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 aNewBeginning
Registered: 06/26/21
Posts: 708
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: Failboat]
#27501035 - 10/11/21 04:17 PM (2 years, 6 months ago) |
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Ps. cyan - Made 3 xfers which will go to grain.
Mazatapec T2 - She's lookin' ropey. I'm not quite sure if I should do another xfer or not.
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
#27501345 - 10/11/21 09:19 PM (2 years, 6 months ago) |
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Your cyan culture looks great. When did you transfer to that particular plate out of curiosity?
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aNewBeginning
Registered: 06/26/21
Posts: 708
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal]
#27501354 - 10/11/21 09:29 PM (2 years, 6 months ago) |
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Quote:
multifractal said:
Your cyan culture looks great. When did you transfer to that particular plate out of curiosity?
Thanks. This is my favourite plate so far. I believe it's T3 from print.
It was transferred 30th of Sep so about 11 days old at time of this photo.
The T2 looked amazing but I wanted to expand the culture.
The new transfers will be going to grain.
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 roarkell
The Roar Master!
Registered: 08/29/19
Posts: 2,223
Loc: Southern WI 
Last seen: 19 days, 11 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
#27501559 - 10/12/21 02:53 AM (2 years, 6 months ago) |
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Quote:
aNewBeginning said:
Ps. cyan - Made 3 xfers which will go to grain.
Mazatapec T2 - She's lookin' ropey. I'm not quite sure if I should do another xfer or not.
In my experience, Maz is very ropey... also very, very side pin-y... heads up!
Id take a few xfers from those thick ropes from 10-noon... see if you get more consistent growth.
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Guerrilla
Bumbaclart
Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
#27504420 - 10/14/21 04:43 PM (2 years, 5 months ago) |
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What do you guys think is better?
A few sterile water baths vs h2o2 for a dirty plate pin?
Been holding onto this one RWxMM plate for months. Parafilm cracked and I tried to clean it up but failed. Don’t know why I kept it but it’s just pinned a nice chonker. Totally white fruit but fat like PE.
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Being pissed on does not make you a real man.
Edited by Guerrilla (10/14/21 04:45 PM)
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Vibetyme
Smoke 1
Registered: 06/08/21
Posts: 1,768
Loc: Florida
Last seen: 22 hours, 29 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#27504456 - 10/14/21 05:11 PM (2 years, 5 months ago) |
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I've got that PE going right now.
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Hindsight
Mad Scientist
Registered: 01/24/21
Posts: 2,706
Last seen: 11 months, 20 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla]
#27504573 - 10/14/21 06:46 PM (2 years, 5 months ago) |
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Quote:
Guerrilla said:
What do you guys think is better?
A few sterile water baths vs h2o2 for a dirty plate pin?
Been holding onto this one RWxMM plate for months. Parafilm cracked and I tried to clean it up but failed. Don’t know why I kept it but it’s just pinned a nice chonker. Totally white fruit but fat like PE.
If the pin is big can't you just split the stem in half and take a tiny chunk out from the middle of the stem?
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 5 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hindsight]
#27504583 - 10/14/21 06:51 PM (2 years, 5 months ago) |
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I've dunked in iso and h2o2 with success. Multiple samples on multiple plates would be smart.
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Hobotechnology
PEU bandit
Registered: 12/19/20
Posts: 502
Loc: In your bushes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
#27504877 - 10/14/21 10:36 PM (2 years, 5 months ago) |
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First stone on agar...two months later of initial inoculation
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multifractal
Student
Registered: 05/08/15
Posts: 1,019
Loc: Cumtown
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hobotechnology] 1
#27504901 - 10/14/21 11:25 PM (2 years, 5 months ago) |
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Check this out. It's a very round (melmak) pin growing upside down.I think it might have originated from a knot on the side of the petri dish then began growing downwards.
Also some nice Mel×PEU germination plates
A few of obvious sectors to go after on the right plate.
Edited by multifractal (10/15/21 12:52 AM)
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Hobotechnology
PEU bandit
Registered: 12/19/20
Posts: 502
Loc: In your bushes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] 1
#27504906 - 10/14/21 11:30 PM (2 years, 5 months ago) |
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Successful transfer of stoneage. Good work doctors.
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The Fresh Prints
Smell ya later
Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: AGAR ENVY! (Anything and All things agar!) [Re: multifractal] 1
#27504921 - 10/14/21 11:50 PM (2 years, 5 months ago) |
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Quote:
multifractal said:
Also some nice Mel×PEU germination plates
A few of obvious sectors to go after on the right plate.
