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InvisiblemushboyMDiscord
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hobotechnology]
    #27505919 - 10/15/21 09:58 PM (1 month, 16 days ago)

noice lc
:creepylurker:


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OfflineAtmozFear
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Re: AGAR ENVY! (Anything and All things agar!) [Re: karri0n]
    #27506066 - 10/16/21 01:02 AM (1 month, 16 days ago)

Quote:

karri0n said:
I've been holding onto these for a bit. I wanted to make sure it was viable before posting it.

Blackstrap Molasses.

Blackstrap molasses has about half the sugar of LME and approximately 1000% of the various micronutrients, namely calcium, iron, copper, potassium, magnesium, manganese, selenium and vitamin B6. Many of these minerals are dissolved as salts, making them more absorbable than say, in the form of rock gypsum, dolomite, or even the cellulose filled hulls of grain.


Blackstrap molasses agar
8g blackstrap molasses
10g agar
500ml Brita filter water


The plate pin has mold on the plate - sterile technique problems on my part. However I like the sectoring happening on the one plate.



The gourmets love this stuff. Chicken of the woods and ganoderma Tsugae.


I also did a couple of LC using 1.5g BSM to 500ml filtered water. I would do 1g or .5g next time. It's obviously dark in color, but the clarity is better than any LME broth or grainwater I have ever made. No sediment whatsoever, and I think it's because the BSM starts as a liquid?



PF redspore on the left. It's in a jar with a filter I normally wouldn't use for LC, so I am afraid to swirl it too hard. APE on the right, broken up nicely.

I would love to see someone experiment with this stuff who has a hood and real plates - the condiment cups are ok but you can't get the full picture.



Thanks so much for this!  My LME became moldy and I thought I had nothing but brf left, but you made me realize I could use the BSM that I use in compost tea for my plants!


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AtmozFear]
    #27507137 - 10/16/21 10:03 PM (1 month, 15 days ago)

Both are 13 days old.

Psilo Nat:



Psilo cyan


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InvisibleWall.E
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #27507181 - 10/16/21 10:45 PM (1 month, 15 days ago)

Nats looking good


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Lifeโ€™s shit, but Iโ€™m loving it



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Invisiblemultifractal
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E] * 2
    #27507287 - 10/17/21 12:40 AM (1 month, 15 days ago)



Melmak.


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OfflineA.k.a
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27507561 - 10/17/21 09:35 AM (1 month, 14 days ago)

Yeah that nat culture should be good. Thatโ€™s exactly the type of myc I try to find. Not super rhizo and doesnโ€™t immediately climb the walls.


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InvisibleSamushlove
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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a] * 1
    #27508047 - 10/17/21 04:37 PM (1 month, 14 days ago)

PE/B+ plate has been making pins for months...


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InvisibleBaba Yaga

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Samushlove] * 1
    #27508115 - 10/17/21 05:24 PM (1 month, 14 days ago)

Bacterial Pan cambo clone pinning directly from clone tissue



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Invisiblemultifractal
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Baba Yaga] * 4
    #27508178 - 10/17/21 06:17 PM (1 month, 14 days ago)



More progress on the culture made from spores I took from wild, pasture cubensis. This plates cousins went to grain yesterday.


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OfflineQuirkmeister92
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27508257 - 10/17/21 07:39 PM (1 month, 14 days ago)

:sexymeow:


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Team wheat...
Quirks Tub Prep


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OfflineNichromeS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27508464 - 10/17/21 10:47 PM (1 month, 14 days ago)

Quote:

multifractal said:


More progress on the culture made from spores I took from wild, pasture cubensis. This plates cousins went to grain yesterday.



:popcorn:

I love to see the wild beasties.


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Pheno Hunters


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Invisiblemultifractal
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] * 2
    #27508512 - 10/17/21 11:26 PM (1 month, 14 days ago)

Me while doing SAB work:



You can't show any fear when transferring this culture or it will mount you in a second flat and take its pleasure. Prints available soon.


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InvisibleBaba Yaga

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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27508521 - 10/17/21 11:35 PM (1 month, 14 days ago)

Looks legit :lol:, just make sure your arm holes are big enough.


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
    #27508543 - 10/18/21 12:02 AM (1 month, 14 days ago)

Quote:

multifractal said:
You can't show any fear when transferring this culture or it will mount you in a second flat and take its pleasure. Prints available soon.





Sounds like P-Nat mycelium.


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InvisibleaNewBeginning

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #27508621 - 10/18/21 03:22 AM (1 month, 14 days ago)

Transferred my previously shown Ps. cyan plate to PDA (+yeast) and to LMEA (+yeast).


Ps. Cyan T4 - PDA





Ps. Cyan T4 - LMEA





T1 Ovoid (from print)





TAT Swab T1



The T0 plate got contaminated but the swab was fine so I transferred the swab to a new plate.


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InvisibleDoctor MarioS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning] * 2
    #27509548 - 10/18/21 10:57 PM (1 month, 13 days ago)

This plate is heavily contaminated but I wanted to try to clone this pin anyway.



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Offlineconehead
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario] * 3
    #27509561 - 10/18/21 11:14 PM (1 month, 13 days ago)

First plate I'm proud enough to post here haha. T2 of a clone I took out of my first shoebox. Sent to millet last night!



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Offlinenarmaduke
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Re: AGAR ENVY! (Anything and All things agar!) [Re: conehead]
    #27509631 - 10/19/21 12:25 AM (1 month, 13 days ago)



Are any of these plates salvageable? They come from a super old GT MS syringe


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Invisiblemultifractal
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Re: AGAR ENVY! (Anything and All things agar!) [Re: narmaduke]
    #27509641 - 10/19/21 12:41 AM (1 month, 13 days ago)

You sometimes see growth like this on spore syringe plates. I'd go for the 8 o'clock area of the second plate or some near the bottom of the small circular spot below the center of the third plate and evaluate those transfers to decide if you want to go forward with this culture.

Quote:

conehead said:
First plate I'm proud enough to post here haha. T2 of a clone I took out of my first shoebox. Sent to millet last night!






:thumbup:



This growth pattern was caused by the transfer wedge landing on a small puddle of surface moisture:



Edited by multifractal (10/19/21 01:30 AM)


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InvisibleAtomsplit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] * 2
    #27509664 - 10/19/21 01:28 AM (1 month, 13 days ago)

You all are killing it!

T0C. T-4 was about to grain it but haven't been sleeping well so maybe when I feel rested.



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