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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker

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OfflineAlegalize
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Registered: 02/23/05
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Potato Dextrose Agar X Malt Extract Agar
    #3832183 - 02/25/05 10:45 AM (11 years, 9 months ago)

Will PDA work as well as MEA for culture os Psilocibin Mycelium in petri dishes?


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InvisibleDamn_Skippy
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Registered: 01/25/05
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832219 - 02/25/05 10:56 AM (11 years, 9 months ago)

why not?


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~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."


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OfflineAlegalize
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Damn_Skippy]
    #3832221 - 02/25/05 10:56 AM (11 years, 9 months ago)

Don?t know, maybe different kinds of nutrients...


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InvisibleDamn_Skippy
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832225 - 02/25/05 10:57 AM (11 years, 9 months ago)

nope... either will work great


--------------------
~Skipp
"I like cigarettes, Miss Taggart. I like to think of fire held in a man's hand. Fire, a dangerous force, tamed at his fingertips. I often wonder about the hours when a man sits alone, watching the smoke of a cigarette, thinking. I wonder what great things have come alive from such hours. When a man thinks, there is a spot of fire alive in his mind---and it is proper that he should have the burning point of a cigarette as his one expression."


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Invisibletripndicular
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Posts: 2,791
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832282 - 02/25/05 11:21 AM (11 years, 9 months ago)

PDA is just fine for growing mush myc. Some strains however tend to get cottony or very arieal when grown on this media. But once you move them to grain they improove.


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So with that said here is our mission statement .

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Invisibleagar
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832305 - 02/25/05 11:38 AM (11 years, 9 months ago)

PDA will. Add a 3 grams of light dry malt & it will go better, IMHO.


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OfflineAlegalize
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: agar]
    #3832355 - 02/25/05 12:01 PM (11 years, 9 months ago)

Well then... Potato is good, but not as good! That?s what I thought...


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OfflineAlegalize
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832369 - 02/25/05 12:08 PM (11 years, 9 months ago)

3 grams dry malt to what quantity of Agar?


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Invisibleagar
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Re: Potato Dextrose Agar X Malt Extract Agar [Re: Alegalize]
    #3832548 - 02/25/05 01:03 PM (11 years, 9 months ago)

To this

? pint Canning Jars & (Fool Proof) Potato Agar
? Slice 1/8 inch thick or grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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