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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg] * 5
    #28154296 -

Pan bisporus T1s, x-post from LAGM. Spores streaked Jan 1st.



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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: SirPsycho] * 1
    #28154338 -

Post deleted by cambocyan

Reason for deletion: .


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Re: Official Copelandia/Panaeolus Thread [Re: cambocyan]
    #28154726 -

Happy Tuesday all you panheads!!! The bispo sounds great. I picked the spore print (because it was good sized) so that's what I got my first agar to work with pans. Just nocced up the jars. Hurry up and wait. I used the agar after 2 transfers and it looked really clean. Fingers crossed! I hope all of your tubs and tents are rockin!!! Peace friends and neighbors!! 😎✌️


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Just chillin like a Villain and talking to like minded individuals!!
             


              Mush Love!!
                😎✌️

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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] * 3
    #28154767 -

Hey

You guys forgets the best reson to grow bispo is its one of the most beautiful pans for the eyes. It's should  also be a little Harder to grow and I think we all here like the challenges in the hobby.

Edited by THE MYCOSMITH (01/24/23 01:11 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 2
    #28155087 -

Repost from dealing with contam thread:

This was the tray about 16hours ago:


This was like 10 minutes ago (before I tossed it)


One of my two Nec-D trys fell to the Trich-Devil >.< My other tray is still looking good, I sure hope that stays that way. Threw the Trich-tray out imidiatly. When I flipped it out of the tray, the bottom was already halfway green >.<

Edit: So I am still a failure until I at least can harvest one fully grown pan ^^

Edit edit: I also checked the bottom of my other tray (which is thankfully also showing tiny pins), it doesn't show anything sketchy (kind of a white-seethrough plastic). So fingers crossed :smile:

Hope at least some people can get their eye schooled on how to see trich maybe a bit better :shrug:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Edited by Shrimps (01/24/23 10:24 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28155239 -

well goddamnit :facepalm3:

but yes fingers crossed for you on the other one mate

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #28155425 -

THE MYCOSMITH said:
Hey

You guys forgets the best reson to grow bispo is its one of the most beautiful pans for the eyes. It's should  also be a little Harder to grow and I think we all here like the challenges in the hobby.



I don't forget :lol: But Bunnell is also incredibly gorgeous. At least the ones I've grown (look at my journal).

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps]
    #28155685 -

Shrimps said:
Repost from dealing with contam thread:

This was the tray about 16hours ago:


This was like 10 minutes ago (before I tossed it)


One of my two Nec-D trys fell to the Trich-Devil >.< My other tray is still looking good, I sure hope that stays that way. Threw the Trich-tray out imidiatly. When I flipped it out of the tray, the bottom was already halfway green >.<

Edit: So I am still a failure until I at least can harvest one fully grown pan ^^

Edit edit: I also checked the bottom of my other tray (which is thankfully also showing tiny pins), it doesn't show anything sketchy (kind of a white-seethrough plastic). So fingers crossed :smile:

Hope at least some people can get their eye schooled on how to see trich maybe a bit better :shrug:



that sucks. i was rooting for you. but good job noticing the trich before it turned green. you noticed it a full 24 hours before it turned green :thumbup:


why do you think your subs are contaminating? how long do they take to colonize? maybe they are too wet? maybe your spawn is dirty?


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(Yes, the egg is real)

How to post pics

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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg]
    #28155718 -

Well, since the sub and casing are both exactly the same and only one tray has shown contamination I have a quite good guess what could have cause id. We have some small plant flies that are flying around (I am already at war with them and I've almost won), but almost none now. The FAE holes are not taped, so maybe such a Fly could have contaminated the one tray.

Other reason could be that (they were from two different spawn jars) the Jar was already contaminated but I've didn't notice it there :shrug:

I can't be 100% sure what caused it, but those two are my guess.

The colonialization on both trays was relatively fast (6-7days) and after I applied the casing (3days later) I saw the first pins and the sketchy trich building up in the one tray.

I am also using straw in my sub, so that could have been a factor, but then the other tray must have also already shown a trich infection, why I don't see that that would have been a problem.


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 3
    #28155723 -

Since spreading some Gnatrol powder in flowerpots, I haven't seen a single one. Big win.

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28155728 -

Jup have been doing that since last week and some yellow glue traps. They are almost eradicated,... just some lonesome survivors which are being hunted down. So a matter of days and they plague is gone :smile:


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28155749 -

Shrimps said:
Other reason could be that (they were from two different spawn jars) the Jar was already contaminated but I've didn't notice it there :shrug:




This is unlikely. Based on the tests I have run, if you have trich in your jars or plates, will absolutely know it and very quickly. It is most likely introduced to your grow during or after spawning. In my own experience, proper pasteurization is a very important part of growing pans and minimizing contamination.

