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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27708124 -

Adas said:
The happy season has officially started! Prepared some flowerpots and added horseshit with Bisporus spawn as a nice bonus! I will make more though. Wish me luck! :crazy2:


Please keep up posted. The season is here too and I'm a couple weeks away from dumping my first bispo grains outside. Actually I'm going to be late. I could have probably started a month ago. I do have a jar of bispo from an MS culture that should be ready within a day or two that I was planning on doing as part of my LAGM grow... but you know what, I'm going to put it outside!


Care to share your flower pot prep, and also why you do in pots instead of the ground? My outdoor pan formula last year was simply to scrape the ground to bare earth, dump colonized grain spawn on the dirt, cover with 1/4" of dried manure, then cover with 1/2" or so of straw and water with a watering can. That's it. It worked well but it's the only way I have done it so I'm sure there are ways of getting even better success. I may try multiple ways with bispos outdoors - pots, ground, ground in frame, with vs without companion grasses, etc.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27708130 -

Thanks for that insight on the punger punch
Adas good luck Hope you get some good spring fruites so we Will see others copy you in the fall.

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27708137 -

THE MYCOSMITH said:
Thanks for that insight on the punger punch
Adas good luck Hope you get some good spring fruites so we Will see others copy you in the fall.


Sure thing. I had a photo in the gallery of the plunger punch but can't find it for some reason so took a new one - this one is side by side.

Top one is just the tubing I posted the link to, that has been cut down to a more handy length (from 12" as purchased). It's very thin so you need to be careful how you cut it.

Bottom one is the same tubing, but with a solid rod that fits perfectly inside, plus the handle, cap and spring. I'm compressing it there enough for the rod to poke out the bottom (as if I was ejecting a stuck plug). If I let go, the spring would retract the rod about 1/2" up into the tube. I never both with this tool anymore and just use the bare pipe at the top. It cools from red hot in 3 seconds and never gets hot to handle.



--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 8
    #27708247 -

working since few months with a little tool I made to transfer really small agar samples (I think it can be useful to have micro samples to select a sector or a clean zone or even using smaller plates...) for agar to agar or agar to small LC it’s relatively easy to make and to use:








I will try to do a write up on this soon..


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27708248 -

:popcorn:


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I :heart: 5318008


NOT a virgin!

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27708283 -

In the ground the slugs are a bigger problem. I will place the pots (hopefully) out of the reach of slugs, a rocky place where I can't dig (there are only few places I can dig because it's not my property). Also, it kinda looks cooler that way to some extent.

Literally all I did was load some horse pellets into a big jar, pour in water, put that into my PC to pasteurize it, then I squeezed the water out (to the extent my bodyweight permits) and filled the pots about 3/4 full. Then I added the un-potted Thyme cluster in the center, sprikled spawn around, added chunks of the shit and covered with some more potting soil. That's all. I should have added sawdust to the shit and perhaps leeched it because it turned out incredibly sticky, and not quite the volume I hoped for.

There will be more but so far so good.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: bigfootscreepyuncl]
    #27708285 -

V.L. That is briliant. Can you please tell why you choose to Bend the tip on the needles.?

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 1
    #27708300 -

It’s bended because if it’s straight the samples will stick to the plate most of the time, if it’s bended you just need to turn the syringe (1/4 to 1/2 turn) and like this the sample stay in the needle that’s the trick! and after you just need to push to the new plate or LC..

Good luck Adas and Insight for your outdoor projects!


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27708306 -

Amazing little contraption, VL! Looks very cool to me, I enjoy these kind of builds.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27708308 -

Mycology MacGyver mode!


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: bigfootscreepyuncl]
    #27708323 -

That's a really cool tool VL... I have wanted something like that for a while. I only use my big punch when making big transfers on clean plates. If trying to get a clean transfer from a really crowded plate, small area, or very dirty plate, I just use the scalpel but a tool like yours would make things easier and more precise.

Is the hollow metal tube just a flat-tipped syringe needle?
I take it the plunger rod that goes into the hollow tube is actually a smaller gauge syringe needle that you pinched and blunted the tip on?


