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Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,958
Loc: Hyperspace Chicken Coop
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 2
#27718998 - 04/03/22 05:29 AM (1 year, 9 months ago) |
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A slow culture does not necessarily mean that it's bunk. Some healthy cultures can be slow on grain but go fast once they hit the bulk. I had slow jars and even some that recovered poorly but performed well.
I wouldn't toss it, but those are just my anecdotal 2 cents.
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
#27719002 - 04/03/22 05:34 AM (1 year, 9 months ago) |
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Thanks mate, yeah it looks good, just slow. I use LC 95% of the time as well which might have made me slightly impatient with agar haha. Ok I’ll let it ride.
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Sagescruffy
CH



Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley] 1
#27719797 - 04/03/22 05:33 PM (1 year, 9 months ago) |
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Maybe give it another shake? Haha that’s what I did with mine and now I’ve got my substrate almost fully colonized all ready after 3 days
-------------------- Love.  
Edited by Sagescruffy (04/03/22 05:34 PM)
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight] 1
#27720049 - 04/03/22 08:37 PM (1 year, 9 months ago) |
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Quote:
Hindsight said: I'll second that coir/manure works fine. The only catch is that you should not compress the coir. I know a lot of cube growers will pack the coir down quite a bit. That works well for cubes and it suffocates pans. I did a test with several tubs, and the same clone culture from the same plate, divided up into 4 grain jars. Two were coir/manure, one packed and one not packed. Two were the classic straw/verm/manure unpacked. The packed coir/manure tub never even fully consolidated. It just stalled out very early and no mycelium ever made it to the surface.
As long as I don't compress the coir in the tub, I have good luck with it. I find that I have more consistent results with coir, but I get the chance for better results with straw. I believe this is because the pan mycelium prefers straw, but straw is more of a contamination vector so you are playing the odds on straw tubs more than you are on coir tubs. These days I am not doing much with coir and am instead experimenting with straw alternatives like shredded hemp. But again, coir does work and I have plenty of great trays to show for it, as does Baba.
I haven't played around with fluffed coir yet, just heard about overlay potential and I wanted to simulate its native env
As Gordo says, these species are grass lovers. Straw and manure (has the Nitrogen grass is massively deficient in) and enough low density area to grow its puffy self. My sub is super light and fully colonize in 4 days. Casing Tues. Hopefully the trays Pan out :p
The other perk is a probiotic sub. Should be fairly resistant to contam and push the full BE of my Oats/RGS blend
Edited by Snazz (04/03/22 08:46 PM)
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ekabpils
Exploring Exotics



Registered: 10/20/21
Posts: 167
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz] 1
#27720659 - 04/04/22 10:24 AM (1 year, 9 months ago) |
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NecD is coming:

NecD from the beginning looks a little bit different from other strains.
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Sagescruffy
CH



Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: ekabpils] 1
#27721520 - 04/04/22 10:17 PM (1 year, 9 months ago) |
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Ahh yeaahhh! They do look to be coming up somewhat different. Almost like the mushrooms are there all ready and they’re just coming up
-------------------- Love.  
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PsiloPsychIn
PsiloPsychIn



Registered: 06/17/14
Posts: 8,182
Loc: up north
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Re: Official Copelandia/Panaeolus Thread [Re: Sagescruffy] 1
#27721684 - 04/05/22 02:24 AM (1 year, 9 months ago) |
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Go Team Bunnell! I think there have been six plate pins on this one! I better get around to cloning one while it’s still fresh.

Also I tried about .47 gm of these this weekend and was pretty amazed at how far it took me. Some CEVs and definitely some visual enhancements and Spongle had my mind tying itself in musical fractle pretzels 😊
-------------------- What are they saying? Listen carefully, it might be something you need to hear...
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Adas
Lonely Dreamer



Registered: 12/22/16
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Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: PsiloPsychIn]
#27721706 - 04/05/22 03:34 AM (1 year, 9 months ago) |
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Glad you enjoyed them, Psilo! They are powerful AF! I haven't gotten plate pins yet with them but it might be my agar recipe.
Also, my current grow pushed out like 2 fruits (both grew from the plastic) and nothing else. I'm clueless, I think I'm doing everything the same as before. I might try casing earlier next time or something.
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razero



Registered: 12/23/08
Posts: 96
Loc: Jamaica
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27721820 - 04/05/22 07:50 AM (1 year, 9 months ago) |
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3x different plates of pan trop tried and all have literally almost 0 potency. One left to test but looks like I need to start over from spores again. Wtf really, disappointed.
-------------------- He who does not research has nothing to teach.
Edited by razero (04/05/22 07:51 AM)
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: razero] 1
#27721845 - 04/05/22 08:17 AM (1 year, 9 months ago) |
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Quote:
razero said: 3x different plates of pan trop tried and all have literally almost 0 potency. One left to test but looks like I need to start over from spores again. Wtf really, disappointed.
What do you mean by 3 plates tried? You mean you ate plate pins or something? I’m confused….
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razero



