - 01/23/23 04:04 PM (1 year, 6 days ago)



:awedance:


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