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OfflineHongosamongus
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27713099 - 03/29/22 04:06 PM (1 year, 9 months ago)

The syringe method sounds simple enough, but out of curiosity, can this also be done in the PC or will the syringe definitely melt?

I wonder if glass syringes that can take being autoclaved are still around...maybe for veterinary use?


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #27713121 - 03/29/22 04:25 PM (1 year, 9 months ago)

Yes you can sterilize syringes in the PC, at least some brands. I have TemuroTM syringes and they leak after PCing, that's why
I do it with the boiling pot method.Β Look up josex poke and see how he runs them through the PC.


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OfflineSagescruffy
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27713552 - 03/29/22 10:54 PM (1 year, 9 months ago)

@Adrenaline_Drip: those are sexy! Hell yeah!

@ekabpills: those are sexy as well!

Thanks for sharing y’all πŸ™


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Love.


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Offlinemoonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: Sagescruffy] * 2
    #27713628 - 03/30/22 03:37 AM (1 year, 9 months ago)

I'm attempting to fruit pans in a sealed tub with practically no FAE. The 'tub' (ice cream container) contains a tray filled with sodium hydroxide (drain cleaner) to absorb the CO2. Kinda like the CO2 scrubbers used in submarines, but way simpler. Will see what happens. This is the first time I've opened the tray since pinning began 3 days ago. Substrate depth is an inch (too little) and as you can see the casing is drying already from the pins developing, I won't be watering for now as to not risk aborts.



Lighter for scale



Edited by moonwatcher (03/30/22 03:59 AM)


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #27713637 - 03/30/22 04:24 AM (1 year, 9 months ago)

Thanks for posting. This could almost count as a novelty grow.
Well you got fruits, which is more than others have achieved. 

What is the motivation behind this approach, just curiosity?


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Offlinemoonwatcher
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27713758 - 03/30/22 08:02 AM (1 year, 9 months ago)

Quote:

Baba Yaga said:
What is the motivation behind this approach, just curiosity?




I guess so. It could be interesting if cyans can be fruited in a monotub by just eliminating the CO2 without bubblers, foggers and fans on a timer. And the elimination of FAE stops the water loss, so misting not necessary and so on. Would make fruiting a lot easier during the winter. But I'm still waiting to see how well the caps develop and open up and so on.

Also a more interesting way, instead of a CO2 absorber could be to combine the tub with a plant that removes CO2 and re-supplies oxygen. Seems like it wouldn't work, until I did some calculations and saw that a relatively small(5-6 inches) tray planted with corn/maize(a C4 plant) and given a 10w light can easily remove a liter or two of CO2 per day, which is plenty and can keep the CO2 levels inside the tub at even less than ambient CO2 levels (<400 PPM).


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Offlinebunsie
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #27713955 - 03/30/22 11:52 AM (1 year, 9 months ago)

Quote:

Baba Yaga said:
Quote:

bunsie said:
Will hpoo and coir work as a substrate?




Coir and H-manure works great. I use a ratio of 50/50. Make sure to keep the mix fluffy.




So I shouldn't compress the substrate a bit after I put the colonized grains in there?


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher] * 2
    #27713956 - 03/30/22 11:53 AM (1 year, 9 months ago)

Did my first two outdoor bispo beds this week.

The first one I made by dumping a spent tray onto the ground, covering with a layer of manure, and then a layer of straw and watering.

The beds I did today were made by sprinkling freshly colonized grain spawn onto bare earth, covering half the patch with leached but wet manure from my outdoor pile, and half the patch with very dried manure, then covering the whole thing with straw and chicken wire and watering.












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OfflineAdas
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 1
    #27713968 - 03/30/22 12:01 PM (1 year, 9 months ago)

Looking great! I bet they will do well!


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: bunsie]
    #27713992 - 03/30/22 12:22 PM (1 year, 9 months ago)

Yay Hindsight, nicely done.


Quote:

bunsie said:
Quote:

Baba Yaga said:
Quote:

bunsie said:
Will hpoo and coir work as a substrate?




Coir and H-manure works great. I use a ratio of 50/50. Make sure to keep the mix fluffy.




So I shouldn't compress the substrate a bit after I put the colonized grains in there?





By fluffy I mean not soggy / too wet. A little bit of compressing is normal I suppose and shouldn't hurt.


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OfflineSagescruffy
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Re: Official Copelandia/Panaeolus Thread [Re: moonwatcher]
    #27714363 - 03/30/22 04:47 PM (1 year, 9 months ago)

Quote:

moonwatcher said:
I'm attempting to fruit pans in a sealed tub with practically no FAE. The 'tub' (ice cream container) contains a tray filled with sodium hydroxide (drain cleaner) to absorb the CO2. Kinda like the CO2 scrubbers used in submarines, but way simpler. Will see what happens. This is the first time I've opened the tray since pinning began 3 days ago. Substrate depth is an inch (too little) and as you can see the casing is drying already from the pins developing, I won't be watering for now as to not risk aborts.



Lighter for scale






Interesting!


