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agentstone
Stranger
Registered: 09/08/05
Posts: 7
Last seen: 18 years, 2 months
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LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD!
#4861769 - 10/27/05 10:41 PM (18 years, 3 months ago) |
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I am stumped..... look at this picture.  I poured the agar, let it cool , inoculated, sealed with black tape... incubated.... How do I prevent this horrible condensation? Anyone have experience with this?
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: agentstone]
#4861797 - 10/27/05 10:50 PM (18 years, 3 months ago) |
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you won't get so much condensation... if you pour the agar after it cools down a little bit.. rather than pouring it when its super hot.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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agentstone
Stranger
Registered: 09/08/05
Posts: 7
Last seen: 18 years, 2 months
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: Roadkill]
#4861837 - 10/27/05 10:59 PM (18 years, 3 months ago) |
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hrmm ill try that but dont quite understand how that will prevent the prob Thanks for the tip
anyone else have any other ways to make better dishes?
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smarties
Stranger


Registered: 03/07/05
Posts: 307
Last seen: 13 years, 10 months
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: agentstone]
#4861875 - 10/27/05 11:14 PM (18 years, 3 months ago) |
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im assumeing that as it cools some of the moisture evaporates.
-------------------- you need supplies? clicky clicky
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mskip23
Can It All Be So Simple!


Registered: 06/08/05
Posts: 1,522
Loc: Philly
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: smarties]
#4861909 - 10/27/05 11:23 PM (18 years, 3 months ago) |
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yeah I had that problem at first you just have to wait till it coolsssss down it wont hurt anything accept you might not be able to see your myc growing
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif] [/url
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mskip23
Can It All Be So Simple!


Registered: 06/08/05
Posts: 1,522
Loc: Philly
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: mskip23]
#4861917 - 10/27/05 11:25 PM (18 years, 3 months ago) |
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thats alot of condensation!!! you might want to turn them upside down
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif] [/url
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Anno
Experimenter



Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 19 days, 12 hours
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: agentstone]
#4862562 - 10/28/05 05:30 AM (18 years, 3 months ago) |
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2 things:
1: www.fungifun.org/agar 2: Don't incubate the agar plates in an incubator.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: Anno]
#4862574 - 10/28/05 05:48 AM (18 years, 3 months ago) |
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Quote:
Anno said: 2 things:
1: www.fungifun.org/agar 2: Don't incubate the agar plates in an incubator.
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mskip23
Can It All Be So Simple!


Registered: 06/08/05
Posts: 1,522
Loc: Philly
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: agar]
#4862787 - 10/28/05 08:54 AM (18 years, 3 months ago) |
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nice...I got to try that
-------------------- url=https://files.shroomery.org/files/05-26/988356075-Picture-278.gif] [/url
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 25 days
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: mskip23] 1
#4862987 - 10/28/05 10:01 AM (18 years, 3 months ago) |
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I'd also seal with clingwrap, breatable, as opposed to tape. Pour when cooler (condensation has to do with temp differences) and stack plates as you cook them, and try a cup of warm water on top of each stack of petris as they cool too, this keeps the lids warm, so the warm moist air from the unsolid agar doesnt have a chance to form on the cooler lid of the petri dish. Once solid, you can unstack them and you'll have little condensation.
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hills
"Criminal"

Registered: 10/24/04
Posts: 109
Loc: Earth
Last seen: 16 years, 4 months
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: scatmanrav]
#4944944 - 11/17/05 12:42 AM (18 years, 2 months ago) |
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I'm gathering info about agar before I being agar culturing myself, and I see you guys say to not incubate petris in an incubator.
From the answers given, the incubator doesn't appear to be the primary reason for his condensation...
Ya gotta have proper times for colonization...so why not use an incubator? What alternative is better?
-------------------- I am the key to the lock in your house That keeps your toys in the basement And if you get too far inside You'll only see my reflection
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Pieper1973
Stranger


Registered: 02/09/20
Posts: 69
Last seen: 11 months, 7 days
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: agentstone]
#26566819 - 03/30/20 09:05 AM (3 years, 9 months ago) |
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you either have to wait for the agar to cool down or you should apply a stream of air to evaporate the moisture (but you don´t wonna do that, baecause you wonna keep the agar sterile as possible..
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Surf707
Student of Life


Registered: 01/15/20
Posts: 113
Last seen: 10 months, 27 days
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Re: LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! [Re: Anno] 1
#26566962 - 03/30/20 10:37 AM (3 years, 9 months ago) |
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Quote:
Anno said: 2 things:
1: www.fungifun.org/agar 2: Don't incubate the agar plates in an incubator.
Incubators totally raise the condensation content. I was dealing with that for the past two weeks. I found if I don’t parafilm for two days after the pour all my condensation goes away inside the incubator (90 degrees F).
What I do for my agar to cool down is, while it’s still hot, pour my hottest tap water from the sink into the pot (it’s about 125) and periodically check the temp. As the agar cools, the water around it absorbs the heat. Once the water starts dropping below 125 I know it’s just about time to pour.
Edited by Surf707 (03/30/20 10:38 AM)
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