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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28158461 -

Dont have a pic but it started bruising blue. Some parts had pools of water, others got a bit too dry. Uneven, damaging conditions. Not good.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28158490 -

I noticed similar surfaces with bruising when fruiting uncased after full colonization. They were still doing not too bad considering. FAE from the start and running them "dry" till first knots appear worked out much better though.


--------------------
๐Ÿ„PANAEOLUS IN MONOTUBS๐Ÿ„
๐Ÿ„OFFICIAL SEMILANCEATA THREAD๐Ÿ„



โญBaba's Write Ups and Link Collectionโญ

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28158873 -

Baba Yaga said:
Fingers crossed. Were there lots more side pins? Any knots forming on the bottom?


Those fruits don't seem to be originating from below the surface of the substrate. I see no side pins or knots on bottom. I think it's just that it REALLY doesn't want casing, and there are tiny little spots around the very edge of the tub that the casing didn't cover, and that is where it chose to throw up fruits. I run my casing pH rather high so it could also be that this species or variety doesn't like it. All the other pans I have grown do though.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 1
    #28158916 -

Just had a read through the last pages of zifozonke's OP about these. So interesting and curious to find out what makes this species thrive.


Alan Rockefeller said:
Here is a Panaeolus phylogenetic tree I made, with the sequence of this mystery Panaeolus highlighted with a red dot.



I see that it's close to a few Panaeolus bisporus sequences - I wonder if it has two spored basidia.

I agree that it's either a new species or a species that was described but not sequenced.



--------------------
๐Ÿ„PANAEOLUS IN MONOTUBS๐Ÿ„
๐Ÿ„OFFICIAL SEMILANCEATA THREAD๐Ÿ„



โญBaba's Write Ups and Link Collectionโญ

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 6
    #28158932 -

Went outside and had a look at the compost pile. Some subs are pushing out some nice big caps although it hasn't rained here for the last 1.5 weeks with warm weather and I only covered the pile with a few sticks. The caps are cracked but big and nicely bell shaped. This is why I am thinking about using far more FAE in future grows and lowering the water supply as well.



It started to rain today which makes me curious about what is going to happen with those discarded subs. Hoping for something good.


This year a wild stramonium turned up at the compost pile which casts a bit of shadow on the spot.



--------------------
๐Ÿ„PANAEOLUS IN MONOTUBS๐Ÿ„
๐Ÿ„OFFICIAL SEMILANCEATA THREAD๐Ÿ„



โญBaba's Write Ups and Link Collectionโญ

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 4
    #28159000 -

Here's something interesting:

Had some stalled shoeboxes that colonized fantastic, but didn't show any signs of fruiting for about 2 weeks. The surface was matted and drying up. I went to toss them, then as a last ditch effort made a random decision to mix up the top half of the substrate (straw/manure). Basically I "tilled" it with a fork. I soaked it, then...
4 days later- pins galore!

I think the disturbance contributed to the mycelium going "oh shit" and mass producing fruits. Also, I was having a hard time achieving the necessary humidity/fae ratio with such a low tech method (plain ol' shoebox). I noticed that on some "pan-cake" jars the pins liked to form down in little crevasses and nooks where, I think, the humidity stayed high. I'm pretty sure the flat surface of the tamped down casing layer in the shoebox caused too low a humidity for pins to form, so when I '"tilled" the substrate I left it all fluffed up and rough with lots of little nooks for humidity to get trapped. I'm pretty sure this is a significant contributor to the pins forming in this very low tech method. Something to play more with perhaps.









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Mystery Mushroom

Edited by mushroom_gnome (01/26/23 10:34 PM)

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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: mushroom_gnome]
    #28159049 -

Post deleted by Phaedra

Reason for deletion: X

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Re: Official Copelandia/Panaeolus Thread [Re: Phaedra]
    #28159229 -

Happy Friday you panheads!! I hope your week has been good and your weekend better! I shook a jar yesterday and it bruised up. I'll probably put it to bulk in a couple days. I've never seen mycelium bruise up like this. One step closer.....
Hurry up and wait. ๐Ÿ˜ŽโœŒ๏ธ


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Just chillin like a Villain and talking to like minded individuals!!
             


              Mush Love!!
                ๐Ÿ˜ŽโœŒ๏ธ

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Re: Official Copelandia/Panaeolus Thread [Re: Phaedra] * 1
    #28159301 -

Hey Hindsight

Its somehow nice (in a sad way) to see you having trouble with the Mystery sa. to. So i dont feel thats its me that do something wrong. V.L. Had god luck with his hemp pan-cakes maybe try some 25/35/40 h-poo/straw/hemp chips.
If you do not have been looking in Zifozonke's posts look at the last post(post Nr. 11)in this thread.. A pan growers wet dream.

https://www.shroomery.org/forums/showflat.php/Number/28054189#28054189

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #28159572 -

Fuck that shit man....