Adas, that all makes sense. When I did my outdoor beds last year I didn't even pasteurize the manure. I might try both options side by side this year and see if there is any difference. I also have a mound of manure I got from a nearby barn that has been outside leaching for a few months... might use some of that too.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27708332 -

That sounds about ideal. Really any manure is gonna be way better than what I have. What I have is probably grain-fed, and definitely not aged. I didnt even leech it this time. I am confident in the Bisporus though, they should still be able to eat it. I only use this shit because it's the best I can comfortably source. And it works.

Edit: Mine is super muddy so it's def not grass-fed


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

Edited by Adas (03/25/22 06:07 PM)

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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27708333 -

V.L said:
working since few months with a little tool I made to transfer really small agar samples (I think it can be useful to have micro samples to select a sector or a clean zone or even using smaller plates...) for agar to agar or agar to small LC it’s relatively easy to make and to use:








I will try to do a write up on this soon..


Very nice VL, I have a clone that has some nice myc but a lot of bac, and taking a more biopsy style wedge would be just the thing I think! I look forward to your write up.


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 2
    #27708361 -

Hindsight said:
That's a really cool tool VL... I have wanted something like that for a while. I only use my big punch when making big transfers on clean plates. If trying to get a clean transfer from a really crowded plate, small area, or very dirty plate, I just use the scalpel but a tool like yours would make things easier and more precise.

Is the hollow metal tube just a flat-tipped syringe needle?
I take it the plunger rod that goes into the hollow tube is actually a smaller gauge syringe needle that you pinched and blunted the tip on?



Yes the hollow metal tube is a cut off needle (the pink ones 18gr I think..) and yes inside to push is a thiner syringe needle (the green) and to make it longer I stick a fine sewing needle at the top of it..

Thanks Coversall! will try to do a write-up soon


--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

If you need some prints (Pans, cubes, tamp, WL…) feel free to PM

Edited by V.L (03/25/22 06:45 PM)

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: V.L] * 1
    #27708383 -

I dig it. What kind of spring is that? Maybe one could modify a metal spring-loaded pen 🤔


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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
    #27708600 -

Those are just beautiful whatever they are!
Well done!
:seriousthumbsup:

ooops I thought i was looking at the end of the thread. This comment pertains to Mr Giggles post re Cints /Bisporus


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What are they saying? Listen carefully, it might be something you need to hear...




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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 2
    #27708607 -

Also 
Wow VL - I am loving your little engineering myco tool design project there!
:raisemyglass:


--------------------
What are they saying? Listen carefully, it might be something you need to hear...




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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn] * 1
    #27708924 -

Why thank you good sir. Idk if you're referring to the fruits or the tiny sclerotia. I was going through the blue pearl thread and the cinct/bispos I have look identical to the other ones that were fruited. I know at least one person ate them and said they were equally, if not more potent than cubensis. Are those tampanensis in your avatar?


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] * 3
    #27709232 -

Hindsight said:
If you go with a perfectly round plug, you should get perfectly round growth. I want that because it helps me identify potential contamination, and also helps me see sectors better.


Is this more of a pan thing? I'm new to the pan world so I can't speak on it with 100% certainty, with cubes that's wrong. I can cut a square, rectangle, use a plate pin and get circular growth on agar. No matter the shape if shit goes wonky and spikey you have issues.


--------------------

Crack's Tek's and Shit


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Re: Official Copelandia/Panaeolus Thread [Re: Crackatoa] * 1
    #27709458 -

No it isn't something I have noticed with pans specifically - I have the same thing happen with cubes. I mean, it makes sense right? If mycelium will all grow outward at the same rate (and why wouldn't it), after a few days, if you measured outward 1cm from each area of your plug, you are not going to end up with a perfect circle if your plug is a triangle or a square. Things don't look really wonky or anything though, but I just find it much easier to see things in a perfect circle.

To use an extreme example, if you pulled a long 2cm fiber from the stipe of a fruit for cloning purposes and laid it down in a plate, you're going to get an oval looking shape, not a perfectly round circle.

I tried looking through old photos to see if I could find some good examples of how a triangle shaped plug ends up growing out as more of a rounded triangle than a perfect circle. I couldn't find a lot of good pics but here is what I could find (these aren't the best looking plates):



Here is another:


Take a look at the one on the left here (ignore the plate on the right which was an accident with too much peptone):


vs circle plugs:





I'm open to being corrected on this, but it is just what I have observed in my experience.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


Extras: Filter Print Post Top
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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