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OfflineFluxburn
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Registered: 10/23/04
Posts: 2,216
Loc: Oakland, CA, USA Flag
Last seen: 12 years, 17 days
cheapest glass petri dishes, agar, dextrose, etc
    #3777169 - 02/14/05 11:01 AM (17 years, 9 months ago)

someone help me with my new obession, agar. So where is the best place to get all this stuff, ebay has cheap ass pyrex pertis for sure. I see the sporeworks has dextrose and mycoweb has some premade potato dextrose agar... I know you can make too.


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ABSTRACT ART (Mine) http://nathanbelomy.com


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Offlineazurescens
member
Registered: 02/17/04
Posts: 717
Last seen: 14 years, 5 months
Re: cheapest glass petri dishes, agar, dextrose, etc [Re: Fluxburn]
    #3777173 - 02/14/05 11:02 AM (17 years, 9 months ago)

cheapest way to go is to by the dishes and make your own.....it's very easy!!!!!


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Invisibleohmatic
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: cheapest glass petri dishes, agar, dextrose, etc [Re: azurescens]
    #3777177 - 02/14/05 11:04 AM (17 years, 9 months ago)

word - check the link in my sig for the beer agar project,
you can make a badass agar with supplies from your home :smile:

peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibleagar
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Registered: 11/21/04
Posts: 9,056
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Re: cheapest glass petri dishes, agar, dextrose, etc [Re: Fluxburn]
    #3777183 - 02/14/05 11:05 AM (17 years, 9 months ago)

Dry dextrose & light dry malt from home brew beer supply store (look in local phone book), agar from oreintal markets.

? pint Canning Jars & (Fool Proof) Potato Agar
? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.
? When ready to innoc (in glove box or flow/hood), remove lid, innoc & replace lid.
? Remove storage tape & replace with millipore surgical tape


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Invisibleohmatic
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: cheapest glass petri dishes, agar, dextrose, etc [Re: agar]
    #3777187 - 02/14/05 11:07 AM (17 years, 9 months ago)

Quote:

agar said:
Dry dextrose & light dry malt from home brew beer supply store (look in local phone book), agar from oreintal markets.

? pint Canning Jars & (Fool Proof) Potato Agar
? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the  agar.  (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.
? When ready to innoc (in glove box or flow/hood), remove lid, innoc & replace lid.
? Remove storage tape & replace with millipore surgical tape




ehehe thats funny .. take a look at my beer agar project and look at what you just posted agar =)
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: cheapest glass petri dishes, agar, dextrose, etc [Re: ohmatic]
    #3777193 - 02/14/05 11:11 AM (17 years, 9 months ago)

Grin..... :mushroom2:.........fun stuff isn't it. :grin: :wink:


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OfflineRoudypiper
newbie
Registered: 07/01/04
Posts: 103
Last seen: 16 years, 7 months
Re: cheapest glass petri dishes, agar, dextrose, etc [Re: agar]
    #3777667 - 02/14/05 04:46 PM (17 years, 9 months ago)

would the size 1 baby food jars work good for making agar???


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OfflineCyber
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Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 1 year, 9 months
Re: cheapest glass petri dishes, agar, dextrose, etc [Re: agar]
    #3777684 - 02/14/05 04:55 PM (17 years, 9 months ago)

Quote:

agar said:
Dry dextrose & light dry malt from home brew beer supply store (look in local phone book), agar from oreintal markets.

? pint Canning Jars & (Fool Proof) Potato Agar
? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.
? When ready to innoc (in glove box or flow/hood), remove lid, innoc & replace lid.
? Remove storage tape & replace with millipore surgical tape




When I make mine I do the following

#1) I add food coloring to ID the different agar's Red for PDA, Blue for MDA, etc.

#2) I put it in 1 pint jars for storage. once it is made I put it in pint jars, put the lids on correctly, and Pressure Cook them. In essence I can it and seal it. The stuff will keep for ever that way.

#3) when I am ready to use it I PC the jar (Turning it back to a liquid and unsealing the jar) then pour plates in the glove box.

You can get petties of e-bay for cheep, I traded prints for mine on here.



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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: cheapest glass petri dishes, agar, dextrose, etc [Re: Roudypiper]
    #3777690 - 02/14/05 04:57 PM (17 years, 9 months ago)

Quote:

Roudypiper said:
would the size 1 baby food jars work good for making agar???




You could...but they are sorta on the small side.


Keep checking Ebay...and snipe some glass petri dishes at the last few seconds of the auction.

Pleezr and I spent several weeks buying up all the glass petri dishes off Ebay...a few years back.
We got some hella great deals.

wide mouth 1/2 pints are a great way to start agar work cheap.

Good luck!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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