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Offlinefungophiliac
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test tubes agar
    #3480740 - 12/11/04 02:11 PM (17 years, 11 months ago)

does anyone know if you can sterilize agar inside a test tube or will it spill over? and if not how the hell do you pour into them?


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Invisibleagar
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Re: test tubes agar [Re: fungophiliac]
    #3480748 - 12/11/04 02:17 PM (17 years, 11 months ago)



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Offlinefungophiliac
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Re: test tubes agar [Re: agar]
    #3484027 - 12/12/04 06:37 AM (17 years, 11 months ago)

that did not answer a single one of my questions and i could have dione that search myself, matter of fact i did, though didnt bother to read the threads because they didnt seem to pertain. i read them now because i figured that you may have actually known what was in them and they may say something about preparing slants, however after reading them all and not really learning anytihng new, i now see my first assumption was correct, THEY DO NOT PERTAIN TO THE QUESTION. thanks anyways. i still need to know if slants can be prepared in apressure cooker with agar already in them. the reason i asked was because i didnt see an answer on the boards so i am assuming it isnt there, i could be wrong, if someone knows of a link that might tell me i would be happy to read it but please dont send me something that DOESNT PERTAIN and expect me to read it. thanks, no offense


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why are there so many more horses asses than there are horses?

if you can't duck it, fuck it.
-the makers of duck tape



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Invisibleagar
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Re: test tubes agar [Re: fungophiliac]
    #3484476 - 12/12/04 08:13 AM (17 years, 11 months ago)

Use google if you cannot find your answer - here.web page

You can PC a slant tube, with agar in it. Just don't release pressure & allow it to cool over night - in the PC. If you release pressure FAST - While its still VERY HOT, agar can boil over.


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Offlinefungophiliac
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Re: test tubes agar [Re: agar]
    #3484534 - 12/12/04 08:27 AM (17 years, 11 months ago)

thank you so much. i didnt mean to be a dick or anytihng. i had googled and what not i just wasnt having any luck. that is exactly what i was looking for. " You can PC a slant tube, with agar in it. Just don't release pressure & allow it to cool over night - in the PC. If you release pressure FAST - While its still VERY HOT, agar can boil over. "
this is what is going to determine whether i buy a bunch of disposable petri or a set of tubes. thanks for helping:)


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why are there so many more horses asses than there are horses?

if you can't duck it, fuck it.
-the makers of duck tape



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Invisibleagar
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Re: test tubes agar [Re: fungophiliac]
    #3484629 - 12/12/04 08:45 AM (17 years, 11 months ago)

Half pint Canning Jars & (Fool Proof) Potato Agar

? Grate 1 potato into 2 cups distilled water.

? Boil for 30 minutes, strain, add distilled water to make 1 Liter.

? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.

? Simmer while stirring slowly for 10 minutes.

? Pour into 1/2 pints (about ? to 5/8 in. deep)

? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).

? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)

? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.

? When ready to innoc (in glove box or flow/hood), remove lid, innoc & replace lid.

? Remove storage tape & replace with polyfil, or millipore surgical tape, or tape TYVEK over lid hole.

In a few days, you should see growth


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Offlinehyphae
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Re: test tubes agar [Re: agar]
    #3485013 - 12/12/04 10:48 AM (17 years, 11 months ago)

Funny I just got PMed on this one.
Quote:

"I fill anything from baby food jars to vials and slat tubes 2/3-3/4 full PC upright then cool at a slant so the closest the agar reaches to the mouth/opening is 1/2-1in. These are really for storage of a isolate, "plates" are best for observing sectoring but even agar in the bottom of a wide mouth 1/2pt. will work excellent for isolation and storage when using agar. GL hope this helped somewhat." ...........hyphae




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Offlinefungophiliac
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Re: test tubes agar [Re: hyphae]
    #3494533 - 12/14/04 06:34 AM (17 years, 11 months ago)

heh, yeah thanks hyphae that was me. i really was talking about isolating into tubes and then expanding culture amount from there.


--------------------
why are there so many more horses asses than there are horses?

if you can't duck it, fuck it.
-the makers of duck tape



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Offlinegorilliam
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Last seen: 2 years, 4 months
Re: test tubes agar [Re: hyphae]
    #26758632 - 06/20/20 06:41 AM (2 years, 5 months ago)

i am the gorillla[gradient:#D93B3D,#][/gradient]


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