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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28384994 -

Stipe-n Cap said:
I have always sterilized agar for 45 mins at around 18psi. The sterilization charts for agar can exceed 45 mins @ 15psi, don't be ridiculous.


every time i go to 45 mins i got more dark burnt agar and nothing grows on it:shrug:

EDIT:also mine is PDA

Edited by pesa (07/04/23 03:02 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28385002 -

You have no idea what you're talking about, and I am most certainly in possession of those charts. Are you trolling me or what?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385003 -

dude i am not, let me dig and show it you.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28385005 -

pesa said:
agar pressure cook time is 25 minutes you can not go beyond that or it will be over cooked agar.
same goes for grains 90 minutes or 120 minutes go beyond that you will end up with burnt grain.

i guess stipe-n cap has that chart thing with agar times on it.


No idea what kind of atom bomb pressure cooker you have, but ive sterilized grains jars for over 3-4 hrs before. Came out fine and unburnt... How is the grain burning if theres moisture in the PC


--------------------
A Handy Dandy Rule Book for the Beginner Cultivator /WBS flash prep Tek /Bulk Substrate Coir Tek /Shoebox Tek /Bottle Tek /Clean spawn Checklist /Agar Recipe /Don't put Spores to Grain /Updated Pf Tek /Surface Conditions


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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa] * 2
    #28385006 -

Here:



https://consteril.com/steam-sterilization-cycles-part-2-liquids/



Agar cycle times are volume dependent. Not only that but I have sterilized agar, first hand experience, at above 121°C for longer than 45 mins and have never once in all of my years experienced evwn a single problem, ever.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385021 -

Stipe-n Cap said:
Just so you're all aware, sterilization times are volume dependent:




this thing liquid quantity and time

250ml 30 minutes, i did PDA 250ml for 45 minutes nothing grew on it

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pandaskis]
    #28385027 -

ok
Pandaskis said:
pesa said:
agar pressure cook time is 25 minutes you can not go beyond that or it will be over cooked agar.
same goes for grains 90 minutes or 120 minutes go beyond that you will end up with burnt grain.

i guess stipe-n cap has that chart thing with agar times on it.


No idea what kind of atom bomb pressure cooker you have, but ive sterilized grains jars for over 3-4 hrs before. Came out fine and unburnt... How is the grain burning if theres moisture in the PC


okay at this point its a question, i got 13psi i follow the cook times according to tables i get burnt agar and burnt grain:shrug:

i can wish for presto but this is what i got

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28385030 -

Like I said. Only noobs have this problem. I have been reading posts on this board for some time now, I have never once even heard of such a thing as a dead plate until like 6 months ago.

I have never heard of a single reputable cultivator ever even hint at something like this being possible. I have never personally experienced, nor have I read about it in published papers. It is a completely new phenomenon experienced by like 4 noobs, so far as I can tell.

I'd love for another TC or someone with a registration date older than 5 years to correct me, but I highly doubt that will happen.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385034 -

try with PDA shoot for over time.i  can not say for other agars i never used them, and i am not talking about potato flakes i am talking about potato extract, dextrose.

in my case its not a dead plate, it becomes like water agar. growth is too slow and on some paltes weeks after nothing grow.

dude we are all noobs reporting something maybe you investigate

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28385036 -

Sure, perhaps there's something special with PDA that I'm unaware of, I've never used it. I have a pretty firm grasp of the underlying mechanism which govern these basic techniques and would be very surprised if water agar could produce growth, but some magical chemical reaction at 121°C rendered your agar completely and irrevocably sterile.

PDA or otherwise.

If such a reaction does exist it will take more than (no offense intended) more than a handful of noobs to convince me.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa] * 4
    #28385041 -

Don’t be ridiculous everyone who experiences this! Just because it happens to you doesn’t mean it’s possible.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: pesa]
    #28385046 -

45mins at 15 PSI after 15min vent, volume around 20ml each plate (I pour 24 plates from 500ml with a bit of leftover). Breiss Pilsen Light Dry Malt Extract (lovinond 2) at 2% by weight. Plate waved around my living room at head level.



