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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
    #27959037 -

ReverendMyc said:
Ackchyually...

I used this tek and it works pretty well. Only a few plates were completely condensation free right after, but they do dry out in a few days.

Poisoned's glass petri no-pour tek


Great, I've read it and saved it, thank you!

Just one last thing: Anyone that can give me any input about the sterilization time using a 10 PSI PC? I've seen a chart with the times, but for the grains. I'm gonna search for it, but any info would be appreciated.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex]
    #27959049 -

A liquid media is much easier to sterilize than grain.

I do 15 min at 15 PSI. I know someone who uses a standard pot to sterilize and does 60 min. I would assume that 30-45 min would be enough at 10 PSI.

For grain you def want to refer to that chart.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex]
    #27959055 -

Nerex said:
I've finally decided to start doing agar, and I want to do it with the no pour way. I was thinking of recycling the half pints (no wide mouth, unfortunately) that I successfully used for the PF-TEK / shoeboxes I've done. I'd put microtape on the 4 holes of the caps. Here a generic picture of the jars:



What do you think? I wouldn't be as comfortable as having Petri dishes, but then I wouldn't be able to do no pours using them.

What are your thoughts?


Those will work this is another route for no pours https://www.shroomery.org/forums/showflat.php/Number/26373655/vc/1#26373655


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #27959066 -

Rusty2096 said:
A liquid media is much easier to sterilize than grain.

I do 15 min at 15 PSI. I know someone who uses a standard pot to sterilize and does 60 min. I would assume that 30-45 min would be enough at 10 PSI.

For grain you def want to refer to that chart.


Thanks for the input, Rusty. Considering the data on the chart (the usual time people use on 15 PSI * 2,67), 40 minutes should be enough if I take your 15 minutes as reference of the "right" time on 15 PSI. So 15 * 2,67 = 40,05.

I'll search a bit more on the matter, but I think this is a good starting point.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex]
    #27959073 -

Yup :super:

But I never said it was the "right" time. I said that I do 15. I know some here do as little as 10 min and others as much as 45 min at 15 PSI.

I don't get contam at 15 minutes so I just figured why do more. Also note that I boil small media bottles of 250ml content per bottle!

I'm sure you'll be fine at around 40 min


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie]
    #27959075 -

wild_aussie said:
Nerex said:
I've finally decided to start doing agar, and I want to do it with the no pour way. I was thinking of recycling the half pints (no wide mouth, unfortunately) that I successfully used for the PF-TEK / shoeboxes I've done. I'd put microtape on the 4 holes of the caps. Here a generic picture of the jars:


What do you think? I wouldn't be as comfortable as having Petri dishes, but then I wouldn't be able to do no pours using them.

What are your thoughts?


Those will work this is another route for no pours https://www.shroomery.org/forums/showflat.php/Number/26373655/vc/1#26373655


Yeah, my first search was for PP5 plastic containers to use but here where I live I just didn't find anything of which I'd sure that wouldn't melt / ruin inside a PC. All the brands and items you find around here are from the USA; for expample, I couldn't find any Ball jars or any wide mouth ones of the right capacity. : )


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex] * 4
    #27959207 -

Enigma "Krinkle" pinning on agar



--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex]
    #27959413 -

Nerex said:
I'd put microtape on the 4 holes of the caps.


Welcome.

If you're going to progress beyond pf tek, I'd recommend instead of tape on 4 holes, drill a 3/8 inch hole and use synthetic filters. Link in the article linked in my sig line "things I wish I'd known."

Also if you're PCing agar, let the PC shoot steam 10 min before putting the stopcock on. is noisy as hell, I wear headphones. Then put the stopcock on using tongs so you don't burn your hands. Then let it get to between 15 and 20 PSI (is easy to keep at 18 PSI on mine), turn the heat down to like 1/4 and it should stay at that heat. If not, ride it until it does. You'll get it after a few times.

THEN start counting your time....20 to 25 min for agar or LC, 2 hours for grain is what I do.

Then let it cool until pressure is zero before opening.

Don't put a jar in with the lid screwed tight, may implode, or suck in contams as it cools.