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28155769 -

yeah but both trays have gotten the casing as well as the substrate out of the same bag. So if it would have been itroduced through the sub or casing then both should have gotten trich?

I think it came from the flies. But that's just a guess ^^


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28155793 -

Aside from eliminating the flies or gnats, I would recommend switching your casing material to peat/verm + lime. I don't think it had anything to do with the trich outbreak, I just think it would make for a better casing layer for pans. I'm rooting for your other tray and/or next time!

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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 1
    #28155819 -

Shrimps said:
yeah but both trays have gotten the casing as well as the substrate out of the same bag. So if it would have been itroduced through the sub or casing then both should have gotten trich?

I think it came from the flies. But that's just a guess ^^



Yes it absolutely could have come from the flies. But it could have also been introduced through the sub or casing or tubs themselves. All that said, the main point I was trying to make is that it didn't come from your jars, so if you are looking for ways of reducing risk or increasing cleanliness or changing your process, I would suggest focusing on all the things that take place after your jars are colonized and then opened to spawn to bulk.

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #28156321 -

Hello Pan family. That's my first post here but I went through almost all post on this thread and I am confident enough to start my journey too. This is the best TTBVI(from GordoTEK) T1 plate(got plenty more but I think this one is the strongest and quickest)
What do You think about this one?
Made like 10  T2 transfers from this plate and I plan to inoculate the rye grain with them.


BTW.
What do You suggest to add to rye grain for spawn? I am soaking rye for 16-24h, then simmer for 10-15min until it is a bit drier.
Thanks in advance and mush love guys πŸ„πŸ„

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #28156336 -

Hindsight said:
Shrimps said:
yeah but both trays have gotten the casing as well as the substrate out of the same bag. So if it would have been itroduced through the sub or casing then both should have gotten trich?

I think it came from the flies. But that's just a guess ^^



Yes it absolutely could have come from the flies. But it could have also been introduced through the sub or casing or tubs themselves. All that said, the main point I was trying to make is that it didn't come from your jars, so if you are looking for ways of reducing risk or increasing cleanliness or changing your process, I would suggest focusing on all the things that take place after your jars are colonized and then opened to spawn to bulk.



just my two cents... bacterial spawn can also weaken your sub and increase the chances of mold taking hold, especially if you make other mistakes like overhydrating your substrate.

but like you said, i also like to think of things in terms of risk...

if you have some bacteria in your spawn, it increases your risk.
if you overhydrate your sub while prepping it, it increases your risk.
if you pasteurize your sub incorrectly, it increases your risk.
if you have a fly infestation in your house, it increases your risk.
if you have an unusually high spore load in your house, it increases your risk.
if you choke out your subs and make them try to colonize in an anaerobic environment, it increases your risk
if you put a heating pad under your substrate, it increases your risk.
and so on...
one of these things on its own doesn't always spell disaster. it just means you increased risk for things later down the road, especially if you are making more mistakes later during the process.


--------------------

(Yes, the egg is real)

How to post pics

Edited by FriedEgg (01/25/23 01:13 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: kondu]
    #28156339 -

kondu said:


BTW.
What do You suggest to add to rye grain for spawn? I am soaking rye for 16-24h, then simmer for 10-15min until it is a bit drier.
Thanks in advance and mush love guys πŸ„πŸ„



i'd send that to grain :thumbup:

what do you mean "add to rye grain"? i'm not aware of anyone adding anything to grain. you just hydrate it, sterilize it, and then inoculate it. :shrug:


--------------------

(Yes, the egg is real)

How to post pics

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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
    #28156441 -

ruawakeyet said:
Aside from eliminating the flies or gnats, I would recommend switching your casing material to peat/verm + lime. I don't think it had anything to do with the trich outbreak, I just think it would make for a better casing layer for pans. I'm rooting for your other tray and/or next time!



Ah I adjust the PH of my casing with lime ;-)


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Official Copelandia/Panaeolus Thread [Re: FriedEgg] * 3
    #28156552 -

FriedEgg said:
but like you said, i also like to think of things in terms of risk...

if you have some bacteria in your spawn, it increases your risk.
if you overhydrate your sub while prepping it, it increases your risk.
if you pasteurize your sub incorrectly, it increases your risk.
if you have a fly infestation in your house, it increases your risk.
if you have an unusually high spore load in your house, it increases your risk.
if you choke out your subs and make them try to colonize in an anaerobic environment, it increases your risk
if you put a heating pad under your substrate, it increases your risk.
and so on...
one of these things on its own doesn't always spell disaster. it just means you increased risk for things later down the road, especially if you are making more mistakes later during the process.



Yup, agree with all of this. I have even made some posts recently in my trich experiment thread about the impact of bacterial spawn on trich contamination. It is something I will be running some tests on once I finish my current round of tests.

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds


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