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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 7
    #28137474 - 01/13/23 03:57 AM (1 year, 16 days ago)

This is a ezdialed Monotub which I just filled with water and placed a rack inside for my two trays of Nec-D. This time my pan-grow looks much more promising than my first attempt with pans :laugh: Spawned two days ago from grains to composted cow-poo/straw/verm/Gypsum with 1 tray 1:3 and the other is about 1:2,5.

I am also not misting or anything. Just letting them do their thing ^^ Wish me luck guys!



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Edited by Shrimps (01/13/23 04:03 AM)


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28137483 - 01/13/23 04:26 AM (1 year, 16 days ago)

Quote:

Baba Yaga said:
This is a unmodified Nec-D tub. Lid was flipped two days after spawn and placed on top of the tub with blue-tac to create a 4-5mm gap all around. Photo taken 5 days after spawn. This tub hasn't been misted since it was spawned. Lots of knots have appeared during the last 24hrs. Will start misting in 12 hrs.






Here's an idea: Don't mist at all and see how far they can go while being neglected (aside from harvesting ofc).


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Onlinegoatboy666
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Re: Official Copelandia/Panaeolus Thread [Re: Tweeq] * 1
    #28137566 - 01/13/23 06:42 AM (1 year, 16 days ago)

Thanks for the reply.  I know exactly what you mean. Had a particularly difficult semilanceata a few years back and couldn’t believe what I had just experienced.

The start of the trip was fast and barely had time to hold onto my hat before my bearings on reality were torn away.  A lesson learned in β€˜giving in’ to the trip.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 3
    #28137660 - 01/13/23 08:51 AM (1 year, 16 days ago)

Quote:

Adas said:
Quote:

Baba Yaga said:
This is a unmodified Nec-D tub. Lid was flipped two days after spawn and placed on top of the tub with blue-tac to create a 4-5mm gap all around. Photo taken 5 days after spawn. This tub hasn't been misted since it was spawned. Lots of knots have appeared during the last 24hrs. Will start misting in 12 hrs.






Here's an idea: Don't mist at all and see how far they can go while being neglected (aside from harvesting ofc).





Thought about it but then I remembered what happen to the first Nec-D tub I ran where the surface around the holes stayed dry for too long which resulted in bold spots and smaller fruits close to these areas.



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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 3
    #28137687 - 01/13/23 09:11 AM (1 year, 16 days ago)

The interesting part of it is that all these knots have formed in only 5 days after spawning although not a single water droplet has been on the surface. No moisturizing & evaporation cycle so far but I gave it a very light mist just now.


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OfflineSingularFusion
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28137790 - 01/13/23 10:50 AM (1 year, 16 days ago)

love what you guys are doing over here to improve access to the species Baba

I hope to join you soon, have some Bisporus and Pan Jam in jars already and working up some others for LAGM hopefully, germ has been pretty poor looking though sadly

see you guys when I circle back around:rockon:


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OfflineGreg
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28137794 - 01/13/23 10:52 AM (1 year, 16 days ago)

Never grown pans before, what can I expect in terms of colonization speed on MEA and in general?
I have one germination plate each of cambo and cyan and they're moving slow compared to my cubes and tamps.

Here's the cambo, the cyan is even smaller:


Tamps prepped the same day already on T1 and recovering from transfer:


Edited by Greg (01/13/23 10:53 AM)


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OfflineCosmikktraveler
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Re: Official Copelandia/Panaeolus Thread [Re: Greg] * 2
    #28137807 - 01/13/23 11:06 AM (1 year, 16 days ago)

Quote:

Greg said:
Never grown pans before, what can I expect in terms of colonization speed on MEA and in general?
I have one germination plate each of cambo and cyan and they're moving slow compared to my cubes and tamps.

Here's the cambo, the cyan is even smaller:


Tamps prepped the same day already on T1 and recovering from transfer:





i don't know for MEA but if you want, Asura has make a thread about agar phenotype for pan cyan :

https://www.shroomery.org/forums/showflat.php/Number/26943468


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OfflineGreg
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Re: Official Copelandia/Panaeolus Thread [Re: Cosmikktraveler]
    #28137813 - 01/13/23 11:10 AM (1 year, 16 days ago)

Good info, thank you! :mushroom2:


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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Greg]
    #28137858 - 01/13/23 11:49 AM (1 year, 16 days ago)

I see, Baba. These holes are far too large. Maybe small numerous ones would work best.

Greg, best of luck!


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #28137916 - 01/13/23 12:46 PM (1 year, 16 days ago)

Had good success with this type of tubs before, just burned this one cause I pushed it too far with FAE from the start. These tubs I made 7 years ago when a hole setup like this was standard. At the moment I am very satisfied with how the unmodified tubs are working out, it's the most even FAE I had so far, will rock em like that for a while.


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28137920 - 01/13/23 12:49 PM (1 year, 16 days ago)

My Bispo tub will get colonized soon. I'll let it sit without a casing just to test the theory that casing isn't necessary. And maybe I'll case a part of the surface with the Spruce sawdust to test whether it can even colonize it. :lol:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #28137967 - 01/13/23 01:32 PM (1 year, 16 days ago)

That is going to be interesting.

Next time try giving FAE right from the start or shortly after, this makes uncased work even better.


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #28137977 - 01/13/23 01:50 PM (1 year, 16 days ago)

I have 6 large holes already open, and 10 holes covered with tape with ~15 pin-holes each. It's not plenty of FAE, but it's good.


