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xVadisx
MountainsDogsAndFungi

Registered: 09/03/21
Posts: 1,537
Loc: Secret World HQ
Last seen: 1 year, 12 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27517951 - 10/25/21 06:57 PM (4 years, 10 months ago)

That's awesome. So many options! Thank you :smile:

:smile:

.

We will see if it stays clean and wait a little while while I form a plan (a2g most likely, lc will be shortly down the line for me but still down the line).

edit: I'll probably take a transfer or two as well :smile:

:smile:

. I also forgot to add that I swirled the pins in a pint jar with a cap of bleach (even dropped the smaller one on the FLOOR of the SAB fucketh me and picked it up again dipped it and off we went)

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Thanks to everyone for sharing what they know :smile:

:smile:

. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

Edited by xVadisx (10/25/21 07:05 PM)

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rockyfungus
dirty

Registered: 03/01/21
Posts: 1,097
Loc: Front range
Last seen: 2 months, 16 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: xVadisx]
#27518759 - 10/26/21 11:47 AM (4 years, 10 months ago)

Not sure what to make of these. Transferred off a wild cube pin from germ plate. All 3 plates show these linear growths.

Sorry PP5 hard to photo

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multifractal
Student

Registered: 05/08/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

[Re: rockyfungus]
#27519482 - 10/26/21 09:19 PM (4 years, 10 months ago)

From what I can tell from that photo they look good.

A clone from a beautiful phenotype showing some life:

And a solid T1:

Edited by multifractal (10/27/21 12:21 AM)

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Nigel Stephens

Registered: 02/21/20
Posts: 103

Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]
#27519825 - 10/27/21 07:58 AM (4 years, 10 months ago)

I streaked some agar plates with spores from a print 3 days ago in a SAB, then bagged the plates 2 at a time in new ziploc bags inside the SAB.

The plates are not wrapped, and are stored at 26C upside down to stop condensation on the agar.

So far, 5 of the 6 plates are showing the faintest signs of growth in the vicinity of the spores but too early to tell (to my laymans eye) whether it is myc or mould. It reaches out like myc so I don't think its bacteria anyway.

Question to you fine folks:

With unwrapped but bagged plates, how frequently are you comfortable handling them? I'm confident the bags are sealed, but mindful that I will eventually need to remove the plates from the bags to do xfers. So far, I have not handled the bags with gloves or used ISO. Should I?

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0ptiquest
Stranger

Registered: 07/05/20
Posts: 512
Last seen: 6 days, 11 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nigel Stephens]
#27519840 - 10/27/21 08:13 AM (4 years, 10 months ago)

Why dont you wrap them, clingwrap is cheap and works great.

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Nigel Stephens

Registered: 02/21/20
Posts: 103

Re: AGAR ENVY! (Anything and All things agar!)

[Re: 0ptiquest]
#27519858 - 10/27/21 08:21 AM (4 years, 10 months ago)

I have before, - well using gardeners grafting tape rather than clingwrap, but honestly found I was quite clumsy inside the SAB doing that. Knowing that the ziplocs are sterile out the packet is quite reassuring, though I do acknowledge some obvious downsides. I guess this is an experiment, will wrap any xfers if the germ plates make it that far

Is parafilm like Marmite? I actually hate it but know that many love it

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Failboat
Free man

Registered: 02/01/18
Posts: 8,741
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Nigel Stephens]

1
#27519868 - 10/27/21 08:35 AM (4 years, 10 months ago)

Cling wrap is best IMO. I was all like but parafilm stretches, but ya know it brwaks too.

Need to get me a pipe cutter.

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Never give up, never surrender.

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

2
#27519870 - 10/27/21 08:37 AM (4 years, 10 months ago)

I'm a big fan of the cling wrap

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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27520248 - 10/27/21 01:50 PM (4 years, 10 months ago)

Team cling wrap for the win. I've been using a bread knife to cut mine. I started out using a box cutter but I ended up with a lot of snaggy edges.

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rockyfungus
dirty

Registered: 03/01/21
Posts: 1,097
Loc: Front range
Last seen: 2 months, 16 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]
#27520253 - 10/27/21 01:54 PM (4 years, 10 months ago)

multifractal said:

From what I can tell from that photo they look good.

Thanks guess I'm not used to this manure/grain water. More sectoring then I'm used to. LC time

Going to leave these in a cold basement trying to clone for colder resistant fruits. If that works.

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,220
Loc: Gaming the system
Last seen: 17 hours, 12 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: rockyfungus]
#27520317 - 10/27/21 02:38 PM (4 years, 10 months ago)

Does the knife work better?

You definitely have to be careful with a box cutter. I’m pretty good at it now but I’ve had a few rolls that were super irritating cuz there’s a jagged piece that would snag and randomly cut the cling off mid wrap.

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Doctor Mario
🍄🍄🍄🍄🍄

Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27520367 - 10/27/21 03:22 PM (4 years, 10 months ago)

I really like the bread knife. The serrations make it more of a saw. You still have to be careful but I can cut a roll in about 5 seconds.

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Failboat
Free man

Registered: 02/01/18
Posts: 8,741
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Doctor Mario]
#27520428 - 10/27/21 04:25 PM (4 years, 10 months ago)

:nohellno:

--------------------
Never give up, never surrender.

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Atomsplit

Registered: 01/16/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Failboat]
#27520459 - 10/27/21 04:52 PM (4 years, 10 months ago)

Almost every kitchen benefits with a cleaver around. Bulletproof
Very useful when navigating through meats with bones and of course slicing through cling wrap rolls :crazy2:

:crazy2:

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.

Edited by Atomsplit (10/27/21 04:55 PM)

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Baba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,761
Loc: Trial 'n' Error Boot Camp

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27520499 - 10/27/21 05:29 PM (4 years, 10 months ago)

I am using a ratchet lopper. Just put a piece of corrugated cardboard under the cling roll so it doesn't get mangled by the notched side.
It helps as well if the blade is clean.

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aNewBeginning

Registered: 06/26/21
Posts: 708

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Baba Yaga]

2
#27520585 - 10/27/21 06:48 PM (4 years, 10 months ago)

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Tormato
Chairman of the Bored

Registered: 07/01/17
Posts: 6,147
Last seen: 13 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27520692 - 10/27/21 08:25 PM (4 years, 10 months ago)

aNewBeginning said:

Bruh!! Nice!!

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Big_Dub
I'm just some guy

Registered: 01/12/11
Posts: 3,643
Loc: Los Angeles
Last seen: 5 days, 20 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]

1
#27520706 - 10/27/21 08:30 PM (4 years, 10 months ago)

I 100% support cling wrap. So cheap and easy.

Never had a problem using even the cheap dollar store version.

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Atomsplit

Registered: 01/16/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]
#27520715 - 10/27/21 08:38 PM (4 years, 10 months ago)

:whathesaid:

cling wrap is sold everywhere and easy to work with.
I've tried parafilm once and found it a pita.
Maybe I had old stock?

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.

Edited by Atomsplit (10/27/21 08:47 PM)

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27520736 - 10/27/21 08:58 PM (4 years, 10 months ago)

I prefer cling because it's ubiquitous, but I think that people use parafilm incorrectly, I followed RR' example when I first started and that method is incorrect.

This is how to properly use parafilm:

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