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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Spawning a Cloned Abort * 2
    #28459266 - 09/05/23 08:53 AM (4 months, 20 days ago)

Out of excitement I harvest the fastest growing/first formed pin on my first ever multispore shoeboxes grow(log in my sig).

Here is said pin 19 days after STB:
It was first to form, growing faster and larger than anything else.


This was 20 days after STB:

The responses I got were mixed. Some gave support because of its speed, but others claimed it was an abort due to the cap opening before maturity.

Clone Harvested:


Tissue Germination:
- The fruit was split in half in my SAB and an internal tissue sample taken from the stipe via scalpel.

Transfers:
- Tissue sample was place on a MYA plate and allowed to germinate across roughly 70% of the plate.
- First agar plate looked like trash so transferred sectors from the T0 to a few water agar and MYA T1 plates.

Grains:
- The most uniform, but very tomentose, plate was transferred agar to WBS grain in my SAB.
- Grain jar was shaken at 30%, 70%, 95%.
- Grain to grain transfer was done to 2 more WBS quart jars in my SAB at the 95% mark
- Original jar recovered in about 2~ days, and the receiving jars fully colonized in about a week with one shake at the 50% mark 4 days in.

Spawning: Performed 09/05 evening.
- 3.5qt~ colonized WBS was shaken and poured into a 12qt shoebox.
- 2.5qt~ CVG was poured into the tub
- Substrate was hand mixed until large homogeneous
- Substrate was leveled and compressed with decent force using a stainless burger press tool
- 1qt~ CVG pseudo casing was poured on top of the substrate, leveled and compacted again. (Why this method)

The Tub:

- Note that these are 12qt, wider for more surface area, and have 5 predrilled holes on each side which help with FAE.
- Micropore tape will be used on the holes if I notice the sub gets too dry however my last run with one of these with taped holes lead to stagnant water on the surface and contamination so they will likely be left untouched.
24 Hours after STB:



48 Hours after STB:



72 Hours after STB:


96 Hours after STB:



5 Days after STB:




6 Days after STB:
Surface conditions are looking very damp.. going to dab a paper towel on it to get some of this stagnant water off.



7 Days after STB:


It looks like dabbing up all the stagnant water was a smart move. Surface conditions returned back to small microbeads.

8 Days after STB:


You can see in  the top right quarter surface is developing primordia in the faster-growing areas.
While that’s exciting, I’m actually a little annoyed with the appearance of surface. I spawned a clone and put a flat/compressed pseudo-casing.. I was expecting the combination of those things to create a more consistent/even colonization of the substrate’s surface.
The fact that the surface has not entirely colonized yet but pins are forming makes me a little worried the may be some hiding contamination… or perhaps I just did a crappy job with the leveling/casing and it’s thinner in the faster-colonizing areas.:shrug:

10 Days after STB:


If you look closely at the bottom right, we have our first pin formed.
Turns out, you can clone with these type of genetics. I’m excited to see how it turns out.
I’m a little let down at the lack of an evenly-developed pinset however.

11 Days after STB:



And we have pins all over the surface and multiple clusters forming. It will be interesting to see how these make sure as the abort I picked for a clone was chosen due to the speed of matured rather than the physical characteristics which never got to fully developed due to it well… aborting.

12 days after STB:



14 days after STB:
- First flush covering the entire surface in literally just two weeks.
1. That’s awesome.
2. You can clone an abort fyi.




Edited by MicroMycelium (09/20/23 10:50 PM)


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OfflineLewDoja
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Registered: 09/18/09
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Re: Spawning a Cloned Abort [Re: MicroMycelium] * 1
    #28459464 - 09/05/23 11:46 AM (4 months, 20 days ago)

The only remark I have regarding the methodology is:
If using WA to clean up a culture- it is advisable to put to back on a nutritional medium for testing (any hidden contams that weren't able to show their ugly faces on WA).

Good luck


--------------------
a wise man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



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if we have any pending trades or you never received anything that you were expecting send a PM with details.  I've had a lot going on, and may have overlooked something as well as USPS snafu's.


