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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27526187 -

Love those ropes! @Quirk

:amusedapplause:


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Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27526194 -

10 of em are going into bags today. The others with be sectored and expanded to more plates and some broths.:rockon:


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat] * 1
    #27526203 -




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Gymnopilus luteofolius potency results
Gymnopilus luteus potency results
Active Pluteus potency results
Panaeolus cinctulus potency results
Psilocybe caerulipes potency results
Gymnopilus luteus
Gymnopilus luteofolius
Psilocybe ovoideocystidiata
Psilocybe caerulipes
Panaeolus cinctulus
Pluteus americanus

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Unknownfungi]
    #27526220 -



Does this look like trich to anyone else? I tried germinating a PE swab of mine by tearing off a two fibers and burying them in the plate. This growth popped up 3 days after inoculation in between the two spots where I put swab fibers. I think I know the answer :facepalm:

Also, please forgive how nasty the plate lid looks. These plates got horrid condensation and I'm a moron so I decided to turn them upside down and shake the plates side to side to clear the lid of condensation. I know better, but like I said - I'm a moron.


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27526254 -

probably cobweb / Cladobotryum mycophilum, or could be a whole host of white molds.

could be alright though, take a transfer and see if it gets better. Sometimes germinations are really weird looking I guess from all the genetics fighting it out. But that doesn't look crowded it just looks off.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (11/01/21 11:09 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27526266 -

Rw x twc germinating, finally some clean plates to work with.

I'm also wondering if anyone has had issues with bacteria using yeast in agar?
All of my yeast plates had bacteria everywhere but plates without yeast are spotless.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 1
    #27526366 -

bigfootscreepyuncl said:


Does this look like trich to anyone else? I tried germinating a PE swab of mine by tearing off a two fibers and burying them in the plate. This growth popped up 3 days after inoculation in between the two spots where I put swab fibers. I think I know the answer :facepalm:

Also, please forgive how nasty the plate lid looks. These plates got horrid condensation and I'm a moron so I decided to turn them upside down and shake the plates side to side to clear the lid of condensation. I know better, but like I said - I'm a moron.


First off...you're not a moron...stop that!

Second, this appears to be ok...just give it some more time and keep posting updates! :mushroom2::heart:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27526385 -

Quote:
First off...you're not a moron...stop that!

Second, this appears to be ok...just give it some more time and keep posting updates! :mushroom2::heart:


Thanks for that, tormato :smile: I've got a tendency to be pretty hard on myself. But yes, I'll take a couple transfers and let y'all know how it turns out! I just got worried because I'm not really sure how to be sure if it's mold or mycelium. Thanks for the help!


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 1
    #27526401 -

Grow mold for long enough and you'll get a good eye for what's what.:thumbup:

:notsureif:
Everytime...


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Never give up, never surrender.

Edited by Failboat (11/01/21 01:41 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27526417 -





Penis envy MS on agar, quart of rye, and a half dozen PF jars.
Bottle of 200ml grain soak agar fresh out of the Instapot.


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Two eyes to look, One eye to see.
Prying open my third eye

Edited by MysticMycologist (11/01/21 02:01 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MysticMycologist]
    #27526586 -

PDA Sucks man THAT"S IT NEVER AGAIN Maybe my dextrose is bad or what the hell?
On MEA:







On PDYA:





--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (11/01/21 04:31 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27526622 -

I really like Malt Extract Yeas Agar, MEYA right? Anyway, I tried the charcoal, but I like the tan a little better and well my charcoal plates sucked all around :lol:


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27526657 -

It's growing. Honestly I find it's morphology changes depending on the foods. Who gives a shit if it's clean it's clean.

Grain water is all I use now.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27526839 -

Quote:
bigfootscreepyuncl said:

Thanks for that, tormato :smile: I've got a tendency to be pretty hard on myself. But yes, I'll take a couple transfers and let y'all know how it turns out! I just got worried because I'm not really sure how to be sure if it's mold or mycelium. Thanks for the help!


You'll learn through experience how to identify and distinguish between the two. Looking back at your original post I would wait a little longer before making a transfer...3 days seems fast, but not impossible..time is a good tool when identifying molds and myc... typically molds grow pretty fast compared to mush myc. Post another picture in a couple of days and let's see what ya got! :cheers:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27526974 -

Mother in law from hell over — only had time for basic maintenance. These plates grew out! I think one of the pins is pinning?

Pouring plates in the morning. Would you transfer anything specific or just a whole lot of different transfers? Or just go to grain?



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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27527140 -

I don't know the cause but this doesn't look great. It's dying or something as it grows and has the floccular morphology. Maybe viral. I wouldn't play with those cultures any longer and seek new spores from a new source.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (11/02/21 06:24 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27527201 -

Those two originated from pins taken from jars pinning in vitro and were washed in a cap of bleach:pint of water mix.

I’m already planning on taking spore prints and some more fresh tissue samples so will stick to those!

Thanks for the trained eye :wink:.


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27527211 -

Ah I see.

I don't wipe clone material with anything, especially from a jar or plate. The mushroom is literally a dense sponge of mycelium fibers, any liquid contact will soak into it. You can't really wipe anything off it for mechanical removal and any sanitizer will be soaking into it, and that can't be good.

I would just lay a plate pin whole, or rip the mushroom in half and take internal stem/cap tissue for a full size mushroom. I have extremely good success with cloning and never wipe.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27527225 -

Good to know! I was paranoid about the pin somehow being dirty coming from a not-so-perfect BRF jar -- hence the bleach. I think I got that from watching somebody pick a mushroom while I was hiking when they pulled out a little jar with some bleach water and popped a stipe into it.

I've had *awful awful terrible* luck with agar plates thus far (probably 30-40 plates in with no success* but I'm nowhere NEAR giving up hope. A lot of attempts at MSS -> Agar that just proved so ugly and bacterial. I have a really wonderful variety of MDK that has been a profound trip and I don't want to lose the genetics. Although it might be small, the next few fruits that come off the cakes are slated for prints and tissue samples to plates. I'll do several as it's my first time going tissue -> plate.

From what I've read and from watching videos, it seems like good sterile technique in a SAB, ripping the stipe like a piece of string cheese, and a red hot (and then cooled in agar) scalpel to take a little tiny piece seems to be the procedure? I really would love to get a clean culture from these MDKs (and for practice making clean plates from all of the other varieties I have going!).


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #27527353 -

yep you've got the idea right. If you squeeze the base of the stem to separate the fibers a bit and tear towards the cap you can peel the whole stem and cap in half easily. Then you can make 2 cuts of the stringy stem tissue and lift some fibers out easily. Just do your best to keep your fingers away from the area your going to sample. SAB is great for taking tissue samples because there is no airflow blowing the possibly dirty stuff off the mushroom.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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