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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28383033 -



Tried out the Maltoferm 1001, had already tried the CBW golden light and from a chart I had found the difference between the two was that the maltoferm is food grade while the cbw is brewers grade.

Having trouble finding that chart but by any means, did 2% nutes 1.375% agar, following the table mycolorado posted. Definitely the clearest unstrained agar I've had to date, very stoked to get rid of the boogers :biggrin:

Wondering if the grade is responsible for the hot break somehow as the brewers grade is supposed to be higher quality, so its not a quality thing in a bad way lol


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28383059 -

Arctic_Fox said:
I will note that petris can be difficult to pick up. Is there a technique to this? When trying to pick up a closed petri dish, my gloves invariably get under the edge (of course they do, it's the only way to grasp the bottom under the cover) and this can't be good for sterility. I imagine that I introduce contamination under the lip of the cover and then airflow from the flow hood blows them into the media.


Did anyone have any thoughts on this?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 1
    #28383064 -

Slide to the edge of the bench in front of the hood and pick up.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 1
    #28383065 -

Arctic_Fox said:
Arctic_Fox said:
I will note that petris can be difficult to pick up. Is there a technique to this? When trying to pick up a closed petri dish, my gloves invariably get under the edge (of course they do, it's the only way to grasp the bottom under the cover) and this can't be good for sterility. I imagine that I introduce contamination under the lip of the cover and then airflow from the flow hood blows them into the media.


Did anyone have any thoughts on this?


I am wearing gloves I've sprayed with ISO alcohol (I think everyone does).

I re-spray them a few times during a session if I'm doing a lot. I spray into the room, not onto my work area. (though I spray and wipe my work area before I start).

I spray both sides of one glove, set the alcohol down and rub my hands together to get alcohol all over both gloves.

I hold my hands up (not over the work) and dry them with the flowhood after I re-spray, and never use the flame torch while I'm wet or even just after they've dried. (If you're using an SAB, never use flame inside it).

I pick the lid up with my fingers down (like a claw machine), then gently flip my hand over so the lid is upside down, and kind of toss it onto my work surface.

I make sure I don't pass my hand over the inside of it or especially over the open plate.

To put it back on, I reach under it, palm up, grasp it, flip my hand over again like the claw and place it on the dish. Then use grating tape to seal it, a few times around, then one time around with micropore tape, gently squeeze the tape shut around it to close any gaps.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #28383088 -

Doesn't sealing it involve picking up the whole thing and not just the lid?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28383141 -

You can try the celltreat gripring petris - there is a slightly pronouned ring on the bottom plate that the lid rests on.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28383314 -

You can also ditch the gloves and no need to constantly soak everything in alcohol. You can wipe down your surface with it, run the hood for a few min before working and wash your hands well and you’re golden.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28383360 -

With or without gloves I'm going to be getting my fingers under the lip of the cover to pick up the bottom. No good. But I like your "slide it to the edge of the table" idea.

And here's a photo of one of my plates. You can see where I streaked it (looks like a "Z I"), but something is growing in between the streaks, so would I be correct in assuming that that is contamination?



I'm still trying to figure out how to take clear photos. I tried a white paper background but the contrast was actually worse.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 1
    #28383365 -

It looks satellite mold from hovering. If you check out the why agar link in my sig, I have yt vids showing how to properly streak.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28383367 -

:whathesaid:
No need to tear the agar when you streak….gently slide your loop across the surface.  If you didn’t streak in that spot then yes, it’s likely a contam.  The technique I described is fool proof.


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Edited by Mycolorado (07/03/23 09:16 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28383379 -

All my plates have stuff growing where they shouldn't be. I might nuke them all and start over with better technique.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 1
    #28383381 -

:nodofunderstanding:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28383392 -

I don’t glove up and don’t worry too much about where my fingers touch the lid, since the lip isn’t going to touch the agar. It might be an air quality problem?


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Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28383407 -

Id be more worried about my skin while the lids open. Skin sheds particles.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28383412 -

Stipe-n Cap said:
Id be more worried about my skin while the lids open. Skin sheds particles.


πŸ‘†

Where are you keeping your hands when you do work?


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28383423 -

I keep my hands well away from open vessels, but plenty of other's don't. My tools are long, which prevents hovering, but I use a hood which creates turbulence around tools and vessels which could carry particles into your work.

I hate to always be that guy but I'm a stickler for proper tools and techniques. Lab techs wear gloves for a reason, you'll never see one of them raw dogging their work.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28383425 -

I get contams like every 500 plates.


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28383428 -

Stipe-n Cap said:
Id be more worried about my skin while the lids open. Skin sheds particles.


You need to moisturize. Cream isn't only for your dick. You need to increase your surface area of application. You ever wonder why your dick looks 20 years younger and you look like an old catchers mitt?:rofl:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28383429 -

:whathesaid:

When using proper tools/techniques, combined with experience. Satellite or user error contamination becomes extremely rare.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 2
    #28383431 -

I learned this from Bill Burr and have been applying cream to more than just my junk for ages. :rofl:

Bill Burr tek is how I maintain my youth:yields:


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