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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Spawning a Cloned Abort [Re: LewDoja]
    #28459516 - 09/05/23 12:58 PM (4 months, 20 days ago)

Quote:

LewDoja said:
The only remark I have regarding the methodology is:
If using WA to clean up a culture- it is advisable to put to back on a nutritional medium for testing (any hidden contams that weren't able to show their ugly faces on WA).

Good luck



The grain jars I used started with a clean MYA plate.
I did 3 initial T1 transfers from MYA.
- 2 to MYA
- 1 to Water Agar and back to MYA
One MYA was inconsistent, one was clean, the post-WA one was incredibly slow and weak so I left it alone to monitor.
I used the clean MYA plate for WBS then expanded the WBS with G2G for the other 2 quarts of spawn.

I’m keep the WA in my tool box but so far I haven’t seen any improvement on the plates I’ve attempt to clean up with it.

If this grow flushes with consistent looking fruits I’m going to take another tissue clone to MYA but also intend to streak some diluted spores on water agar and test its efficacy there as well.


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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Spawning a Cloned Abort [Re: MicroMycelium]
    #28461141 - 09/06/23 10:21 PM (4 months, 19 days ago)

48 hours after spawning to bulk and the grains appeared to have colonized the compacted layer of substrate first, before the pseudo casing.. as intended.
Goal of the way it was set up was to have a solid lower sub which grows upright through the casing.. so far so good.



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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Spawning a Cloned Abort [Re: MicroMycelium]
    #28466405 - 09/12/23 12:02 PM (4 months, 13 days ago)

I’ve been trying to keep the majority of the initial updates in the first post but this is something worth mentioning.

This is my second time spawning a decent volume in one of these unmodded shoeboxes… first one developed a lot of stagnant water on the surface while colonizing which I assumed it was normal.
That tub contaminated.

This tub is showing an equal amount of moisture build up on the surface.. again prior to full visible colonization of the substrate surface.

This leads me to believe that even with the 6 predrilled holes in the tub, there is not enough FAE for adequate evaporation.. at least during the colonizing phase.

To attempt and avoid a repeat contamination, I used a paper towel to soak up the condensation on inside of the lid, interior walls, and any pooled on the surface just to be safe.
I did this last night at 6 days after spawning to bulk.
I’ll get more photos up tonight, 24 hours after the clean up and a week after spawning, to see how much moisture returned leaving it unmodded.


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 9 days, 21 hours
Trusted Cultivator
Re: Spawning a Cloned Abort [Re: MicroMycelium]
    #28466408 - 09/12/23 12:06 PM (4 months, 13 days ago)

how wet are you prepping your sub?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Spawning a Cloned Abort [Re: Camera93]
    #28466422 - 09/12/23 12:12 PM (4 months, 13 days ago)

Quote:

Camera93 said:
how wet are you prepping your sub?




I’ve been using a CVG bucket tek.
A 650g brick coir, 8 cups Verm, 1 cup gypsum, 4qt water.

I mix everything dry in an insulated bucket and bring the water to a boil.
Pour half the water in and let it sit for a with the lid on to start expanding.. then I mix everything while adding the rest of the hot water.
Place the lid back and let sit until room temp.

The stagnant water only has happened with these 12qt tubs. I ran 10 6qt sterilite shoeboxes in a row over multiple flushes and didn’t have a single issue until swapping to these.
The rim/lid on them is nearly air tight but I thought the holes on them would be enough. If it continues on my next few tries with the same sub/ratio I’ll probably start intentionally cracking the lid for more FAE


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InvisibleMicroMycelium
Curious Optimizer


Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: Spawning a Cloned Abort [Re: MicroMycelium] * 2
    #28477576 - 09/21/23 11:34 AM (4 months, 4 days ago)

15 days later and time to harvest!
Got a full even flush with relatively consistent characteristics. Nearly all caps are wavy, displaying the Tidal wave genetics.
I’m going to pull a second generation clone from that dark one on the right as the stipe looks to be 2-3x as thick as the rest.
.

First flush harvest weight with 3qt spawn is about 900g~



I’m going to dunk the tub for a few hours, drain any excess, and see how the characteristics continue on their second flush as the original multispore tubs(which provided the abort) looked different flush to flush.

Here is the fruit for the gen 2 clone.



Edited by MicroMycelium (09/21/23 12:30 PM)


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