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Telomere
Unravelling


Registered: 12/26/22
Posts: 172
Last seen: 8 hours, 59 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28136028 - 01/11/23 09:11 PM (1 year, 17 days ago) |
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Quote:
ruawakeyet said:
I should have also included how I get my evaporation in the tent, as it is extremely important. I use 2 separate repeat cycle timers. They can be ordered online. These are much different than typical appliance timers. As FriedEgg stated above, they let you create on & off cycles from seconds to minutes long.
On one cycle timer I run the fogger setup. In my setup, it ran for a few minutes. After it shut off, I gave it around 60 to 90 seconds to allow all of the fog to settle on the trays before the other cycle timer kicked on.
The other cycle timer operated a fan-forced electric heater inside the tent. I had it positioned on the bottom shelf facing the fruiting half of the tent, and it also helped keep the floor dry. (Well, dryer.) I had a temperature controller plugged into that cycle timer, and the heater plugged into the controller. I rewired the heater so the fan would run independently from the heating element, but all of that ran on that cycle timer. When it first kicked on, both the heater fan and heating element would turn on. The temp controller would turn off the heating element once it reached the desired temperature. That usually only took about a minute or two. The heater fan would continue to run until that cycle timer turned it off. You could watch the fog dissipate within the first minute of the heater coming on.
There would be a few minutes where everything was turned off to allow the air to stop moving, then the fogger would turn on again and that completed the cycle. I relied on the rise in temperature to lower the relative humidity, rather than evacuating the humid, foggy air out of the tent. The fogger supplied the fresh air exchange when it operated, and I left 3 of the screened vents in the tent open to allow the air to be pushed out. Those vents were positioned near the bottom of the tent, and it worked great to keep CO2 levels low.
There are some issues with this setup, but it wasn't with the fruits. Mainly, the heater was exposed to the really high humidity environment, and it got some rust on some of the parts. It didn't seem to work very well after I took the tent down and went back to using it in my bedroom. When I took it apart again, I found one of the wire connectors had oxidized badly, made a poor connection and got hot, and started to melt some of the wire insulation. I will need to buy a new electric heater if and when I build a new fruiting room. I might also try some different styles of heaters, or configure a way to separate the heater away from the high humidity.
I know Gordo Tek uses open-air fruiting, so if you have a room that's warm enough, (he suggests using around 70 degrees F), you might just skip the tent all together and see what happens. I would still recommend a repeat cycle timer so that you can have near infinite adjustment on your fogger.
To go to my User Gallery, click my name on the left, scroll down to the bottom, and look for User Gallery.
Oh ok I think maybe I can’t get to your gallery cause I’m new?
Yeah I wish I had the space for the open air fruiting… but it will be fun to rig up a tent to grow a wide variety of mushrooms. I like a challenge. I got a 6 disc fogger which I will build into a tote with a computer fan and I have a shelf and a fan heater already, all I need is the tent, exhaust fan and some lights.
About lights, is there any advantage to LEDs over fluorescent?
And another question - I’ve been seeing guys on here saying they are doing ms grows. How is this achieved with agar? I thought the point of agar was to isolate an individual culture?
Edited by Telomere (01/11/23 09:28 PM)
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Telomere
Unravelling