My second nec-d tray seems also to have trich...

Argh I am so discouraged right now... :frown: should I maybe take such a tiny pin and try to clone it? Or better start over from print?

So the casing material I used, I have still got some left in the bag, and there it doesnt show any sign of contamination. But what I did, was I misted the Casing after I applied it. So maybe it also could have gotten in that way. Ah I don't know... Every step I took feeld correct and everything worked out perfectly until now. Don't really now where it went wrong :frown:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28159575 -

Shrimps said:
Fuck that shit man....

My second nec-d tray seems also to have trich...

Argh I am so discouraged right now... :frown: should I maybe take such a tiny pin and try to clone it? Or better start over from print?

So the casing material I used, I have still got some left in the bag, and there it doesnt show any sign of contamination. But what I did, was I misted the Casing after I applied it. So maybe it also could have gotten in that way. Ah I don't know... Every step I took feeld correct and everything worked out perfectly until now. Don't really now where it went wrong :frown:


Either way you should join Bottle Gang. Not a lot of opportunities for trich with that


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Official Copelandia/Panaeolus Thread [Re: SirPsycho] * 1
    #28159578 -

Yeah hard for me to get those bottles you use^^

And I am way too nice person to join any criminal gang or so. I don't even look scary :frown:

No I think my problem was, I was thinking I could rock the pans easily and thought too highly of me. Now I fell from my horse and I'm butthurt.

Just going to start over again. But first I want to feel a bit longer butthurt and sorry for myself.

Edit: at least I can spot trich now in a split second^^ so something good came from it at least


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28159582 -

Shrimps said:
Edit: at least I can spot trich now in a split second^^ so something good came from it at least


This is this key. Every situation can be a learning experience that brings us one step closer to understanding.

Are you using LC?


--------------------
Mystery Mushroom

Edited by mushroom_gnome (01/27/23 11:50 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28159583 -

Yeah Sir, I've looked for these (or similar) wide mouth bottles far and wide, and it's impossible to come by. Best I have found is normal wide-mouth canning jars.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #28159600 -

No, I am dropping Agar wedges into grain ;-)

Edit: But would it be "save" or a "good Idea" to make a clone from a tiny pin before tossing my last pan tray? Or should I just start over from print again?


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28159626 -

If you think you can get a clone I would do that- the fact that it fruited at all is not insignificant. Id start some new spore plates as well to have two avenues going simultaneously. This will allow you to compare the clone to the new strain and narrow down environmental vs. genetic variation.


--------------------
Mystery Mushroom

Edited by mushroom_gnome (01/27/23 12:17 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 2
    #28159649 -

THE MYCOSMITH said:
Hey Hindsight

Its somehow nice (in a sad way) to see you having trouble with the Mystery sa. to. So i dont feel thats its me that do something wrong. V.L. Had god luck with his hemp pan-cakes maybe try some 25/35/40 h-poo/straw/hemp chips.
If you do not have been looking in Zifozonke's posts look at the last post(post Nr. 11)in this thread.. A pan growers wet dream.

https://www.shroomery.org/forums/showflat.php/Number/28054189#28054189


Yeah VL turned me on to shredded hemp chips. I have some and have used them quite successfully. I will try them in a future substrate batch. I just spawned a Mystery SA to a batch of normal pan sub but with HWFP mixed in so hoping that will do well.

@Shrimps, here is my advice on trich avoidance, end to end. I can't recall what your process is now so if some of this doesn't apply to you, disregard those portions. Take from this what you can and leave the rest:

1. Ensure your straw is very golden in color. I have seen straw that is more like hay - has some green in it. That won't work. Too much nitrogen. You might consider trying coir/manure instead of straw/manure. It works well but needs manure. Another option is shredded hemp + manure (https://www.amazon.com/Hemp-Bedding-Expands-Reduces-Chicken/dp/B08XYBD51V). Ensure your manure is aged properly. Manure chunks from out in a field that have dried out are ideal. Manure gathered from a stable that's been pissed on repeatedly and is still wet and green is too hot. If the latter is all you have, soak it in a 5 gallon bucket of fresh water overnight, drain/strain, put back in the bucket with a fresh 5 gallons of water and repeat that for 3-7 days. Then spread it out on a tarp or whatever and let it dry out. Then use it. My subs are 1:1:1 ratios of manure, verm, and then either coir, straw, or shredded hemp.
2. However you pasteurize, try not to let any part of the substrate get over 170F, and make sure you get to 160F core temp for 2 hours, as measured by a thermometer. I don't like the stove-top method because it often gets the outer layers too hot. It's not the end of the world but it can sterilize the outer layers which kills off the beneficial bacteria that help fight trich; you can mitigate this by allowing your pasteurized sub to age 7 days after pasteurization, theoretically, but I prefer to keep all parts of the sub below 170F at all times. I used to use the instant pot pasteurization method but once I got a bluetooth thermometer and checked it - I noticed the IP was only getting to like 130-140 and even then that took a LONG time. You also need to ensure your sub is moistened to field capacity during pasteurization to ensure proper pasteurization. Don't do it dry.
3. When spawning, ensure you use a bleach-based cleaning spray on your tub and lids. Let it sit 5 mins and rinse then dry with clean paper towels. Any residual trich spores in your tub WILL germinate when you put your sub in them. Also bleach whatever container your mix your prepared sub + grain spawn in, prior to using it. It's best to bleach these things right before you use them as the longer you let them sit, the more trich spores from your room air will land in them. Mix with gloves and clean arms. Get them in your tub, tamp down VERY lightly (I don't like to press my pan subs down hardly at all - just enough to get a moderately flat top surface), then put the lid on immediately and keep it on. I run my pans at a 1:2 to 1:3 spawn ratio but if you are having trich issues, go with lower ratios like 1:1-1:2. This ensures fastest colonization. Ensure you use a top layer of sub to cover your grains. I let colonization take place at 70-78F. Again, lid stays on. Don't open to peek.
4. Consider changing rooms, both for prep, and/or for colonization/growing, if you think maybe you have a high trich spore load in your house.
5. If you are using a casing layer, ensure it is non nutritious. I know a lot of people like cactus soil but it does have fertilizer in it. I prefer peat+verm 50/50 casing recipe and add enough hydrated lime to get it to 8.5-9.0 pH. Peat has a low pH and trich likes that. Ensure you use pickling lime or hydrated lime. There are other limes with magnesium added that you should not use. I use Hi-Yield brand Hydrated Lime. I use 2-3 teaspoons of lime per quart of 50/50 verm/peat mix. You should pasteurize your casing just like your sub - at field capacity.
6. Another note on pasteurization - I like to do it in ziploc bags, with as much air removed as possible, and do not open the ziploc bag until you are ready to use. Keep it closed! I like to use the sub or casing 3-7 days after pasteurization. If longer than that, repasteurize it.

Hope this helps.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28159678 -

Thanks alot hindsight!

I actually did everything like we once talked over PM. So I actually followed are the steps you mentioned above.
The straw has great quality, golden an cracker dry and no green it it. The manure I used is actually composted cow manure. Which is hot composted so actually also already really clean. I also pasteurized them (oven pasteurization) but I got fieldcapacity with warm water, so the coir temp was already 140F and put it in the preheated oven for about 4 hours.

I also use big Ziplockbag for the pasteurization and keep them closed until I need them. I let it sit for about a week before I used it. I still have a bag of the casing (50:50 peat/verm with 1tbs lime per L) I used laying here and it's still clean and doesn't show any form of contamination.

I didn't use bleachwater though to clean the tub and also use bleach in the water for inside of the tub. Maybe that's something good to start with :smile: Thanks for thank tip!

Now I was thinking of leaving the straw out and just work with composted manure and coco coir and look how that works for me. Just want to cut away on the possible contam risks. Also going to a 1:2 ration of spawn/sub for faster colonization.

Going to take some small pins from the sub in a ziplock bag and clone them later. Going to toss it out now and clean everything with bleach water.

Maybe I also just have too much going on at the same time and was distracted somehow... At least I have some good spores to continue working with. Just need a small break from this disappointment ^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28159683 -

Awesome sounds like you have some good steps. I swear by bleaching the tubs. I think it makes a real difference, especially when you have tubs that had trich blooms in them on previous grows. I have tested it, and if there are any spores left behind in the tub that miss your scrubbing with a scrubber and dish soap, they will germinate. Bleach is a great insurance policy, and cheap, and easy.

Agree on trying coir this time, instead of straw. I feel pans like straw better but I have had many wonderful grows on coir/manure. Bispos especially seem to like it.

Don't give up and keep on trying!


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28159695 -

For sure I won't give up :smile: You're pictures were the ones that inspired me in the first place, and then baba and kirkend with their great monos <3 Just need to get everything right. At least I got pins. It showed me that it's actually not that hard. Just need some tweaking here and there^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Boomr Bag   Mushroom-Hut Mono Tub Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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