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 iceforeal
the mussy bandit
Registered: 06/23/22
Posts: 29
Last seen: 2 years, 2 months
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 Re: Ask Quick Questions, Get Quick Answers  [Re: Vibetyme]
#27833917 - 06/24/22 03:39 AM (2 years, 3 months ago) |
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Question. Spore prints on a piece of foil in a 4oz mason jar with 3/4 6 gram silica gel packets. How long ROUGHLY do you think they will last in the fridge?
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DERRAYLD
Constructus
 
Registered: 05/13/02
Posts: 11,536
Loc: South Africa
Last seen: 23 minutes, 22 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: iceforeal]  1
#27833923 - 06/24/22 04:03 AM (2 years, 3 months ago) |
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I've germinated 16 year old spores wrapped in tinfoil stored in the fridge.
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iceforeal
the mussy bandit
Registered: 06/23/22
Posts: 29
Last seen: 2 years, 2 months
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
#27833986 - 06/24/22 06:03 AM (2 years, 3 months ago) |
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Aaaaand thats the answer! Thanks! I was hoping 1yr!
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Smellyhobbit
Actual Retard
Registered: 04/01/22
Posts: 14,161
Loc: Hole
Last seen: 1 hour, 40 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 2
#27834051 - 06/24/22 07:25 AM (2 years, 3 months ago) |
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Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.
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rumfor69
Bodhicitta Cultivator
Registered: 08/05/11
Posts: 7,336
Loc: In the Gills
Last seen: 3 hours, 32 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#27834084 - 06/24/22 08:19 AM (2 years, 3 months ago) |
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Quote:
Smellyhobbit said:
Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.
Either is fine really as long as they're sealed up good. Yeast spores can live in oak barrels for hundreds of years.
The important part is that a print is damp
after dropping and needs to dry to extend
shelf life. Then wrap it and seal it up good
in a jar or vacuum sealer.
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 Nichrome
Valence Electron
Registered: 12/17/18
Posts: 7,596
Loc: Zone 5
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Re: Ask Quick Questions, Get Quick Answers *DELETED* [Re: Vibetyme]
#27834145 - 06/24/22 09:01 AM (2 years, 3 months ago) |
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Post deleted by Nichrome
Reason for deletion: .
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discussions are a healthy alternative to arguments There is only one electron, and it's you.
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Nichrome
Valence Electron
Registered: 12/17/18
Posts: 7,596
Loc: Zone 5
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] 2
#27834147 - 06/24/22 09:02 AM (2 years, 3 months ago) |
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Quote:
Vibetyme said:
It is because there is no shaking involved so you aren't spreading the bacteria/mold from the syringe all around like you would a grain jar or bag.
This gives your mycelium a chance to take hold and possibly beat any contams.
Spore prints and spore syringes are rarely clean and often harboring other organisms. PF tek works quite well and is easy enough to pull off with basic gear and understanding. The materials can be found (or at least materials that will work) nearly everywhere in the world "Cakes" in general are a good thing to understand know how to pull off. It is a versatile skill set that is useful in many ways for a lot of species.
Quote:
Smellyhobbit said:
Do prints need to be kept in the fridge? Is that where people normally keep them? I haven’t taken any yet, but my mind defaulted to room temp for some reason.
They do not. I germinated wild spores taken on card paper and kept in a sandwich baggie for 18 years. They don't all fare that well.
IME spores that are allowed a little GE in storage and are not desicated too far last longer. The biggest culprit I've noticed in spores losing viability is dryness.
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discussions are a healthy alternative to arguments There is only one electron, and it's you.
Edited by Nichrome (06/24/22 09:05 AM)
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wild_aussie
Punk rocker til I die
Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 6 months, 20 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27834269 - 06/24/22 10:16 AM (2 years, 3 months ago) |
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Bookmark this thread thanks
--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC
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Hindsight
Mad Scientist
Registered: 01/24/21
Posts: 2,706
Last seen: 1 year, 5 months
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Quote:
CowsPoopShrooms said:
"Quick question,....Why is PF Tek a good choice/option for MSS inoculation?"
My understanding is that it is a good option. I can understand how the verm can act as a filter to prevent contams from entering through the holes in the lids. But MSS Inoc would be below the dry verm layer, so what am I missing? What am I not understanding?
First reason is that BRF cakes are very resistant to bacterial contamination which is common in MSS.
Second reason is that the verm top layer isn't to protect against contams in the MSS, it's to protect against contams in the air that comes into the jars through the holes in the lid - especially trich and mold spores that float around in your ambient air. If you were to get a bunch of mold spores in your MSS, PF Tek isn't going to save you.
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P.Nowhere
Registered: 11/29/21
Posts: 209
Loc: Masshole
Last seen: 1 month, 20 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Hindsight]
#27834605 - 06/24/22 02:32 PM (2 years, 3 months ago) |
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Alright. Quick question 1. Luer Lock on or off Pcing used syringe?
