Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar

Jump to first unread post. Pages: 1
OfflineMycoJunkie
Psilanthropist

Registered: 11/04/04
Posts: 963
Loc: .4merica
Last seen: 10 years, 10 months
Will spores germinate in agar?
    #3671848 - 01/24/05 12:40 PM (11 years, 10 months ago)


Germinate and grow myc, that is.

Could I throw some spores onto a fully prepared agar dish and expect them to colonize it after a while, rather than taking a cutting of my baby?

Just curious.


--------------------
:cussing::whip:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Will spores germinate in agar? [Re: MycoJunkie]
    #3671880 - 01/24/05 12:52 PM (11 years, 10 months ago)

spores germinate not in agar but on agar, they form mycelium,
thats called a tissue culture.

you usually dont throw them on but wipe them on, using an inoculation loop
for eample of a sterile needle.

anyway you shall work very sterile since on agar anything will germinate,
not just your spores.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 5 years, 6 months
Trusted Cultivator
Re: Will spores germinate in agar? [Re: ohmatic] * 1
    #3672053 - 01/24/05 01:35 PM (11 years, 10 months ago)

I have found quicker germination when working with agar if I break the surface of the agar with my loop and place some spore in the break when I'm streaking, this is usually if not always the place germination first occurs also. I've also noticed the myc was noticably more vigorous coming from this area. Just a tip I learned long ago and works very well for me. GL


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMycoJunkie
Psilanthropist

Registered: 11/04/04
Posts: 963
Loc: .4merica
Last seen: 10 years, 10 months
Re: Will spores germinate in agar? [Re: hyphae]
    #3673622 - 01/24/05 08:09 PM (11 years, 10 months ago)

Quote:

hyphae said:
I have found quicker germination when working with agar if I break the surface of the agar with my loop and place some spore in the break when I'm streaking, this is usually if not always the place germination first occurs also. I've also noticed the myc was noticably more vigorous coming from this area. Just a tip I learned long ago and works very well for me. GL




So basically you plant some spores beneath the surface of the agar.
If agar germinates spores/bacteria/etc., I'm assuming that you could introduce mycelium to the agar as well. Nifty... How long does one usually keep an agar culture for?


--------------------
:cussing::whip:


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Will spores germinate in agar? [Re: MycoJunkie]
    #3675622 - 01/25/05 01:33 AM (11 years, 10 months ago)

Not to long..they dry out. You can definatly keep them for a few months..I've kept them up to about 2 I think..somewhere around there. I dont know if I stored them well..just wrapped in clingfilm in a cool dark place..

One ended up fruiting...I cant seem to find pictures though..dont remember if I took any..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAeolus1369
Dr. Seahorse
Male

Registered: 05/20/02
Posts: 367
Last seen: 7 years, 9 months
Re: Will spores germinate in agar? [Re: hyphae]
    #3999446 - 04/01/05 11:32 AM (11 years, 8 months ago)

Quote:

hyphae said:
I have found quicker germination when working with agar if I break the surface of the agar with my loop and place some spore in the break when I'm streaking, this is usually if not always the place germination first occurs also. I've also noticed the myc was noticably more vigorous coming from this area. Just a tip I learned long ago and works very well for me. GL




Sorry to dredge up an old post, but I was doing some searching on the subject of spores to agar and came across it.

I was thinking an alternative to scratching the agar would be to streak the spores out and then just add 3 or so ml of top-agar (a less concentrated agar) or for that matter, just another layer of regular agar. This would provide the best rehydration potential since the spores would be 'submerged' in agar.

Of course care must be taken that the agar isn't too hot when you pour it, but this is a common technique in bacterial culturing so I'm sure the more resilient mushroom spores would survive the brief exposure to heat.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblederx
who run it
 User Gallery

Registered: 05/30/03
Posts: 2,459
Loc: dx/dt
Re: Will spores germinate in agar? [Re: Aeolus1369]
    #3999493 - 04/01/05 11:42 AM (11 years, 8 months ago)

that sounds like a pretty good technique as long as temperature is carefully monitored, however, spores suck. I find it much easier/successful to use mycelium when innoculating agar.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: Will spores germinate in agar? [Re: derx]
    #3999506 - 04/01/05 11:46 AM (11 years, 8 months ago)

ditto..... :thumbup:

Live myc transfer to agar begins colonizing much faster than spores.

Spores take time to germinate, then go.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAeolus1369
Dr. Seahorse
Male

Registered: 05/20/02
Posts: 367
Last seen: 7 years, 9 months
Re: Will spores germinate in agar? [Re: MycoJunkie]
    #3999565 - 04/01/05 11:59 AM (11 years, 8 months ago)

Ya, I don't doubt it...but if you've only got a spore print to work with, well, then you've only got a spore print to work with.

Anyone know about how long it takes for the spores to germinate and show growth?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Will spores germinate in agar? [Re: Aeolus1369]
    #4000049 - 04/01/05 01:47 PM (11 years, 8 months ago)

Only a few days...3-7ish..maybe more, maybe less.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblederx
who run it
 User Gallery

Registered: 05/30/03
Posts: 2,459
Loc: dx/dt
Re: Will spores germinate in agar? [Re: scatmanrav]
    #4000244 - 04/01/05 02:28 PM (11 years, 8 months ago)

if you only have a spore print to germinate. Do a MSI inoculation to grain, grow 1 flush and clone a few of the biggest mushrooms, then you already have isolated strains and you may select the phenotype.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Multi-spore vs. Agar/LC Inoc. of grain kompton 883 2 11/28/08 05:12 PM
by shroomophile
* spores germination on cardboard (pics) comario2 3,734 2 09/30/02 06:37 PM
by ChromeCrow
* single spore germination query unveiled 1,390 5 04/03/08 01:26 AM
by drftNV
* Cracked corn & spore germination Erik006 1,147 5 08/19/03 06:25 PM
by Erik006
* single spore germination witchdoctor 929 3 06/12/08 08:26 PM
by RogerRabbit
* Spore syringe - Distilled/Tap water - Spore germination veda_sticks 2,500 10 11/09/08 10:09 AM
by veda_sticks
* Does anyone have any information about woodlover spores germinating directly on wood chips? AsanteA 837 1 10/08/07 11:43 AM
by losfreddy
* Spore Germination in two days smurfyshmu 2,184 11 08/14/06 07:28 PM
by joeinc

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
4,414 topic views. 24 members, 60 guests and 18 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.003 seconds on 14 queries.