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OfflineV.L
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V.L micro-agar transfer tool * 17
    #27710061 - 03/27/22 12:58 AM (1 year, 9 months ago)

Hi everyone! Here is a little write up to describe a little tool I made to transfer really small agar samples (can be useful to have micro samples to select a sector, a clean zone or even using smaller plates...) for agar to agar or agar to small LC it’s relatively easy to make and to use:
Can be sterilized in PC & needles can be flame sterilized during a transfer session (just let it cool before taking next sample)
Can be used in very small or deep plates even probably in a slant..
As the sample and the contact size of the tool is small I think it reduce risk of contam (so far I got good results with it even without a flowhood)
As the sample is very small it can help to do precise sector isolations (so potentially reduce number of transfers needed).
Also can be useful for taking a very localized clean spot in a dirty plate.
It’s not difficult technically to practice & it’s relatively quick to use with not a lot of movements...



Materials needed are easy to find and cheap, maybe most of us already have those on hand, basically need:
a syringe, 2 syringes needles with one thinner (enough to pass trough the larger one) & longer (to make it longer I stick a fine sewing needle that fit at the top of it..) and the other syringe needle larger & shorter (cut), and a spring.



The tip of the larger needle is cut to be flat like a little tube to take the agar sample & a bit bended because if it’s straight the samples will stick to the plate most of the time, if it’s bended after the punch you just need to turn the syringe (1/4 to 1/2 turn) before removing and like this the sample stay in the needle that’s the trick! and after you just need to push on the syringe to transfer the micro-agar sample to a new plate or a LC.


Not pushed


Pushed


Little agar on the tip when starting to push..


Example of the small size of agar sample 


You can see the tinies holes left after you took it..


Little transfer made like this after a bit of colonisation

I hope this little tool can be useful and enjoyable for your myco-experiences!


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Invisiblebigfootscreepyuncl
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Re: V.L micro-agar transfer tool [Re: V.L] * 1
    #27710084 - 03/27/22 01:23 AM (1 year, 9 months ago)

:popcorn: nice


thanks for taking the time to do this!


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Offlinedyel
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Re: V.L micro-agar transfer tool [Re: bigfootscreepyuncl] * 1
    #27710114 - 03/27/22 03:11 AM (1 year, 9 months ago)

interesting twist on the josex poke, what gage are you using for the big needle 16?


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InvisibleSwabMarleyS
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Re: V.L micro-agar transfer tool [Re: dyel] * 2
    #27710117 - 03/27/22 03:17 AM (1 year, 9 months ago)

Nice work bud!
:seriousthumbsup:


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OfflineV.L
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Re: V.L micro-agar transfer tool [Re: dyel] * 1
    #27710120 - 03/27/22 03:52 AM (1 year, 9 months ago)

Quote:

dyel said: what gage are you using for the big needle 16?




Thanks! I think the pink ones are 18g but important is the size of the thinner one compared to the larger one to pass trough it..


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InvisibleBaba Yaga
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Re: V.L micro-agar transfer tool [Re: V.L] * 1
    #27710126 - 03/27/22 04:41 AM (1 year, 9 months ago)

Very cool!

Got all the stuff here to make one.

How long does it take to cool after flaming?


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OfflineV.L
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Re: V.L micro-agar transfer tool [Re: Baba Yaga] * 2
    #27710130 - 03/27/22 04:55 AM (1 year, 9 months ago)

I didn’t check exactly, I should do it with a little timer I would say around 2min, I usually flame it directly after the transfer so during labeling and wrapping time it cool down for the next use and if you work really fast why not build 2 to have one cooling when using the other..


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InvisiblefahtsterM
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Re: V.L micro-agar transfer tool [Re: V.L] * 1
    #27710341 - 03/27/22 09:47 AM (1 year, 9 months ago)

Super neat.. I want one and don’t use agar.  Could use it for cloning to grain tho.  Nice job :headbanger:


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InvisibleDoctor Mario
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Re: V.L micro-agar transfer tool [Re: fahtster] * 1
    #27711239 - 03/28/22 07:03 AM (1 year, 9 months ago)

That's pretty cool. Thanks for sharing.


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Invisiblefungusul
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Re: V.L micro-agar transfer tool [Re: Doctor Mario] * 1
    #27711959 - 03/28/22 07:09 PM (1 year, 9 months ago)

Thanks! :rockon:


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OfflineWeavieWonder
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Re: V.L micro-agar transfer tool [Re: fungusul] * 1
    #27712124 - 03/28/22 09:22 PM (1 year, 9 months ago)

Fucking legit bud. I like the idea of using something like this for inoculating and extracting mycelium from test tubes instead of a scalpel. :takingnotes:

I'll take three.:grin:


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OfflineV.L
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Re: V.L micro-agar transfer tool [Re: WeavieWonder] * 1
    #27712443 - 03/29/22 03:43 AM (1 year, 9 months ago)

Thanks everyone for your enthusiasm! I hope it will be enjoyed if you try to build & use it :smile:


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OfflineV.L
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Re: V.L micro-agar transfer tool [Re: V.L] * 1
    #27728364 - 04/10/22 05:27 AM (1 year, 9 months ago)

As some people here and elsewhere asked what Gauges needles i used I searched for the color code (I ask syringes by colors in the pharmacy) so if we refer to that code should be: larger one pink 18G 1,2mm and the thinner green 21G 0,8mm



(Not all syringes companies use this code)


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Offlinewonderousovoid
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Re: V.L micro-agar transfer tool [Re: V.L]
    #27930478 - 09/02/22 11:27 AM (1 year, 4 months ago)

Sorry if this is a dumb question or if I missed it but how did you get the screw into the plunger like that?

I'm rigging together one of these the only issues I'm having is how to get a screw in there like yours and I can't find a decent spring or needle but I've got them lying around somewhere

Cool idea man thanks for sharing

Edit: nevermind I popped the rubber plug off of one of my syringes. A few syringes I tried the rubber would not come off


Edited by wonderousovoid (09/02/22 11:30 AM)


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Offlinewonderousovoid
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Re: V.L micro-agar transfer tool [Re: wonderousovoid]
    #27932268 - 09/03/22 01:19 PM (1 year, 4 months ago)

How did you end up cutting the syringe needles without mangling them or crimping them? Did you saw em?

I tried cutting one without really thinking about it and just crimped it closed after it was cut, I kinda corrected for it but now the needle is really short. It works but it's shorter than I'd like it to be

I have a few more needles to tinker around with but I don't wanna just destroy em if there's a good way to cut em down


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OfflineV.L
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Re: V.L micro-agar transfer tool [Re: wonderousovoid]
    #27934894 - 09/05/22 07:14 AM (1 year, 4 months ago)

It was crimped after cutting it with cutting pliers but just reopened the hole with a knife or something like that and then used a stone sharpened as finishing to have a clean shape..


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OfflineHappinessStan
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Re: V.L micro-agar transfer tool [Re: V.L]
    #27934968 - 09/05/22 08:17 AM (1 year, 4 months ago)

This is awesome, exactly what I want to make
Many thanks.


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OfflineGrind365
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Re: V.L micro-agar transfer tool [Re: HappinessStan]
    #27935146 - 09/05/22 10:31 AM (1 year, 4 months ago)

Cool


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