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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,115
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Saraph]
#27078943 - 12/08/20 05:30 PM (5 years, 10 months ago)

This PEU culture likes to blue quite noticeably:

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]

2
#27079160 - 12/08/20 07:56 PM (5 years, 10 months ago)

Looks like you buried a life saver lol

I’m still doing xfers so might throw up a few more pics. A t2 of lpeu. Sent a puck to LC

Edited by D3_Myc (12/08/20 08:05 PM)

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jay.ach
Traveler

Registered: 05/25/19
Posts: 538
Loc: USDA Zone 8
Last seen: 1 month, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27079385 - 12/08/20 09:51 PM (5 years, 10 months ago)

D3monic said:

Sand dollar!

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“One's destination is never a place, but a new way of seeing things.” - Henry Miller

LAGM v. 2.023
LAGM2021

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: jay.ach]
#27079394 - 12/08/20 09:54 PM (5 years, 10 months ago)

Lol, I was thinking the same thing

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Shu

Registered: 11/20/11
Posts: 888
Loc: PA, USA and FL, USA
Last seen: 1 month, 18 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Saraph]
#27080242 - 12/09/20 12:40 PM (5 years, 10 months ago)

Saraph said:

Could they be sclerotia?

No.

Saraph said:

Where should I be taking my best cuts from?

From the leading edge of the fastest colonies.

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adhoc

Registered: 05/03/20
Posts: 167
Last seen: 7 months, 6 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Shu]
#27080564 - 12/09/20 04:01 PM (5 years, 10 months ago)

This grew up inside the container and dropped spores. For fun and practice I was thinking of transferring those spores to a couple of new plates. Should I drag a sterile swab over the spores and streak, or cut out a tiny sample and drop it? Or is this a bad idea?

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Yeetusdeetus

Registered: 11/23/19
Posts: 1,242
Last seen: 3 months, 8 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: adhoc]
#27080595 - 12/09/20 04:17 PM (5 years, 10 months ago)

Why not just clone it lol

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Ababyphoenix
You

Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 19 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Yeetusdeetus]
#27080634 - 12/09/20 04:40 PM (5 years, 10 months ago)

:whathesaid:

:ytho:

--------------------

:sporedrop:

:aum:

Pho's MushiAventures: :aum:

:aum:

:sporedrop:

:heartpump:

:heartpump:

:peace:

:peace:

Edited by Ababyphoenix (12/09/20 04:41 PM)

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TheBoJim
Strangest

Registered: 07/23/20
Posts: 449
Last seen: 3 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ababyphoenix]
#27081159 - 12/09/20 10:23 PM (5 years, 10 months ago)

First time seeing this crap. Any one have any idea what it is? Showed up on two completely different plates.

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheBoJim]
#27081236 - 12/09/20 11:11 PM (5 years, 10 months ago)

That's pin mold. It grows fast and it's gross as hell.

I think it's not too late to take a wedge from 6 o'clock to try and salvage the culture.

--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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TheBoJim
Strangest

Registered: 07/23/20
Posts: 449
Last seen: 3 years, 6 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27081254 - 12/09/20 11:21 PM (5 years, 10 months ago)

Josex said:

That's pin mold. It grows fast and it's gross as hell.

I think it's not too late to take a wedge from 6 o'clock to try and salvage the culture.

Thank you Josex, doing some research on pin mold now. I have plenty plates with this culture so I just dumped it. I have been having bad luck lately and this is something new that I am not happy to see.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: TheBoJim]
#27081310 - 12/10/20 12:27 AM (5 years, 10 months ago)

By tomorrow night that will be full of black pinhead pube looking mold. I prefer molds that are super obvious like that.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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bigfootscreepyuncl
Stranger

Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27081389 - 12/10/20 02:13 AM (5 years, 10 months ago)

I just poured my first plates yesterday and inoculated my first BRF cakes and agar dishes today! I'm a little concerned I didn't use enough MS solution on the cakes but I got a nice fat drop zig-zagged onto 10 plates (I'll likely do another 10 tomorrow). Stay tuned in about a week for pictures of some contaminated plates from a noob :lol:

:lol:

You guys seriously make this shit look so easy! Cheers, mates!

--------------------

I

:heart:

5318008

NOT a virgin!

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,223
Loc: Gaming the system
Last seen: 2 hours, 3 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: bigfootscreepyuncl]
#27081419 - 12/10/20 03:06 AM (5 years, 10 months ago)

Save your syringe man ten plates is 8 or 9 more than you’ll need. Twenty would just be insane.
There are so many spores that as long as some of the liquid from the syringe got to the plate you should be good.

Plus it’s always smart to wait and see what happens with the first batch in case something went wrong you won’t repeat it and then a couple days later realize you ruined a ton of plates.

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Clc420
Stranger

Registered: 06/24/18
Posts: 276
Last seen: 3 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27081634 - 12/10/20 08:22 AM (5 years, 10 months ago)

I have 2 plates that I'm suspicious about. Would appreciate someone's input. These are t1 from Ms

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CapMeh
RX Jazzist

Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Clc420]
#27082033 - 12/10/20 01:55 PM (5 years, 10 months ago)

My first print to agar using an inoculation loop is a success! It was my first time making germ plates so I made three

I chose the one where I could see the most (I don't know what to call them, fans, sectors, little strong growth spots). Most of the germination was all wispy and cottony. It's crazy to think of all the genetics going on down there in a germ plate. I just decided to grab the most thick uniform looking growth and these are the results

This is my favorite one so far

And here's one with some tomentose growth on the top of the sample.

I'm happy to have revived my GT's and gained experience with germ plates :dancer:

:dancer:

GOD BLESS THIS COMMUNITY :phreaklove:

:phreaklove:

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CapMeh
RX Jazzist

Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 1 year, 2 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]
#27082038 - 12/10/20 01:57 PM (5 years, 10 months ago)

BTW does anyone know how to prevent the water deposits on the inside of the dish? Would using distilled water fuck with the agar recipe/ myc colonization?

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Edited by CapMeh (12/10/20 01:58 PM)

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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,564
Last seen: 1 year, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]
#27082171 - 12/10/20 02:59 PM (5 years, 10 months ago)

I use distilled water for agar and never have a problem with it.

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,115
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: kanemush]
#27082174 - 12/10/20 03:01 PM (5 years, 10 months ago)

Distilled is the way to go.

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27082244 - 12/10/20 03:51 PM (5 years, 10 months ago)

Some say not to use distilled but it’s all I use for just about everything. I’ve had mixed results using tap.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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