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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Vibetyme]
    #27833917 -

Question. Spore prints on a piece of foil in a 4oz mason jar with 3/4 6 gram silica gel packets. How long ROUGHLY do you think they will last in the fridge?

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Re: Ask Quick Questions, Get Quick Answers [Re: iceforeal] * 2
    #27833923 -

I've germinated 16 year old spores wrapped in tinfoil stored in the fridge.

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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #27833986 -

Aaaaand thats the answer! Thanks! I was hoping 1yr!

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 2
    #27834051 -

Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #27834084 -

Smellyhobbit said:
Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.


Either is fine really as long as they're sealed up good. Yeast spores can live in oak barrels for hundreds of years.

The important part is that a print is damp
after dropping and needs to dry to extend
shelf life. Then wrap it and seal it up good
in a jar or vacuum sealer.


--------------------

Links For Beginners And Beyond:mspoil:

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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: Vibetyme]
    #27834145 -

Post deleted by Nichrome

Reason for deletion: .


--------------------
Question everything, it's very responsive.
culture storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 2
    #27834147 -

Vibetyme said:
It is because there is no shaking involved so you aren't spreading the bacteria/mold from the syringe all around like you would a grain jar or bag.

This gives your mycelium a chance to take hold and possibly beat any contams.


:whathesaid:

Spore prints and spore syringes are rarely clean and often harboring other organisms. PF tek works quite well and is easy enough to pull off with basic gear and understanding. The materials can be found (or at least materials that will work) nearly everywhere in the world "Cakes" in general are a good thing to understand know how to pull off. It is a versatile skill set that is useful in many ways for a lot of species.

Smellyhobbit said:
Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.



They do not. I germinated wild spores taken on card paper and kept in a sandwich baggie for 18 years. They don't all fare that well.

IME spores that are allowed a little GE in storage and are not desicated too far last longer. The biggest culprit I've noticed in spores losing viability is dryness.


--------------------
Question everything, it's very responsive.
culture storage

Edited by Nichrome (06/24/22 09:05 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27834269 -

Bookmark this thread thanks


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms]
    #27834523 -

CowsPoopShrooms said:
"Quick question,....Why is PF Tek a good choice/option for MSS inoculation?"

My understanding is that it is a good option. I can understand how the verm can act as a filter to prevent contams from entering through the holes in the lids. But MSS Inoc would be below the dry verm layer, so what am I missing? What am I not understanding?


First reason is that BRF cakes are very resistant to bacterial contamination which is common in MSS.

Second reason is that the verm top layer isn't to protect against contams in the MSS, it's to protect against contams in the air that comes into the jars through the holes in the lid - especially trich and mold spores that float around in your ambient air. If you were to get a bunch of mold spores in your MSS, PF Tek isn't going to save you.


--------------------
* How eliminate condensation from no-pour agar plates
* Build a steam pasteurizer
* Learn about trich


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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
    #27834605 -

Alright. Quick question 1. Luer Lock on or off Pcing used syringe?
Quick question 2. Nichrome, are you really a torso? Legit got baked and read that for the millionth time and now in my mind there's mad scientist Reanimator torso mushroom shit going on everytime I see that!


--------------------
LAGM V2.022! GROW LOG!
https://www.shroomery.org/forums/showflat.php/Number/27601367#27601367

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27834965 -

PF Tek jars.

Edited by CowsPoopShrooms (10/14/22 02:01 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] * 1
    #27834989 -

I've reused single use syringes.  They will hold up in PC.  I first boil water run it through syringes 3 times fourth time leave water in push out air bubble wrap in foil place with needle facing up in a jar without lid works to hold up right.  I don't know if this is looked down upon or considered not sterile but I've had success with this process.


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] * 1
    #27834995 -

Yes they can be pc’ed (personally, I do 45mins @ 15psi).  although, some syringe barrels and plungers are not pc’able.. they get bent out of shape.

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Re: Ask Quick Questions, Get Quick Answers [Re: fahtster]
    #27835006 -

Sounds good

Edited by CowsPoopShrooms (10/14/22 02:02 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] * 1
    #27835055 -

CowsPoopShrooms said:
.
.
Quick Question: Can these single use needles (18g) be re-sterilized in a PC? Yes, they say, "single use". Can they be reused? Can they stand the heat of a PC? If yes, What's a good PC time?



Those pictured were used to inoc PF Tek jars.


You really don't need to pressure cook needles
just to push unsterilized spores
through them. Put the needle on the syringe,
Bring water to a boil in a frying pan,
Draw boiling water into the syringe a few
times and squirt it in the sink, on the last
draw leave the water in the syringe,
place on the cap, and wait for it to cool.

Always flame your needles before inoculation
and you'll be as good as any spore syringe
you buy from a vendor.

Here a tek for it
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681


--------------------

Links For Beginners And Beyond:mspoil:

Edited by rumfor69 (06/24/22 07:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
    #27836557 -

Quick question. How long is too long to take a tissue sample from a drying mushie? :mushroom2:


--------------------
You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #27836565 -

Quick question:  I’ve bought some some pre sterilized brf jars on Amazon ( I know I know I should make my own, and ive done several grows with the companies product, whose name I will not mention sorry mods!)

But my question is.  I’ve just birthed some cakes and I’ve decided to try to do my own jars this time.  My question is. Can I sterilize with the SHIPs in there or should I take them out?

After typing it out it sounds kinda stupid but I am curious

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Re: Ask Quick Questions, Get Quick Answers [Re: Tanavast] * 1
    #27836578 -

Yeah, I've re used/PCd jars with ships without any issues


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins]
    #27836599 -

Rotnpins said:
Yeah, I've re used/PCd jars with ships without any issues


Thanks brother!

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Re: Ask Quick Questions, Get Quick Answers [Re: Tanavast] * 1
    #27836643 -

For PF BRF TEK, when my jars are fully colonized, and after waiting 1 wk for consolidation, if I can't birth them right then, how long do I have before it's detrimental?

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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