Stipe-n Cap said:

I learned this from Bill Burr and have been applying cream to more than just my junk for ages.

Bill Burr tek is how I maintain my youth

---

SirPsycho said:

Stipe-n Cap said:

Id be more worried about my skin while the lids open. Skin sheds particles.

---

You need to moisturize. Cream isn't only for your dick. You need to increase your surface area of application. You ever wonder why your dick looks 20 years younger and you look like an old catchers mitt?

---

Stipe-n Cap said:

I learned this from Bill Burr and have been applying cream to more than just my junk for ages.

Bill Burr tek is how I maintain my youth

---

what you do with a plate that got mold?

like plate is 90% colonize and at the edge green mold appear its still white didnt turn green yet?

start over? or cut it out?

MicroMycelium said:

Noticed a few of my freshly poured agar plates had a spec of bacteria growing on the rim where the agar meets the glass.

---

nektar61 said:

MicroMycelium said:

Noticed a few of my freshly poured agar plates had a spec of bacteria growing on the rim where the agar meets the glass.

---

I had this problem with glass plates, until I moved to disposable sterile plastic plates. Glass plates I had didn't quite fit together at all points around the circle as well as plastic ones too.

---

pesa said:

thing about glass petri-dish is you need to tightly pack it with parafilm or pvc film, its meant to have a gap, that gap is used when you pour agar to let the excess moisture gases escape, otherwise it in autoclave the glass petri-dish accidentally wet and there is no way for steam to escape it will explode.

---

MicroMycelium said:

pesa said:

thing about glass petri-dish is you need to tightly pack it with parafilm or pvc film, its meant to have a gap, that gap is used when you pour agar to let the excess moisture gases escape, otherwise it in autoclave the glass petri-dish accidentally wet and there is no way for steam to escape it will explode.

---

Do you have a specific way of storing your blank/unused plates before inoculating them? I parafilm mine once being used, but i assume that gap is what allowed contams to get in them during the few days I left the blank ones on my shelf in my grow tent.
I’m confident it wasn’t the agar because the contamination would of been on all of them but the ones I sealed with parafilm immediately were fine.

---

once you pour agar and it settles
dish are ready to use, if you don't cover them with paraflim contam's will take over.
you have to make sure all of them use and unused are cover with paraflim. otherwise contam from air will enter and colonize.

i keep mine unused store in airtight bucket like this all of them are with pvc film(join the pvc film gang)
all are glass and cover with pvc film

Tri-Polar said:

just wrap your plates once cooled, I think stipe has a thing about wrapping wholes sleeves of unused plates at a time with clingwrap

---