Some tasty bits on the left plate too
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aNewBeginning
Registered: 06/26/21
Posts: 708
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hobotechnology] 1
#27504926 - 10/14/21 11:58 PM (2 years, 5 months ago) |
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Quote:
Hobotechnology said:
Successful transfer of stoneage. Good work doctors.
Nice. Did the stone originate from a jar or a plate?
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Hobotechnology
PEU bandit
Registered: 12/19/20
Posts: 502
Loc: In your bushes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
#27504935 - 10/15/21 12:03 AM (2 years, 5 months ago) |
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Ms plate i knocked up two months ago.
Used it for a few transfers and put it away. Stone was not their 1 week ago so that fucker just exploded.
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aNewBeginning
Registered: 06/26/21
Posts: 708
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hobotechnology] 1
#27504937 - 10/15/21 12:05 AM (2 years, 5 months ago) |
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Quote:
Hobotechnology said:
Ms plate i knocked up two months ago.
Used it for a few transfers and put it away. Stone was not their 1 week ago so that fucker just exploded.
That's crazy. I'm hoping for the same 
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myco.bay
Stranger
Registered: 09/05/21
Posts: 85
Last seen: 5 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: aNewBeginning]
#27505766 - 10/15/21 05:38 PM (2 years, 5 months ago) |
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Quote:
aNewBeginning said:
Ps. cyan - Made 3 xfers which will go to grain.
Mazatapec T2 - She's lookin' ropey. I'm not quite sure if I should do another xfer or not.
That maz plate looks ropey af!
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karri0n
Mind Traveller
Registered: 08/29/20
Posts: 698
Last seen: 26 days, 5 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: myco.bay]
#27505852 - 10/15/21 06:35 PM (2 years, 5 months ago) |
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I've been holding onto these for a bit. I wanted to make sure it was viable before posting it.
Blackstrap Molasses.
Blackstrap molasses has about half the sugar of LME and approximately 1000% of the various micronutrients, namely calcium, iron, copper, potassium, magnesium, manganese, selenium and vitamin B6. Many of these minerals are dissolved as salts, making them more absorbable than say, in the form of rock gypsum, dolomite, or even the cellulose filled hulls of grain.
Blackstrap molasses agar
8g blackstrap molasses
10g agar
500ml Brita filter water
The plate pin has mold on the plate - sterile technique problems on my part. However I like the sectoring happening on the one plate.
The gourmets love this stuff. Chicken of the woods and ganoderma Tsugae.
I also did a couple of LC using 1.5g BSM to 500ml filtered water. I would do 1g or .5g next time. It's obviously dark in color, but the clarity is better than any LME broth or grainwater I have ever made. No sediment whatsoever, and I think it's because the BSM starts as a liquid?
 
PF redspore on the left. It's in a jar with a filter I normally wouldn't use for LC, so I am afraid to swirl it too hard. APE on the right, broken up nicely.
I would love to see someone experiment with this stuff who has a hood and real plates - the condiment cups are ok but you can't get the full picture.
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Panaeolus Bisporus
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Stipe-n Cap 
Registered: 08/04/12
Posts: 7,748
Loc: Canada 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: karri0n]
#27505867 - 10/15/21 06:50 PM (2 years, 5 months ago) |
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My LME broth is crystal clear at .1%, I've found that lower nutrient levels result in better overall performance.
The lower the nutrient content and the lower the dissolved solids in the water the more clarity your broth will have. I use distilled.
Higher nutrient levels trigger mycelium to slow apical hyphal extension to exploit the abundance of resources, lower available resources triggers an exploratory phase with increased apical extension. This is one of the reasons why low nute agar is used to outrun contamination.
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karri0n
Mind Traveller
Registered: 08/29/20
Posts: 698
Last seen: 26 days, 5 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] 1
#27505882 - 10/15/21 07:18 PM (2 years, 5 months ago) |
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That's why I like the BSM, much lower nutrition than LME at about half with all the weird stuff to support healthy growth, no need to add peptone and yeast and this and that.
I wish my .1% LME came out like that. I use maltoferm but I get solids in there.
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Panaeolus Bisporus
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Hobotechnology
PEU bandit
Registered: 12/19/20
Posts: 502
Loc: In your bushes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: karri0n]
#27505891 - 10/15/21 07:32 PM (2 years, 5 months ago) |
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Atl7 lme
About a gram to 500ml of water
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