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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 1
    #27704511 -

Mr.Giggles said:
Have any of you guys ever noticed pseudo-sclerotia in your bisporus jars or substrate? I'm just curious because my cincts from Warren Co., PA, look a lot like bisporus to me. Idk if there was any DNA testing done on this strain, but they don't really look like the cincts I find around here. The stipes look a lot different, as does the black bands.



Yes actually - in jars. I never said anything about it because I thought people would think I was crazy lol.


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* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 1
    #27704533 -

Mr.Giggles said:
Have any of you guys ever noticed pseudo-sclerotia in your bisporus jars or substrate? I'm just curious because my cincts from Warren Co., PA, look a lot like bisporus to me. Idk if there was any DNA testing done on this strain, but they don't really look like the cincts I find around here. The stipes look a lot different, as does the black bands.








The thousand dollar question is: Do they bruise? If not, no chance of being Bisporus. Other test: Take 100mg dry and see if you feel anything. No chance to feel anything unless it's Bisporus.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 1
    #27704617 -

Bispo millet pint. Good to shake?



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Collected notes on growing Psilocybe natalensis . Collected notes on growing P. subtropicalis

I have P. natalensis, P. mexicana and P. cubensis prints for trade!

Corncob Pellet Trials (ongoing)

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Re: Official Copelandia/Panaeolus Thread [Re: Moopers]
    #27704630 -

Looks great, shake it!

I'd liek to ask you folks if your pasteurized poo substrates start smelling a bit unpleasantly sweet after sitting for a while. Becaue mine always do. This doesn't normally cause problems, but it makes me wonder if it could be the cause of some of my recent failures. Especially with my Bunnell Cyans - recently I can't get them to pin. When I was starting with them, I could get pins in like 3 days or so. My current tub has been cased for like 2 weeks now and still nothing. I'm clueless.

Or maybe my culture just went bad? I went pretty fast through generations but anything is possible?


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Moopers]
    #27704635 -

I vote yes. I might be shaking a little early, but I would have a couple of days ago.

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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27704659 -

Adas said:
Looks great, shake it!

I'd liek to ask you folks if your pasteurized poo substrates start smelling a bit unpleasantly sweet after sitting for a while. Becaue mine always do. This doesn't normally cause problems, but it makes me wonder if it could be the cause of some of my recent failures. Especially with my Bunnell Cyans - recently I can't get them to pin. When I was starting with them, I could get pins in like 3 days or so. My current tub has been cased for like 2 weeks now and still nothing. I'm clueless.

Or maybe my culture just went bad? I went pretty fast through generations but anything is possible?


No I don't notice a difference in smell after aging, though I admit I don't really smell it right out of the cooker because I keep it sealed from then until 3-7 days of aging is done. It always smells bad when I open it, but I will say that it smelled 10000x worse when I used the traditional 2-hour stove pasteurization teks in my early pan days, vs the 12 hour overnight instapot. It still smells bad but it's tolerable (the stove tek had it smelling like the worst sulfur sewer ever). Once colonized, it has a sweeter smell to it but I wouldn't say it's VERY sweet. Just not as nasty with some overtones of what a grain jar smells like (which is a tiny bit sweet).


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


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Re: Official Copelandia/Panaeolus Thread [Re: Hindsight]
    #27704675 -

I didnt mean strongly sweet, just.. a bit sweet and unpleasant :laugh: In any case, the only thing that ever refused to colonize it altogether was Zapotecorum. Everything else did okay at least, though Cubes and Nats seemed to struggle with bacteria or something.


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27704812 -

Hey Mr giggles i will also suggest to try it on you self. Cinctulus should be around same strength as cubes the bisp 3-4x stronger. But do a half gram for science. :crazy2:


My input on pasteurization is when i started reading this thread at page 350 the hype was vacuum-bags. But you need to open it after cooking to avoid it starts to smell that funky smell. It's seams to need fresh air.

I use 1L. Glasses with poly filter. I pack the poo in the bottom and think it helps with the smell. I do 30/70 poo/straw but have experimented with some wood chips, coffee, coir, vermiculite, lime,grass seeds, hydrat lime, and gypsum but not enough to say something. I give it 2 houres at 70 c. In the destilator and happily use it as soon it is colde. If i save a day or to i do it ind My refrigerator. I dig it out of the glass with a fork to a 8L. freezer bag mix it with spawn put a poly filter on and let it colonize in the bag.


My destilator and carbon filter.



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Re: Official Copelandia/Panaeolus Thread [Re: Adas]
    #27704889 -

Adas - bluing at the base was common and I specifically remember cutting/breaking stipes and would bruise blue or purple. I believe Subfinder said they were some of the most potent cinctulus he had ever tried. I purchased a spore syringe from an online vendor tho, it was my first grow along with some cubes. It does some strange stuff. I remember one tray producing fruits with very striated caps. I thought it was psilocybe mexicana when I first noticed them. I also recall a shoebox first showing pins and they were completely different looking from the typical banded orange-brown cinctulus pins, they were all a plain light to dark brown. I thought I had maybe mislabeled a shoebox of cyanescens I had going but I didn't. Only a couple people in the Blue Pearl Explorer's Thread posted pics of fruits if memory serves me right, probably some of you guys lol but man it was difficult to type this on 3g of cubes, took hours lol I think? Trying to remember when I ate those...