Registered: 12/26/22
Posts: 172
Last seen: 8 hours, 59 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#28136051 - 01/11/23 09:26 PM (1 year, 17 days ago) |
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Quote:
Scotay said:
Hey there T!! I'm somewhat of a newbie here. Just saw you're new so just wanted to say hello and good luck in joining all the panheads in the fun club!! There's a lot of really cool folks in here willing to share advice and their teks and all their success and failure. I've yet to get a full flush enough for a dose but I'm trying!! So happy Wednesday/Thursday to you and happy new year to you as well!! Peace and chicken grease!! 😎✌️
Hey Scotay thanks for the welcome. I’ve been growing weed and brewing beer for 20 years or more. Growing mushrooms is a different ball game. It’s full of challenges and I am humbled to be learning from some amazing growers on here. As much as I’ve researched over the years I am so happy to be actually experiencing the basics rather than just reading about them. Finally! It’s been a long time coming.
What method are you using to fruit your pans?
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28136056 - 01/11/23 09:30 PM (1 year, 17 days ago) |
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LEDs work fine. The rope lights are waterproof, so that's a big plus. I was using a single LED light bulb hanging from the ceiling of the tent, but I was always watching for condensation that could drip down on it. I never did, but that's always a concern.
At some point, I would like to compare a fluorescent tube to LEDs, but then again there's probably not much point in it. I bet they'll all grow mushies just fine.
I also didn't understand what people meant by MS. I used to think it meant skipping agar and shooting spores directly into the growth medium. I learned it is actually a loosely defined term for multi spore or multi "strain", (which is not technically correct), and is still used even for an agar culture that was started from spore. Once a culture has been fruited, and an ideal specimen has been cloned to start a new culture on agar, it is no longer considered multi spore, and is a clone culture.
Basically, a MS culture is an unproven culture, since you won't know how well it fruits until you've tested it. A clone culture is what you end up with after cloning a fruit, but that is still not a true isolate. Clone cultures can still have multiple genetic pairings, and can be further isolated by transferring sectors if they present themselves, which won't always happen.
At least, that's to the best of my knowledge.
Edit: My bad on the User Gallery instructions, I don't have my pictures in a gallery! I just learned something critical. I will work on that and try to fix.
Edited by ruawakeyet (01/11/23 10:15 PM)
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere] 1
#28136064 - 01/11/23 09:37 PM (1 year, 17 days ago) |
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Im leaving town for four days, have pins showing up every few hours across two jars and a shoebox in my tent. How long does it take them to fully mature? Some of them are already 6cm tall with caps that are opening up. Because these are in a small jar, I cant get a better angle for pictures.
Pins:

Opening up caps:

Coming from APEs the last 6 months, Pan Cyans are so fast! I like how much they can change in a day!
I think I'll just let them grow however they want until I return home Sunday night and harvest then.
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: cooleko]
#28136080 - 01/11/23 10:03 PM (1 year, 17 days ago) |
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The PHVs I grew would be fully mature in 4 days from pins. I was also waiting until spores were dropping, and after recent reading, I will probably start harvesting a little sooner. (Someday I hope!)
I don't think they'll be bad if you wait until you get back, but you will probably have some VERY mature fruits. They should be really beautiful, the caps will hopefully be really wavy and gnarly looking, probably turning upwards a lot. I bet they'll still pack a punch!
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 14 hours
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28136082 - 01/11/23 10:07 PM (1 year, 17 days ago) |
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Quote:
Telomere said:
Quote:
Scotay said:
Hey there T!! I'm somewhat of a newbie here. Just saw you're new so just wanted to say hello and good luck in joining all the panheads in the fun club!! There's a lot of really cool folks in here willing to share advice and their teks and all their success and failure. I've yet to get a full flush enough for a dose but I'm trying!! So happy Wednesday/Thursday to you and happy new year to you as well!! Peace and chicken grease!! 😎✌️
Hey Scotay thanks for the welcome. I’ve been growing weed and brewing beer for 20 years or more. Growing mushrooms is a different ball game. It’s full of challenges and I am humbled to be learning from some amazing growers on here. As much as I’ve researched over the years I am so happy to be actually experiencing the basics rather than just reading about them. Finally! It’s been a long time coming.
What method are you using to fruit your pans?
Very cool T!! I'm an old school cannabis guy myself. For the last several years I've been preserving straight land races!! I've grown cubes and that's cool. It's fun. I'm just trying to wrap my head around the pans at this point. It's great to see a thread this old still banging hard and newbies like us trying to figure it out and all the experienced panheads mixing it up and sharing all this knowledge!! I'm happy to "meetcha" my friend and I hope you catch the pan fever!!! Happy Thursday now!!! 😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 14 hours
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28136088 - 01/11/23 10:36 PM (1 year, 17 days ago) |
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Quote:
ruawakeyet said: When you ask about sending some clean-looking plates to grain, are you talking about germination plates or T1; T2; etc. plates? I don't know if I would risk a clean looking germ plate, I've wasted a lot of grain lately, and have grown quite averse to wasting more of it. If you have a clean looking T1 or above, send it!
These are my first good pan plates. .... These are the T1 plates. I have 6 out of 10 that look good. I'd like to do a T2 for the two best looking ones but the other 4 I'm wondering if I could maybe just cut a nice piece from the clean T1 and just drop it into the my grain jar (already PC'd and ready) I'm just stoked to finally have the agar working. I 50/50 grain water agar. 1g of agar powder per 100ml water. Hopefully I don't screw this up!!! I appreciate your time man!!😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#28136103 - 01/11/23 11:22 PM (1 year, 17 days ago) |
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Yeah, sounds like a good plan! I wish you luck!
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28136120 - 01/11/23 11:40 PM (1 year, 17 days ago) |
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Quote:
Telomere said: Oh ok I think maybe I can’t get to your gallery cause I’m new?
I also edited my reply above, but in case you missed the edit, that was my bad. I didn't realize I had to click share for them to show up in my gallery.
I fixed that, as well as sorted them into folders, and added comments on most of them to help explain what the pictures are of.
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mushroom_gnome
Stranger