--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28385047 -

Sure, but like I said previously, months ago:

Correlation ≠ causation.

There must be something else at play here because 121°C isn't all that hot, 45 minutes isn't so long as to not only completely denature every available protien, it's denatured to the point where life has become impossible.

This should be self evident to anyone who thinks about this problem for a second. There is no causal relationship. There is a lack of historical evidence, or experience by capable cultivators.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28385049 -

Lmao yeah, if my mold plate doesn't grow out there's a problem. Hope it does.

I know I posted this already but relevant


I know the food coloring is a variable, and all my plates now have none. But that pic is a tissue clone to water agar after 6 weeks


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘
TekDeck    Fest-list '25    Autist Tally :laugh:

"Mother Mycelium, I wandered in the darkness of unmarked plates and forgotten codes. Enlighten me with clarity and discipline, that every jar and dish shall bear its true name."

"Luminous Logos, I faltered in my shorthand faith. Grant me the diligence to record in full, and the foresight to recall every meaning with certainty."

-Father LewDoja

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385051 -

Weird, looks like life.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28385052 -

SirPsycho said:
45mins at 15 PSI after 15min vent, volume around 20ml each plate (I pour 24 plates from 500ml with a bit of leftover). Breiss Pilsen Light Dry Malt Extract (lovinond 2) at 2% by weight. Plate waved around my living room at head level.




Thats literally all I want :psycrankey:


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘
TekDeck    Fest-list '25    Autist Tally :laugh:

"Mother Mycelium, I wandered in the darkness of unmarked plates and forgotten codes. Enlighten me with clarity and discipline, that every jar and dish shall bear its true name."

"Luminous Logos, I faltered in my shorthand faith. Grant me the diligence to record in full, and the foresight to recall every meaning with certainty."

-Father LewDoja

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385053 -

Stipe-n Cap said:
Weird, looks like life.


Oh I could be misunderstanding water agar, I thought the mycelium was supposed to spread, not just engulf the tissue sample


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘
TekDeck    Fest-list '25    Autist Tally :laugh:

"Mother Mycelium, I wandered in the darkness of unmarked plates and forgotten codes. Enlighten me with clarity and discipline, that every jar and dish shall bear its true name."

"Luminous Logos, I faltered in my shorthand faith. Grant me the diligence to record in full, and the foresight to recall every meaning with certainty."

-Father LewDoja

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385055 -

Ok I need more from you. I need an alternate explanation why changing that single factor made my plates functional.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 2
    #28385056 -

Dis you inoculate that plate with whats under that thumbnail, you dirty bastard :rofl:

Smellyhobbit said:
Ok I need more from you. I need an alternate explanation why changing that single factor made my plates functional.


I have no idea what the hell is happening. What I do know is that the claim that agar becomes a growth inhibitory substance after 20 mins in the autoclave is fully retarded.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28385059 -

Stipe-n Cap said:
Sure, perhaps there's something special with PDA that I'm unaware of, I've never used it. I have a pretty firm grasp of the underlying mechanism which govern these basic techniques and would be very surprised if water agar could produce growth, but some magical chemical reaction at 121°C rendered your agar completely and irrevocably sterile.

PDA or otherwise.

If such a reaction does exist it will take more than (no offense intended) more than a handful of noobs to convince me.


i try a lot of times to get out of PDA for very reason not much info is available plus i been using your table for very reason i am getting result at 25 minutes time for 250ml
i talked with a lab, they told me this part, time and agar medium and not to over shoot potato extract.:shrug: the info i posted this out of a lab that sold me dextrose

dude i am computer engineer by profession not claiming or ever claim to know it all, all i got is info and pipe it through shroomery. 

when i had problem with seed burst i straight forward came to you for very reason TC.:shrug:

no offense taken, i just wanna figure out da heck is wrong PDA, out side of NORTH AMERICA, things are not according to shroomery tek, sourcing things is not easy amazon outside of NORTH AMERICA does not carry same products.

yeast is not easy to get, i try the brewery once for malt extract didnt went well. so i been using PDA for three years now.

Edited by pesa (07/04/23 03:39 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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