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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #27517951 -

That's awesome. So many options! Thank you :smile:.

We will see if it stays clean and wait a little while while I form a plan (a2g most likely, lc will be shortly down the line for me but still down the line).

edit: I'll probably take a transfer or two as well :smile:. I also forgot to add that I swirled the pins in a pint jar with a cap of bleach (even dropped the smaller one on the FLOOR of the SAB fucketh me and picked it up again dipped it and off we went)


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

Edited by xVadisx (10/25/21 09:05 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27518759 -

Not sure what to make of these. Transferred off a wild cube pin from germ plate. All 3 plates show these linear growths.



Sorry PP5 hard to photo

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27519482 -

From what I can tell from that photo they look good.

A clone from a beautiful phenotype showing some life:


And a solid T1:


Edited by multifractal (10/27/21 02:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27519825 -

I streaked some agar plates with spores from a print 3 days ago in a SAB, then bagged the plates 2 at a time in new ziploc bags inside the SAB.

The plates are not wrapped, and are stored at 26C upside down to stop condensation on the agar.

So far, 5 of the 6 plates are showing the faintest signs of growth in the vicinity of the spores but too early to tell (to my laymans eye) whether it is myc or mould. It reaches out like myc so I don't think its bacteria anyway.

Question to you fine folks:

With unwrapped but bagged plates, how frequently are you comfortable handling them? I'm confident the bags are sealed, but mindful that I will eventually need to remove the plates from the bags to do xfers. So far, I have not handled the bags with gloves or used ISO. Should I?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nigel Stephens]
    #27519840 -

Why dont you wrap them, clingwrap is cheap and works great.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0ptiquest]
    #27519858 -

I have before, - well using gardeners grafting tape rather than clingwrap, but honestly found I was quite clumsy inside the SAB doing that. Knowing that the ziplocs are sterile out the packet is quite reassuring, though I do acknowledge some obvious downsides. I guess this is an experiment, will wrap any xfers if the germ plates make it that far

Is parafilm like Marmite? I actually hate it but know that many love it

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nigel Stephens] * 1
    #27519868 -

Cling wrap is best IMO. I was all like but parafilm stretches, but ya know it brwaks too.

Need to get me a pipe cutter.


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat] * 2
    #27519870 -

I'm a big fan of the cling wrap


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #27520248 -

Team cling wrap for the win. I've been using a bread knife to cut mine. I started out using a box cutter but I ended up with a lot of snaggy edges.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27520253 -

multifractal said:
From what I can tell from that photo they look good.



Thanks guess I'm not used to this manure/grain water. More sectoring then I'm used to. LC time

Going to leave these in a cold basement trying to clone for colder resistant fruits. If that works.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus]
    #27520317 -

Does the knife work better?


You definitely have to be careful with a box cutter. I’m pretty good at it now but I’ve had a few rolls that were super irritating cuz there’s a jagged piece that would snag and randomly cut the cling off mid wrap.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27520367 -

I really like the bread knife. The serrations make it more of a saw. You still have to be careful but I can cut a roll in about 5 seconds.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario]
    #27520428 -

:nohellno:


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27520459 -

Almost every kitchen benefits with a cleaver around. Bulletproof
Very useful when navigating through meats with bones and of course slicing through cling wrap rolls :crazy2:



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.

Edited by Atomsplit (10/27/21 06:55 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27520499 -

I am using a ratchet lopper. Just put a piece of corrugated cardboard under the cling roll so it doesn't get mangled by the notched side.
It helps as well if the blade is clean.



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🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Baba Yaga] * 2
    #27520585 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
    #27520692 -

aNewBeginning said:



Bruh!! Nice!!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27520706 -

I 100% support cling wrap. So cheap and easy.

Never had a problem using even the cheap dollar store version.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Big_Dub]
    #27520715 -

:whathesaid:
cling wrap is sold everywhere and easy to work with.
I've tried parafilm once and found it a pita.
Maybe I had old stock?


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.

Edited by Atomsplit (10/27/21 10:47 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Atomsplit]
    #27520736 -

I prefer cling because it's ubiquitous, but I think that people use parafilm incorrectly, I followed RR' example when I first started and that method is incorrect.

This is how to properly use parafilm:


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