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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH]
    #27704917 -

Mycosmyth - I have some on agar right now so it'll be a little while. When I tried them I was prescribed to an insane amount of gabapentin (3200mg/day) which broke my brain and I think blocked psilocybin. Example: I was with two people who had eaten 2g of the Ban Hua Thanon I had grown and they were having a good time. Meanwhile I ate like 3g of those and another 3g of the cincts and all that happened was anxiety along with stomach and back pain... I laid down and slept it off. As I've been tapering off gabapentin I've noticed mushrooms affect me more. That crap fucked with my memory soooo bad. It's really hard to get off of it. Idk what my doc was thinking putting someone fresh in sobriety onto such an addictive drug... but I'm rambling and tripping and starting to feel negative so I'm going to smoke some herb and chill for a bit ✌


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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles]
    #27705220 -

Ok Mr giggles hang on to life. I have never see cinctulus or bisp in real life but seen relly many pic on the net. I have Noticed that cinctulus look more red/Brown and bisp looks more gray.

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Re: Official Copelandia/Panaeolus Thread [Re: THE MYCOSMITH] * 6
    #27705336 -

Hi, MrGiggle I did both few time indoor and observed them also in the garden for me they look more Pan bispo (the dark grey ring) but as others said if it’s blueing and very potent as cyan should be bispo, compared cinctulus are weak...
Few pics to help you to make an idea (often image speak better than words..)

Pan cinctulus:
Pan cinctulus
Indoor cinctulus
Indoor Pan cinctulus
[/url] Wild Pan cinctulus from my strawberry bag
Pan cinctulus myc


Pan bisporus:
Pan bispo indoor
Pan bispo



--------------------
  Exotics Fanaticus cakes: EF tek

  Micro-Agar transfer tool

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Re: Official Copelandia/Panaeolus Thread [Re: V.L]
    #27705388 -

I'm beginning to think they're definitely bisporus. I could try to use microscopy, just starting out. I remember Subfinder saying they were some of the most potent cinctulus he'd ever found, Also, I found a hidden sheet of prints today if anyone wants one.
Note - These were from a vendor, could potentially be a mix-up.


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Edited by Mr.Giggles (03/23/22 06:47 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 4
    #27705694 -

I felt like the grow had shown me what it had so I pulled the rest and shut it down on the 19th.
The last fruit were better than my first round, but there wasn't really anything there I wanted to clone.
Ended up with a nice little jars worth, enough for a fun evening or two, I didn't weight cos I'm lazy!

The clones I made from the early fruit are looking clean which is exciting! Still haven't found an expression of this print that I like. I'll see how the clones work out and maybe revisit it again. Next I'm going to finally grow some tamps. I've streaked them three times and never got passed agar cos life throws me a curve ball. Fingers crossed this time it'll work out.




:carlton:


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Official Copelandia/Panaeolus Thread [Re: coversall] * 2
    #27705734 -

Fuckin hell Coversall those shrooms are freaks!


--------------------
Shrooms are always bluer on the other side...

Work hard, play harder, trip the hardest.

Simple Cloning TEK by Adas
Semi-Sterile TEK (SS-TEK)
Growing Panaeolus bisporus - The Low-Tech approach
Exotic Mycelia Surviving Winter - Creating IceBreaker Variants!
My First Panaeolus Cyanescens Grow!
Psilocybe azurescens photo compilation 2020
Azurescens in June!

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Re: Official Copelandia/Panaeolus Thread [Re: Adas] * 2
    #27706368 -

I checked a couple agar cups I had from my wild cinct clones and found some of these:




I transfered a few. Gotta make more agar. Also this old jar of cyans is doing strange things. Not sure if it's bruised mycelium or if there are little sclerotia under there. I wonder how many species produce it 🤔



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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 1
    #27706378 -

Gnarly looking!

The Peace River Florida I had on agar looked a lot like the 2nd photo. In addition to the blobs under the surface, it also formed knots on the surface that bruised, but never grew into pins.

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Re: Official Copelandia/Panaeolus Thread [Re: ruawakeyet] * 1
    #27706404 -

Yeah they're cool! The big one was only about the size of a hydrated rye seed. I'm going to keep this cinctulus strain going and try to select for sclerotia. It will probably take many generations I'm guessing. I have seen photos of large blue pearls before so it's possible. I had one agar jar that I put to grain a couple hours ago.


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Re: Official Copelandia/Panaeolus Thread [Re: Mr.Giggles] * 5
    #27706560 -

First wave of Pan Cyan Texas - long-legged creatures )):

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Re: Official Copelandia/Panaeolus Thread [Re: Mico Zapotec] * 3
    #27706577 -

ekabpils said:
First wave of Pan Cyan Texas - long-legged creatures )):



Rad! The texans have always been a solid fruiter for me! Looks like you've done a great job with them!


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..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Shop: Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co Certified Organic All-In-One Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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