Funguy50 said:

SirPsycho said:

Blank or inoculated?

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Blank for now but knowing for both would be good.

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MonoVision said:

So I’ve got these plate pins(plins) or whatever you want to call them. Since these are sealed in the dish they should theoretically be sterile, correct?  Should I still split down the middle and take a sample from the middle to clone? Or can I just cut a section off and throw it on a new plate and call it a day?

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Transfer the entire pin and then transfer the mycelium that's grows from the new pin plate.

MonoVision said:

Awesome! Thanks y’all

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To clone a plin, slice the plin above the bottom of its little stem to avoid getting any non-clone mycelium from the agar with it if you want a clone only, which you probably do. Do it with a plate where no plins have dropped spores yet. This is the easiest way to clone, and less likely to have contam than cutting from a shroom growing in the open air.

Then use your scalpel to push it into a clean plate.

Plin pushed into a fresh plate

Everything I've grown in the past two years has been the fruit of a single plate plin of x7x which produced consistently strong shrooms, or of a tall pheno line off that. I lucked out, was my first try, and was stronger than what it grew from.

But the theory is that first plins are often robust genetics since they grow out of sub-ideal conditions and wave their little flags around first. As in "the force is strong in this one." 

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Plins aka plate pins ready to be cloned to a fresh plate.

Tri-Polar said:

Exposed to open air/ AC blowing on them for ~30 seconds at 2% 1% and .2% nutes.

Not feeling warm and fuzzy about nothing showing up at all yet, been having shit luck with agar and im thinking I might need to lower the agar concentration from 2%

Any words of wisdom?

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Tri-Polar said:

Oh shoot thought I mentioned, been 4.5 days

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Tri-Polar said:

Exposed to open air/ AC blowing on them for ~30 seconds at 2% 1% and .2% nutes.

Not feeling warm and fuzzy about nothing showing up at all yet, been having shit luck with agar and im thinking I might need to lower the agar concentration from 2%

Any words of wisdom?

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Tri-Polar said:

Oh shoot thought I mentioned, been 4.5 days, and I have an insane sporeload(moldy apartment building + pnw) so I know shits on there.

Good writeup, I am personally somewhat partial to MEA even though I've been fucking it up a lot recently lol, im drawn to toxic relationships 

I read a thread (the "no pours suck" one) and it seemed like the OP had a lot of contams that weren't showing up due to seemingly his agar being too hard, lowered it to 1.25% or something and found so much trouble with contam I think they denounced SABs and quit the hobby lmao, rough stuff. But from that thread I've been inspired to 1. Lower my agar concentration and see if im secretly harboring nasties and 2. Create a time-lapse of particulates in an SAB

Also my sig is TW and Hillbilly pumpkin

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