@hindsight: dope! :headbanger:


Bisporus on the left and cyan on the right, ready to be spawned. This is my first time growing these guys out and I was not expecting their myc to look so different. Bisporus reminds me of the sclerotia producers myc before it gets weird



Bisporus is much slower than the cyans. After a shake the bisporus started growing significantly faster. Not sure why, maybe a grain hydration thing? This is the 2nd time I’ve shaken it. First time the growth was slow almost thought it stalled at one point but 2nd time it seems significantly faster. Though more than likely it’s just an β€œillusion”

I’ve shaken both these jars twice because they started out with only a small amount of mycelium. Even after 2 weeks it was just a small circle so I then I shook them to hurry them along and then 2 weeks later one last shake. Just

This is what my FC is going to be. 12 of them filled to about the 250m line. 1qt spawn to 2 quarts substrate (cpoo/verm/coir)



They’ll colonize with the lids on and then to fruit I’ll just stack another container on top



I’m doing this because I’ve had success using these to fruit the truffle producers. Also in my mind this makes it more likely I’ll at least get a print as opposed to an entire tub where maybe it just goes bad




Edited by Sagescruffy (03/30/22 07:32 PM)


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InvisibleSnazz
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Re: Official Copelandia/Panaeolus Thread [Re: bunsie]
    #27714389 - 03/30/22 05:20 PM (1 year, 9 months ago)

Quote:

bunsie said:
Will hpoo and coir work as a substrate?




Coir isn't optimal and may lead to issues.  Chopped straw, poo and verm.  It's picky and needs the aeration and scaffolding that straw provides


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Offlinerockyfungus
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz] * 1
    #27714541 - 03/30/22 07:27 PM (1 year, 9 months ago)

More pans hit the fruiting chamber cased. I don't remember what I dialed in the last few years. Been doing gourmet for a few years.
I figured this would be more intuitive lol.


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OfflineSagescruffy
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Re: Official Copelandia/Panaeolus Thread [Re: rockyfungus]
    #27714547 - 03/30/22 07:33 PM (1 year, 9 months ago)

Quote:

rockyfungus said:
More pans hit the fruiting chamber cased. I don't remember what I dialed in the last few years. Been doing gourmet for a few years.
I figured this would be more intuitive lol.




Dope! Excited to see your fruits!


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Offlinefrankwhite85
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Re: Official Copelandia/Panaeolus Thread [Re: Sagescruffy]
    #27714769 - 03/30/22 11:07 PM (1 year, 9 months ago)

Streaked some plates about a week ago. Peace River Florida Pan cyan on the left and Pan cambo Jamaican on the right. Not used to working with this wispy of mycelium just seeing if these look healthy and where would be good places to take transfers from. 



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Offlinebunsie
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Re: Official Copelandia/Panaeolus Thread [Re: Snazz]
    #27714842 - 03/31/22 12:28 AM (1 year, 9 months ago)

Quote:

Snazz said:
Quote:

bunsie said:
Will hpoo and coir work as a substrate?




Coir isn't optimal and may lead to issues.  Chopped straw, poo and verm.  It's picky and needs the aeration and scaffolding that straw provides




What kind of issues can it lead to?
I heard from Baba Yaga that h-manure/coir will work fine.
I already mixed it into the substrate and slightly watered it.
I'll see if it'll work or not :smile:


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InvisibleBaba Yaga
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox]
    #27714993 - 03/31/22 05:39 AM (1 year, 9 months ago)

Should be fine, as long as the substrate was not too wet when you spawned. I've been using coir/manure or coir/compost exclusively for
the last 5 years with great results. Had a go at straw for the first time last year but it's a pain to prep when starting with a bale so I
stopped using it pretty fast.

I would give pelleted straw a go if I could get my hands on it or pelleted corncobs.

Quote:

bunsie said:
I'll see if it'll work or not :smile:




:thumbup:


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OfflineHindsight
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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #27715114 - 03/31/22 08:01 AM (1 year, 9 months ago)

I'll second that coir/manure works fine. The only catch is that you should not compress the coir. I know a lot of cube growers will pack the coir down quite a bit. That works well for cubes and it suffocates pans. I did a test with several tubs, and the same clone culture from the same plate, divided up into 4 grain jars. Two were coir/manure, one packed and one not packed. Two were the classic straw/verm/manure unpacked. The packed coir/manure tub never even fully consolidated. It just stalled out very early and no mycelium ever made it to the surface.

As long as I don't compress the coir in the tub, I have good luck with it. I find that I have more consistent results with coir, but I get the chance for better results with straw. I believe this is because the pan mycelium prefers straw, but straw is more of a contamination vector so you are playing the odds on straw tubs more than you are on coir tubs. These days I am not doing much with coir and am instead experimenting with straw alternatives like shredded hemp. But again, coir does work and I have plenty of great trays to show for it, as does Baba.


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Offlineshr00mFer
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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 2
    #27715127 - 03/31/22 08:17 AM (1 year, 9 months ago)

Is it better to add some coir in straw/manure/verm mixture? Are there any advantages?

Pan cyan is by far best psilocybe mushroom. After growing some regular ones, had one tub with pe6. But only after growing pan cyan and trying it recently for the first time, I finally got full experience.


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OfflineSagescruffy
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Re: Official Copelandia/Panaeolus Thread [Re: shr00mFer]
    #27715635 - 03/31/22 04:07 PM (1 year, 9 months ago)

Quote:

shr00mFer said:
Is it better to add some coir in straw/manure/verm mixture? Are there any advantages?

Pan cyan is by far best psilocybe mushroom. After growing some regular ones, had one tub with pe6. But only after growing pan cyan and trying it recently for the first time, I finally got full experience.




I just spawned to some substrate that’s mostly cpoo with verm and a little bit of coir for texture. I don’t know if it’s better to add, but a little bit made the texture of my substrate better

Also, update:



These are the pans on cpoo/verm/coir. The 1cup/250ml line is about 1.25”. Thought I had enough spawn and substrate for 12 of these but it ended up being 7. Pretty good number though!

That was my first time smelling pan cyan mycelium. Much different than cubensis


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