Registered: 06/30/22
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28136234 - 01/12/23 05:50 AM (1 year, 17 days ago) |
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Are people experiencing necD to be equally potent as pan cyan?
-------------------- Mystery Mushroom
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: mushroom_gnome] 1
#28136249 - 01/12/23 06:20 AM (1 year, 17 days ago) |
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Quote:
mushroom_gnome said: Are people experiencing necD to be equally potent as pan cyan?
As for me Brooksville even stronger - it is extremely visual. The most easy and soft is Peace River.
Edited by ekabpils (01/12/23 06:22 AM)
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Telomere
Unravelling


Registered: 12/26/22
Posts: 172
Last seen: 8 hours, 59 minutes
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 1
#28136281 - 01/12/23 07:03 AM (1 year, 17 days ago) |
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Quote:
ruawakeyet said:
I also edited my reply above, but in case you missed the edit, that was my bad. I didn't realize I had to click share for them to show up in my gallery.
I fixed that, as well as sorted them into folders, and added comments on most of them to help explain what the pictures are of.
Cool now I can see it. That’s really awesome. I wish I had the space to do something like that.
You’ve had some really good results with this PHV!
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Telomere]
#28136426 - 01/12/23 09:40 AM (1 year, 17 days ago) |
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Quote:
Telomere said: Cool now I can see it. That’s really awesome. I wish I had the space to do something like that.
You’ve had some really good results with this PHV!
Thanks! I would say I had some decent results with the PHV. I hope I did it some justice to make JakeOncid proud. I still have yet to pull down a full canopy of good sized fruits.
I hope I'm close to getting past my issues using my SAB. I've moved since the last time I grew those, and have had a difficult time getting started back up again. My basement is just a little too short to use the same grow tent, and I don't want to set it up upstairs for the lack of space. My plan is to build an 8' x 8' or 8' x 6' grow room so I have more space inside to do whatever I want with it.
I will be using basically the same setup with a few small modifications to try.
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cooleko
Augmentum provocatus

Registered: 08/15/22
Posts: 1,010
Loc: Seattle
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 9
#28136682 - 01/12/23 01:42 PM (1 year, 17 days ago) |
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Pan Cyans (BVI) up close and personal. Cross post from Cult. PotD.