Registered: 12/23/08
Posts: 96
Loc: Jamaica
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Re: Official Copelandia/Panaeolus Thread *DELETED* [Re: SwabMarley]
#27721853 - 04/05/22 08:29 AM (1 year, 9 months ago) |
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Post deleted by razero
Reason for deletion: a
Edited by razero (05/30/23 03:29 PM)
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SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
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Re: Official Copelandia/Panaeolus Thread [Re: razero]
#27721868 - 04/05/22 08:45 AM (1 year, 9 months ago) |
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Quote:
razero said: 3 different transfers taken from multi spore on agar plates. A2G and fruited = literally 0 potency batches. You can filter posts to see pics from one of the 0 potency batches.
Oh right, that’s some shitty luck! I kinda try to go to grain ASAP from spores. Like if it’s clean, send it. Just means you have much more genetic diversity as apposed to isolating further. Maybe give that a go or make 3 or 4 LC’s from different sectors.
Im gunna put some LC to BRF pucks (Josex style) and see if I get any pins popping up. Anyone have any experience with this? Maybe a good way to nail down a fruiting clone early on.
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Snazz
Polymath



Registered: 11/24/15
Posts: 1,584
Loc: Canada
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Re: Official Copelandia/Panaeolus Thread [Re: SwabMarley]
#27721897 - 04/05/22 09:16 AM (1 year, 9 months ago) |
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My recent technique is to germinate on activated charcoal agar (LMEY)
Then transfer once, and if it's a nice poofy culture, I tiger drop into RGS jar (200ml or so)
Let it colonize, then G2G out to 7ish pints of Hull-on Oats
This spreads the genetics quite a bit, as grass seed has huge coverage
Then I monitor the pints, track them for a week or two, and select the best to expand again. The others S2B
Gives offset fruiting times and fair variance imho
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karri0n
Mind Traveller



Registered: 08/29/20
Posts: 698
Last seen: 16 days, 1 hour
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#27722022 - 04/05/22 11:31 AM (1 year, 9 months ago) |
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Pan Bisporus, it looks like after several different tries I've possibly got some pins.
But the stipe looks quite white and the caps quite brown almost like a cube.
Is this what p. bisp should look like as pins?
--------------------
Panaeolus Bisporus
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,270
Loc: Central EU
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Re: Official Copelandia/Panaeolus Thread [Re: karri0n]
#27722091 - 04/05/22 01:15 PM (1 year, 9 months ago) |
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It does look like a Pan, congrats!
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Grim11311
Supporter


Registered: 12/20/19
Posts: 281
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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
#27722138 - 04/05/22 02:11 PM (1 year, 9 months ago) |
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Hi guys. Need a bit of help please. I have a few agar plates with what i hope is pan cyan jamaican strain and was hoping some of these resemble it or are they all fucked?
Some grow thick and some almost like spider webs. Any help would be appreciated
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Sagescruffy
CH



Registered: 10/30/09
Posts: 2,011
Loc: PNW
Last seen: 6 months, 12 days
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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311]
#27722143 - 04/05/22 02:14 PM (1 year, 9 months ago) |
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Grim: sorry I can’t help yah, I’m a pan newb haha
My pans have slowed down a lot. I think my lids weren’t giving them adequate GE since I had them screwed on half way. I unscrewed them and tilted them a little bit I hope this helps, they’re all close! This is the start of day 5
-------------------- Love.  
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Hindsight
Mad Scientist


Registered: 01/24/21
Posts: 2,706
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Re: Official Copelandia/Panaeolus Thread [Re: Grim11311] 1
#27722156 - 04/05/22 02:36 PM (1 year, 9 months ago) |
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Quote:
karri0n said: Pan Bisporus, it looks like after several different tries I've possibly got some pins.
But the stipe looks quite white and the caps quite brown almost like a cube.
Is this what p. bisp should look like as pins?

Yes these are what my bispos look like as well. Caps are deep brown - not like a cyan, and stems are much lighter in color - white to light grey. Cap size is larger than pan cyans too.
Quote:
Grim11311 said: Hi guys. Need a bit of help please. I have a few agar plates with what i hope is pan cyan jamaican strain and was hoping some of these resemble it or are they all fucked?
Some grow thick and some almost like spider webs. Any help would be appreciated

To my eyes, those all look like mold. Second one is tough to tell though because it is too blurry. You don't want a sort of diamond or weblike pattern which is what I'm seeing in those pics. Some overall whispiness is normall but you should see a definitive pattern of lines that radiate outward from the center in an organized way. You will have some "background noise" beyond those outward growing lines but the lines themselves should be distinctive and dominant. What agar formula are you using? I swear by GWA for pans. The growth pattern makes things very easy to sort out.
Here is an example of what I don't want (notice the lack of straight lines):

Here is an example of what I want (notice the pattern of straight lines):
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Grim11311
Supporter


Registered: 12/20/19
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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
#27722175 - 04/05/22 02:57 PM (1 year, 9 months ago) |
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To my eyes, those all look like mold. Second one is tough to tell though because it is too blurry. You don't want a sort of diamond or weblike pattern which is what I'm seeing in those pics. Some overall whispiness is normall but you should see a definitive pattern of lines that radiate outward from the center in an organized way. You will have some "background noise" beyond those outward growing lines but the lines themselves should be distinctive and dominant. What agar formula are you using? I swear by GWA for pans. The growth pattern makes things very easy to sort out.
Used WBS grain water and 2g honey
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Hongosamongus
Stranger
Registered: 03/04/22
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Loc: Dry
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
#27722234 - 04/05/22 03:54 PM (1 year, 9 months ago) |
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What are you using in your "probiotic sub"?
-------------------- I'm a stranger here myself.
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