I use a jar with lid screwed tight, but has synthetic filter, which also works to equalize the pressure.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27959483 -

nektar61 said:
Nerex said:
I'd put microtape on the 4 holes of the caps.


Welcome.

If you're going to progress beyond pf tek, I'd recommend instead of tape on 4 holes, drill a 3/8 inch hole and use synthetic filters. Link in the article linked in my sig line "things I wish I'd known."

Also if you're PCing agar, let the PC shoot steam 10 min before putting the stopcock on. is noisy as hell, I wear headphones. Then put the stopcock on using tongs so you don't burn your hands. Then let it get to between 15 and 20 PSI (is easy to keep at 18 PSI on mine), turn the heat down to like 1/4 and it should stay at that heat. If not, ride it until it does. You'll get it after a few times.

THEN start counting your time....20 to 25 min for agar or LC, 2 hours for grain is what I do.

Then let it cool until pressure is zero before opening.

Don't put a jar in with the lid screwed tight, may implode, or suck in contams as it cools.

I use a jar with lid screwed tight, but has synthetic filter, which also works to equalize the pressure.


Hi nektar and thanks for your time! Fortunately, I already know most of the things you've told me, but it's goods to (re)read them in order to have them in my head for goods.

I would drill one single hole on them (in the middle) and use polyfill, but I wanted to recycle the PF tek caps (lots of them) that I have around, even it I know it's not ideal.  : )

Definitely gonna keep in mind the 10 minutes bit for the PC, before putting the weight and start the timer.


--------------------

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex] * 4
    #27959658 -



Psilocybe stunzii germ plates
:aweyeah:


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 4
    #27959734 -

I had a bunch of invitro pins in one of my pf jars and I did my best to remove them without disturbing anything and ended up transferring a little verm that was stuck to them and a couple has 2-3 little pins.

But the growth from them has been awesome to watch. I put these on these plates 2 days ago and each one has these nice white spikes of myc shooting off in every direction. These are Lipa Yai pins from this jar:



Here they are 2 days later plated up:









Give em some time and they'll hopefully grow into some decent cultures to take a transfers from. The other ones got eaten, lol. These little shits actually had enough kick for me to feel it too. Hopefully it's a good sign of their potency.

Going to go to grain with the transfers within the month and have a tub made up and fruits between Halloween and Thanksgiving. My cakes should be fruiting in the next week.

I used a lower nutrient and softer agar recipe this time. I ordered some yeast to start adding to my LME agar. Hopefully will help speed growth too.


--------------------


****FUGGIN' FROGZ****

Trade List

Edited by Cob (09/20/22 08:47 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 4
    #27959855 -

Finally got my ghost clone to stop throwing insane sectors. It was wild. Around T3 one of the plates was split evenly down the middle heavy rhizo and silky tomentose. Happy with how it’s going now.



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #27959901 -

Smellyhobbit said:
Finally got my ghost clone to stop throwing insane sectors. It was wild. Around T3 one of the plates was split evenly down the middle heavy rhizo and silky tomentose. Happy with how it’s going now.




That's what you get for doing transfers in your night club...

:cookiemonster:


--------------------


Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 2
    #27960334 -

Smellyhobbit said:
Finally got my ghost clone to stop throwing insane sectors. It was wild. Around T3 one of the plates was split evenly down the middle heavy rhizo and silky tomentose. Happy with how it’s going now.





Sometimes when I separate the tommy stuff away from the rhizo stuff the tommy stuff gets a lot more robust on it's own. The colonies have loving relationships and express loving dominant and submissive roles. The tommy one liked the rhizo one so it took the back seat on purpose.

They wall (even when sectoring) when they don't like each other.


--------------------
Question everything, it's very responsive.
culture storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #27960771 -

Hi folks!

A few weeks ago i boarded the agar ship and i would appreciate if you could have a look at some of my plates.
I started with two spore prints. #1 is a expensive GT print which shows a more or less weak growth from MS to T2. I expected more for the money.
#2 is a print from a MS-grow of mine. I label it "???", because i'm not completly sure what it was, but very likely EQ. I took this print just for fun and under not so sterile circumstances, but this little fucker shows pretty strong growth.