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 3
    #28137980 - 01/13/23 01:54 PM (1 year, 16 days ago)

Sounds great.


8 Day old Jambo tub unmodified. Flipped lid one day after spawn.



Edited by Baba Yaga (01/13/23 02:04 PM)


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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 2
    #28138001 - 01/13/23 02:08 PM (1 year, 16 days ago)

I've tried 3 grows with the following using an identical process ...
cambodginiensis : Thailand, Suphanburi Goliath
cambodginiensis β€œJambo”
Cyan Central Florida
Cyan Brooksville

The experience has been humbling, As they are way less forgiving than cubes, not sure if my inoculation/spawining technique is not clean enough, leaving bacteria fighting with the myc, or it's the fruiting conditions... though the more I read through the posts on this thread, the more I think success might rely more on genetics.

I've gotten fairly decent results with the Cyan Brooksville - caps got around the size of a penny... nothing ( but eventually trich) with the other 3 strains.

the Brooksville...


this one may be more for the marketplace, but does any one want to take pity on me and set me up with a print. I currently have 3 prints from the above pic to trade for a trusted pan-type print. I'll bet the more advanced cultivators could take these Brooksville's farther than I. Feel free to PM me : )

thanks


Edited by mistermushly (01/13/23 02:19 PM)


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InvisibleShrimps
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28138013 - 01/13/23 02:12 PM (1 year, 16 days ago)

Quote:

Baba Yaga said:
That is going to be interesting.

Next time try giving FAE right from the start or shortly after, this makes uncased work even better.




I have my nec-d trays also in a dialed in Monotub with the holes open from the beginning. Just trying to leave them alone and let them do their thing.

But if I may ask, what exactly do you mean that FAE right from the start would make uncased work better? Got my casing soil ready, but maybe I will do the same as you and just test it with one tray uncased :shrug: (maybe I should wait with making such kind of tests until I at least grown pans succesfully xD, but I am just trying to find what works for me^^)


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Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Shrimps] * 2
    #28138412 - 01/13/23 06:07 PM (1 year, 15 days ago)

Is yours an Ez-Dialed Pasty Tub or an older style mono? I had the best results with older style when taping the bottom holes with 2 layers of MP tape and the top holes open or half open. When leaving bottom holes open from the start the sub near them would dry out as shown at the top of this page. Then reduced the tape on the bottom holes to one layer once knots started to form.


In my experience when giving FAE from the start the substrate will often start fruiting/forming knots before the surface growth thickens up. This keeps the surface more open and it doesn't form a thick mat during the first flush. This is to some degree culture and variety dependent though from what I have seen so far. Some cultures do better when you give FAE right from the beginning because they have a tendency to form thick top growth others you can let go for a couple of days before introducing fresh air which means less maintenance/misting in that time.

The Thai Suphan Buri Cambos always have a tendency for thick top growth for me and it was beneficial to give FAE right from the start. Like you can see in the examples below which are all clone grows.


Here is the difference in surface colonization of Thai Suphan Buri between conventionally colonized substrate, fruiting conditions given from 3rd day after spawn and a tub with fruiting conditions right from the start.




This Jambo clone is not forming that much top growth and the surface stays more open. The following photos are showing 4 different tubs 6 days after spawn. First one fruited at spawn, 2nd fruited one day after spawn, 3rd fruited two days after spawn and 4th fruited 4 days after spawn. It shows nicely how the top grows has been affected by the point in time that FAE was given.




Same thing with this Nec-D clone which does really well in both the unmodified and the old school tub. Photos below are showing both tubs 6 days after spawn. 1st one fruited at spawn in old school tub and 2nd lid flipped two days after spawn in unmodified tub.



Edited by Baba Yaga (01/13/23 07:04 PM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: mistermushly]
    #28138428 - 01/13/23 06:20 PM (1 year, 15 days ago)

Quote:

mistermushly said:
I've tried 3 grows with the following using an identical process ...
cambodginiensis : Thailand, Suphanburi Goliath
cambodginiensis β€œJambo”
Cyan Central Florida
Cyan Brooksville

The experience has been humbling, As they are way less forgiving than cubes, not sure if my inoculation/spawining technique is not clean enough, leaving bacteria fighting with the myc, or it's the fruiting conditions... though the more I read through the posts on this thread, the more I think success might rely more on genetics.

I've gotten fairly decent results with the Cyan Brooksville - caps got around the size of a penny... nothing ( but eventually trich) with the other 3 strains.

the Brooksville...


this one may be more for the marketplace, but does any one want to take pity on me and set me up with a print. I currently have 3 prints from the above pic to trade for a trusted pan-type print. I'll bet the more advanced cultivators could take these Brooksville's farther than I. Feel free to PM me : )

thanks





If your fruits look like this then you will have to go back to spores and try again. This is either contamination related (which I think is more likely but don't have absolute prove) or genetic. Either way means back to spores. Pans can be as forgiving as cubes but one has to hunt for these cultures which makes more sense in my book than trying to tailor conditions to suit a weak or picky culture. I had good results with Goliath and Jambo and I have to admit that cambos seem to be less of a raw egg so there is potential to find something good with what you have. Nec-D is treating me well too.


Hang in there and try some more culture hunting, go back to spores if things don't turn out well and take lots of clones once your fruits look healthy.


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InvisibleFriedEggS
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #28138522 - 01/13/23 07:00 PM (1 year, 15 days ago)

yaga, how is your success with uncased jambo?


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