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99.99
Stranger


Registered: 12/22/14
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Re: Official Copelandia/Panaeolus Thread [Re: cooleko] 4
#28136696 - 01/12/23 01:54 PM (1 year, 17 days ago) |
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Thai Suphan Buri Cambo grow so insanely fast. It just blows my mind. 4 days ago I put my colonized grain to bulk of 50/50 coir steer manure, and today I’ve already have pins
Edited by 99.99 (01/12/23 03:54 PM)
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: 99.99] 1
#28136849 - 01/12/23 03:33 PM (1 year, 16 days ago) |
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I think it's official that my Bunnell culture is toast. Refuses to colonize the sub that Bisporus devours fine. Oh well, may get my hands on some BVI then 
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Scotay
The Mad Mush Guy

Registered: 05/14/22
Posts: 228
Loc: United States
Last seen: 4 days, 14 hours
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet]
#28137329 - 01/12/23 10:25 PM (1 year, 16 days ago) |
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Quote:
ruawakeyet said:
Quote:
Telomere said: Cool now I can see it. That’s really awesome. I wish I had the space to do something like that.
You’ve had some really good results with this PHV!
Thanks! I would say I had some decent results with the PHV. I hope I did it some justice to make JakeOncid proud. I still have yet to pull down a full canopy of good sized fruits.
I hope I'm close to getting past my issues using my SAB. I've moved since the last time I grew those, and have had a difficult time getting started back up again. My basement is just a little too short to use the same grow tent, and I don't want to set it up upstairs for the lack of space. My plan is to build an 8' x 8' or 8' x 6' grow room so I have more space inside to do whatever I want with it.
I will be using basically the same setup with a few small modifications to try.
Speaking of SAB I used mine tonight. I picked the best 2 plates (original from print) and both were very clean. No green monster showing up as of yet. I streaked 4 plates from 1 and 4 plates from the other for a total of 8 new plates. If these plates are as good as the originals is it wise to drop the agar right on the grains? I hope everybody is having a good week and happy Friday to y'all friends and neighbors!!😎✌️
-------------------- Just chillin like a Villain and talking to like minded individuals!! Mush Love!! 😎✌️
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay] 1
#28137339 - 01/12/23 10:38 PM (1 year, 16 days ago) |
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That should do. I tend to go 2 transfers from germ plate but that could be overkill, especially if all is looking great. FYI, streaking is what you do with spores or LC. Literally making a streak of spores or solution on the plate to spread out the material so you can see individual cultures to choose from as opposed to having them all bunched up. I’m guessing you made transfers?
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ruawakeyet


Registered: 05/03/21
Posts: 1,862
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Re: Official Copelandia/Panaeolus Thread [Re: Scotay]
#28137342 - 01/12/23 10:42 PM (1 year, 16 days ago) |
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Quote:
Scotay said: Speaking of SAB I used mine tonight. I picked the best 2 plates (original from print) and both were very clean. No green monster showing up as of yet. I streaked 4 plates from 1 and 4 plates from the other for a total of 8 new plates. If these plates are as good as the originals is it wise to drop the agar right on the grains? I hope everybody is having a good week and happy Friday to y'all friends and neighbors!!😎✌️

When you say you streaked 4 new plates from a germ plate, do you mean you took transfers, or literally swabbed the germ plate, then streaked that to new plates? Streaking is typically used only for spores to the best of my knowledge. Once one has clean mycelium from germination, small sections are cut out from the leading edge, then transferred to a new plate and allowed to grow out again. That first transfer is what is known as a T1, and if another transfer is made to a new plate, that would be a T2. I'm just confused by the way you are describing what you have done, and don't want to be making incorrect assumptions.
Once you have a clean T1 or T2, etc., they should be sent to grain. The only reason to keep taking more transfers is if you are still seeing contaminants, or if the growth is looking suspect, like visible sectoring; or zones of inhibition; or excessively cottony growth.
In terms of inoculating grain with agar, one can use as small or as large a segment as one desires, everyone has their own preference, and they all can work. It might just take some trial and error to find out what you prefer.
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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] 3
#28137461 - 01/13/23 03:04 AM (1 year, 16 days ago) |
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This is a unmodified Nec-D tub. Lid was flipped two days after spawn and placed on top of the tub with blue-tac to create a 4-5mm gap all around. Photo taken 5 days after spawn. This tub hasn't been misted since it was spawned. Lots of knots have appeared during the last 24hrs. Will start misting in 12 hrs.
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