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 fungophiliac
militantpacifist
Registered: 11/08/04
Posts: 190
Loc: yo mama
Last seen: 19 years, 2 months
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 test tubes agar 
#3480740 - 12/11/04 05:11 AM (19 years, 11 months ago) |
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does anyone know if you can sterilize agar inside a test tube or will it spill over? and if not how the hell do you pour into them?
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why are there so many more horses asses than there are horses?
if you can't duck it, fuck it.
-the makers of duck tape
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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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fungophiliac
militantpacifist
Registered: 11/08/04
Posts: 190
Loc: yo mama
Last seen: 19 years, 2 months
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Re: test tubes agar [Re: agar]
#3484027 - 12/11/04 09:37 PM (19 years, 11 months ago) |
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that did not answer a single one of my questions and i could have dione that search myself, matter of fact i did, though didnt bother to read the threads because they didnt seem to pertain. i read them now because i figured that you may have actually known what was in them and they may say something about preparing slants, however after reading them all and not really learning anytihng new, i now see my first assumption was correct, THEY DO NOT PERTAIN TO THE QUESTION. thanks anyways. i still need to know if slants can be prepared in apressure cooker with agar already in them. the reason i asked was because i didnt see an answer on the boards so i am assuming it isnt there, i could be wrong, if someone knows of a link that might tell me i would be happy to read it but please dont send me something that DOESNT PERTAIN and expect me to read it. thanks, no offense
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why are there so many more horses asses than there are horses?
if you can't duck it, fuck it.
-the makers of duck tape
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Use google if you cannot find your answer - here.web page
You can PC a slant tube, with agar in it. Just don't release pressure & allow it to cool over night - in the PC. If you release pressure FAST - While its still VERY HOT, agar can boil over.
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fungophiliac
militantpacifist
Registered: 11/08/04
Posts: 190
Loc: yo mama
Last seen: 19 years, 2 months
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Re: test tubes agar [Re: agar]
#3484534 - 12/11/04 11:27 PM (19 years, 11 months ago) |
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thank you so much. i didnt mean to be a dick or anytihng. i had googled and what not i just wasnt having any luck. that is exactly what i was looking for. " You can PC a slant tube, with agar in it. Just don't release pressure & allow it to cool over night - in the PC. If you release pressure FAST - While its still VERY HOT, agar can boil over. "
this is what is going to determine whether i buy a bunch of disposable petri or a set of tubes. thanks for helping:)
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why are there so many more horses asses than there are horses?
if you can't duck it, fuck it.
-the makers of duck tape
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Half pint Canning Jars & (Fool Proof) Potato Agar
? Grate 1 potato into 2 cups distilled water.
? Boil for 30 minutes, strain, add distilled water to make 1 Liter.
? Add 2 tbsp agar, 1 tbsp dextrose or corn syrup, 1/2 tsp nutritional yeast.
? Simmer while stirring slowly for 10 minutes.
? Pour into 1/2 pints (about ? to 5/8 in. deep)
? Place on lids, not tight, and pressure cook at 15 psi for 30 minutes (cooking too long may/will caramelize the sugars).
? Cool gradually so you don't boil the agar. Tilt pressure cooker as it cools so you will get a slant on the agar. (if you want one)
? Once cooled, tighten lids & tape over lid hole with alc swabbed Scotch brand clear storage tape.
? When ready to innoc (in glove box or flow/hood), remove lid, innoc & replace lid.
? Remove storage tape & replace with polyfil, or millipore surgical tape, or tape TYVEK over lid hole.
In a few days, you should see growth
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hyphae
born to grow
Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 6 months
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Re: test tubes agar [Re: agar]
#3485013 - 12/12/04 01:48 AM (19 years, 11 months ago) |
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Funny I just got PMed on this one. Quote:
"I fill anything from baby food jars to vials and slat tubes 2/3-3/4 full PC upright then cool at a slant so the closest the agar reaches to the mouth/opening is 1/2-1in. These are really for storage of a isolate, "plates" are best for observing sectoring but even agar in the bottom of a wide mouth 1/2pt. will work excellent for isolation and storage when using agar. GL hope this helped somewhat." ...........hyphae
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal 
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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fungophiliac
militantpacifist
Registered: 11/08/04
Posts: 190
Loc: yo mama
Last seen: 19 years, 2 months
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Re: test tubes agar [Re: hyphae]
#3494533 - 12/13/04 09:34 PM (19 years, 11 months ago) |
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heh, yeah thanks hyphae that was me. i really was talking about isolating into tubes and then expanding culture amount from there.
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why are there so many more horses asses than there are horses?
if you can't duck it, fuck it.
-the makers of duck tape
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gorilliam
Stranger
Registered: 06/19/20
Posts: 1
Last seen: 4 years, 4 months
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 Re: test tubes agar [Re: hyphae]
#26758632 - 06/19/20 09:41 PM (4 years, 5 months ago) |
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i am the gorillla[gradient:#D93B3D,#][/gradient]
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