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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThurmanMurman] * 1
    #28889747 -

ThurmanMurman said:
Thats good to hear. Thanks for helping me check that off of my troubleshooting list.


ill be honest, i actually think condensated plates last longer.  i send every plate on the wall so its a non issue for me but i really wouldnt worry about that.

spores can take a real long time.  i remember a gandalf plate that took well over a month.  totally forgot about it then i just noticed it had myc one day


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: BeefExtreme]
    #28889758 -

I didnt really worry about it until I had pooling on the agar itself. And I've heard spores can take a while, but I'm basically waiting on this to kick start things done "properly" while I do super sketchy shit in the meantime just bc I cant sit still. The beginning stage is sooo tedious.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThurmanMurman]
    #28889833 -

how long has it been since you streaked?


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: BeefExtreme]
    #28889939 -

5 plates on the 12th and 5 plates the 20th. Im less concerned with the latter, but over 2 weeks without germination has me worried.


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Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
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Shine until the two become one

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ThurmanMurman] * 1
    #28893170 -

ThurmanMurman said:
I didnt really worry about it until I had pooling on the agar itself. And I've heard spores can take a while, but I'm basically waiting on this to kick start things done "properly" while I do super sketchy shit in the meantime just bc I cant sit still. The beginning stage is sooo tedious.


Just lightly dump the pooling water out when you're about to streak spores or transfer.


BeefSupremeJr said:
how long has it been since you streaked?


Not since that weekend he got banned from Wrigley Field.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: stareatclouds] * 1
    #28893240 -

The pooling is sterile, so if you're attempting to streak dry spores from a print it can aid in spreading. :cool:

I always prep a syringe of sterile water to add a drop for streaking (I prefer cell spreader), obviously not necessary when using ms syringe.





Makes for even distribution and separation from contaminants.:thumbup:

Some spores are cleaner than others :lol:

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: BeefExtreme]
    #28893824 -

I had a solid calculator for different agar consistencies that'd factor in the vessel, too. It was helpful having it output how much agar, LME, and water I'd need for 8 plastic slants, a sleeve of Petri dishes, 5 Pasty Plates for transfer and 5 for germination from a print. A damn shame my laptop crashed and my super detailed Myco tracker went with it. C'est la vie.


BeefSupremeJr said:
ill be honest, i actually think condensated plates last longer.  i send every plate on the wall so its a non issue for me but i really wouldnt worry about that.

spores can take a real long time.  i remember a gandalf plate that took well over a month.  totally forgot about it then i just noticed it had myc one day


Not drying out is a benefit, for sure. As they say, moisture is the essence of wetness.


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ATTACK OF THE KILLER APE!!!11 | G2G Video | Stare Sticks | Bags in SAB |
DMT + Laser = Reveals Alien Code Commonly Reported In Trips | JavaScript I Use To Make Shroomery Better [New]

Edited by stareatclouds (08/02/24 01:40 AM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: stareatclouds]
    #28896444 -

What TEK(s) would you recommend for cultivating several small projects simultaneously? I have 6+ different cubensis varieties that I want to bring to fruit. I would like keep a small(er) footprint and I don't care about producing any weight. PF? Fahtlocs? I'd prefer a solution I can implement in my tent, but am open to all recommendations


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LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

Edited by mycosispeon (08/01/24 02:28 PM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: mycosispeon]
    #28896577 -

In your tent?
That sounds amazing.
Personally in a tent Fahtlocs would be my personal choice.


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Prior to first flush cotton ball like growth.... [Re: Munchauzen]
    #28896711 -







So I posted this question under recognizing contamination before I found this thread but will update that thread if I get an answer here for posterity and all that. 

This is my second attempt and I have no idea if this is mold or is it mycelium growing up due to conditions.  I found a thread that sounds like the same thing but the pictures have been deleted so I can't confirm.

Conditions: After colonization and transferred into tub, a steady 73.5 F with 95% humidity with adequate air exchange, light 12 hours a day.

Everything has been going well...then today I noticed a bunch of these pure white cotton ball like things growing (no color or funny smell).  They seem to have structure and don't look like the pictures of cobweb mold to my eyes but being a noob I don't trust my own eyes. 

Help please! 

My questions are:
  • Is this something normal(ish) and if so is there something I should change (air, humidity, temp, other magic)? 
  • If it's fucked can it be saved (and how)? 
  • Bonus: Help ease a generally anxious person and explain what the hell I'm looking at?!


Totally appreciate any help (and if it's fucked general sympathy) also any feedback on what ye trusted eyes see!

Thanks.

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Re: Prior to first flush cotton ball like growth.... [Re: Parkerg]
    #28923000 -

How long do your AA sterilizers hold vacuum for?  Mine seems to be effective and hold for only a few hours.  If it's been over 12 hours I can't hear it pull a vacuum when opening the valve.

I've ensured the seat within the valve is clean, ptfe'd the threads and use vacuum grease on metal seal

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: mycosispeon] * 1
    #28957243 -

mycosispeon said:
What TEK(s) would you recommend for cultivating several small projects simultaneously? I have 6+ different cubensis varieties that I want to bring to fruit. I would like keep a small(er) footprint and I don't care about producing any weight. PF? Fahtlocs? I'd prefer a solution I can implement in my tent, but am open to all recommendations


I'm a fan of fahtlocs for small projects.  Pretty easy to hold small individual environments in the sweet spot with those giant ziplocks.


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New to cultivation or the shroomery?  Observe the hitchhiker's guide

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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Prior to first flush cotton ball like growth.... [Re: dcell]
    #28957363 -

dcell said:
How long do your AA sterilizers hold vacuum for?  Mine seems to be effective and hold for only a few hours.  If it's been over 12 hours I can't hear it pull a vacuum when opening the valve.

I've ensured the seat within the valve is clean, ptfe'd the threads and use vacuum grease on metal seal


How old is your safety plug? My AA holds a vacuum indefinitely.


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Re: Prior to first flush cotton ball like growth.... [Re: thirdeyewild] * 2
    #28957470 -

Mine too.

The important question is how old is your butt plug and have you washed it since it's last use?


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Re: Prior to first flush cotton ball like growth.... [Re: Nichrome]
    #28957475 -

:rofl:


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Mr Piggy] * 1
    #28958723 -

Mr Piggy said:
mycosispeon said:
What TEK(s) would you recommend for cultivating several small projects simultaneously? I have 6+ different cubensis varieties that I want to bring to fruit. I would like keep a small(er) footprint and I don't care about producing any weight. PF? Fahtlocs? I'd prefer a solution I can implement in my tent, but am open to all recommendations


I'm a fan of fahtlocs for small projects.  Pretty easy to hold small individual environments in the sweet spot with those giant ziplocks.


Yeah. Definitely going to go this route. :thanx:


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LAGM 2.024



The Hitchhiker's Guide to the Shroomery | Pastywhyte’s Ez dialed monotubs | Clean spawn checklist for the new grower | How I do Rye Berry Prep | Frank's Simple Coir/Verm Tek | Stro's Cleaning and Isolating On Agar

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Munchauzen]
    #29001823 -

Can you make colonized cakes from coir and grain and use the same teks as for BRF cakes, provided that you use proven axenic inoculant only?


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Arctic_Fox] * 2
    #29001906 -

You can, but they probably need to be pressure cooked. I know a lot of people steam PF cakes but that might not be enough for grain.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: A.k.a] * 1
    #29001926 -

:whathesaid:
Look into muda bottles


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: tryptkaloids]
    #29001940 -

Yup, grain must be PCed


--------------------
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New to cultivation or the shroomery?  Observe the hitchhiker's guide

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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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