Quick question 2. Nichrome, are you really a torso? Legit got baked and read that for the millionth time and now in my mind there's mad scientist Reanimator torso mushroom shit going on everytime I see that!
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CowsPoopShrooms
StillUphill
Registered: 06/08/22
Posts: 505
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27834965 - 06/24/22 06:47 PM (2 years, 3 months ago) |
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PF Tek jars.
Edited by CowsPoopShrooms (10/14/22 02:01 PM)
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wild_aussie
Punk rocker til I die
Registered: 01/05/22
Posts: 1,000
Loc: Skatepark grinding hard
Last seen: 6 months, 20 days
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27834989 - 06/24/22 06:59 PM (2 years, 3 months ago) |
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I've reused single use syringes. They will hold up in PC. I first boil water run it through syringes 3 times fourth time leave water in push out air bubble wrap in foil place with needle facing up in a jar without lid works to hold up right. I don't know if this is looked down upon or considered not sterile but I've had success with this process.
--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC
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fahtster
Now With 33%More Faht
Registered: 06/17/06
Posts: 9,471
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27834995 - 06/24/22 07:02 PM (2 years, 3 months ago) |
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Yes they can be pc’ed (personally, I do 45mins @ 15psi). although, some syringe barrels and plungers are not pc’able.. they get bent out of shape.
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CowsPoopShrooms
StillUphill
Registered: 06/08/22
Posts: 505
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Re: Ask Quick Questions, Get Quick Answers [Re: fahtster]
#27835006 - 06/24/22 07:07 PM (2 years, 3 months ago) |
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Sounds good
Edited by CowsPoopShrooms (10/14/22 02:02 PM)
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rumfor69
Bodhicitta Cultivator
Registered: 08/05/11
Posts: 7,336
Loc: In the Gills
Last seen: 3 hours, 32 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: CowsPoopShrooms] 1
#27835055 - 06/24/22 07:45 PM (2 years, 3 months ago) |
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Quote:
CowsPoopShrooms said:
.
.
Quick Question: Can these single use needles (18g) be re-sterilized in a PC? Yes, they say, "single use". Can they be reused? Can they stand the heat of a PC? If yes, What's a good PC time?
Those pictured were used to inoc PF Tek jars.
You really don't need to pressure cook needles
just to push unsterilized spores
through them. Put the needle on the syringe,
Bring water to a boil in a frying pan,
Draw boiling water into the syringe a few
times and squirt it in the sink, on the last
draw leave the water in the syringe,
place on the cap, and wait for it to cool.
Always flame your needles before inoculation
and you'll be as good as any spore syringe
you buy from a vendor.
Here a tek for it
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26011681&page=0&vc=1#26011681
Edited by rumfor69 (06/24/22 07:57 PM)
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cozmyc
gentle modern ape
Registered: 06/20/21
Posts: 2,154
Last seen: 1 month, 8 days
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Re: Ask Quick Questions, Get Quick Answers [Re: rumfor69]
#27836557 - 06/25/22 06:49 PM (2 years, 3 months ago) |
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Quick question. How long is too long to take a tissue sample from a drying mushie? 
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You're conscious population 2 stardust
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Nothing matters so everything does 
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Tanavast
Stranger
Registered: 07/20/21
Posts: 82
Loc: Roshar
Last seen: 10 days, 18 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#27836565 - 06/25/22 06:56 PM (2 years, 3 months ago) |
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Quick question: I’ve bought some some pre sterilized brf jars on Amazon ( I know I know I should make my own, and ive done several grows with the companies product, whose name I will not mention sorry mods!)
But my question is. I’ve just birthed some cakes and I’ve decided to try to do my own jars this time. My question is. Can I sterilize with the SHIPs in there or should I take them out?
After typing it out it sounds kinda stupid but I am curious
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Rotnpins
🤮 Rotten-Pins 🍄
Registered: 01/11/22
Posts: 4,738
Loc: in (front of) the hood
Last seen: 1 year, 9 months
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Re: Ask Quick Questions, Get Quick Answers [Re: Tanavast] 1
#27836578 - 06/25/22 07:04 PM (2 years, 3 months ago) |
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Yeah, I've re used/PCd jars with ships without any issues
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Tanavast
Stranger
Registered: 07/20/21
Posts: 82
Loc: Roshar
Last seen: 10 days, 18 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Rotnpins]
#27836599 - 06/25/22 07:15 PM (2 years, 3 months ago) |
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Quote:
Rotnpins said:
Yeah, I've re used/PCd jars with ships without any issues
Thanks brother!
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LivingSpaces
Newb
Registered: 04/08/22
Posts: 14
Loc: Los Angeles
Last seen: 9 months, 25 days
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Re: Ask Quick Questions, Get Quick Answers [Re: Tanavast] 1
#27836643 - 06/25/22 07:42 PM (2 years, 3 months ago) |
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For PF BRF TEK, when my jars are fully colonized, and after waiting 1 wk for consolidation, if I can't birth them right then, how long do I have before it's detrimental?
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