The plates to the right look ok to me. The left ones have a questionable sector, i guess you know what i mean. The sector on the lower left one appears somehow yellow, but this must be a light issue or the table shines through or whatever. In reality it isn't yellow, but looks like the one on the upper plate.



Is this mold or just thin myc?
It is not unorganized, grows as fast or slow as the mycelium, but i'm absolutely not sure about it. Maybe one of you has an idea.

I like this one.

These are the plates of the GT-print. i don't really like them as they are so slow and weak growing.
Would be cool if you could share your thoughts.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nerex] * 1
    #27960949 -

Nerex said:
nektar61 said:
Nerex said:
I'd put microtape on the 4 holes of the caps.


Welcome.

If you're going to progress beyond pf tek, I'd recommend instead of tape on 4 holes, drill a 3/8 inch hole and use synthetic filters. Link in the article linked in my sig line "things I wish I'd known."

Also if you're PCing agar, let the PC shoot steam 10 min before putting the stopcock on. is noisy as hell, I wear headphones. Then put the stopcock on using tongs so you don't burn your hands. Then let it get to between 15 and 20 PSI (is easy to keep at 18 PSI on mine), turn the heat down to like 1/4 and it should stay at that heat. If not, ride it until it does. You'll get it after a few times.

THEN start counting your time....20 to 25 min for agar or LC, 2 hours for grain is what I do.

Then let it cool until pressure is zero before opening.

Don't put a jar in with the lid screwed tight, may implode, or suck in contams as it cools.

I use a jar with lid screwed tight, but has synthetic filter, which also works to equalize the pressure.


Hi nektar and thanks for your time! Fortunately, I already know most of the things you've told me, but it's goods to (re)read them in order to have them in my head for goods.

I would drill one single hole on them (in the middle) and use polyfill, but I wanted to recycle the PF tek caps (lots of them) that I have around, even it I know it's not ideal.  : )

Definitely gonna keep in mind the 10 minutes bit for the PC, before putting the weight and start the timer.


Good.

I did this for nearly a year with various jar contams until I:
1. Started doing the 10 min steam shoot
2. Replaced micropore tape with synthetic filters.

I tried microfil, but it didn't work for me. Some people make it work, are able to have it work into a hard little pill, but for me it got poofy again and was problematic.

Some people make micropore tape work for bulk tek too, but not me.

Look into filters. About as cheap as microfil, and perfect. They work for many runs through the PC, and just never have to think of them again.

I sand a little where I put them before I place them, and wipe the dust from sanding off. I don't sand much, like 5 or 6 scrapes on the top of the jar with light sand paper. I also put high-heat silicon around them, but I've heard that isn't needed.

But that may contribute to them being able to go through PC more times. I've run them all through probably 20 times and they're still holding well.


--------------------
--I'm doing a poppy seed giveaway
--Why You're probably growing x7x+, not x7x!
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #27961075 -



Second step in reviving ESS. First step was finding an OG that had the spores.

Muda be praised! Amen


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Silandril]
    #27961122 -

I hope someone with experience answers your question. I've had plates that look like yours and I tossed mine.


--------------------
Avatar by Sol_Exposure

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SeaSalt]
    #27961268 -

Hey y'all! Just wanted some opinions on my first agar attempt... Be gentle, I'm a noob.

These are attempts at getting an isolated batch of myc from dried fruit (I know, not steril and risky). However i was able to get some decent growth without any contamination from my first inoculation to the agar. I then took pieces off the good looking myc and transfered to new plates. I'm now having random growth and im not sure why. All these transfers came from the same myc from the same good inoculation. I have two that look good and a few that look wild. Can someone please elaborate on what i am seeing here? And possible reasons for the randomness?
Thanks!












--------------------
Time gives construct to all things. Only through a shift in the perception of time may we bring about changes in matter.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Gumby-grows] * 1
    #27961327 -

1st two pic look like mold to me. Last 3 look like myc growth. Maybe a seasoned agar adept or TC can chime in.


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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